FastQCFastQC Report
Fri 27 May 2016
SRR939537_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939537_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688432
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114090.6757156936139567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110870.6566447449467908No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73160.43330142996579074No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54630.323554635306604No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29790.17643588844561106No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22870.13545111677580146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67700.043.6183431
GTATCAA107750.033.5202641
TATCAAC137100.026.2320692
ATCAACG138350.025.8519383
TCAACGC139300.025.6416264
CAACGCA142150.025.0942065
GTACATG88850.024.5397511
AACGCAG147050.024.290236
TACATGG90600.023.4826092
ACATGGG89850.023.040333
GAGTACT98550.021.96901712-13
ACGCAGA166550.021.2756237
CGCAGAG168000.021.0355998
GTGTAGG8950.020.1247431
AGTACTT102900.020.05043612-13
GTACTTT108150.019.88750814-15
TATAACG1200.004832492719.7431762
GCAGAGT174750.019.735119
CATGGGA53250.019.5716934
AGAGTAC151650.019.18011910-11
Produced by FastQC (version 0.11.5)