##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939538_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 924440 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.84484877331141 31.0 31.0 31.0 28.0 34.0 2 30.97200250962745 31.0 31.0 33.0 28.0 34.0 3 30.940884210981782 31.0 31.0 34.0 27.0 34.0 4 34.85334256414694 35.0 35.0 37.0 32.0 37.0 5 34.49274155164208 35.0 35.0 37.0 30.0 37.0 6 34.613408117346715 35.0 35.0 37.0 30.0 37.0 7 34.48253861797412 35.0 35.0 37.0 30.0 37.0 8 34.41556834407858 35.0 35.0 37.0 30.0 37.0 9 35.83402167798884 37.0 35.0 39.0 30.0 39.0 10-11 35.637823979922985 37.0 35.0 39.0 30.0 39.0 12-13 35.623317359698845 37.0 35.0 39.0 30.0 39.0 14-15 36.2256609406776 38.0 34.5 40.0 29.5 41.0 16-17 36.18479349660335 38.0 34.5 40.0 29.5 41.0 18-19 36.33450467309939 38.0 35.0 40.0 30.0 41.0 20-21 36.22731816018346 38.0 34.5 40.0 29.5 41.0 22-23 35.92528449656008 38.0 34.0 40.0 29.0 41.0 24-25 35.67322487127342 38.0 34.0 40.0 29.0 41.0 26-27 35.05271407554844 37.0 33.5 40.0 26.5 41.0 28-29 34.76809690190818 37.0 33.0 40.0 26.0 41.0 30-31 34.328827181861456 36.5 33.0 39.0 25.0 40.0 32-33 33.745850460819526 36.0 33.0 39.0 24.0 40.0 34-35 33.59588832157847 36.0 33.0 39.0 23.5 40.5 36-37 33.20631192938428 35.5 32.0 39.0 21.0 41.0 38-39 32.9223492060058 35.0 31.0 39.0 21.0 41.0 40-41 32.690502899052404 35.0 31.0 39.0 19.0 40.0 42-43 32.4595225217429 35.0 31.0 39.0 18.5 40.0 44-45 31.94569901778374 35.0 31.0 39.0 15.0 40.0 46-47 31.328804465406083 35.0 30.0 39.0 9.0 40.0 48-49 30.788527649171392 35.0 29.0 39.0 6.5 40.0 50-51 30.52808078404223 35.0 28.0 39.0 2.0 40.0 52-53 30.356923110207262 35.0 28.0 38.0 2.0 40.0 54-55 30.051362987322054 35.0 27.5 38.0 2.0 40.0 56-57 29.61952262991649 34.5 27.0 38.0 2.0 40.0 58-59 29.22758480810004 34.0 26.0 38.0 2.0 40.0 60-61 28.87946324261174 34.0 26.0 38.0 2.0 40.0 62-63 28.691752304097616 34.0 26.0 37.0 2.0 40.0 64-65 28.23088680714811 33.0 26.0 37.0 2.0 39.0 66-67 27.47445264159924 33.0 23.5 36.0 2.0 39.0 68-69 24.451866535416038 30.0 10.5 35.0 2.0 38.0 70-71 23.301228311193803 28.5 9.5 35.0 2.0 37.0 72-73 24.17441910778417 29.5 11.0 34.5 2.0 37.0 74-75 24.804407533209293 30.5 16.5 34.0 2.0 36.5 76-77 24.375696097096622 29.5 17.0 34.0 2.0 36.0 78-79 25.385381960970967 31.0 20.0 34.5 2.0 36.0 80-81 25.41732724676561 32.0 20.0 35.0 2.0 36.0 82-83 25.118629116005366 31.0 19.5 34.5 2.0 35.5 84-85 24.78016637099217 31.0 18.0 34.0 2.0 35.0 86-87 24.299398554800746 31.0 13.5 34.0 2.0 35.0 88-89 23.7615983730691 31.0 7.5 34.0 2.0 35.0 90-91 23.143566916187098 30.0 2.0 34.0 2.0 35.0 92-93 22.642724243866557 30.0 2.0 34.0 2.0 35.0 94-95 21.901897364891177 29.0 2.0 34.0 2.0 35.0 96-97 21.238721820777982 29.0 2.0 34.0 2.0 35.0 98-99 20.445878045086754 29.0 2.0 33.5 2.0 35.0 100-101 19.288574163818097 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3719309434708471 1101 2 0.048251805619525356 1101 3 0.2591005357743619 1101 4 0.39561720694613456 1101 5 0.6748675522395828 1101 6 0.3791043654688764 1101 7 0.487286283507693 1101 8 0.8368945252398774 1101 9 0.9035179085692917 1101 10-11 0.9051704020377329 1101 12-13 0.7636056947995158 1101 14-15 0.776930682514049 1101 16-17 0.5994748618460406 1101 18-19 0.4965013326023282 1101 20-21 0.7846281311694696 1101 22-23 0.917769754862654 1101 24-25 0.9471329606354217 1101 26-27 0.8743471827476768 1101 28-29 0.7428415632965724 1101 30-31 0.7170499038997278 1101 32-33 1.2431208476906548 1101 34-35 1.1215135418661148 1101 36-37 1.3606875696909455 1101 38-39 1.4434793772016832 1101 40-41 1.3430398907735324 1101 42-43 1.3664330874794572 1101 44-45 1.5873990980502946 1101 46-47 1.77838441289121 1101 48-49 1.7185487181971943 1101 50-51 1.2956783549495015 1101 52-53 1.213922233092262 1101 54-55 1.3027669114416973 1101 56-57 1.6429757406475325 1101 58-59 1.4274019440772818 1101 60-61 1.458969254580353 1101 62-63 1.0811981762304903 1101 64-65 1.0138377879269065 1101 66-67 0.8443799635794527 1101 68-69 -5.9385847992846585 1101 70-71 -6.253983854160953 1101 72-73 -3.3630530960287537 1101 74-75 -1.4094405084229784 1101 76-77 0.9149109112087359 1101 78-79 1.3281582169145985 1101 80-81 1.207755026340326 1101 82-83 0.9184447718262021 1101 84-85 0.733964135998658 1101 86-87 0.48365002296543125 1101 88-89 0.618347882835856 1101 90-91 0.5535116790325958 1101 92-93 0.4349407227756359 1101 94-95 0.5382747209712093 1101 96-97 0.4582743298381864 1101 98-99 0.05066466988148122 1101 100-101 0.11231326311978762 1102 1 0.40980830766000764 1102 2 0.37936357298375967 1102 3 0.4257705582808633 1102 4 0.20118096566572063 1102 5 0.48215173817995804 1102 6 0.5554157158140782 1102 7 0.5930455758281923 1102 8 0.6711853915387422 1102 9 0.705789588413225 1102 10-11 0.4673703340260502 1102 12-13 0.43301128607611616 1102 14-15 0.5038553685193037 1102 16-17 0.616444225265603 1102 18-19 0.37562230391660734 1102 20-21 0.5436950510901752 1102 22-23 0.3332272005763528 1102 24-25 0.44559430807223066 1102 26-27 0.3976025934801086 1102 28-29 0.36532188688901357 1102 30-31 0.3898918751647926 1102 32-33 0.5191800626031196 1102 34-35 0.8578345794023008 1102 36-37 0.8637878063895101 1102 38-39 0.9540525099005421 1102 40-41 0.9587531633437933 1102 42-43 0.7199562718582513 1102 44-45 0.892402431517862 1102 46-47 0.8091138382371916 1102 48-49 0.6571698483545632 1102 50-51 0.407699085090762 1102 52-53 0.07028333935604536 1102 54-55 0.27734362817749414 1102 56-57 0.5041178000593192 1102 58-59 0.381980651944712 1102 60-61 0.4927491383340623 1102 62-63 0.3489214849990745 1102 64-65 0.25114935752031187 1102 66-67 0.06209734369766551 1102 68-69 -5.762291346751891 1102 70-71 -6.861638636680162 1102 72-73 -1.1578748114445894 1102 74-75 0.3513233478396849 1102 76-77 0.40791118862482634 1102 78-79 0.12135458723823689 1102 80-81 0.10371951708044591 1102 82-83 0.06481475987941465 1102 84-85 -0.3953923034105351 1102 86-87 -0.5781740549616465 1102 88-89 -0.45975201827873136 1102 90-91 -0.6269989455563163 1102 92-93 -0.263358649458592 1102 94-95 -0.6165536613797578 1102 96-97 -1.148652794744013 1102 98-99 -0.9767083563253927 1102 100-101 -0.7141204635179754 1103 1 0.4540027539453817 1103 2 0.2542680287683261 1103 3 0.30057449934167835 1103 4 0.27605559840039007 1103 5 0.5265251227647596 1103 6 0.2883646631708743 1103 7 0.439771667683182 1103 8 0.40378308964111653 1103 9 0.4959037996487865 1103 10-11 0.5050837897443401 1103 12-13 0.5158900940377649 1103 14-15 0.6201757564398633 1103 16-17 0.5252431295446556 1103 18-19 0.5830730958842665 1103 20-21 0.5890564622727226 1103 22-23 0.5419046035409849 1103 24-25 0.7145667383194336 1103 26-27 0.9500652042119881 1103 28-29 0.6122313352730444 1103 30-31 0.40987718455132693 1103 32-33 0.6661469479721518 1103 34-35 0.6484011068947311 1103 36-37 1.0898537042842307 1103 38-39 0.9853181117738714 1103 40-41 0.89379469470218 1103 42-43 0.589193243603674 1103 44-45 0.8919473538674758 1103 46-47 0.9669871234118226 1103 48-49 0.8978689227354586 1103 50-51 0.7232904651247836 1103 52-53 0.80093038908322 1103 54-55 0.46942339931320376 1103 56-57 0.6736253883686878 1103 58-59 0.7165720761610288 1103 60-61 0.7186090362729587 1103 62-63 0.20174694283812045 1103 64-65 0.43324227288912454 1103 66-67 0.4304015167155377 1103 68-69 -6.189262429439058 1103 70-71 -6.9559065360328916 1103 72-73 -0.7785278257817403 1103 74-75 0.6146327122773592 1103 76-77 0.9118472789334398 1103 78-79 0.6229020721610254 1103 80-81 0.4580151408240134 1103 82-83 0.5063201611651174 1103 84-85 0.27499893082190496 1103 86-87 0.20473678516471594 1103 88-89 0.05982129119616175 1103 90-91 0.0637757910067478 1103 92-93 0.4818823649669248 1103 94-95 0.5854312282749596 1103 96-97 0.5608077394199462 1103 98-99 0.5837755502732342 1103 100-101 0.6021540852703282 1104 1 0.35693799332904064 1104 2 0.38513038516209974 1104 3 0.12484361887638684 1104 4 0.3980541816453922 1104 5 0.5949522584163844 1104 6 0.39451156894601525 1104 7 0.6338564492845435 1104 8 0.7008435893401881 1104 9 0.43294661654118727 1104 10-11 0.5737607216708582 1104 12-13 0.4719190256660255 1104 14-15 0.7418308032300587 1104 16-17 0.6078122496119747 1104 18-19 0.8548466494360127 1104 20-21 0.7213153343014014 1104 22-23 0.616477047123773 1104 24-25 0.7643566218366047 1104 26-27 0.7282322641258077 1104 28-29 0.504175005946756 1104 30-31 0.7891084085530693 1104 32-33 1.1176204264252263 1104 34-35 0.6634858192561879 1104 36-37 0.8556135057254792 1104 38-39 1.042346761116363 1104 40-41 0.8868541930856964 1104 42-43 0.5969720806928294 1104 44-45 0.6475672374379151 1104 46-47 0.7687729803621828 1104 48-49 0.8024781699911614 1104 50-51 0.4335880369345517 1104 52-53 0.5263765870134804 1104 54-55 0.41282776568817425 1104 56-57 0.5075753119384814 1104 58-59 0.5469593402793116 1104 60-61 0.5558020504990964 1104 62-63 0.3278040497818182 1104 64-65 0.29132603187033723 1104 66-67 0.30843755342334234 1104 68-69 -6.272756168553748 1104 70-71 -3.798697862935793 1104 72-73 0.2982613600102262 1104 74-75 0.8007660896401667 1104 76-77 1.0201134584352332 1104 78-79 0.30994994889269023 1104 80-81 0.10245970362727164 1104 82-83 0.027875076399492826 1104 84-85 0.185602756060387 1104 86-87 0.3830662925494188 1104 88-89 0.32810408827530324 1104 90-91 0.16898209929476238 1104 92-93 0.3144906322116583 1104 94-95 0.49074832041196714 1104 96-97 0.18214481525894755 1104 98-99 0.1970562967859415 1104 100-101 0.38578896726955136 1105 1 0.05631320183312383 1105 2 0.2446542339106621 1105 3 0.1801821471361542 1105 4 0.25297549580802325 1105 5 0.31935001625098636 1105 6 0.29842465002887764 1105 7 0.046333127716678746 1105 8 0.38969783623373644 1105 9 0.33366160535919676 1105 10-11 0.30718438961292094 1105 12-13 0.504363290722587 1105 14-15 0.541729691620148 1105 16-17 0.7687892431169487 1105 18-19 0.5371492346807187 1105 20-21 0.5354975246548079 1105 22-23 0.5667403285764934 1105 24-25 0.6271655682577446 1105 26-27 0.900071033323627 1105 28-29 0.6775603091374265 1105 30-31 0.6043856314640124 1105 32-33 1.0602637845662528 1105 34-35 1.220998453059174 1105 36-37 1.2670147149461997 1105 38-39 1.1157566729371524 1105 40-41 1.1894626049126629 1105 42-43 1.0600158902323287 1105 44-45 1.2258489372661607 1105 46-47 1.3615660495834838 1105 48-49 1.2244348340905695 1105 50-51 1.2822784253596424 1105 52-53 1.2275588309086878 1105 54-55 1.3914192871398967 1105 56-57 1.18727133872585 1105 58-59 1.3534804213292233 1105 60-61 1.2557404774413286 1105 62-63 0.8536832677422055 1105 64-65 0.7559858767638907 1105 66-67 1.0387774202278415 1105 68-69 -1.9505931626353217 1105 70-71 -2.433144384984711 1105 72-73 1.4163812400766211 1105 74-75 1.9912044527441104 1105 76-77 1.6171157360205797 1105 78-79 1.529627277239772 1105 80-81 1.381711539492855 1105 82-83 1.1804171787796314 1105 84-85 1.0718496207767565 1105 86-87 1.28933521236441 1105 88-89 1.104837400741065 1105 90-91 1.1222195241818085 1105 92-93 0.9208817714573776 1105 94-95 1.1647972328958538 1105 96-97 1.1457214685676256 1105 98-99 1.2075000346618125 1105 100-101 1.2012479233694044 1106 1 0.2196033036037086 1106 2 0.27933180937369784 1106 3 0.32321594847964263 1106 4 0.29835936984478195 1106 5 0.4586348626890171 1106 6 0.37518298298467556 1106 7 0.294080253964097 1106 8 0.5411284316596152 1106 9 0.48279402244149594 1106 10-11 0.5539234887105309 1106 12-13 0.3239027489620554 1106 14-15 0.6523089314091095 1106 16-17 0.4786159118703708 1106 18-19 0.4509809322437448 1106 20-21 0.6241637574957011 1106 22-23 0.7100680949365952 1106 24-25 0.576665879211987 1106 26-27 0.6310806873201074 1106 28-29 0.602852523749398 1106 30-31 0.707216241716857 1106 32-33 0.9019540988565566 1106 34-35 0.6706068772419442 1106 36-37 0.6831821730703425 1106 38-39 0.858725194483597 1106 40-41 0.5928562276736855 1106 42-43 0.47744003593188467 1106 44-45 0.7976181021378146 1106 46-47 1.0271656558453905 1106 48-49 1.0054283225852636 1106 50-51 0.7753988202509312 1106 52-53 0.7339045625984255 1106 54-55 0.7556558430025184 1106 56-57 0.8966902661602525 1106 58-59 1.023561578326106 1106 60-61 1.3711631898683763 1106 62-63 1.0256677323860899 1106 64-65 0.8950900196628098 1106 66-67 1.0103704120194728 1106 68-69 -2.568280074962704 1106 70-71 -3.1364394702603065 1106 72-73 0.9447516957172475 1106 74-75 1.4869308912678392 1106 76-77 1.682371851110716 1106 78-79 1.4238868766648167 1106 80-81 1.015989713800213 1106 82-83 0.9353013225846425 1106 84-85 0.8870269676575369 1106 86-87 0.6770642579614226 1106 88-89 0.8230176182854763 1106 90-91 0.9283920687759419 1106 92-93 0.8266981601872452 1106 94-95 0.945987384501489 1106 96-97 0.6994388132243579 1106 98-99 0.5744723700311063 1106 100-101 0.6716485806978305 1107 1 -0.006849686017382339 1107 2 0.008543411752889085 1107 3 0.10084677217412263 1107 4 -0.19529064547212727 1107 5 0.41775804517776294 1107 6 0.3131834618342637 1107 7 0.05723371898454843 1107 8 0.008987482596459984 1107 9 0.1556467009149074 1107 10-11 -0.02823842309123137 1107 12-13 0.11106612972380248 1107 14-15 0.04696934027137445 1107 16-17 -0.03976281633439527 1107 18-19 0.14906051537276 1107 20-21 0.05037596314173953 1107 22-23 0.32025891336163426 1107 24-25 0.28603136168431575 1107 26-27 0.5447241367518174 1107 28-29 0.5451725813786297 1107 30-31 0.419131232322826 1107 32-33 0.28137862201959507 1107 34-35 0.08831113848052752 1107 36-37 0.18353869645503806 1107 38-39 0.4076520172747564 1107 40-41 0.03952200216063062 1107 42-43 -0.23239540918390134 1107 44-45 -0.05046425162748491 1107 46-47 0.2750842806897431 1107 48-49 0.232810782262046 1107 50-51 0.06564126660775571 1107 52-53 -0.5456029751005254 1107 54-55 -0.3309511103535634 1107 56-57 -0.018713844209351294 1107 58-59 0.15776544906180945 1107 60-61 -0.012109903907727926 1107 62-63 -0.2586680310914886 1107 64-65 -0.41234082682407447 1107 66-67 -0.2924376539823079 1107 68-69 -2.6515784703983662 1107 70-71 -2.7270983484767264 1107 72-73 0.7043200619514494 1107 74-75 0.7218285711474799 1107 76-77 0.3231180827840028 1107 78-79 0.05755282575411513 1107 80-81 0.028665636134572736 1107 82-83 0.023329141863879244 1107 84-85 0.016307992772048863 1107 86-87 0.058002175843945736 1107 88-89 -0.11262050106935106 1107 90-91 -0.22269020448407417 1107 92-93 -0.14000976979198398 1107 94-95 0.2498226769708971 1107 96-97 0.1774917146033026 1107 98-99 0.20667929160328313 1107 100-101 0.5467890728673872 1108 1 -0.037485898666506756 1108 2 -0.030451968651497907 1108 3 -0.1404685358024338 1108 4 -0.13438120347453975 1108 5 0.08112374452206694 1108 6 -0.02142171996339215 1108 7 0.19530560234029082 1108 8 0.19236474675755488 1108 9 0.045850583570000936 1108 10-11 0.14879085404441383 1108 12-13 0.005595739242401976 1108 14-15 0.1688942702599192 1108 16-17 0.17518238795977936 1108 18-19 0.33440833567915007 1108 20-21 0.20104674361913766 1108 22-23 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1.200923663448311 2310 96-97 1.0836119814944034 2310 98-99 1.1670381136569965 2310 100-101 1.164868456379697 2311 1 -0.3519753517185862 2311 2 -0.3305328596974846 2311 3 -0.2784450151752189 2311 4 -0.20767595800132455 2311 5 0.1626158732010552 2311 6 0.19561517879835577 2311 7 -3.0377215894959377 2311 8 -0.9427693457071342 2311 9 -3.448801896051677 2311 10-11 -1.619298250553932 2311 12-13 -0.9710335930953491 2311 14-15 -1.9339064266475532 2311 16-17 0.12110859043629318 2311 18-19 0.288140524093059 2311 20-21 -0.8750717306512854 2311 22-23 -0.19513490145663326 2311 24-25 -0.23368647443640356 2311 26-27 0.4163003303423949 2311 28-29 0.2693533406673012 2311 30-31 0.27758462545248364 2311 32-33 0.49364496478953157 2311 34-35 -0.33747228672735474 2311 36-37 -0.08787118862391452 2311 38-39 0.29481200357142967 2311 40-41 0.1647006125190913 2311 42-43 0.09685308812215254 2311 44-45 0.11906142653128526 2311 46-47 0.4022592160661205 2311 48-49 0.7332362081551018 2311 50-51 0.2166269715502942 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1.5628770235943144 2312 76-77 1.452547163146086 2312 78-79 0.4757621456608234 2312 80-81 0.4077426629746377 2312 82-83 0.44831950671485643 2312 84-85 0.31375245478485425 2312 86-87 0.588487540299635 2312 88-89 0.48644797766414527 2312 90-91 0.681461680052255 2312 92-93 0.6072114779351132 2312 94-95 0.44819804261245366 2312 96-97 0.5469107398226889 2312 98-99 0.8582148625970341 2312 100-101 1.0588200387540176 2313 1 -0.2589632025774051 2313 2 -0.1688096366839389 2313 3 0.08623449885913459 2313 4 0.08442291087173714 2313 5 0.054382386257891824 2313 6 -0.0012497841956431444 2313 7 -2.6900145653373 2313 8 -3.4472254272599905 2313 9 -0.8376149109937217 2313 10-11 -0.09077779251735052 2313 12-13 -0.046817701460092565 2313 14-15 -0.7430797264660214 2313 16-17 -0.2418895941748005 2313 18-19 0.12714624955039966 2313 20-21 -2.461836847255107 2313 22-23 -0.6378810262995316 2313 24-25 0.03570397382501511 2313 26-27 -1.700182702753544 2313 28-29 -1.661156364681112 2313 30-31 -0.651398058530205 2313 32-33 -1.066858456520336 2313 34-35 -1.349091473991706 2313 36-37 -1.660724142122028 2313 38-39 -1.7677532806067617 2313 40-41 -0.48120289257796145 2313 42-43 0.07081133417717211 2313 44-45 0.5181048562198747 2313 46-47 0.3141304630986568 2313 48-49 -1.724069335763346 2313 50-51 -0.12360134851673621 2313 52-53 0.1684672433790695 2313 54-55 0.06661378703728005 2313 56-57 0.04799868438528776 2313 58-59 0.3932899327007071 2313 60-61 0.23448774884564472 2313 62-63 0.5535190855746279 2313 64-65 0.31905608329759616 2313 66-67 1.0333878607211346 2313 68-69 1.7919533400979368 2313 70-71 4.127756937801163 2313 72-73 2.984717403064735 2313 74-75 1.9879534592554258 2313 76-77 2.035920191327584 2313 78-79 1.6999872652992494 2313 80-81 1.6459865259664817 2313 82-83 1.5890885159314472 2313 84-85 1.6388588000524926 2313 86-87 1.6111136037523117 2313 88-89 1.2676342812798858 2313 90-91 1.8027755979891538 2313 92-93 1.8120341250460648 2313 94-95 2.0701406161679294 2313 96-97 2.2837394353207365 2313 98-99 2.5325130946069585 2313 100-101 2.430156111955011 2314 1 -0.17985413959737429 2314 2 -0.15719952636414902 2314 3 -0.11359653032673123 2314 4 0.021414951089582246 2314 5 -0.2737477631625822 2314 6 -2.765596942413765 2314 7 -0.9225700743444207 2314 8 0.022079139141354176 2314 9 -0.1991049263547069 2314 10-11 -0.3126315838872671 2314 12-13 -2.404366926428679 2314 14-15 -0.6399670327081566 2314 16-17 -1.5982853489576527 2314 18-19 -0.34095038499784636 2314 20-21 -0.5471929427724973 2314 22-23 -0.31452884085057775 2314 24-25 -0.25914101989094007 2314 26-27 -1.9406693666272972 2314 28-29 -0.8215557502417852 2314 30-31 -0.42180931394145915 2314 32-33 -0.10211261096804236 2314 34-35 -0.20716925642432926 2314 36-37 -0.5418105825633077 2314 38-39 -0.4930667843073593 2314 40-41 -0.4613599935415138 2314 42-43 -0.4267832755142109 2314 44-45 -3.6863931189232595 2314 46-47 -3.5225892687823652 2314 48-49 -3.3013092463903533 2314 50-51 -3.463979089055769 2314 52-53 -1.3333453526270773 2314 54-55 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-0.46827634126046647 2315 78-79 -0.6153093470587834 2315 80-81 -1.2486353126456322 2315 82-83 -1.7462438494495274 2315 84-85 -1.5118175231823834 2315 86-87 -0.9284815779109223 2315 88-89 -0.8485465440812341 2315 90-91 -0.731078583622427 2315 92-93 -1.6738282229462023 2315 94-95 -1.0118316537186125 2315 96-97 -1.1951832107472917 2315 98-99 -2.7505936020547495 2315 100-101 -4.098948455594101 2316 1 -0.5213912725852872 2316 2 -0.3136306772512576 2316 3 -0.9894859949208588 2316 4 -0.18814698956044396 2316 5 -0.20627877274021955 2316 6 -0.6557351116711061 2316 7 -0.6148969702464484 2316 8 -0.6341478980701396 2316 9 -4.926963302865932 2316 10-11 -1.2960230111128936 2316 12-13 -0.6838040437155328 2316 14-15 -0.3205581773148509 2316 16-17 -0.2174602586766028 2316 18-19 -0.2133542160545332 2316 20-21 -4.005221675697605 2316 22-23 -1.4076125274575801 2316 24-25 -0.535787275190053 2316 26-27 -0.7826186522294378 2316 28-29 -0.48424141518133723 2316 30-31 -0.4163869556434534 2316 32-33 -1.9388499323685693 2316 34-35 -1.1756446842779908 2316 36-37 -0.8956785367162396 2316 38-39 -5.432165543924256 2316 40-41 -5.017335028868878 2316 42-43 -3.5085504289134875 2316 44-45 -1.8943315707995332 2316 46-47 -2.2435030700070477 2316 48-49 -2.5511388899764746 2316 50-51 -0.8848803294388716 2316 52-53 -0.7021363716253504 2316 54-55 -0.01943060063254265 2316 56-57 -2.976976841509515 2316 58-59 -0.454552764465074 2316 60-61 -0.2748362883072417 2316 62-63 0.1465893322258509 2316 64-65 0.2925673413859897 2316 66-67 0.20299937907074295 2316 68-69 2.716576080720177 2316 70-71 3.316016530456544 2316 72-73 2.160381785481583 2316 74-75 0.963640524989291 2316 76-77 -1.5346458171249395 2316 78-79 0.05860453804822896 2316 80-81 0.26915096807627137 2316 82-83 0.5424742994609701 2316 84-85 0.7125169027721014 2316 86-87 0.7608981122406462 2316 88-89 0.5396221755517523 2316 90-91 0.3209252362499413 2316 92-93 0.6502035813627707 2316 94-95 0.7537978831863867 2316 96-97 0.9247878821104507 2316 98-99 1.0957129943228239 2316 100-101 1.3811004542582026 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 32.0 4 75.0 5 308.0 6 809.0 7 1685.0 8 2547.0 9 3802.0 10 5652.0 11 8192.0 12 10228.0 13 11650.0 14 12356.0 15 14904.0 16 17037.0 17 18781.0 18 15656.0 19 12414.0 20 11845.0 21 12006.0 22 12842.0 23 14266.0 24 16663.0 25 19957.0 26 24485.0 27 29792.0 28 35419.0 29 41465.0 30 47734.0 31 55143.0 32 63744.0 33 73327.0 34 85075.0 35 89585.0 36 74864.0 37 56673.0 38 22222.0 39 1194.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.02392500835801 20.110617865261297 14.240643626327138 16.624813500053556 2 15.745012333924786 25.127234171463193 39.75148223482062 19.376271259791405 3 19.574271070639675 34.56435153033519 30.090065531706834 15.771311867318305 4 11.197557975345859 19.84773079688488 36.79604459575353 32.15866663201573 5 10.341720392886504 41.96984120115962 33.13811604863485 14.550322357319025 6 23.075050841590585 37.32248712734196 22.487776383540307 17.11468564752715 7 23.514019298169703 37.41335294881225 23.511206784648003 15.56142096837004 8 25.26372722945783 32.36088875427286 22.79185236467483 19.58353165159448 9 27.180347020899138 18.68839513651508 23.73772229674181 30.39353554584397 10-11 22.388703168781714 29.261465061179447 28.403275886317658 19.94655588372118 12-13 26.258978408550043 27.92966552723811 29.396715849595427 16.414640214616416 14-15 22.604171173899875 27.839016052961796 31.85690796590368 17.699904807234653 16-17 17.13309520615813 31.157916827124865 32.64374169764051 19.065246269076496 18-19 16.6657291611453 29.172936234840808 37.44568327385582 16.715651330158074 20-21 18.877374410453896 27.62780710484185 36.767772921985205 16.727045562719052 22-23 18.077686118484962 27.96293531853948 36.110317836350305 17.849060726625257 24-25 17.626880469405407 28.389658844865906 37.24758722787181 16.73587345785688 26-27 18.259214445624185 27.494727686851373 37.70762691008103 16.538430957443413 28-29 16.874993239077053 28.025756412059323 38.42919420615947 16.670056142704155 30-31 16.792878279216758 29.4648270654813 37.54274887648137 16.19954577882057 32-33 16.83265994904996 29.339488444293 36.71480417768691 17.113047428970127 34-35 17.41239874688452 28.698334948767656 36.68159140473553 17.207674899612297 36-37 18.151174347326187 28.50263408043202 36.81471144206431 16.53148013017749 38-39 18.32799969248777 28.321226843868786 36.89069401937435 16.460079444269095 40-41 17.139866162117805 29.061213266437846 37.38718964805173 16.411730923392618 42-43 16.788496994118148 30.151598644132388 35.996206515396416 17.063697846353048 44-45 17.0416224157175 30.467658104446315 34.8919952062825 17.598724273553685 46-47 17.29976031646022 30.163643016591646 33.89091692486235 18.64567974208579 48-49 17.91503070572838 30.21512953996075 33.72141736732884 18.148422386982027 50-51 18.502841907046555 30.802526837226196 33.50419236092355 17.1904388948037 52-53 17.734904549943852 31.129463017331187 33.719535129138315 17.41609730358665 54-55 16.877519999004313 31.00710131728543 33.970790603944764 18.144588079765494 56-57 17.954097636929642 31.6959701529682 32.52373187445289 17.82620033564926 58-59 17.7893728901584 30.77306760148879 32.87025015147581 18.567309356877 60-61 17.819897470961195 30.567707085235853 33.40610727737637 18.20628816642658 62-63 19.055757963878047 30.172788677057284 33.110618071361195 17.660835287703478 64-65 18.425583404236676 30.665240125684175 33.480963737335436 17.428212732743713 66-67 17.49060880916721 31.025461163668076 33.4972605077066 17.986669519458115 68-69 18.212440256011433 31.487913959942667 32.55361165209283 17.746034131953067 70-71 18.0150411701306 31.431387479218575 32.14395582338079 18.409615527270027 72-73 17.957033075402975 31.18838830341815 31.594252768133508 19.26032585304537 74-75 17.49368211999372 31.767437078244303 32.120318410329396 18.618562391432576 76-77 17.48170962326863 31.14273267060633 32.922461373737065 18.453096332387975 78-79 17.118225801565696 31.14367813604871 33.15549011377061 18.582605948614976 80-81 16.83258231823244 32.345844482270145 32.43222338872509 18.38934981077232 82-83 16.85116924350067 32.28377716898929 31.641754717134212 19.223298870375828 84-85 16.955778633551123 31.319014754878626 31.76631257842586 19.95889403314439 86-87 17.473341464535967 31.30516619835627 32.12064829663925 19.10084404046851 88-89 17.927653107032945 30.502036378977433 32.18544300031911 19.38486751367051 90-91 16.807244034521776 31.00842490604737 32.896185858533414 19.288145200897443 92-93 16.53459959065517 31.96064707638652 32.12020934569734 19.38454398726098 94-95 16.235708003518027 31.963087747784318 31.78843109397199 20.01277315472566 96-97 16.36692992712499 31.271273697634445 31.268622516749783 21.093173858490786 98-99 16.677701937339137 31.091929214148085 31.2684935449076 20.96187530360518 100-101 16.926078668533236 31.019739617930313 31.924390966901573 20.129790746634875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15352.0 1 10577.5 2 10464.5 3 13554.5 4 10066.0 5 7878.5 6 8576.0 7 11449.5 8 13496.5 9 13256.0 10 10983.5 11 8150.0 12 6851.5 13 6203.0 14 5819.5 15 5480.0 16 5029.0 17 4829.0 18 4783.5 19 4813.0 20 4960.5 21 5265.5 22 5721.5 23 6199.0 24 6617.0 25 7269.5 26 8492.5 27 9638.0 28 10515.0 29 11614.0 30 12840.5 31 14101.0 32 15710.0 33 17470.0 34 19209.5 35 20647.0 36 21963.5 37 23193.0 38 22994.0 39 21796.0 40 21089.0 41 21224.5 42 21654.0 43 22276.0 44 23319.0 45 24155.5 46 25607.5 47 30348.5 48 42111.5 49 41851.0 50 29790.0 51 23417.5 52 19387.0 53 17542.0 54 16105.0 55 14713.0 56 13379.5 57 12072.0 58 11283.5 59 10631.0 60 9803.5 61 8736.0 62 7290.0 63 5338.0 64 3673.5 65 2697.5 66 1976.5 67 1493.5 68 1092.0 69 832.5 70 704.5 71 567.5 72 513.0 73 467.5 74 354.0 75 280.0 76 195.0 77 114.0 78 73.0 79 50.0 80 34.0 81 19.0 82 10.5 83 6.5 84 4.0 85 3.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01914672666695513 2 0.01730777551815153 3 2.1634719397689412E-4 4 0.10211587555709403 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011899095668729176 12-13 0.0 14-15 0.0 16-17 4.3269438795378825E-4 18-19 1.0817359698844706E-4 20-21 0.0 22-23 2.1634719397689412E-4 24-25 0.0039483362900783175 26-27 0.003840162693089871 28-29 0.0010817359698844706 30-31 5.408679849422353E-5 32-33 5.408679849422353E-4 34-35 0.0025961663277227296 36-37 0.08415905845701181 38-39 0.09730215049110813 40-41 0.07685734066029164 42-43 0.08123837133832375 44-45 0.0790748993985548 46-47 0.07810133702565877 48-49 0.07258448357924797 50-51 0.07317943836268444 52-53 0.057061572411405824 54-55 0.04938124702522608 56-57 0.028233308813984684 58-59 0.021634719397689413 60-61 0.012602224049154082 62-63 0.001730777551815153 64-65 0.0064363290208126 66-67 0.14792739388170137 68-69 0.6875513824585695 70-71 0.8389944182423954 72-73 0.34577690277357104 74-75 0.05138245856951235 76-77 0.18248885811951018 78-79 0.013413526026567434 80-81 0.0026502531262169527 82-83 0.0018930379472978235 84-85 0.0 86-87 0.0018930379472978235 88-89 5.408679849422353E-4 90-91 0.004110596685560988 92-93 0.0038942494915840944 94-95 0.06787893211025053 96-97 0.03499415862576263 98-99 0.014170741205486566 100-101 0.011466401280775388 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 924440.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.380315400632114 #Duplication Level Percentage of deduplicated Percentage of total 1 82.97876987889407 43.46454137812943 2 10.360681462069312 10.853915254973456 3 2.7702252001007457 4.3531580913616885 4 1.1455746604024482 2.40022248107409 5 0.6242871567247615 1.6350179084903427 6 0.3697500910559633 1.1620575833354279 7 0.2793503295821432 1.0242720859548233 8 0.18785151155696395 0.7871777139071409 9 0.14358538124190678 0.6768942800733976 >10 0.9875983805835828 9.941237410729826 >50 0.0921870807870512 3.3138652853607327 >100 0.05119086949545488 4.887439861009122 >500 0.003745673374454997 1.3462559310626505 >1k 0.0035375804092074976 3.6662066424817628 >5k 6.242788957424995E-4 2.1319261400416467 >10k+ 0.0010404648262374993 8.355811952014552 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18756 2.028903985115313 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18711 2.024036173250833 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15252 1.6498637012677946 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13134 1.4207520228462638 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10806 1.168923889057159 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6710 0.7258448357924798 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6671 0.7216260655099304 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6178 0.668296482194626 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3795 0.4105188005711566 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3751 0.40575916230366493 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3598 0.3892086019644325 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2943 0.3183548959369997 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2244 0.24274155164207523 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1995 0.21580632599195188 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1757 0.19006100990870148 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1662 0.17978451819479901 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1617 0.1749167063303189 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1530 0.165505603392324 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1457 0.15760893081216737 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1405 0.1519839037687681 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1329 0.14376271039764615 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1262 0.13651507939942017 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1188 0.12851023322227512 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1068 0.11552940158366147 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1034 0.11185149928605426 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.0817359698844706E-4 0.0 3 0.0 0.0 0.0 1.0817359698844706E-4 0.0 4 0.0 0.0 0.0 1.0817359698844706E-4 0.0 5 0.0 0.0 0.0 1.0817359698844706E-4 0.0 6 0.0 0.0 0.0 1.0817359698844706E-4 0.0 7 0.0 0.0 0.0 1.0817359698844706E-4 0.0 8 1.0817359698844706E-4 0.0 0.0 1.0817359698844706E-4 0.0 9 1.0817359698844706E-4 0.0 0.0 1.0817359698844706E-4 0.0 10-11 1.0817359698844706E-4 0.0 0.0 1.622603954826706E-4 0.0 12-13 1.0817359698844706E-4 0.0 0.0 3.245207909653412E-4 0.0 14-15 1.0817359698844706E-4 0.0 0.0 3.245207909653412E-4 0.0 16-17 2.7043399247111765E-4 0.0 0.0 4.867811864480118E-4 0.0 18-19 3.245207909653412E-4 0.0 0.0 8.113019774133529E-4 0.0 20-21 3.245207909653412E-4 0.0 0.0 0.0013521699623555883 0.0 22-23 3.786075894595647E-4 0.0 0.0 0.0033533815066418586 0.0 24-25 4.3269438795378825E-4 0.0 0.0 0.005949547834364588 0.0 26-27 4.3269438795378825E-4 0.0 0.0 0.008329366968110423 0.0 28-29 4.867811864480118E-4 0.0 0.0 0.011358227683786942 0.0 30-31 5.408679849422353E-4 0.0 0.0 0.01790273030158799 0.0 32-33 5.408679849422353E-4 0.0 0.0 0.029801825970317164 0.0 34-35 5.408679849422353E-4 0.0 0.0 0.049327160226731864 0.0 36-37 5.408679849422353E-4 0.0 0.0 0.07988620137596816 0.0 38-39 5.408679849422353E-4 0.0 0.0 0.11601618277010947 1.0817359698844706E-4 40-41 5.408679849422353E-4 0.0 0.0 0.15636493444680022 1.0817359698844706E-4 42-43 5.949547834364589E-4 0.0 0.0 0.19687594651897367 1.0817359698844706E-4 44-45 6.490415819306824E-4 0.0 0.0 0.24187616286616762 1.0817359698844706E-4 46-47 7.572151789191294E-4 0.0 0.0 0.29477305179351826 1.0817359698844706E-4 48-49 8.653887759075765E-4 0.0 0.0 0.3588659080091731 1.0817359698844706E-4 50-51 8.653887759075765E-4 0.0 0.0 0.4283674440742503 1.0817359698844706E-4 52-53 8.653887759075765E-4 0.0 0.0 0.4997079312881312 1.0817359698844706E-4 54-55 8.653887759075765E-4 0.0 0.0 0.5842996841330967 1.0817359698844706E-4 56-57 8.653887759075765E-4 0.0 0.0 0.6768421963567133 1.0817359698844706E-4 58-59 8.653887759075765E-4 0.0 0.0 0.7706827917441911 1.0817359698844706E-4 60-61 8.653887759075765E-4 0.0 0.0 0.8751243996365368 1.0817359698844706E-4 62-63 8.653887759075765E-4 0.0 0.0 0.9766453204101943 1.0817359698844706E-4 64-65 8.653887759075765E-4 0.0 0.0 1.0765977240275193 1.0817359698844706E-4 66-67 9.735623728960236E-4 0.0 0.0 1.1855285361948855 1.0817359698844706E-4 68-69 0.0010817359698844706 0.0 0.0 1.3066829648219462 1.0817359698844706E-4 70-71 0.0010817359698844706 0.0 0.0 1.465589978797975 1.0817359698844706E-4 72-73 0.0011899095668729178 0.0 0.0 1.647105274544589 1.0817359698844706E-4 74-75 0.0012980831638613648 0.0 0.0 1.8378694128337156 1.0817359698844706E-4 76-77 0.0012980831638613648 0.0 0.0 2.0396672580156636 1.0817359698844706E-4 78-79 0.0012980831638613648 0.0 0.0 2.273754921898663 1.0817359698844706E-4 80-81 0.001730777551815153 0.0 0.0 2.5302345203582712 1.0817359698844706E-4 82-83 0.001730777551815153 0.0 0.0 2.803102418761629 1.0817359698844706E-4 84-85 0.001730777551815153 0.0 0.0 3.1124789061485876 1.0817359698844706E-4 86-87 0.001730777551815153 0.0 0.0 3.4467894076413828 1.0817359698844706E-4 88-89 0.001730777551815153 0.0 0.0 3.758004846177145 1.0817359698844706E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 105 0.0 58.759373 2 TACCTGG 1150 0.0 56.951397 2 ACCTGGG 1310 0.0 48.892754 3 ATAACGC 130 0.0 47.44408 3 GTACCTG 1510 0.0 44.94995 1 TATCACG 65 2.6040598E-6 43.808765 2 TAACGCA 145 0.0 42.538376 4 CCTGGGA 1300 0.0 33.212654 4 CATTCCG 160 5.4569682E-11 32.593105 9 GATCGGT 60 0.003988162 31.605436 6 GTCGGGA 315 0.0 28.626362 2 GTACATG 11505 0.0 25.08344 1 TACATGG 11385 0.0 24.59473 2 CGGGAGT 380 0.0 23.723324 4 ATCACGC 120 1.7241764E-4 23.722042 3 AGTACTC 360 0.0 23.704077 5 ACATGGG 11495 0.0 23.443443 3 TGGGATA 810 0.0 23.411434 6 AAGTACT 450 0.0 23.196138 4 ATGGGAT 2810 0.0 23.113583 5 >>END_MODULE