##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939539_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 932060 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.944079780271657 31.0 31.0 31.0 30.0 34.0 2 31.078516404523313 31.0 31.0 34.0 28.0 34.0 3 31.03932257579984 31.0 31.0 34.0 27.0 34.0 4 34.98907044610862 35.0 35.0 37.0 32.0 37.0 5 34.662856468467695 35.0 35.0 37.0 32.0 37.0 6 34.68879471278673 35.0 35.0 37.0 31.0 37.0 7 34.59825440422291 35.0 35.0 37.0 30.0 37.0 8 34.6554856983456 35.0 35.0 37.0 30.0 37.0 9 35.93643220393537 37.0 35.0 39.0 30.0 39.0 10-11 35.77920681072034 37.0 35.0 39.0 30.0 39.0 12-13 35.735135613587104 37.0 35.0 39.0 30.0 39.0 14-15 36.399510761109795 38.0 35.0 40.0 30.0 41.0 16-17 36.45102997661095 38.0 35.0 40.0 30.5 41.0 18-19 36.535588910585155 38.0 35.0 40.0 30.0 41.0 20-21 36.438999098770466 38.0 35.0 40.0 30.0 41.0 22-23 36.160849623414805 38.0 34.0 40.0 30.0 41.0 24-25 35.8553097440079 38.0 34.0 40.0 29.0 41.0 26-27 35.322162736304534 37.5 33.5 40.0 27.0 41.0 28-29 34.95910617342231 37.0 33.0 40.0 27.0 41.0 30-31 34.60239952363582 37.0 33.0 39.5 26.0 40.5 32-33 34.11145312533528 36.0 33.0 39.0 25.0 40.5 34-35 33.710052464433616 36.0 33.0 39.0 23.5 41.0 36-37 33.39876939252838 36.0 32.5 39.5 21.5 41.0 38-39 33.26060017595434 35.5 32.5 39.0 21.0 41.0 40-41 32.96858839559685 35.0 32.0 39.0 20.5 41.0 42-43 32.59859558397528 35.0 31.0 39.0 18.5 41.0 44-45 32.292908718322856 35.0 31.0 39.0 16.0 41.0 46-47 31.766106795699848 35.0 30.5 39.0 12.0 41.0 48-49 31.307486106044674 35.0 30.0 39.0 7.5 40.0 50-51 31.01607943694612 35.0 30.0 39.0 4.0 40.0 52-53 30.58922065103105 35.0 28.0 39.0 2.0 40.0 54-55 30.406833251078254 35.0 28.0 38.5 2.0 40.0 56-57 30.077528270712186 35.0 28.0 38.0 2.0 40.0 58-59 29.716098212561423 34.5 27.0 38.0 2.0 40.0 60-61 29.452383966697425 34.0 27.0 38.0 2.0 40.0 62-63 29.051556766731757 34.0 26.0 37.5 2.0 40.0 64-65 28.62927386648928 34.0 26.0 37.0 2.0 39.5 66-67 27.157883612642962 32.5 22.0 36.0 2.0 39.0 68-69 23.937513679376863 29.0 11.0 35.0 2.0 38.0 70-71 23.42914672875137 28.5 10.5 34.0 2.0 37.0 72-73 25.02988219642512 30.0 17.0 35.0 2.0 37.0 74-75 25.52800839001781 31.0 20.0 35.0 2.0 37.0 76-77 25.453195609724695 30.5 21.0 34.0 2.0 36.0 78-79 26.223223826792267 32.0 23.0 35.0 2.0 36.0 80-81 26.10411883355149 32.0 22.5 35.0 2.0 36.0 82-83 25.73214814496921 32.0 20.5 35.0 2.0 36.0 84-85 25.413228762096864 32.0 20.0 35.0 2.0 35.0 86-87 24.933253224041373 31.5 19.0 34.5 2.0 35.0 88-89 24.392954316245735 31.0 15.0 34.0 2.0 35.0 90-91 23.92717636203678 31.0 8.5 34.0 2.0 35.0 92-93 23.448013539900863 31.0 2.0 34.0 2.0 35.0 94-95 22.2633655558655 30.0 2.0 34.0 2.0 35.0 96-97 21.476346479840352 29.0 2.0 34.0 2.0 35.0 98-99 21.153302362508853 29.0 2.0 34.0 2.0 35.0 100-101 20.133663068901143 28.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3305478714009915 1101 2 0.22032473718266132 1101 3 0.24447015951709616 1101 4 0.2902291518164972 1101 5 0.2899092105293022 1101 6 0.5408356225635913 1101 7 0.5631717833117449 1101 8 0.6271710545611526 1101 9 0.8408583337291518 1101 10-11 0.8410301272789198 1101 12-13 0.766744964523312 1101 14-15 0.7599114950570822 1101 16-17 0.6753193974640013 1101 18-19 0.8244399288770978 1101 20-21 1.016601943651736 1101 22-23 1.0741365796805198 1101 24-25 0.8551193855315944 1101 26-27 1.0567448103640658 1101 28-29 0.8464900474357151 1101 30-31 1.0761134102949583 1101 32-33 1.1453301178562612 1101 34-35 1.1715501696279986 1101 36-37 1.121878013869349 1101 38-39 1.13519246946489 1101 40-41 1.4337360095040523 1101 42-43 1.4837311834976248 1101 44-45 0.8627482053336877 1101 46-47 0.9872137335404041 1101 48-49 0.9177615040542264 1101 50-51 0.7081710515560786 1101 52-53 1.0515464597106217 1101 54-55 1.0122113186690314 1101 56-57 1.082864896347889 1101 58-59 0.8400152535837115 1101 60-61 0.6099890430634396 1101 62-63 0.5749689290576434 1101 64-65 0.19754023797817766 1101 66-67 -2.5805847985373482 1101 68-69 -7.029117972502547 1101 70-71 -10.171695357920228 1101 72-73 -10.170088998976926 1101 74-75 -8.784858692711826 1101 76-77 -3.987365830317298 1101 78-79 -1.1448627925827388 1101 80-81 -0.6971096107916992 1101 82-83 -0.2294409238455657 1101 84-85 -0.4348224881935572 1101 86-87 -0.3761816295530487 1101 88-89 -0.5239464746715825 1101 90-91 -0.5295927341013389 1101 92-93 -1.0243954621876235 1101 94-95 -4.046268842291198 1101 96-97 -1.5012491444205267 1101 98-99 -0.7475278729262591 1101 100-101 -0.407735105288797 1102 1 0.262797857791103 1102 2 0.05128995031347827 1102 3 0.3456868833452873 1102 4 0.059146893664170364 1102 5 0.22971948871538217 1102 6 0.26101636185261157 1102 7 0.3034543245497048 1102 8 0.47503974637889 1102 9 0.7249837624405657 1102 10-11 0.4335093041529987 1102 12-13 0.2659589935940261 1102 14-15 0.07910755907798972 1102 16-17 0.43337985257858946 1102 18-19 0.23989871814161745 1102 20-21 0.32513466181821116 1102 22-23 0.20036827438355687 1102 24-25 0.5468826823904394 1102 26-27 0.4455860646511809 1102 28-29 0.44227302419002257 1102 30-31 0.1313797557683074 1102 32-33 0.32238112770981786 1102 34-35 0.6096183823777395 1102 36-37 0.646505102986886 1102 38-39 0.35449196862108323 1102 40-41 0.5077669856050946 1102 42-43 0.5088584359337958 1102 44-45 0.5462468987846094 1102 46-47 0.4833587332682008 1102 48-49 0.41656723653830596 1102 50-51 0.3831159315168833 1102 52-53 0.4367198496610811 1102 54-55 0.3240206169432973 1102 56-57 0.32351150204226187 1102 58-59 -0.007724144314924075 1102 60-61 0.0552160559656123 1102 62-63 0.04728982295497275 1102 64-65 0.061652329001095296 1102 66-67 -2.3993487486707217 1102 68-69 -7.038314853116628 1102 70-71 -10.608314117787394 1102 72-73 -8.729911253862877 1102 74-75 -6.961589870239184 1102 76-77 -3.7045217723936226 1102 78-79 -1.4060092893980283 1102 80-81 -0.9903652316747085 1102 82-83 -0.6981620028373428 1102 84-85 -0.6773744779589208 1102 86-87 -0.8277780692068184 1102 88-89 -0.532381201657266 1102 90-91 -0.8444234271167446 1102 92-93 -0.961789985569407 1102 94-95 -4.084699758508741 1102 96-97 -1.331544070654946 1102 98-99 -0.5642775598988798 1102 100-101 -0.7078671211851457 1103 1 0.5003331065623087 1103 2 0.3531154656221389 1103 3 0.41022814732475155 1103 4 0.3438281923904185 1103 5 0.5116474546321115 1103 6 0.5053743758735436 1103 7 0.7137565691216139 1103 8 0.6681331698093942 1103 9 0.8490245156948006 1103 10-11 0.6939857191237948 1103 12-13 0.8795675292531229 1103 14-15 1.0160575472920215 1103 16-17 0.723983053996399 1103 18-19 0.5470873940100773 1103 20-21 0.4056715562596054 1103 22-23 0.5653425635151521 1103 24-25 0.7242636659511987 1103 26-27 0.8815064177063618 1103 28-29 1.0301543220432379 1103 30-31 1.0102325665031415 1103 32-33 1.1136429245895911 1103 34-35 1.169987632353731 1103 36-37 1.1629257921603866 1103 38-39 1.036149184688412 1103 40-41 1.1557583512930947 1103 42-43 1.1552144987224366 1103 44-45 0.9672891056414272 1103 46-47 0.8088865918016843 1103 48-49 1.0300971990851195 1103 50-51 1.0082747146812885 1103 52-53 1.0237417540987153 1103 54-55 1.0949352516219797 1103 56-57 1.0219910031903048 1103 58-59 0.7883317037072182 1103 60-61 0.33069011374304225 1103 62-63 0.5676626305468808 1103 64-65 0.6861459689441745 1103 66-67 -2.139755189919864 1103 68-69 -6.519242867050259 1103 70-71 -10.4759206061804 1103 72-73 -7.959625204082446 1103 74-75 -6.1007330304497565 1103 76-77 -2.6190402283446623 1103 78-79 -0.10859696389581686 1103 80-81 0.49033142809498287 1103 82-83 0.782603408405592 1103 84-85 0.4994745001541041 1103 86-87 0.750595336413781 1103 88-89 0.9816904788469998 1103 90-91 0.9711251005104344 1103 92-93 0.9296132429124775 1103 94-95 1.456359603477587 1103 96-97 1.7939115442669653 1103 98-99 1.5593626887413699 1103 100-101 1.2345311484603165 1104 1 0.3593023165246656 1104 2 0.047955823140341636 1104 3 0.47891304830519843 1104 4 0.18116668673659575 1104 5 0.3709402820259555 1104 6 0.5295855979141066 1104 7 0.3195494265581189 1104 8 0.32766338692822927 1104 9 0.584474117286959 1104 10-11 0.6877563470997359 1104 12-13 0.6068486895533809 1104 14-15 0.7016516171870606 1104 16-17 0.881768688544831 1104 18-19 0.6367078128996368 1104 20-21 0.773784803386306 1104 22-23 0.6203822988867458 1104 24-25 0.8293639610017465 1104 26-27 1.1668589867754946 1104 28-29 1.121579935888576 1104 30-31 1.1238101624789039 1104 32-33 1.0741341906083974 1104 34-35 1.142506503888157 1104 36-37 1.5519819360952951 1104 38-39 1.361003108839654 1104 40-41 1.4411472877934486 1104 42-43 1.4170789778617703 1104 44-45 1.3009355151218358 1104 46-47 1.355363641844317 1104 48-49 1.2720238928850591 1104 50-51 1.2952527498069522 1104 52-53 1.398966151690665 1104 54-55 1.175172346191001 1104 56-57 1.3915816113275419 1104 58-59 1.5597555466167812 1104 60-61 1.4035430385805192 1104 62-63 1.205344981590624 1104 64-65 0.9548775678145702 1104 66-67 -1.8239968130246744 1104 68-69 -6.72004959154641 1104 70-71 -7.759891212862943 1104 72-73 -7.048085554622318 1104 74-75 -6.026972501024289 1104 76-77 -2.6564465031496205 1104 78-79 0.34734468377018857 1104 80-81 0.7726454719319733 1104 82-83 1.0061144783354194 1104 84-85 1.072018565358274 1104 86-87 1.2178577964754886 1104 88-89 1.1138523592434808 1104 90-91 1.1624267488510789 1104 92-93 0.9893359438846971 1104 94-95 1.9053524364583154 1104 96-97 1.966967810480778 1104 98-99 1.493080673542945 1104 100-101 1.0916519442007946 1105 1 0.44259303758628477 1105 2 0.4596213586448883 1105 3 0.5552618429202454 1105 4 0.3330554175726945 1105 5 0.5523786863745741 1105 6 0.5955431963794453 1105 7 0.6486649197603 1105 8 0.5118072831464318 1105 9 0.8377118751686794 1105 10-11 0.8422064628501644 1105 12-13 0.8155326056676415 1105 14-15 0.9978580403284738 1105 16-17 1.303273784972525 1105 18-19 1.1710269617698685 1105 20-21 1.2180804922640505 1105 22-23 1.1152254392407244 1105 24-25 1.2501051356128912 1105 26-27 1.3594711035362224 1105 28-29 1.4228641922811889 1105 30-31 1.3509599128484666 1105 32-33 1.3044016097302986 1105 34-35 1.6632393109986694 1105 36-37 1.3250971565789413 1105 38-39 1.4339801106092267 1105 40-41 1.4239427767808834 1105 42-43 1.6171831067584677 1105 44-45 1.7814965715342836 1105 46-47 1.460966148375615 1105 48-49 1.5682814507525222 1105 50-51 1.590693701594656 1105 52-53 1.471004133945364 1105 54-55 1.6014422693649486 1105 56-57 1.3895920056227666 1105 58-59 1.6346586230678462 1105 60-61 1.5552256809752372 1105 62-63 1.776042614207764 1105 64-65 1.7213793637281292 1105 66-67 2.4293742050522305 1105 68-69 -4.358919111220885 1105 70-71 -4.14575540522868 1105 72-73 -0.5828122317638567 1105 74-75 1.6670868834375732 1105 76-77 1.7653375099656614 1105 78-79 1.6195162361274988 1105 80-81 1.6563728525633756 1105 82-83 1.876532884357541 1105 84-85 1.7866712985868567 1105 86-87 1.8246147081859654 1105 88-89 1.9570361002600407 1105 90-91 1.7451565374632203 1105 92-93 1.9844254606460403 1105 94-95 2.672928061619082 1105 96-97 2.435169758558885 1105 98-99 1.8941520235307046 1105 100-101 1.7304269421089167 1106 1 0.049484377745340424 1106 2 0.16422655439181355 1106 3 0.13576360452437086 1106 4 0.12777533433985866 1106 5 0.26940082662506626 1106 6 0.24021247195423 1106 7 0.3563263213193366 1106 8 0.20709607587616574 1106 9 0.2434069403125605 1106 10-11 0.35391108085399736 1106 12-13 0.35425144261482444 1106 14-15 0.24419689887205465 1106 16-17 0.24203538121443557 1106 18-19 0.12654990597391702 1106 20-21 0.35744672857909876 1106 22-23 0.3130945682122146 1106 24-25 0.3583521621670087 1106 26-27 0.4247401127028638 1106 28-29 0.4622524979096525 1106 30-31 0.4028902881056098 1106 32-33 0.4307203497301444 1106 34-35 0.39393260271558717 1106 36-37 0.4140744212451821 1106 38-39 0.4974692887637602 1106 40-41 0.3616097744872562 1106 42-43 0.2955071000430891 1106 44-45 0.3452197435251776 1106 46-47 0.4329179229848741 1106 48-49 0.016632853336208342 1106 50-51 0.04734720549225102 1106 52-53 0.4585921156278374 1106 54-55 0.3054922839464602 1106 56-57 0.23425162587293968 1106 58-59 0.17889675884141454 1106 60-61 0.16179287809361753 1106 62-63 0.44425462543158645 1106 64-65 0.4137705738358797 1106 66-67 1.0903250180699722 1106 68-69 -5.715303546837603 1106 70-71 -6.017338109980681 1106 72-73 -2.4081912525168896 1106 74-75 0.32342678633259325 1106 76-77 0.5738364616732774 1106 78-79 0.40485029065446554 1106 80-81 0.2325257771414968 1106 82-83 0.4148334881109008 1106 84-85 0.4085372590354943 1106 86-87 0.42817791706412933 1106 88-89 0.20345229992426894 1106 90-91 0.03232214795560395 1106 92-93 0.2978969045308695 1106 94-95 1.4135210167789651 1106 96-97 1.340704230038238 1106 98-99 0.7818931110749432 1106 100-101 0.6174197419954162 1107 1 -0.036996581243840865 1107 2 -0.1032053434223208 1107 3 -0.021969721653089636 1107 4 -0.1344861259182153 1107 5 -0.016047578254216432 1107 6 0.025324486920226263 1107 7 -0.11824317626172132 1107 8 -0.1369368845850829 1107 9 0.16380232784140958 1107 10-11 0.21596629863531547 1107 12-13 0.1619140406566686 1107 14-15 0.19430960386863916 1107 16-17 0.17364951610775137 1107 18-19 0.4164715299992423 1107 20-21 0.2489351053971376 1107 22-23 0.28802920698120005 1107 24-25 0.3969584153775614 1107 26-27 0.4883887261247253 1107 28-29 0.19662090907588237 1107 30-31 0.279295746247584 1107 32-33 0.15479484619948636 1107 34-35 0.26620985700719046 1107 36-37 0.29157434457511755 1107 38-39 0.21350013696469006 1107 40-41 0.06422863225662212 1107 42-43 0.21326312056000063 1107 44-45 0.3211779276713358 1107 46-47 0.34240747285491935 1107 48-49 0.35049696714525425 1107 50-51 0.35988889484047704 1107 52-53 0.3578764005644679 1107 54-55 0.4607592299477332 1107 56-57 0.5467818147113128 1107 58-59 0.625092581856233 1107 60-61 0.39207908744889863 1107 62-63 0.4353470013602525 1107 64-65 0.5927434249656081 1107 66-67 0.9169581863070952 1107 68-69 -5.046611672331164 1107 70-71 -5.491009130229244 1107 72-73 -2.06396134582436 1107 74-75 0.31360487172770846 1107 76-77 0.3993473718236231 1107 78-79 0.2942503665578293 1107 80-81 0.31301976237484297 1107 82-83 0.3285676844936063 1107 84-85 0.3659623715488145 1107 86-87 0.5538756146620578 1107 88-89 0.5098196530435928 1107 90-91 0.5460206927577538 1107 92-93 0.44803366476057604 1107 94-95 1.3143387030297191 1107 96-97 1.3343990755097224 1107 98-99 1.1441639634160659 1107 100-101 0.889896050945115 1108 1 -0.09861850362525715 1108 2 -0.22776432874080044 1108 3 -0.1692148815564778 1108 4 -0.1840820995648187 1108 5 -0.031449897454010056 1108 6 0.00466160549785144 1108 7 0.08275835385373398 1108 8 -0.012972241633093518 1108 9 0.0020400394968405067 1108 10-11 -0.037602916959215804 1108 12-13 -0.03763324749822061 1108 14-15 -0.036838994368565636 1108 16-17 0.14549412719286892 1108 18-19 0.07480686554977467 1108 20-21 0.12650690069045822 1108 22-23 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0.8904768505964391 2316 96-97 0.7343354862960432 2316 98-99 -0.3068425346067123 2316 100-101 -0.2364080247260496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 21.0 4 81.0 5 264.0 6 791.0 7 1501.0 8 2415.0 9 3571.0 10 5300.0 11 7775.0 12 9924.0 13 11279.0 14 11878.0 15 14225.0 16 16327.0 17 17962.0 18 15317.0 19 11740.0 20 10755.0 21 10829.0 22 11638.0 23 13030.0 24 15652.0 25 18853.0 26 23258.0 27 28382.0 28 34261.0 29 40094.0 30 46789.0 31 53961.0 32 62210.0 33 72585.0 34 86432.0 35 95566.0 36 83262.0 37 65571.0 38 26973.0 39 1585.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.23825129927061 19.999699528581807 14.084383105350645 16.67766606679694 2 15.726900752349279 24.88998058765014 39.936215031747615 19.44690362825296 3 19.639293607707657 34.52803467587924 29.972855824732314 15.859815891680793 4 11.221917043967126 19.800012874707637 36.749243610926335 32.2288264703989 5 10.387980147158224 41.9730317212019 33.03217181763367 14.60681631400621 6 23.09829839280733 37.19631783361586 22.505096238439585 17.200287535137225 7 23.56318262772783 37.49254339849366 23.489260348046262 15.45501362573225 8 25.281741518786344 32.277321202497696 22.730725489775335 19.710211788940626 9 27.250177027230006 18.783232839087614 23.699118082526876 30.267472051155504 10-11 22.502521296912214 29.157135806707725 28.30649314421818 20.033849752161878 12-13 26.382314443276183 27.85614660000429 29.350095487414972 16.41144346930455 14-15 22.71302276677467 27.828787846275993 31.82375598137459 17.634433405574747 16-17 17.122522681040625 31.165777592762666 32.64664354931463 19.065056176882074 18-19 16.628426525014525 29.175949534814983 37.43303057727147 16.762593362899032 20-21 18.86461172027552 27.58266635195159 36.76447868162994 16.788243246142954 22-23 18.073407291376093 27.991062807115423 36.12412291054224 17.811406990966248 24-25 17.635774520953586 28.385833530030258 37.241057442653904 16.73733450636225 26-27 18.328224561373393 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17.237533580011846 52-53 17.77157428533083 31.177560059052478 33.676821903100254 17.374043752516442 54-55 16.877395695169614 30.922389272653394 34.06649679518319 18.1337182369938 56-57 17.861999388238072 31.755005446827695 32.553086446253495 17.82990871868074 58-59 17.745277511900113 30.803692146569894 32.88825054403578 18.562779797494215 60-61 17.774472975191447 30.588362159151995 33.472875403577554 18.164289462079 62-63 19.15633584985218 30.195382930910263 33.121754771649115 17.526526447588438 64-65 18.421536865337192 30.69159959464411 33.53429457828449 17.35256896173421 66-67 17.57776503125143 30.873666663973736 33.659016057930266 17.889552246844563 68-69 18.130012697798346 31.47037693919814 32.68691142947803 17.712698933525488 70-71 17.868523245283264 31.469126572019967 32.27376058878457 18.3885895939122 72-73 17.88147932205359 31.224562352793107 31.635813840661832 19.258144484491474 74-75 17.3255133729166 31.762859234803482 32.4194587230824 18.49216866919752 76-77 17.821688153587715 31.08200404861558 32.679881986021385 18.416425811775323 78-79 17.103181437030337 31.103038730794342 33.22442594270613 18.56935388946919 80-81 16.828747539013676 32.39875757071815 32.45594365078939 18.31655123947878 82-83 16.903096367186663 32.25779456258181 31.644046520610264 19.19506254962127 84-85 16.979969100701673 31.345353303435402 31.804819432225393 19.869858163637534 86-87 17.469012154809942 31.21631785112316 32.22735899766002 19.08731099640688 88-89 17.862815814757415 30.51648859212481 32.24712959073701 19.373566002380766 90-91 16.769292669188058 31.065207478340174 32.9978809581288 19.167618894342965 92-93 16.47213828154463 32.06078491224362 32.18717426978763 19.279902536424114 94-95 16.19273497060079 31.961764361210886 31.93929431142199 19.906206356766333 96-97 16.2215645638623 31.282460681617195 31.455620156601537 21.040354597918967 98-99 16.62569439153757 31.19356475962276 31.357310788030446 20.823430060809223 100-101 16.91792088121023 31.116770440916277 31.993120105237065 19.972188572636433 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15946.0 1 10623.5 2 10104.5 3 13672.0 4 10405.0 5 8039.0 6 8652.5 7 11591.0 8 13773.0 9 13529.0 10 11210.0 11 8365.5 12 7024.5 13 6429.5 14 6154.0 15 5683.0 16 5089.5 17 4866.5 18 4860.0 19 4893.0 20 4982.0 21 5283.0 22 5703.0 23 6195.0 24 6691.0 25 7278.0 26 8349.0 27 9631.0 28 10569.5 29 11539.0 30 12848.5 31 14171.5 32 15671.5 33 17486.5 34 19339.0 35 20608.0 36 21811.0 37 22990.0 38 22906.5 39 21982.5 40 21223.5 41 21335.5 42 21879.5 43 22497.0 44 23677.0 45 24611.5 46 26019.0 47 31076.0 48 42999.0 49 42160.0 50 29769.5 51 23721.0 52 19619.5 53 17512.0 54 16227.5 55 14823.0 56 13476.0 57 12317.5 58 11488.0 59 10771.5 60 9865.5 61 8671.5 62 7156.0 63 5301.0 64 3654.0 65 2638.0 66 1934.5 67 1447.5 68 1113.5 69 815.5 70 646.0 71 554.0 72 490.5 73 467.0 74 369.0 75 263.0 76 198.5 77 121.0 78 63.0 79 39.5 80 27.0 81 20.0 82 11.0 83 8.5 84 4.5 85 1.5 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0204922429886488 2 0.01920477222496406 3 0.0 4 0.0 5 2.1457846061412358E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.2915692122824715E-4 18-19 0.0012338261485312105 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.218676909211853E-4 28-29 0.018990193764349936 30-31 0.004130635366821878 32-33 0.00788575842756904 34-35 0.008583138424564941 36-37 0.09661395189150913 38-39 0.09972533957041392 40-41 0.11496041027401668 42-43 0.09752591034911916 44-45 0.09301976267622256 46-47 0.09457545651567495 48-49 0.09757955496427269 50-51 0.0953264811278244 52-53 0.07349312276033731 54-55 0.06131579512048581 56-57 0.03486899984979508 58-59 0.014591335321760402 60-61 0.008905006115486128 62-63 0.001984850760680643 64-65 0.00423792459712894 66-67 0.3972383752118962 68-69 0.7613780228740639 70-71 0.842327747140742 72-73 0.2637705727099114 74-75 0.13727657017788555 76-77 0.01464497993691393 78-79 0.008100336888183164 80-81 0.0016093384546059268 82-83 0.0 84-85 0.0 86-87 1.0728923030706179E-4 88-89 6.973799969959016E-4 90-91 0.003755123060747162 92-93 0.0020921399909877045 94-95 0.20739008218355043 96-97 0.21377379138682057 98-99 0.01384031070961097 100-101 0.0074029568911872625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 932060.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.623554326579644 #Duplication Level Percentage of deduplicated Percentage of total 1 82.55219447121804 42.61637696063293 2 10.650596006231426 10.996432430762807 3 2.8450675084055703 4.406174912488847 4 1.1578884799479303 2.3909727539485086 5 0.6230789464242656 1.6082774920240548 6 0.37158687642158 1.1509581181196085 7 0.292092674854485 1.0555203448122532 8 0.19458630034752092 0.8036189157758713 9 0.1524399431660269 0.7082542518814894 >10 1.000803306829937 9.89798015114203 >50 0.09726109525098166 3.453567772626128 >100 0.053429846399181004 5.091643766926935 >500 0.0037567860710217204 1.3672674813066288 >1k 0.0035480757337427353 3.71618776183979 >5k 6.261310118369533E-4 2.2128399945686006 >10k+ 0.0010435516863949222 8.523926891143525 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19312 2.071969615689977 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19089 2.0480441173315023 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15625 1.6763942235478402 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13937 1.49529000278952 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11150 1.1962749179237389 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7108 0.7626118490225952 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7044 0.7557453382829432 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6386 0.6851490247408965 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3853 0.413385404373109 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3845 0.41252709053065256 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3543 0.3801257429779199 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3133 0.33613715855202453 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2307 0.24751625431839153 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2074 0.22251786365684612 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1755 0.1882925991888934 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1675 0.17970946076432848 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1600 0.17166276849129883 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1563 0.16769306696993755 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1552 0.16651288543655987 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1456 0.15621311932708196 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1405 0.1507413685814218 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1355 0.1453769070660687 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1171 0.12563568868956934 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1168 0.12531382099864816 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1036 0.111151642598116 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 984 0.10557260262214879 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 1.0728923030706177E-4 0.0 0.0 5.364461515353089E-5 0.0 14-15 2.1457846061412355E-4 0.0 0.0 1.0728923030706177E-4 0.0 16-17 2.6822307576765446E-4 0.0 0.0 2.6822307576765446E-4 0.0 18-19 4.82801536381778E-4 0.0 0.0 4.291569212282471E-4 0.0 20-21 5.364461515353089E-4 0.0 0.0 8.046692273029634E-4 0.0 22-23 5.364461515353089E-4 0.0 0.0 0.0032723215243653844 0.0 24-25 5.364461515353089E-4 0.0 0.0 0.0065982876638842995 0.0 26-27 5.364461515353089E-4 0.0 0.0 0.008314915348797289 0.0 28-29 6.437353818423706E-4 0.0 0.0 0.011319013797395017 0.0 30-31 8.583138424564942E-4 0.0 0.0 0.019312061455271118 0.0 32-33 8.583138424564942E-4 0.0 0.0 0.03191854601635088 0.0 34-35 8.583138424564942E-4 0.0 0.0 0.04870931055940604 0.0 36-37 8.583138424564942E-4 0.0 0.0 0.07258116430272729 0.0 38-39 8.583138424564942E-4 0.0 0.0 0.10691371800098706 0.0 40-41 8.583138424564942E-4 0.0 0.0 0.14779091474797762 0.0 42-43 0.0012338261485312105 0.0 0.0 0.18850717764950753 0.0 44-45 0.001502049224298865 0.0 0.0 0.23474883591185117 0.0 46-47 0.0017166276849129884 0.0 0.0 0.28984185567452736 0.0 48-49 0.0018239169152200502 0.0 0.0 0.35442997231937856 0.0 50-51 0.0018239169152200502 0.0 0.0 0.4222367658734416 0.0 52-53 0.0018239169152200502 0.0 0.0 0.49138467480634296 0.0 54-55 0.0018239169152200502 0.0 0.0 0.5672918052485891 0.0 56-57 0.0018239169152200502 0.0 0.0 0.6476514387485783 0.0 58-59 0.0018239169152200502 0.0 0.0 0.7392764414308092 0.0 60-61 0.0018239169152200502 0.0 0.0 0.8432933502135056 0.0 62-63 0.0018239169152200502 0.0 0.0 0.9421067313263094 0.0 64-65 0.0018239169152200502 0.0 0.0 1.0373259232238268 0.0 66-67 0.0018239169152200502 0.0 0.0 1.1362465935669377 0.0 68-69 0.0018239169152200502 0.0 0.0 1.2508314915348797 0.0 70-71 0.0018239169152200502 0.0 0.0 1.4043087354891317 0.0 72-73 0.0018775615303735812 0.0 0.0 1.5796193378108705 0.0 74-75 0.001931206145527112 0.0 0.0 1.7677510031543033 0.0 76-77 0.001931206145527112 0.0 0.0 1.971707829968028 0.0 78-79 0.001931206145527112 0.0 0.0 2.2047936828101196 0.0 80-81 0.002360363066755359 0.0 0.0 2.465238289380512 0.0 82-83 0.002360363066755359 0.0 0.0 2.724502714417527 0.0 84-85 0.0024140076819088897 0.0 0.0 3.0250734931227603 0.0 86-87 0.0025212969122159518 0.0 0.0 3.328970237967513 0.0 88-89 0.0025749415273694826 0.0 0.0 3.6307748428212774 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 130 0.0 58.336212 2 ATCACGC 140 0.0 54.15191 3 TACCTGG 1200 0.0 53.71793 2 TATAACG 130 0.0 51.044186 2 GTACCTG 1420 0.0 45.736588 1 ACCTGGG 1380 0.0 45.666145 3 TCACGCA 180 0.0 42.11815 4 ATAACGC 170 0.0 39.02123 3 TAACGCA 210 0.0 38.3576 4 CGCATAT 30 0.0036823694 32.12632 64-65 CCTGGGA 1365 0.0 30.200102 4 AAGTACT 435 0.0 27.231562 4 GTATGCG 70 0.008492382 27.075954 9 GTACATG 11965 0.0 26.704147 1 AGTACTC 355 0.0 26.694603 5 GTACGTG 125 7.5037296E-6 26.54725 1 TGGGAGT 680 0.0 26.478691 6 TACATGG 11820 0.0 26.425888 2 ATGGGAG 2060 0.0 26.221617 5 TACGTGG 110 9.613973E-5 25.85355 2 >>END_MODULE