FastQCFastQC Report
Fri 27 May 2016
SRR939537_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939537_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688432
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83370.4937717361433567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68360.4048726866110095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44850.2656310707212372No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35290.20901049020629792No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18000.10660778758042966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62900.042.326331
GTATCAA100550.035.2375221
TATCAAC126100.027.5132752
ATCAACG130800.027.2176933
TCAACGC132400.027.04834
CAACGCA133400.026.5507265
GTACATG79450.026.1812171
TACATGG78950.025.762742
AACGCAG135900.025.7461346
ACATGGG79950.025.2569033
GAGTACT84100.023.38294812-13
ACGCAGA148250.023.1236677
CGCAGAG150700.022.6696578
TAGCCCT9500.022.1449764
CATGGGG34550.021.8652884
GCAGAGT148250.021.421319
GTACTTT94550.021.06770914-15
AGTACTT88900.020.80181112-13
CATGGGA47350.020.801444
GTGTAGC9400.020.6463931
Produced by FastQC (version 0.11.5)