FastQCFastQC Report
Fri 27 May 2016
SRR939536_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939536_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666253
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81570.4895415042013428No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64410.3865559431850985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43750.2625651686748651No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33670.20207015381217616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62900.039.3613551
GTATCAA105150.038.6720121
TATCAAC128350.031.275172
ATCAACG133050.030.5616263
TCAACGC136250.030.1531284
CAACGCA137500.029.5796665
AACGCAG140350.028.9920986
GTACATG75750.026.0813431
ACGCAGA152050.025.8065837
CGCAGAG152150.025.5018548
TACATGG78750.024.7125782
GAGTACT83050.023.84158912-13
GCAGAGT148750.023.677939
GTATAGG4100.023.1784951
ACATGGG81500.022.8230843
GTACTTT92700.021.67863714-15
AGTACTT86900.021.2110612-13
AGAGTAC124900.020.78244810-11
CAGAGTA146050.019.9592810-11
ACTTTTT114800.019.9148316-17
Produced by FastQC (version 0.11.5)