##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939537_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1688432 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.918407729775318 31.0 31.0 31.0 28.0 34.0 2 31.181884138656457 31.0 31.0 34.0 28.0 34.0 3 31.151633586665024 31.0 31.0 34.0 28.0 34.0 4 35.10355347446625 35.0 35.0 37.0 32.0 37.0 5 34.72731563959935 35.0 35.0 37.0 32.0 37.0 6 34.69929378263383 35.0 35.0 37.0 31.0 37.0 7 34.56582853203446 35.0 35.0 37.0 30.0 37.0 8 34.58456958882561 35.0 35.0 37.0 30.0 37.0 9 35.92000447752708 37.0 35.0 39.0 30.0 39.0 10-11 35.781371118292 37.0 35.0 39.0 30.0 39.0 12-13 35.73230488405811 37.0 35.0 39.0 30.0 39.0 14-15 36.55164495816236 38.0 35.0 40.0 30.0 41.0 16-17 36.562941533920224 38.0 35.0 40.0 30.0 41.0 18-19 36.54579722488084 38.0 35.0 40.0 30.0 41.0 20-21 36.45781322552522 38.0 35.0 40.0 30.0 41.0 22-23 36.3129584134866 38.0 34.0 40.0 30.0 41.0 24-25 36.08203439641039 38.0 34.0 40.0 29.0 41.0 26-27 35.684890182133486 38.0 34.0 40.0 27.0 41.0 28-29 35.396017725321485 38.0 34.0 40.0 27.0 41.0 30-31 35.13978738853564 37.5 33.5 40.0 27.0 41.0 32-33 34.88668273285509 37.0 33.0 40.0 26.0 41.0 34-35 34.8056883546391 37.0 33.0 40.0 25.5 41.0 36-37 34.75600261070627 37.5 33.0 40.0 25.0 41.0 38-39 34.704426947605825 38.0 33.0 40.0 25.0 41.0 40-41 34.463330770797995 37.0 33.0 40.0 24.5 41.0 42-43 34.12768325878685 37.0 33.0 40.0 24.0 41.0 44-45 33.89567421133928 37.0 33.0 40.0 23.5 41.0 46-47 33.502336191211725 37.0 32.0 40.0 22.5 41.0 48-49 33.14175104475632 37.0 31.5 40.0 20.5 41.0 50-51 32.859814312924655 37.0 31.0 39.5 19.0 41.0 52-53 32.480067008917146 36.0 31.0 39.0 17.5 40.5 54-55 32.245322583319904 36.0 31.0 39.0 16.0 40.0 56-57 31.841545883991774 35.5 30.0 39.0 13.5 40.0 58-59 31.396586300188577 35.0 29.5 39.0 10.5 40.0 60-61 31.097785993158148 35.0 29.0 38.0 8.5 40.0 62-63 30.7127929937362 35.0 28.0 38.0 8.0 40.0 64-65 30.269088716631764 34.0 28.0 38.0 7.0 40.0 66-67 28.48837856662276 33.0 25.0 36.5 3.5 39.5 68-69 24.711234743241064 29.0 13.0 35.0 2.0 38.0 70-71 24.07765340860633 28.5 10.5 34.0 2.0 38.0 72-73 25.987276064419532 31.0 20.0 35.0 2.0 38.0 74-75 26.63663772067812 31.0 22.0 35.0 2.0 37.0 76-77 26.76210264908507 31.5 24.5 34.5 2.0 36.0 78-79 27.610001172685664 32.5 26.0 35.0 2.0 37.0 80-81 27.467900395159532 33.0 26.0 35.0 2.0 36.5 82-83 27.062549454168128 32.0 25.5 35.0 2.0 36.0 84-85 26.70821300472865 32.0 25.0 35.0 2.0 36.0 86-87 26.259631421342405 32.0 24.0 34.0 2.0 35.0 88-89 25.751811147857893 31.5 22.5 34.0 2.0 35.0 90-91 25.280680832867418 31.0 20.5 34.0 2.0 35.0 92-93 24.684144519885905 31.0 19.0 34.0 2.0 35.0 94-95 23.24048110910004 30.0 6.5 34.0 2.0 35.0 96-97 22.42042824348271 29.5 2.0 34.0 2.0 35.0 98-99 22.082727939295154 30.0 2.0 34.0 2.0 35.0 100-101 20.842398746292417 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4198492090334298 1101 2 0.2423140834524915 1101 3 0.40122921055708005 1101 4 0.24405827458190288 1101 5 0.292783654174805 1101 6 0.416527954456285 1101 7 0.4661564625474597 1101 8 0.4181938651790986 1101 9 0.5400497812461325 1101 10-11 0.7380408840600055 1101 12-13 0.6527946323356133 1101 14-15 0.6412989829283688 1101 16-17 0.7726392282515491 1101 18-19 0.7845339282764954 1101 20-21 0.6817159668114243 1101 22-23 0.7121400234910666 1101 24-25 0.690161150080776 1101 26-27 0.7138799992898228 1101 28-29 0.6519902654148098 1101 30-31 0.8023179670888538 1101 32-33 0.7972366490639544 1101 34-35 0.9234551452165292 1101 36-37 0.7289639874536107 1101 38-39 0.677352322854361 1101 40-41 0.8474869572756276 1101 42-43 0.8926957688237493 1101 44-45 0.6364324791964933 1101 46-47 0.7076422510718103 1101 48-49 0.6134078384366788 1101 50-51 0.35964572656606464 1101 52-53 0.5794663846370582 1101 54-55 0.5887312245488943 1101 56-57 0.24188680214209057 1101 58-59 0.14709053822880236 1101 60-61 0.1265199038124294 1101 62-63 0.07694392498548552 1101 64-65 -0.2182844361876768 1101 66-67 -3.1610801603258913 1101 68-69 -8.249711290352472 1101 70-71 -11.436482855045181 1101 72-73 -11.223447176651636 1101 74-75 -10.156832240593165 1101 76-77 -5.069788703216961 1101 78-79 -1.9585533252674203 1101 80-81 -1.1189941803259025 1101 82-83 -0.779500035522215 1101 84-85 -0.8347307823404257 1101 86-87 -0.7374742928177156 1101 88-89 -0.7154515020308949 1101 90-91 -0.7797991499383379 1101 92-93 -1.3185191204494089 1101 94-95 -5.203697170757767 1101 96-97 -2.05175671625452 1101 98-99 -1.267378240887222 1101 100-101 -1.0416522563285788 1102 1 0.33179740126054114 1102 2 0.23052717539677303 1102 3 0.30155534658938876 1102 4 0.20903537370585212 1102 5 0.3230511939822236 1102 6 0.2682451818448257 1102 7 0.3253842915099625 1102 8 0.4033680237887296 1102 9 0.5262792378131991 1102 10-11 0.4406619056248431 1102 12-13 0.4859313326656931 1102 14-15 0.3670310051563064 1102 16-17 0.4838694537172188 1102 18-19 0.6301344134822742 1102 20-21 0.7466641553478226 1102 22-23 0.6324304580233431 1102 24-25 0.5827034487308396 1102 26-27 0.6410000987914586 1102 28-29 0.7673660230874972 1102 30-31 0.5199082757678113 1102 32-33 0.39658831376019776 1102 34-35 0.5956641970599819 1102 36-37 0.785168138029789 1102 38-39 0.8243716412780344 1102 40-41 0.6834720271100707 1102 42-43 0.8229501450870416 1102 44-45 0.4612728250901412 1102 46-47 0.4470302815964686 1102 48-49 0.5176075011054806 1102 50-51 0.4716108960743739 1102 52-53 0.561479201254123 1102 54-55 0.306684084298098 1102 56-57 0.3921920856442327 1102 58-59 0.2599898272743957 1102 60-61 0.11651216561686795 1102 62-63 0.02350842450630708 1102 64-65 -0.06871914070301699 1102 66-67 -2.9388485336193995 1102 68-69 -7.716897896507845 1102 70-71 -10.821567083273973 1102 72-73 -9.379840537796543 1102 74-75 -7.761745318143664 1102 76-77 -4.140341855024609 1102 78-79 -1.5949662717975954 1102 80-81 -0.6671029973169595 1102 82-83 -0.5689674778948053 1102 84-85 -0.7296425189317581 1102 86-87 -0.8010991145870179 1102 88-89 -0.6833874460107872 1102 90-91 -0.6661815250599794 1102 92-93 -0.7320676059652023 1102 94-95 -4.159670405704883 1102 96-97 -1.1022122117881423 1102 98-99 -0.3980548347525179 1102 100-101 -0.07934268175710102 1103 1 0.3444648640330179 1103 2 0.30345901307271106 1103 3 0.33085557645818753 1103 4 0.18398775346550877 1103 5 0.4126941324230131 1103 6 0.42684360343368155 1103 7 0.518224348646882 1103 8 0.3633115253233612 1103 9 0.6185290421632033 1103 10-11 0.6938968528527525 1103 12-13 0.5459959444364131 1103 14-15 0.6690864904407547 1103 16-17 0.7959689605588949 1103 18-19 0.7160110740516856 1103 20-21 0.6849358739713196 1103 22-23 0.8478268055134635 1103 24-25 0.8290990563484044 1103 26-27 0.8258178305872903 1103 28-29 0.9662180051134825 1103 30-31 0.9527683411408177 1103 32-33 0.7099581029443982 1103 34-35 0.7244414330503446 1103 36-37 0.888313934667238 1103 38-39 0.9344478803189915 1103 40-41 1.0197820398472714 1103 42-43 0.8583451763750816 1103 44-45 0.6717994704471053 1103 46-47 0.8508771768452021 1103 48-49 0.676083142649631 1103 50-51 0.5444736085851503 1103 52-53 0.5306499914885876 1103 54-55 0.6393611582924734 1103 56-57 0.7162956746768288 1103 58-59 0.7107040756284668 1103 60-61 0.4500111292305107 1103 62-63 0.20668200872347242 1103 64-65 0.18480937361178817 1103 66-67 -3.0030898769509555 1103 68-69 -7.468448669862511 1103 70-71 -11.161311867761842 1103 72-73 -8.905413902188315 1103 74-75 -7.458080063693071 1103 76-77 -3.9685561199539876 1103 78-79 -1.010455170885411 1103 80-81 -0.3723725694997171 1103 82-83 -0.10352760487883472 1103 84-85 -0.17874957650061774 1103 86-87 -0.04736162863612492 1103 88-89 -0.00725615681785996 1103 90-91 0.1312129841673091 1103 92-93 0.08130525272012434 1103 94-95 1.0011586837362039 1103 96-97 1.2946833684494763 1103 98-99 0.6540435621575504 1103 100-101 0.6670930749350852 1104 1 0.4003917441759697 1104 2 0.14259693419558772 1104 3 0.2475981188588534 1104 4 0.09426862045465612 1104 5 0.38298021594732035 1104 6 0.48232288578103066 1104 7 0.5308735677857896 1104 8 0.5398249669387098 1104 9 0.7561059942155097 1104 10-11 0.7527805645355059 1104 12-13 0.7550900120209221 1104 14-15 0.820806704414089 1104 16-17 0.7614488278209137 1104 18-19 0.8521468264716106 1104 20-21 0.8107020682629056 1104 22-23 0.639032098765334 1104 24-25 0.938810849056658 1104 26-27 1.0625743456706829 1104 28-29 1.0992933735030377 1104 30-31 0.9906237427023115 1104 32-33 1.0322906472705142 1104 34-35 0.9456250342520107 1104 36-37 1.037053594166565 1104 38-39 1.0330049987431238 1104 40-41 1.0883285168409316 1104 42-43 1.2866214579344302 1104 44-45 1.161894957140042 1104 46-47 1.0919510149172211 1104 48-49 0.9427615578170432 1104 50-51 0.9323732050944358 1104 52-53 0.8776838841559993 1104 54-55 0.8414786749305279 1104 56-57 0.9575579155225995 1104 58-59 1.0256636826260568 1104 60-61 0.840608989848608 1104 62-63 0.6513825939142883 1104 64-65 0.4929992836077268 1104 66-67 -2.7014070799442145 1104 68-69 -7.280059452074177 1104 70-71 -8.584220992355448 1104 72-73 -8.098838155863554 1104 74-75 -7.267677032379048 1104 76-77 -3.9568034771061527 1104 78-79 -0.560406758527094 1104 80-81 0.12618836467679628 1104 82-83 0.18373993233165464 1104 84-85 0.40793339748674384 1104 86-87 0.5481093745327072 1104 88-89 0.5695777828937523 1104 90-91 0.33069160679115583 1104 92-93 0.3280461106669428 1104 94-95 1.3022117560821158 1104 96-97 1.5438557643930295 1104 98-99 0.9997624009538093 1104 100-101 0.9583240994118825 1105 1 0.48077017237206476 1105 2 0.3155513384865003 1105 3 0.45007476866252105 1105 4 0.2799345573670635 1105 5 0.44233658962241407 1105 6 0.44172338331189565 1105 7 0.5798854367855597 1105 8 0.4960823010748072 1105 9 0.7448366873832981 1105 10-11 0.8378651405496953 1105 12-13 0.7952174580115212 1105 14-15 0.8578054039771885 1105 16-17 0.9447203577759353 1105 18-19 0.8573309827271984 1105 20-21 1.0556593040990947 1105 22-23 1.1820369862495 1105 24-25 1.062284369981633 1105 26-27 1.0258260251812388 1105 28-29 1.0564585797794166 1105 30-31 1.1722069002985478 1105 32-33 1.0761149211050665 1105 34-35 1.1756158708805202 1105 36-37 1.130317484451595 1105 38-39 1.0415854750479738 1105 40-41 1.2194841650648058 1105 42-43 1.195413771072019 1105 44-45 0.9393826908139573 1105 46-47 0.906828028927869 1105 48-49 0.8617634645601626 1105 50-51 1.0168636433385032 1105 52-53 1.1601643855384367 1105 54-55 1.2926767116834839 1105 56-57 1.4103602036143457 1105 58-59 1.2562845010442238 1105 60-61 1.1793317999774153 1105 62-63 1.5570224850542864 1105 64-65 1.430163659255168 1105 66-67 1.8882732019828872 1105 68-69 -4.933499631447702 1105 70-71 -5.491995917307854 1105 72-73 -1.6825819539223126 1105 74-75 1.102525291900605 1105 76-77 1.2233389096241005 1105 78-79 1.291537071910323 1105 80-81 1.332992485442837 1105 82-83 1.2933672127255313 1105 84-85 1.1465219049682212 1105 86-87 1.375076668915007 1105 88-89 1.430908910739717 1105 90-91 1.3620306547105088 1105 92-93 1.7196077671420902 1105 94-95 2.5503747170150426 1105 96-97 2.4947215495701656 1105 98-99 2.216153672683749 1105 100-101 2.155017164559645 1106 1 0.14541418313704924 1106 2 0.25893439753978953 1106 3 0.16046780698987106 1106 4 0.07782515719052441 1106 5 0.17975552620838187 1106 6 0.2551277179748652 1106 7 0.38900689637429053 1106 8 0.38424712346011347 1106 9 0.40181140131041815 1106 10-11 0.5659823299274152 1106 12-13 0.38587038965904696 1106 14-15 0.6210315257698085 1106 16-17 0.5485797415161571 1106 18-19 0.3542443218015663 1106 20-21 0.40111743566082225 1106 22-23 0.5800768063445005 1106 24-25 0.5858478621563208 1106 26-27 0.6735865624819937 1106 28-29 0.8776984606618683 1106 30-31 0.6568319311503359 1106 32-33 0.6422030920169775 1106 34-35 0.8643531084786389 1106 36-37 0.6811131372862462 1106 38-39 0.8306922066120848 1106 40-41 0.7738712895486799 1106 42-43 0.7418955623188381 1106 44-45 0.6060163051994607 1106 46-47 0.37311429796840656 1106 48-49 0.4649524618150309 1106 50-51 0.4115217570088845 1106 52-53 0.6091131613286507 1106 54-55 0.5730751551365927 1106 56-57 0.3482551469386692 1106 58-59 0.4648516253772037 1106 60-61 0.5956674049365489 1106 62-63 0.7741028171865914 1106 64-65 0.6896893177051098 1106 66-67 1.211193225521857 1106 68-69 -5.531693737435589 1106 70-71 -6.628852165751795 1106 72-73 -2.71912556191651 1106 74-75 0.0606573925275562 1106 76-77 0.40848831312024103 1106 78-79 0.17265556110492142 1106 80-81 0.4165679626620893 1106 82-83 0.47650276228617017 1106 84-85 0.4899682034540369 1106 86-87 0.5482352347190478 1106 88-89 0.5541639658525987 1106 90-91 0.35752494618636277 1106 92-93 0.29504025803744227 1106 94-95 1.2783485600864033 1106 96-97 1.498310730911868 1106 98-99 1.4339190762143836 1106 100-101 1.3893451290528738 1107 1 0.0048557617646203255 1107 2 0.05682588834741509 1107 3 -0.05122989308251391 1107 4 0.06501770086866543 1107 5 0.1476243596079172 1107 6 0.21303005997854285 1107 7 0.05720749402052405 1107 8 0.19770011522067676 1107 9 0.2607677210940551 1107 10-11 0.1804027943973594 1107 12-13 0.19689418062957742 1107 14-15 0.13560908344793177 1107 16-17 0.11695431816033874 1107 18-19 0.30130958537906594 1107 20-21 0.2390686634099879 1107 22-23 0.25265719031627754 1107 24-25 0.4655840839746972 1107 26-27 0.4178515531186875 1107 28-29 0.32707458609283435 1107 30-31 0.2060852885851432 1107 32-33 0.3366880823491698 1107 34-35 0.45808017598442774 1107 36-37 0.4253270553292623 1107 38-39 0.2693912533954119 1107 40-41 0.20531996574428035 1107 42-43 0.26656667004048984 1107 44-45 0.2749186319782453 1107 46-47 0.15191875637054153 1107 48-49 0.4665960581560782 1107 50-51 0.4438114008986531 1107 52-53 0.5411345702955543 1107 54-55 0.31858706446267604 1107 56-57 0.3035855893878825 1107 58-59 0.42170718682278974 1107 60-61 0.24872933841484013 1107 62-63 0.5543998686785621 1107 64-65 0.36214984025109587 1107 66-67 1.1284161774624124 1107 68-69 -5.477242740135935 1107 70-71 -6.250361340133562 1107 72-73 -2.431004438208724 1107 74-75 0.08484379503772743 1107 76-77 0.074140540591209 1107 78-79 0.1378909033397413 1107 80-81 0.14650785201864736 1107 82-83 0.21148513601298546 1107 84-85 0.19892763742021558 1107 86-87 0.2999760627594199 1107 88-89 0.1513098521558689 1107 90-91 0.0043798294321995 1107 92-93 0.2498707703827563 1107 94-95 0.9828181732772521 1107 96-97 1.203554322270218 1107 98-99 0.7490301971678441 1107 100-101 0.7777145569152566 1108 1 0.10182089766182045 1108 2 0.028708688569288654 1108 3 0.1651589508668252 1108 4 -0.09000708650306422 1108 5 0.13225272237023944 1108 6 0.34317936200313426 1108 7 0.2490383505426621 1108 8 0.17161299540926933 1108 9 0.37551707396453793 1108 10-11 0.36259113219896477 1108 12-13 0.3307474324554107 1108 14-15 0.4671511584225101 1108 16-17 0.19244876555795543 1108 18-19 0.3794659307151065 1108 20-21 0.4869210023209618 1108 22-23 0.40559281826303106 1108 24-25 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0.82205298209362 2312 94-95 1.681371818194517 2312 96-97 1.7159461017821798 2312 98-99 1.5583341708021763 2312 100-101 1.246120577987302 2313 1 -0.37784231925966694 2313 2 -0.21563732916326117 2313 3 -0.11036661838835826 2313 4 -0.040014249420877945 2313 5 -0.2229429905840945 2313 6 -0.11459563107008819 2313 7 -3.7458212720109216 2313 8 -1.1231485502696827 2313 9 -0.871648457130398 2313 10-11 -0.12227464574113611 2313 12-13 -1.2544822374521516 2313 14-15 -0.21726996761965722 2313 16-17 0.018737972693799065 2313 18-19 0.22346328751972067 2313 20-21 0.17100720292044969 2313 22-23 -1.1585213964292862 2313 24-25 -2.741741472144419 2313 26-27 -2.0508393173039465 2313 28-29 -0.3598926724489502 2313 30-31 -0.059718403665911524 2313 32-33 -2.4633297385294597 2313 34-35 -2.506528342395555 2313 36-37 -0.9032480381324319 2313 38-39 -0.43058250487122507 2313 40-41 -0.5493349065035176 2313 42-43 -0.36724129922143334 2313 44-45 -0.09443807347005162 2313 46-47 -0.11569404881200995 2313 48-49 -0.1823864871745613 2313 50-51 -0.060588201950338316 2313 52-53 0.3289098392243659 2313 54-55 0.3390405372819174 2313 56-57 0.4975190489687549 2313 58-59 0.2227876020688484 2313 60-61 0.9080767809229222 2313 62-63 0.416788806417113 2313 64-65 0.5739406035526216 2313 66-67 1.618755694575352 2313 68-69 4.756402099544701 2313 70-71 4.701507600033331 2313 72-73 2.9860979260931195 2313 74-75 1.9523173623663759 2313 76-77 1.1929895468324325 2313 78-79 0.9076314430969497 2313 80-81 1.1150900346650268 2313 82-83 0.8022629292398769 2313 84-85 0.9555849105907832 2313 86-87 1.0747965320947799 2313 88-89 0.8601920913982255 2313 90-91 1.185240989120981 2313 92-93 1.3243708555704714 2313 94-95 2.0194283288280666 2313 96-97 2.2903186969630056 2313 98-99 2.174276115501222 2313 100-101 1.7793652402774107 2314 1 -0.17356132308677985 2314 2 -0.2678596138179792 2314 3 -0.15405063504534766 2314 4 -0.14747742985702672 2314 5 -1.4131716808127877 2314 6 -2.789896087451627 2314 7 -0.829928456118104 2314 8 -0.18323982657717153 2314 9 0.11997809665831483 2314 10-11 0.31467446553229905 2314 12-13 0.17286631692343235 2314 14-15 -2.4977001899417672 2314 16-17 -2.537575825511894 2314 18-19 -0.20472716904911437 2314 20-21 0.005041028846164863 2314 22-23 0.14213726639153634 2314 24-25 -2.1843494647524153 2314 26-27 -1.296183413999394 2314 28-29 -0.21762649775033793 2314 30-31 0.13274256041667343 2314 32-33 0.08868964051694661 2314 34-35 -0.24431436126265993 2314 36-37 0.2514481341313086 2314 38-39 -1.200673593881234 2314 40-41 -3.7410572941718527 2314 42-43 -5.173116196988222 2314 44-45 -2.245412020389942 2314 46-47 -3.3280759733560927 2314 48-49 -1.1725998814420109 2314 50-51 -1.0773269781485766 2314 52-53 -2.4982148946571243 2314 54-55 -1.2028182653876947 2314 56-57 -3.6320723927848455 2314 58-59 -4.877309792623141 2314 60-61 -2.528249878376709 2314 62-63 -1.511903607410435 2314 64-65 -0.4342480424738646 2314 66-67 -0.11484360118610937 2314 68-69 2.0169553763141934 2314 70-71 1.745816565963917 2314 72-73 -0.4585450536849933 2314 74-75 0.09089315229351769 2314 76-77 0.14274231415276617 2314 78-79 0.6476225776826787 2314 80-81 0.8077083286346713 2314 82-83 0.6122424513815616 2314 84-85 0.477124745644133 2314 86-87 0.09646560241250057 2314 88-89 0.25063617373420044 2314 90-91 1.0484473347597572 2314 92-93 1.1178332368706911 2314 94-95 1.0549876927658097 2314 96-97 1.216440140652015 2314 98-99 0.8055332562178243 2314 100-101 0.6223400237927343 2315 1 -0.38911986015694566 2315 2 -0.3046664116712883 2315 3 -0.2816843062836689 2315 4 -0.16762253023468787 2315 5 -1.0263946981055696 2315 6 -0.37664822420376254 2315 7 -0.17505864892272172 2315 8 -0.07824082041537395 2315 9 0.10982107022222465 2315 10-11 -1.7597441391188653 2315 12-13 -1.389017503390619 2315 14-15 -0.20068915833771683 2315 16-17 -2.077647381862519 2315 18-19 -3.4070279044893823 2315 20-21 -2.303016514385803 2315 22-23 -1.8697241925629484 2315 24-25 -1.162035312496407 2315 26-27 -0.5994531416877251 2315 28-29 -0.692139517030796 2315 30-31 -2.866867358088598 2315 32-33 -2.402442838115526 2315 34-35 -1.4938323497341344 2315 36-37 -4.656979613831325 2315 38-39 -2.346300705787421 2315 40-41 -1.5649615875528866 2315 42-43 -1.7732851494827528 2315 44-45 -1.4374662839558354 2315 46-47 -1.3535405927160653 2315 48-49 -4.418868018989215 2315 50-51 -3.9492560181008685 2315 52-53 -2.586286351623933 2315 54-55 -4.534104292022562 2315 56-57 -6.885506103776699 2315 58-59 -7.533705141460349 2315 60-61 -6.318028252457406 2315 62-63 -7.024483114466687 2315 64-65 -6.476160684095806 2315 66-67 -5.081182301245736 2315 68-69 -3.0488764662618344 2315 70-71 -0.2057487292060287 2315 72-73 -1.8177604184236174 2315 74-75 -3.5411283146116688 2315 76-77 -5.702293464022542 2315 78-79 -4.624517652887786 2315 80-81 -4.2908058832516325 2315 82-83 -7.531543575253298 2315 84-85 -5.548953568806272 2315 86-87 -5.881304721578751 2315 88-89 -4.491738602652202 2315 90-91 -4.019098878716687 2315 92-93 -5.077903197237834 2315 94-95 -3.0929177932210727 2315 96-97 -2.161899155710536 2315 98-99 -3.727203774193626 2315 100-101 -2.8029307971176216 2316 1 -0.38365272601983236 2316 2 -0.3541102395479996 2316 3 -0.7619450303583974 2316 4 -0.2793572155329116 2316 5 -0.13684930470732581 2316 6 -2.216669916177729 2316 7 -0.29216588126225673 2316 8 -0.3275934242930276 2316 9 0.017966490525239465 2316 10-11 0.0243997005286829 2316 12-13 -0.5811207420527822 2316 14-15 -0.8196702661417774 2316 16-17 -1.1067760034973233 2316 18-19 -0.21208390005009647 2316 20-21 -4.006756067766553 2316 22-23 -4.319208377600532 2316 24-25 -2.5204100539583365 2316 26-27 -1.9877773105824446 2316 28-29 -1.2133585555214381 2316 30-31 -0.21718327898855705 2316 32-33 -2.2598633008776403 2316 34-35 -2.6651043569984196 2316 36-37 -4.626026512215358 2316 38-39 -2.381213853814117 2316 40-41 -1.8316351906065762 2316 42-43 -2.9360043813514594 2316 44-45 -0.9101264093993393 2316 46-47 -1.0121536936594069 2316 48-49 -0.005028455519173747 2316 50-51 0.042245856825566364 2316 52-53 -2.4272024171370568 2316 54-55 -0.8514713235852227 2316 56-57 0.23311662403106936 2316 58-59 0.26741182704424915 2316 60-61 -0.9863491423328021 2316 62-63 -0.6652528852716451 2316 64-65 0.4925026110663815 2316 66-67 1.431858106361588 2316 68-69 3.9027916297209444 2316 70-71 4.214749765808179 2316 72-73 2.5034142692287737 2316 74-75 1.3951620215298526 2316 76-77 0.1466635556011262 2316 78-79 0.6164955220436887 2316 80-81 0.24016193191257074 2316 82-83 0.2741461538492551 2316 84-85 0.6516635594454208 2316 86-87 0.36750032811100297 2316 88-89 0.5354656970669787 2316 90-91 0.4988033056146435 2316 92-93 0.8233646902329461 2316 94-95 1.5009830206483379 2316 96-97 1.5671298341833548 2316 98-99 0.6697887765470973 2316 100-101 0.7913714864250636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 31.0 4 67.0 5 147.0 6 390.0 7 996.0 8 2057.0 9 3271.0 10 5250.0 11 7428.0 12 9885.0 13 12332.0 14 15212.0 15 19055.0 16 22415.0 17 24850.0 18 22987.0 19 19437.0 20 18692.0 21 18572.0 22 19568.0 23 22208.0 24 27144.0 25 33221.0 26 42002.0 27 51410.0 28 62677.0 29 74835.0 30 87668.0 31 101359.0 32 116320.0 33 133994.0 34 155026.0 35 175130.0 36 179473.0 37 145072.0 38 55335.0 39 2916.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.70497125304723 20.578908237831048 15.597761854970809 22.118358654150903 2 16.262502702686692 21.617761612667234 38.71479472909453 23.404940955551538 3 19.39639855202934 25.555722706037315 30.67319264264122 24.374686099292127 4 13.255553081201969 18.107332720535975 36.083774768542646 32.55333942971941 5 13.24622666764588 38.191678887106825 33.76093028544895 14.801164159798343 6 31.316807546883734 36.81048452054924 16.885311342120975 14.987396590446048 7 27.621840855894703 33.998230310726164 20.254768921697764 18.125159911681372 8 26.44838524737745 35.32253593866972 18.9677167928587 19.26136202109413 9 26.140288741270005 17.022302349161826 20.365463341135445 36.47194556843272 10-11 25.080933078738145 27.270805102011806 26.46748580931894 21.180776009931108 12-13 26.155302671354253 24.92182095577435 27.3992378727719 21.5236385000995 14-15 22.8562654581292 26.05630549527609 26.485875652676565 24.601553393918145 16-17 21.503685832561658 29.323253719665875 27.21134406661358 21.961716381158887 18-19 21.172891165588435 27.006104563526968 30.7482073360187 21.0727969348659 20-21 22.188551919340522 26.046629667830768 30.919140042104164 20.845678370724546 22-23 21.734810759331737 26.181569645683094 30.82966918418983 21.25395041079534 24-25 21.654410719531494 26.39058013588939 30.902813971779736 21.05219517279938 26-27 21.347982536330573 26.63695248722252 30.864130626304657 21.150934350142247 28-29 21.080285398642907 26.729496228153053 31.05348114668886 21.13673722651518 30-31 21.258573056105153 26.504621506180715 30.876683923093257 21.360121514620875 32-33 21.086077189482594 26.647424282719705 30.71432759951359 21.552170928284113 34-35 21.800218271097442 26.668729925796715 30.488799908782227 21.04225189432362 36-37 21.648567695055057 26.421247536539845 30.509909572336202 21.4202751960689 38-39 21.37191722688789 26.773016057945803 30.638638855859135 21.21642785930717 40-41 21.552453362089388 26.56315831139024 30.706230158930175 21.1781581675902 42-43 21.447142102457775 26.70651787879453 30.22935989237564 21.61698012637206 44-45 21.585249483679164 26.97606293022413 29.943051301258944 21.49563628483776 46-47 21.711385998904348 27.21718204175333 29.410233667799474 21.661198291542853 48-49 21.767866735126816 27.342398374031944 29.17179030798766 21.71794458285358 50-51 21.70728200597928 27.490732410106972 29.023379079629386 21.77860650428436 52-53 22.150477467974927 27.539444713977108 28.68847381846644 21.621603999581524 54-55 21.619161242258993 27.460037776754437 28.89693536524147 22.023865615745095 56-57 21.86320285890934 27.83694997530775 28.533132200857164 21.766714964925747 58-59 22.024622790075416 27.325509588446295 28.67589194453223 21.973975676946054 60-61 21.886532833841898 27.473144536311406 29.037130449539333 21.603192180307364 62-63 21.834156702039635 27.483654536958014 28.993969991512618 21.688218769489733 64-65 21.67408278310932 27.43744919158161 28.79420610041594 22.09426192489313 66-67 21.372241859070428 27.567933545811897 29.166550154480287 21.893274440637388 68-69 21.62587021998779 26.97454961224658 29.754892765958463 21.644687401807168 70-71 21.18616068131552 27.101027337357685 29.935347024985415 21.777464956341376 72-73 21.736297100676587 27.310662416080074 28.883632914937873 22.06940756830547 74-75 21.444603529553792 27.7361279317166 28.636476485312606 22.182792053417003 76-77 21.569579882308755 27.726067532073646 28.49516570249256 22.20918688312504 78-79 21.54618770651706 27.799251126227848 28.382629895659367 22.271931271595722 80-81 21.339736241333878 28.08029142391952 28.305800848673552 22.27417148607305 82-83 21.59494726468108 28.001986458441912 28.14472244070238 22.258343836174628 84-85 21.48010698683749 27.83419764610005 28.073798648687067 22.611896718375394 86-87 21.519966832995248 27.854923967602947 28.22864503901565 22.39646416038616 88-89 21.455688967832 27.800685494164064 28.020921371912273 22.72270416609167 90-91 21.268693734446874 27.969667723210573 28.217955047082487 22.543683495260066 92-93 21.526256982578463 27.75923456458887 28.197282809715833 22.517225643116838 94-95 20.998720920533515 27.588518146810063 28.586461349032554 22.826299583623864 96-97 20.997492746300715 27.486132328136158 28.63470150738016 22.881673418182963 98-99 21.14018135209941 27.53847771454297 28.00872551502308 23.31261541833454 100-101 21.002471453893097 27.369434231043176 28.356860764892026 23.2712335501717 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4543.0 1 2993.5 2 1458.5 3 1594.5 4 1517.5 5 1416.5 6 2063.5 7 3472.0 8 4851.5 9 5624.5 10 5230.5 11 4252.5 12 3784.5 13 3610.0 14 3578.5 15 3586.0 16 3645.5 17 3767.5 18 3973.0 19 4319.5 20 4728.0 21 5230.0 22 6032.0 23 6983.0 24 7998.0 25 9372.5 26 11472.0 27 13714.5 28 15548.5 29 17865.0 30 20544.5 31 23808.0 32 27386.0 33 30413.0 34 33509.5 35 36502.0 36 40298.5 37 43883.5 38 45426.5 39 46244.0 40 46790.0 41 48658.5 42 51269.5 43 53422.5 44 55331.5 45 56483.0 46 57271.0 47 59397.5 48 63414.5 49 64223.0 50 61638.0 51 59147.0 52 56845.5 53 55708.5 54 54138.0 55 50045.5 56 45512.0 57 41101.0 58 36533.5 59 32067.0 60 27309.0 61 22375.0 62 18045.5 63 14245.0 64 10630.0 65 7980.0 66 6172.0 67 4825.0 68 3523.5 69 2428.5 70 1801.5 71 1398.5 72 1175.0 73 1033.5 74 827.0 75 610.5 76 413.5 77 262.0 78 167.0 79 98.0 80 60.0 81 34.5 82 20.5 83 14.5 84 6.0 85 4.0 86 3.0 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025289736276024148 2 0.017590284950770894 3 0.0 4 0.0 5 2.36906194623177E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.441991149184569E-4 18-19 0.001362210619083268 20-21 2.9613274327897125E-5 22-23 0.0 24-25 0.0 26-27 7.403318581974282E-4 28-29 0.015813488491097066 30-31 0.004471604423512466 32-33 0.008587849555090166 34-35 0.009742767253878154 36-37 0.10542325660731376 38-39 0.10788115837652923 40-41 0.12215475660257563 42-43 0.10808845129682453 44-45 0.10296535483809832 46-47 0.10447563182882107 48-49 0.10696314687236443 50-51 0.10509751058970689 52-53 0.0802815867029291 54-55 0.06816975750281919 56-57 0.03882300264387314 58-59 0.016613046897950286 60-61 0.010749618581026656 62-63 0.002546741592199153 64-65 0.006189174334530499 66-67 0.45219469898698905 68-69 0.854639097103111 70-71 0.9592627953035716 72-73 0.29947904327802366 74-75 0.16296184862641788 76-77 0.016938792915557156 78-79 0.009683540705222359 80-81 0.0019248628313133132 82-83 0.0 84-85 0.0 86-87 4.145858405905598E-4 88-89 8.883982298369138E-4 90-91 0.003879338936954523 92-93 0.0019840893799691075 94-95 0.2379426592246534 96-97 0.2439541539132165 98-99 0.016553820349294493 100-101 0.009150501767320213 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1688432.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.682943406850505 #Duplication Level Percentage of deduplicated Percentage of total 1 77.35094023052547 42.29777087092499 2 13.118730978134932 14.347416472940983 3 4.159258832747917 6.823215460967923 4 1.8337475317742795 4.010988500098589 5 0.924685163361378 2.528225322862227 6 0.5344093756924243 1.7533846588247488 7 0.36820140060489637 1.4094035446120448 8 0.25895703912947293 1.132842649241803 9 0.18891479327321398 0.9297375254348421 >10 1.0970576712865723 11.25712460764588 >50 0.10256651059903744 3.9013772941126756 >100 0.05859521799467046 5.857549064487108 >500 0.0027328158859628165 0.9423586540293148 >1k 7.651884480695887E-4 0.7000293179408378 >5k 2.1862527087702528E-4 0.7638694094412061 >10k+ 2.1862527087702528E-4 1.3447066464347268 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11409 0.6757156936139567 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11087 0.6566447449467908 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7316 0.43330142996579074 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5463 0.323554635306604 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2979 0.17643588844561106 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2287 0.13545111677580146 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.922654865579425E-5 0.0 10-11 0.0 0.0 0.0 5.922654865579425E-5 0.0 12-13 0.0 0.0 0.0 5.922654865579425E-5 0.0 14-15 0.0 0.0 0.0 5.922654865579425E-5 2.9613274327897125E-5 16-17 0.0 0.0 0.0 1.7767964596738275E-4 5.922654865579425E-5 18-19 0.0 0.0 0.0 1.7767964596738275E-4 5.922654865579425E-5 20-21 0.0 0.0 0.0 2.6651946895107415E-4 5.922654865579425E-5 22-23 0.0 0.0 0.0 4.73812389246354E-4 5.922654865579425E-5 24-25 0.0 0.0 0.0 8.291716811811196E-4 5.922654865579425E-5 26-27 0.0 0.0 0.0 0.0014510504420669592 1.7767964596738275E-4 28-29 0.0 0.0 0.0 0.0029909407071176093 1.7767964596738275E-4 30-31 0.0 0.0 0.0 0.006988732741383722 1.7767964596738275E-4 32-33 0.0 0.0 0.0 0.014836250438276459 1.7767964596738275E-4 34-35 0.0 0.0 0.0 0.0278068645938954 1.7767964596738275E-4 36-37 0.0 0.0 0.0 0.0484177035261118 1.7767964596738275E-4 38-39 0.0 0.0 0.0 0.07749793891610678 1.7767964596738275E-4 40-41 0.0 0.0 0.0 0.1081180645711524 1.7767964596738275E-4 42-43 0.0 0.0 0.0 0.1390639362438049 1.7767964596738275E-4 44-45 0.0 0.0 0.0 0.178183071630957 1.7767964596738275E-4 46-47 0.0 0.0 0.0 0.22734110701526622 2.0729292029527988E-4 48-49 0.0 0.0 0.0 0.2788089777971514 2.36906194623177E-4 50-51 0.0 0.0 0.0 0.33460038663090963 2.36906194623177E-4 52-53 0.0 0.0 0.0 0.3935308025434249 2.36906194623177E-4 54-55 0.0 0.0 0.0 0.45637017066722263 2.36906194623177E-4 56-57 0.0 0.0 0.0 0.5297222511774238 2.36906194623177E-4 58-59 0.0 0.0 0.0 0.6026893591213622 2.36906194623177E-4 60-61 0.0 0.0 0.0 0.6733762449420528 2.36906194623177E-4 62-63 0.0 0.0 0.0 0.7465802590806144 2.36906194623177E-4 64-65 0.0 0.0 0.0 0.8257957679077392 2.36906194623177E-4 66-67 0.0 0.0 0.0 0.9116446501843131 2.9613274327897125E-4 68-69 0.0 0.0 0.0 1.0154687899779202 2.9613274327897125E-4 70-71 0.0 0.0 0.0 1.1556876439205133 2.9613274327897125E-4 72-73 0.0 0.0 0.0 1.3075149013996419 3.8497256626266266E-4 74-75 0.0 2.9613274327897125E-5 0.0 1.4715428279018639 4.1458584059055975E-4 76-77 0.0 5.922654865579425E-5 0.0 1.6607716508571266 4.1458584059055975E-4 78-79 0.0 5.922654865579425E-5 0.0 1.8807094392904187 4.1458584059055975E-4 80-81 0.0 5.922654865579425E-5 0.0 2.117586020639268 4.1458584059055975E-4 82-83 0.0 5.922654865579425E-5 0.0 2.3632577444635023 4.73812389246354E-4 84-85 0.0 5.922654865579425E-5 0.0 2.6008154311218927 4.73812389246354E-4 86-87 5.922654865579425E-5 5.922654865579425E-5 0.0 2.857325613350138 4.73812389246354E-4 88-89 5.922654865579425E-5 5.922654865579425E-5 0.0 3.131811053095416 4.73812389246354E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6770 0.0 43.618343 1 GTATCAA 10775 0.0 33.520264 1 TATCAAC 13710 0.0 26.232069 2 ATCAACG 13835 0.0 25.851938 3 TCAACGC 13930 0.0 25.641626 4 CAACGCA 14215 0.0 25.094206 5 GTACATG 8885 0.0 24.539751 1 AACGCAG 14705 0.0 24.29023 6 TACATGG 9060 0.0 23.482609 2 ACATGGG 8985 0.0 23.04033 3 GAGTACT 9855 0.0 21.969017 12-13 ACGCAGA 16655 0.0 21.275623 7 CGCAGAG 16800 0.0 21.035599 8 GTGTAGG 895 0.0 20.124743 1 AGTACTT 10290 0.0 20.050436 12-13 GTACTTT 10815 0.0 19.887508 14-15 TATAACG 120 0.0048324927 19.743176 2 GCAGAGT 17475 0.0 19.73511 9 CATGGGA 5325 0.0 19.571693 4 AGAGTAC 15165 0.0 19.180119 10-11 >>END_MODULE