FastQCFastQC Report
Fri 27 May 2016
SRR939538_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939538_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924440
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155551.682640301155294No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117541.2714724590022068No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA111931.210787071091688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83850.9070356107481286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70160.7589459564709445No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA49240.5326467915711133No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48470.5243174246030029No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA39540.4277184024923197No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23840.2578858552204578No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21790.23571026783782614No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18480.19990480723465018No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA13910.15046947341092987No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT13810.1493877374410454No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG12220.13218813551988232No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10600.11466401280775387No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG10280.11120245770412358No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT9900.10709186101856259No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9890.10698368742157414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG10400.062.204722
GTACCTG13800.049.0614621
ATAACGC1200.048.1466833
TATAACG1051.4551915E-1146.675982
ACCTGGG13300.045.6126483
CCTGGGA13250.032.385174
GTACATG106250.028.6749821
TACATGG106650.028.07782
CATGGGT8950.027.7012794
ACATGGG111250.026.615923
ATGGGAT26600.026.4851635
GTACCCG1358.313869E-626.1986581
TATCACG950.001021785425.7946192
TACCCGG950.001021785425.7946192
ATGGGTA4900.024.7890685
CATGGGG25150.024.4552024
CATGGGA81350.024.4398234
TGGGATG11900.024.0743896
TACACGG2251.9572326E-923.9603372
GGGATTC3950.023.3564437
Produced by FastQC (version 0.11.5)