FastQCFastQC Report
Fri 27 May 2016
SRR939536_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939536_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666253
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111160.6671255805690972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104780.6288360771143398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72590.43564812786533613No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54280.32576085384392406No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27300.16384066525311583No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22220.13335309823898292No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17540.1052661270527345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67550.042.008791
GTATCAA106600.034.009521
TATAGCG700.00847156727.0908155
TATCAAC139950.025.8287242
ATCAACG140450.025.6991393
TCAACGC142050.025.3816174
CAACGCA144500.024.9676065
AACGCAG148450.024.4310046
GTACATG89300.021.9995231
ACGCAGA165850.021.5248227
TACATGG89550.021.45412
AGTACTT99150.021.32180812-13
CGCAGAG167200.021.2943178
GAGTACT96250.021.2747712-13
GCAGAGT171100.020.199369
ACATGGG90200.020.1394633
AGAGTAC150000.020.05247110-11
CGATTCG958.321165E-619.98264538-39
GTACTTT103650.019.80160914-15
TACTTTT112500.018.83375414-15
Produced by FastQC (version 0.11.5)