##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939536_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1666253 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.81261729161178 31.0 30.0 31.0 28.0 34.0 2 31.06643288864296 31.0 31.0 34.0 28.0 34.0 3 31.051751444708575 31.0 31.0 34.0 27.0 34.0 4 34.986406626124605 35.0 35.0 37.0 32.0 37.0 5 34.54998730684956 35.0 35.0 37.0 30.0 37.0 6 34.62059243104139 35.0 35.0 37.0 30.0 37.0 7 34.46238296345153 35.0 35.0 37.0 30.0 37.0 8 34.37274051419563 35.0 35.0 37.0 30.0 37.0 9 35.85516485191624 37.0 35.0 39.0 30.0 39.0 10-11 35.64112172641249 37.0 35.0 39.0 30.0 39.0 12-13 35.62036107361847 37.0 35.0 39.0 30.0 39.0 14-15 36.40884967648971 38.0 35.0 40.0 30.0 41.0 16-17 36.31475472212203 38.0 34.5 40.0 30.0 41.0 18-19 36.34027575644275 38.0 34.5 40.0 30.0 41.0 20-21 36.23237167464965 38.0 34.0 40.0 29.5 41.0 22-23 36.06806439358249 38.0 34.0 40.0 29.0 41.0 24-25 35.8891125777418 38.0 34.0 40.0 29.0 41.0 26-27 35.41169438254575 38.0 34.0 40.0 27.0 41.0 28-29 35.173182583917324 38.0 33.5 40.0 27.0 41.0 30-31 34.83747336088817 37.0 33.0 40.0 26.5 40.5 32-33 34.55373643738376 37.0 33.0 40.0 25.0 41.0 34-35 34.74051569599575 37.0 33.0 40.0 26.0 41.0 36-37 34.554948738276835 37.0 33.0 40.0 25.0 41.0 38-39 34.34954445693421 37.0 33.0 40.0 25.0 41.0 40-41 34.16668987242633 37.0 33.0 40.0 24.0 41.0 42-43 33.95559197792892 37.0 33.0 40.0 24.0 41.0 44-45 33.52186942799203 37.0 32.0 40.0 23.0 41.0 46-47 33.040520857276775 37.0 31.0 39.5 20.0 41.0 48-49 32.61995087180638 36.0 31.0 39.0 18.5 40.0 50-51 32.34054492324995 36.0 31.0 39.0 17.0 40.0 52-53 32.2045901792825 36.0 30.5 39.0 16.0 40.0 54-55 31.843862546684086 35.5 30.0 39.0 14.0 40.0 56-57 31.34561288111709 35.0 29.5 39.0 11.0 40.0 58-59 30.87870119363626 35.0 28.0 38.0 8.0 40.0 60-61 30.49834358887876 34.0 28.0 38.0 8.0 40.0 62-63 30.31612216152049 34.0 28.0 38.0 7.5 40.0 64-65 29.849879940201156 34.0 28.0 38.0 4.0 40.0 66-67 28.92994461225276 33.5 27.0 37.0 2.0 39.0 68-69 25.427280851107245 31.0 15.5 35.0 2.0 38.5 70-71 24.067701603537998 29.0 10.5 35.0 2.0 38.0 72-73 25.098507849648286 30.0 16.0 35.0 2.0 37.5 74-75 25.86833031958532 30.5 20.5 35.0 2.0 37.0 76-77 25.576493485683145 30.5 21.0 34.0 2.0 36.0 78-79 26.729217891880765 31.5 24.5 35.0 2.0 36.0 80-81 26.745059723823452 32.0 25.0 35.0 2.0 36.0 82-83 26.41322596268394 32.0 24.0 34.5 2.0 36.0 84-85 26.040408629421822 32.0 23.5 34.0 2.0 35.5 86-87 25.592367125520553 31.0 21.5 34.0 2.0 35.0 88-89 25.083765490594764 31.0 20.0 34.0 2.0 35.0 90-91 24.463148453446145 31.0 18.0 34.0 2.0 35.0 92-93 23.87441552993453 30.5 13.0 34.0 2.0 35.0 94-95 22.978132972603802 30.0 4.5 34.0 2.0 35.0 96-97 22.28199799190159 29.5 2.0 34.0 2.0 35.0 98-99 21.413712083339085 29.0 2.0 34.0 2.0 35.0 100-101 20.029730479104913 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3072117161220973 1101 2 0.24971747205627537 1101 3 0.35600588420035706 1101 4 0.37922733814964005 1101 5 0.6063462548768328 1101 6 0.5015946858534335 1101 7 0.6241254214086069 1101 8 0.6455555600400942 1101 9 0.6657318733276796 1101 10-11 0.8067497061560047 1101 12-13 0.7107355306281988 1101 14-15 0.7674291234724464 1101 16-17 0.8228510241831017 1101 18-19 0.40721805394159816 1101 20-21 0.7503787156796164 1101 22-23 0.5808155904190073 1101 24-25 0.733314743258255 1101 26-27 0.7320402407460165 1101 28-29 0.6069062267917076 1101 30-31 0.43369838070022126 1101 32-33 0.946546282523876 1101 34-35 0.9619666394649684 1101 36-37 0.8710083855562374 1101 38-39 0.8882291459533249 1101 40-41 0.8262137995250143 1101 42-43 0.5432730761762414 1101 44-45 0.6610749331440786 1101 46-47 0.7148752386401256 1101 48-49 0.7391728931408679 1101 50-51 0.3609078363311369 1101 52-53 0.10514873962685556 1101 54-55 0.15138553439708602 1101 56-57 0.2633622047315569 1101 58-59 0.27422737786095297 1101 60-61 0.2626737556450962 1101 62-63 -0.1334326953116971 1101 64-65 -0.15583957549268845 1101 66-67 -0.3660519019659887 1101 68-69 -6.802406472820568 1101 70-71 -7.817786014907188 1101 72-73 -5.320266543222537 1101 74-75 -3.1878914982983915 1101 76-77 -0.7112721708444099 1101 78-79 -0.259326897300415 1101 80-81 -0.17410078925760075 1101 82-83 -0.4595716779829182 1101 84-85 -0.577265064603754 1101 86-87 -0.7242494372949615 1101 88-89 -0.5912684894795994 1101 90-91 -0.7558904300500409 1101 92-93 -0.5512139446855038 1101 94-95 -0.5459013686184839 1101 96-97 -0.7225711209229075 1101 98-99 -1.082831376729363 1101 100-101 -1.125926102080431 1102 1 0.39870997664086616 1102 2 0.33814617729109386 1102 3 0.3540991451584894 1102 4 0.34536437172501877 1102 5 0.4414612583518007 1102 6 0.39868819445297987 1102 7 0.5533920932677532 1102 8 0.6942460562016493 1102 9 0.6874501752265303 1102 10-11 0.7091233191643695 1102 12-13 0.5706165479151721 1102 14-15 0.8171085626445276 1102 16-17 0.6418431236231115 1102 18-19 0.5586224453827882 1102 20-21 0.5772379235466261 1102 22-23 0.606290827276041 1102 24-25 0.6215176254847918 1102 26-27 0.6553110945497167 1102 28-29 0.5510707016321348 1102 30-31 0.34853369915909127 1102 32-33 0.5549305920436893 1102 34-35 0.3635753600375793 1102 36-37 0.7496175345085447 1102 38-39 0.7639880470645224 1102 40-41 0.5593897856970358 1102 42-43 0.29349175952275175 1102 44-45 0.3231834500116548 1102 46-47 0.5393656265584212 1102 48-49 0.648048715949443 1102 50-51 0.3061944363343301 1102 52-53 0.19744771570948672 1102 54-55 0.038313637503787135 1102 56-57 0.33753168232799524 1102 58-59 0.26541289123758105 1102 60-61 0.24826025139924113 1102 62-63 -0.05186592898932929 1102 64-65 -0.15538111434442925 1102 66-67 -0.24559919248931195 1102 68-69 -6.977040602600642 1102 70-71 -7.5477739923159 1102 72-73 -1.56453671501378 1102 74-75 0.12844394797540204 1102 76-77 0.5590198213508941 1102 78-79 0.17294105370652701 1102 80-81 -0.01783980516257344 1102 82-83 -0.10747451467538482 1102 84-85 -0.428580609694464 1102 86-87 -0.27201202706497085 1102 88-89 -0.2992851563459773 1102 90-91 -0.7118641800600223 1102 92-93 -0.5428290859609355 1102 94-95 -0.3868490513738543 1102 96-97 -0.5604896898351228 1102 98-99 -0.6251972809001209 1102 100-101 -0.5273988944770416 1103 1 0.3294108949739609 1103 2 0.18994957488376585 1103 3 0.3471307233486769 1103 4 0.3375957053460823 1103 5 0.43468615405026156 1103 6 0.30121720706507915 1103 7 0.34216199448101037 1103 8 0.3605230906810988 1103 9 0.37591376559721823 1103 10-11 0.5234530918589471 1103 12-13 0.4364444563695855 1103 14-15 0.6048227104889676 1103 16-17 0.6066203738988563 1103 18-19 0.6009147714270071 1103 20-21 0.5824020486125647 1103 22-23 0.6193637364497846 1103 24-25 0.5354448781582661 1103 26-27 0.5759595024526476 1103 28-29 0.5110727097364887 1103 30-31 0.42699962732289976 1103 32-33 0.4454784148989148 1103 34-35 0.5587016608096462 1103 36-37 0.7299298696641614 1103 38-39 0.8417046825606107 1103 40-41 0.7657112682175296 1103 42-43 0.47801556904656195 1103 44-45 0.6485248277510749 1103 46-47 0.5588577260165337 1103 48-49 0.653941902781078 1103 50-51 0.4223865010252652 1103 52-53 0.40763554839543303 1103 54-55 0.3570485017918976 1103 56-57 0.5088387184858654 1103 58-59 0.5048463660373699 1103 60-61 0.43392523365599445 1103 62-63 0.11182829366448388 1103 64-65 0.1841016639128945 1103 66-67 0.0972284282367255 1103 68-69 -6.676046740806175 1103 70-71 -7.810907594195729 1103 72-73 -1.1669268087652824 1103 74-75 0.21030567050933158 1103 76-77 0.5462736037802784 1103 78-79 0.43903660950185497 1103 80-81 0.11619816299548802 1103 82-83 0.14789042082550097 1103 84-85 -0.21147605639277955 1103 86-87 -0.11984117634805003 1103 88-89 -0.16732048099950347 1103 90-91 -0.36706966608122116 1103 92-93 -0.21695870759810987 1103 94-95 -0.04994084272282251 1103 96-97 -0.21245298648308975 1103 98-99 -0.17050700851725153 1103 100-101 -0.1808472891924282 1104 1 0.1186882219989478 1104 2 0.2531309201418601 1104 3 0.11869010208370057 1104 4 0.19626485245975545 1104 5 0.49517992688400625 1104 6 0.41460256706624676 1104 7 0.4744104357294674 1104 8 0.5678548752524577 1104 9 0.5845239616304312 1104 10-11 0.47902911548590765 1104 12-13 0.3734519012512081 1104 14-15 0.6522836411725095 1104 16-17 0.4226683450560742 1104 18-19 0.49650649005205594 1104 20-21 0.46605256961265695 1104 22-23 0.49255518254937414 1104 24-25 0.5120079669419795 1104 26-27 0.6830223527513013 1104 28-29 0.6176051014710922 1104 30-31 0.41497155841462785 1104 32-33 0.5615553937306501 1104 34-35 0.3143207280826701 1104 36-37 0.8171297970311713 1104 38-39 0.8603990792490279 1104 40-41 0.8423524779311364 1104 42-43 0.5620604376218168 1104 44-45 0.6950769608899776 1104 46-47 0.6379807736887599 1104 48-49 0.7186178893328403 1104 50-51 0.5377604167780703 1104 52-53 0.3535388908787027 1104 54-55 0.39609341336022297 1104 56-57 0.5260114135390843 1104 58-59 0.6642136952524744 1104 60-61 0.8599004049074921 1104 62-63 0.47764048578987683 1104 64-65 0.5028780509071389 1104 66-67 0.1805081466877816 1104 68-69 -6.731739326222016 1104 70-71 -4.379133164144687 1104 72-73 0.0016165052312899775 1104 74-75 0.6961977488093147 1104 76-77 0.8575657831392576 1104 78-79 0.4512207179495853 1104 80-81 0.399914842737104 1104 82-83 0.2311973260682798 1104 84-85 0.22166618540730454 1104 86-87 0.14846817949948843 1104 88-89 0.2586345846711424 1104 90-91 0.1450175815178696 1104 92-93 0.004123271074295332 1104 94-95 0.30899043564312123 1104 96-97 0.007525081995254368 1104 98-99 -0.034616801680140696 1104 100-101 0.05528630897819298 1105 1 0.23886329554418495 1105 2 0.20486515276006756 1105 3 0.19350390706324916 1105 4 0.2736760600367063 1105 5 0.42668311982366447 1105 6 0.29542340368819 1105 7 0.25959519572085554 1105 8 0.3984812654368568 1105 9 0.27929902082537694 1105 10-11 0.5320075182985633 1105 12-13 0.43826355368717174 1105 14-15 0.5575315424425114 1105 16-17 0.5797796315596244 1105 18-19 0.6774851798705228 1105 20-21 0.5977537965625004 1105 22-23 0.5512105603207758 1105 24-25 0.7101395591067146 1105 26-27 0.5886462316348542 1105 28-29 0.7579853841718176 1105 30-31 0.632227782853171 1105 32-33 0.8208378792009796 1105 34-35 0.6381623008745194 1105 36-37 0.8534604277264464 1105 38-39 0.7473718854483522 1105 40-41 0.7751500670823148 1105 42-43 0.565721196752186 1105 44-45 0.7120777501559559 1105 46-47 0.7755254389682307 1105 48-49 0.8712963710720985 1105 50-51 0.5699940395089342 1105 52-53 0.5476452265319978 1105 54-55 0.5133037168688652 1105 56-57 0.6789539406252558 1105 58-59 0.8719298465045355 1105 60-61 0.8764592052231954 1105 62-63 0.6766847564363552 1105 64-65 0.7112485754102593 1105 66-67 0.7324590686939523 1105 68-69 -2.795171915731956 1105 70-71 -2.8617061712480805 1105 72-73 0.8916961493440851 1105 74-75 1.4320117690432248 1105 76-77 1.5607415024475735 1105 78-79 1.2013979440384155 1105 80-81 0.9599722438994789 1105 82-83 0.8097909975901239 1105 84-85 0.915375018011165 1105 86-87 0.9154897766868295 1105 88-89 0.9726619937261738 1105 90-91 0.9011372629414218 1105 92-93 0.7163267581949064 1105 94-95 0.8074032935034907 1105 96-97 0.8396969984765619 1105 98-99 0.6705837436309032 1105 100-101 0.7820969568938132 1106 1 0.07640784401896639 1106 2 0.14960692261811914 1106 3 0.05289985994757629 1106 4 0.11620399721580554 1106 5 0.3129470884132175 1106 6 0.21025276037801177 1106 7 0.33340673302076596 1106 8 0.3083060917733107 1106 9 0.3274747985360378 1106 10-11 0.3418595267552291 1106 12-13 0.25909864882459743 1106 14-15 0.1831522008538613 1106 16-17 0.229966886165478 1106 18-19 0.33150964377870906 1106 20-21 0.4220712233217867 1106 22-23 0.3505717804959474 1106 24-25 0.24779283606232383 1106 26-27 0.48507782329899385 1106 28-29 0.42143600332011033 1106 30-31 0.17135493477101704 1106 32-33 0.4267394195211267 1106 34-35 0.4463728112943315 1106 36-37 0.5484483467841272 1106 38-39 0.6769339512894916 1106 40-41 0.44222194868908105 1106 42-43 0.2673038001952577 1106 44-45 0.7441466115271425 1106 46-47 0.8156553090981049 1106 48-49 0.9088182677800418 1106 50-51 0.6670281681709653 1106 52-53 0.423957518890802 1106 54-55 0.27793524812830483 1106 56-57 0.3994900324404185 1106 58-59 0.6758576628742148 1106 60-61 0.8956105190256736 1106 62-63 0.4122283988404618 1106 64-65 0.6399921574096545 1106 66-67 0.8459459306691848 1106 68-69 -3.113035099060255 1106 70-71 -3.5594606260955572 1106 72-73 0.7437438690662219 1106 74-75 1.2575946745098499 1106 76-77 1.3036122363648204 1106 78-79 1.1620488048055542 1106 80-81 0.9436070974180524 1106 82-83 0.7108163675689845 1106 84-85 0.6674031062020411 1106 86-87 0.6498026730927542 1106 88-89 0.8475638360698774 1106 90-91 0.8261991777707749 1106 92-93 0.5467964048273437 1106 94-95 0.8567796148565527 1106 96-97 0.6999914714454576 1106 98-99 0.5552439671283338 1106 100-101 0.7057438913547003 1107 1 0.027717737562344524 1107 2 0.07044267258537573 1107 3 0.07388949492671415 1107 4 -0.14388973699811203 1107 5 0.2434392398098737 1107 6 0.2527056126296898 1107 7 0.2329160783780253 1107 8 -0.027194806279034367 1107 9 0.2201575428343432 1107 10-11 0.09311773551497282 1107 12-13 0.18483334451581612 1107 14-15 0.014329467968316578 1107 16-17 0.07357071879097532 1107 18-19 0.19926500402866765 1107 20-21 0.2195726966889211 1107 22-23 0.36407589189185785 1107 24-25 0.2863845309490429 1107 26-27 0.5731541904901505 1107 28-29 0.46739277987346384 1107 30-31 0.35495577388868327 1107 32-33 0.3087583768449278 1107 34-35 0.14875849671407337 1107 36-37 0.41660245163173926 1107 38-39 0.5175612315005296 1107 40-41 0.40625425050950525 1107 42-43 0.2315185188990725 1107 44-45 0.44371477463590736 1107 46-47 0.6043721232573986 1107 48-49 0.531149836191048 1107 50-51 0.2747886033521709 1107 52-53 0.06324918010055569 1107 54-55 0.22132520865142524 1107 56-57 0.5387647382347254 1107 58-59 0.6971624347127872 1107 60-61 0.7103357711351634 1107 62-63 0.4881436404726749 1107 64-65 0.2963567526312687 1107 66-67 0.4917344393410019 1107 68-69 -2.294316554279252 1107 70-71 -2.5933737956590655 1107 72-73 1.2023305591820304 1107 74-75 1.4170334619520872 1107 76-77 0.9623880085962924 1107 78-79 1.0014786818846417 1107 80-81 0.7825008325674432 1107 82-83 0.713067345136384 1107 84-85 0.6422026814981159 1107 86-87 0.6612651186015519 1107 88-89 0.8116481734756249 1107 90-91 0.65973383230126 1107 92-93 0.6787259077179506 1107 94-95 1.1158747335678498 1107 96-97 1.0157784833633556 1107 98-99 0.9132078672948474 1107 100-101 1.1787461926138434 1108 1 -0.05612192308769082 1108 2 -0.03255244662808465 1108 3 0.013219163494248676 1108 4 0.05455382327007641 1108 5 -0.14826768248840239 1108 6 -0.25937796708146976 1108 7 -0.1564181001565359 1108 8 -0.015520881906169848 1108 9 0.18447117596355866 1108 10-11 0.11559240453350839 1108 12-13 0.0032053842235697516 1108 14-15 0.1327007560148914 1108 16-17 0.20142984997088575 1108 18-19 0.0984136851138615 1108 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5.406922250689693 2311 72-73 4.309251040496097 2311 74-75 3.2774233358936407 2311 76-77 2.8531791367557133 2311 78-79 2.2745615430293533 2311 80-81 1.7925397483374823 2311 82-83 1.8264554841826701 2311 84-85 1.6999372902712757 2311 86-87 1.8087141063140386 2311 88-89 1.7868355899141584 2311 90-91 1.968374486149866 2311 92-93 2.0970218904008284 2311 94-95 2.2268539148246127 2311 96-97 2.340727550658194 2311 98-99 2.592412202941375 2311 100-101 2.500788912420301 2312 1 -0.22260672379817237 2312 2 -0.11962780109458748 2312 3 -0.13418606254161247 2312 4 -0.11132255836616878 2312 5 -1.6225901600879098 2312 6 -0.42183274441902086 2312 7 -0.03860596482072509 2312 8 0.13797283769439161 2312 9 -2.3261748669889357 2312 10-11 -0.3017366099136467 2312 12-13 -1.2789539097005047 2312 14-15 -4.422374508952906 2312 16-17 -1.180007602482931 2312 18-19 -0.23950846478269483 2312 20-21 -0.08299088428856294 2312 22-23 -0.07040728991410106 2312 24-25 0.02695497773064659 2312 26-27 0.0784472816486641 2312 28-29 -0.16271370397407736 2312 30-31 -0.31158957000673126 2312 32-33 0.13154111213825814 2312 34-35 0.14332671093254845 2312 36-37 0.16540295328599086 2312 38-39 0.3192480943458449 2312 40-41 0.026034289243135333 2312 42-43 -0.2926277946819127 2312 44-45 0.3610401707141264 2312 46-47 0.46193190484278546 2312 48-49 0.27577689055261345 2312 50-51 0.4329727629131881 2312 52-53 0.5589757045646024 2312 54-55 0.5557961180846753 2312 56-57 0.3879633354857184 2312 58-59 0.3055703549322786 2312 60-61 0.25488280290211307 2312 62-63 0.33599395787321384 2312 64-65 0.48643647261943457 2312 66-67 1.0227381513134617 2312 68-69 3.5522569320160393 2312 70-71 4.2538133092714006 2312 72-73 2.637522344425598 2312 74-75 1.9325008544259283 2312 76-77 1.6850382695102972 2312 78-79 1.1927242772796411 2312 80-81 1.089672437323074 2312 82-83 0.9622654380432891 2312 84-85 0.8749591572761553 2312 86-87 0.8289990333875785 2312 88-89 0.7325611371359564 2312 90-91 0.9077744555360603 2312 92-93 1.0795113395709777 2312 94-95 1.5156279411962217 2312 96-97 1.4635889575812868 2312 98-99 1.6044232020448277 2312 100-101 1.434633569217656 2313 1 -0.3339551341905427 2313 2 -0.23038705465072695 2313 3 -0.1941361181098138 2313 4 0.019268294527620355 2313 5 0.041661856212094506 2313 6 -0.019363864843569445 2313 7 -2.732044676888176 2313 8 -2.7755550066610866 2313 9 -0.8223196835246043 2313 10-11 -0.06470587110204207 2313 12-13 -0.174944295732125 2313 14-15 -0.7319909321460472 2313 16-17 -0.24491304727403218 2313 18-19 0.15835651118238303 2313 20-21 -2.2658018988434137 2313 22-23 -0.5374764355027821 2313 24-25 0.18010618572532877 2313 26-27 -1.3040951532651235 2313 28-29 -1.7061283141709538 2313 30-31 -1.0959107452853516 2313 32-33 -0.9557963676094801 2313 34-35 -1.504373122586017 2313 36-37 -2.4870737370808484 2313 38-39 -2.209047055375869 2313 40-41 -1.1802230473216326 2313 42-43 -0.16515623712083993 2313 44-45 -0.4434172247663284 2313 46-47 -0.3260480682880882 2313 48-49 -2.304855582701002 2313 50-51 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2314 9 0.023473733284475884 2314 10-11 -0.07467507268078322 2314 12-13 -2.000050342617449 2314 14-15 -0.5415979061614351 2314 16-17 -1.4594608188709373 2314 18-19 -0.3888369060806198 2314 20-21 -0.687373163031225 2314 22-23 -0.33747166935063433 2314 24-25 -0.0900234309171637 2314 26-27 -1.9994960434007822 2314 28-29 -0.6940812972148009 2314 30-31 -0.04735330066917953 2314 32-33 0.09747048924936053 2314 34-35 -0.001931037008617409 2314 36-37 -0.31529707443137056 2314 38-39 -0.5039856287530498 2314 40-41 -0.4952798893600061 2314 42-43 -0.43452951080918467 2314 44-45 -3.63852703918144 2314 46-47 -4.287899559526316 2314 48-49 -4.079019974097413 2314 50-51 -4.3674822245670235 2314 52-53 -1.1749981424637674 2314 54-55 -1.6113419002651561 2314 56-57 -1.2761780371342333 2314 58-59 -2.154325798931943 2314 60-61 -1.4049717749249382 2314 62-63 -1.2905357819124674 2314 64-65 -1.1840869391524578 2314 66-67 -1.1409422440582127 2314 68-69 -1.7740861853881107 2314 70-71 1.3018095556834126 2314 72-73 0.9746869761370718 2314 74-75 0.5307847271515804 2314 76-77 0.6204392081421872 2314 78-79 -1.8603333231488044 2314 80-81 -1.319875605314678 2314 82-83 -0.03656420825503304 2314 84-85 0.26097328373307604 2314 86-87 0.07511742878743632 2314 88-89 -0.38508906510680063 2314 90-91 0.15426398166319188 2314 92-93 -0.20012098262686706 2314 94-95 -0.027369785040843198 2314 96-97 0.36828528808494454 2314 98-99 0.1078203401101554 2314 100-101 0.24082816386303918 2315 1 -0.41786733395240105 2315 2 -0.4184570254557265 2315 3 -0.5080743885575991 2315 4 -0.38715842462756456 2315 5 -0.2615634799122972 2315 6 -0.13502085654330642 2315 7 -0.8582896302490752 2315 8 -2.863096620821878 2315 9 -0.7239852913951026 2315 10-11 -1.355772408630294 2315 12-13 -0.7497126623741508 2315 14-15 -0.43256250850260614 2315 16-17 -0.31518153021004736 2315 18-19 -0.26264254110825647 2315 20-21 -0.6113174631470599 2315 22-23 -0.537268147732405 2315 24-25 -5.1923262335718405 2315 26-27 -1.28688537623718 2315 28-29 -1.0933396186425455 2315 30-31 -1.037597112503633 2315 32-33 -0.0528419791597301 2315 34-35 -2.4225012751082105 2315 36-37 -4.187405782501468 2315 38-39 -1.449805153430809 2315 40-41 -0.5409757298945408 2315 42-43 -1.8033083080219967 2315 44-45 -5.286756181869464 2315 46-47 -3.4552272756142663 2315 48-49 -2.352600346867174 2315 50-51 -0.9815831303661113 2315 52-53 -0.07258176475882294 2315 54-55 -1.1750943468483435 2315 56-57 -1.2703305967443903 2315 58-59 -0.7362651736053749 2315 60-61 -1.154030289521618 2315 62-63 -0.2501255963314435 2315 64-65 0.03133965848006781 2315 66-67 0.32004761883569444 2315 68-69 2.95362752753557 2315 70-71 3.5141842425146734 2315 72-73 -2.297817630807998 2315 74-75 -1.9424569301063244 2315 76-77 -0.191270409989869 2315 78-79 -0.18127662331700023 2315 80-81 -0.29844250132378036 2315 82-83 -0.7918294623282058 2315 84-85 -0.3880572786126244 2315 86-87 -0.11688073696027956 2315 88-89 0.035457118223426676 2315 90-91 -0.05203641916843438 2315 92-93 -0.8939351312159154 2315 94-95 0.0017109086621687197 2315 96-97 -0.799455621952653 2315 98-99 -3.4105632890054913 2315 100-101 -4.850714849109483 2316 1 -0.5845279279195665 2316 2 -0.3830610461543422 2316 3 -1.0541893969129177 2316 4 -0.4652819176157408 2316 5 -0.19110873474063084 2316 6 -0.7536281793615771 2316 7 -0.7174515834292734 2316 8 -1.1639131488523873 2316 9 -5.176177071531949 2316 10-11 -1.6767817341534226 2316 12-13 -0.8427485620302946 2316 14-15 -0.5043035943155658 2316 16-17 -0.47743834538636776 2316 18-19 -0.6983069337275722 2316 20-21 -3.952584242551602 2316 22-23 -1.1335540132742636 2316 24-25 -0.4521648998069807 2316 26-27 -0.5060287398125638 2316 28-29 0.018853147414922944 2316 30-31 -0.04544232582328789 2316 32-33 -1.6067393305339834 2316 34-35 -0.8614013449085292 2316 36-37 -0.2933872486225937 2316 38-39 -5.8760179251037385 2316 40-41 -5.377046421832212 2316 42-43 -3.6137039836884064 2316 44-45 -2.254850933128118 2316 46-47 -2.4969013545465018 2316 48-49 -2.8782996539364376 2316 50-51 -1.2869441809288347 2316 52-53 -0.8843334385019688 2316 54-55 -0.23543090299902403 2316 56-57 -3.888080240062596 2316 58-59 -0.8987277461294809 2316 60-61 -0.8241758985669385 2316 62-63 -0.34369696519650006 2316 64-65 -0.26191528701924227 2316 66-67 -0.07088741256730913 2316 68-69 2.3854250394434224 2316 70-71 3.3172280223914647 2316 72-73 2.0803843786125498 2316 74-75 0.8733109413124787 2316 76-77 -1.4338079553475787 2316 78-79 -0.04243363689198887 2316 80-81 -0.09131951058357402 2316 82-83 0.1152603786170765 2316 84-85 0.5301345286727575 2316 86-87 0.40281195586758045 2316 88-89 0.3160737044089643 2316 90-91 0.3729082299617055 2316 92-93 0.37627141915498186 2316 94-95 0.4783074392217763 2316 96-97 0.743103999453826 2316 98-99 0.9616442515726433 2316 100-101 1.2938796981779142 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 35.0 4 79.0 5 211.0 6 476.0 7 1031.0 8 2167.0 9 3507.0 10 5571.0 11 8109.0 12 10611.0 13 13003.0 14 15932.0 15 19879.0 16 23811.0 17 26117.0 18 23678.0 19 21367.0 20 20680.0 21 20912.0 22 21814.0 23 24292.0 24 28740.0 25 35026.0 26 43421.0 27 53261.0 28 64831.0 29 77059.0 30 89700.0 31 103234.0 32 118039.0 33 134934.0 34 152972.0 35 167576.0 36 163121.0 37 123210.0 38 45671.0 39 2171.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.533149527790236 20.68694770546212 15.724077985894635 22.05582478085301 2 16.308830220572435 21.668453447847398 38.676109194940025 23.346607136640138 3 19.333076468808965 25.642341356148663 30.77783139124548 24.246750783796887 4 13.223047672931724 18.202959970545642 36.20146923419276 32.37252312232987 5 13.208318304603203 38.19762064944519 33.8772083231058 14.716852722845811 6 31.172697063411135 36.96647507911464 16.920419648156674 14.940408209317551 7 27.518510094205382 33.98043394370483 20.38135865321773 18.119697308872063 8 26.43842201634446 35.2528097473793 19.069913152444435 19.238855083831808 9 26.143208744410362 17.00756127670888 20.40464443274821 36.44458554613255 10-11 25.030670612031763 27.31073951587249 26.53269031084755 21.125899561248193 12-13 26.048835320926656 24.932948357782404 27.469988051034267 21.54822827025668 14-15 22.75737838131424 26.08235364017349 26.538886951741425 24.621381026770845 16-17 21.526889219105964 29.316114566654107 27.206879664682965 21.950116549556967 18-19 21.165425006624154 26.99366031698115 30.743426633630417 21.09748804276428 20-21 22.17499383346947 26.061738523501532 30.907641276564846 20.855626366464154 22-23 21.7244476292416 26.24534921090749 30.805260556717347 21.224942603133563 24-25 21.583955308979256 26.440256728500128 30.91713099779378 21.058656964726836 26-27 21.39412573881396 26.669953618567938 30.86456837900256 21.07135226361554 28-29 21.121554837968194 26.77377063151189 31.011117002649392 21.093557527870527 30-31 21.300034178575405 26.478643491652054 30.84353864384574 21.3777836859268 32-33 21.17010860089957 26.66878120989282 30.68864391262115 21.472466276586456 34-35 21.708297398206813 26.700984337386274 30.516529558029454 21.074188706377456 36-37 21.641679426087364 26.40911758469422 30.525780236823657 21.423422752394757 38-39 21.306246029493263 26.797777867888566 30.68227385570559 21.21370224691258 40-41 21.54820109315875 26.5706048411316 30.710763409213765 21.17043065649589 42-43 21.406145353743764 26.742620139533347 30.21650794222851 21.63472656449438 44-45 21.583006051891115 26.987091641506765 29.946995272551334 21.482907034050786 46-47 21.710709473044997 27.207934041954463 29.430651462736122 21.650705022264415 48-49 21.77470624819629 27.305501575555336 29.223916248265358 21.69587592798302 50-51 21.705199847204636 27.477158775042465 29.051269330655412 21.766372047097487 52-53 22.065330738703768 27.51294187094971 28.781922305837405 21.63980508450912 54-55 21.62048561187258 27.477785146784512 28.928772261764337 21.97295697957858 56-57 21.82044666963826 27.924371425269655 28.53360828084267 21.72157362424941 58-59 21.984228032610044 27.33789672284762 28.692926866310454 21.984948378231874 60-61 21.885247131628464 27.371032073540754 29.112733078633745 21.630987716197033 62-63 21.7786366746288 27.484395818079243 29.017686740046333 21.71928076724562 64-65 21.61709020872708 27.4371348986614 28.87620360028257 22.06957129232895 66-67 21.526920523222596 27.687581323337536 28.78199168799972 22.003506465440157 68-69 21.93606384725426 26.922150024961123 29.435285985516803 21.706500142267814 70-71 21.4764616870144 26.993429844232907 29.745886534875858 21.784221933876825 72-73 21.823429671495173 27.271681207205617 28.92888997195312 21.97599914934609 74-75 21.648518170564337 27.712358722012 28.439589632913677 22.199533474509987 76-77 20.998706037060323 27.77287116333072 28.97241059311496 22.256012206493992 78-79 21.57716086434574 27.75267106842737 28.388835534213687 22.281332533013202 80-81 21.391182799764614 28.019402389828507 28.359790455630268 22.22962435477661 82-83 21.618321572769705 27.99501270080856 28.158555303656602 22.22811042276513 84-85 21.401069528344664 27.84398605610148 28.069552650760077 22.685391764793785 86-87 21.578079978586274 27.80243437095079 28.232809453247796 22.386676197215134 88-89 21.433906348134173 27.810817793656454 28.002416210576413 22.752859647632956 90-91 21.381785554060116 27.915423176702365 28.16515647342495 22.537634795812572 92-93 21.55019033029095 27.737477737282678 28.139324473759626 22.573007458666744 94-95 21.185392077364327 27.562813466678683 28.3416824338529 22.910112022104094 96-97 21.298005467769908 27.460939247234517 28.273990088128535 22.967065196867033 98-99 21.186621256938707 27.428067917715076 28.02839228434781 23.356918540998407 100-101 21.036278386897635 27.327201152207405 28.351018700561426 23.28550176033354 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4513.0 1 3005.0 2 1499.5 3 1565.5 4 1443.5 5 1344.5 6 1945.5 7 3369.0 8 4896.0 9 5608.5 10 5035.0 11 4007.0 12 3594.5 13 3517.5 14 3504.5 15 3542.5 16 3599.5 17 3713.5 18 3809.0 19 4103.5 20 4663.0 21 5182.5 22 5909.5 23 6938.5 24 7987.5 25 9351.5 26 11319.0 27 13500.5 28 15521.0 29 17782.5 30 20408.5 31 23578.0 32 27046.0 33 30037.5 34 33088.0 35 36099.5 36 39877.0 37 43547.0 38 45185.0 39 45553.5 40 45860.0 41 47896.0 42 50548.0 43 52700.5 44 54226.0 45 55454.5 46 56496.5 47 58252.5 48 62233.0 49 63671.0 50 61227.5 51 58422.5 52 56046.5 53 54858.0 54 53482.0 55 49406.0 56 44760.0 57 40552.0 58 36208.0 59 31828.5 60 27065.0 61 22055.0 62 17698.0 63 13911.5 64 10589.5 65 8112.0 66 6182.0 67 4782.5 68 3496.0 69 2427.0 70 1745.0 71 1380.5 72 1142.5 73 1014.0 74 863.0 75 649.0 76 427.5 77 256.0 78 147.0 79 86.0 80 57.0 81 42.5 82 27.5 83 14.0 84 9.0 85 6.5 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021725392242354553 2 0.01890469214459029 3 3.0007447848556015E-4 4 0.07825942398903409 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.00978242799862926 12-13 0.0 14-15 0.0 16-17 5.401340612740082E-4 18-19 1.5003723924278007E-4 20-21 0.0 22-23 2.1005213493989207E-4 24-25 0.003420849054735385 26-27 0.004141027803100729 28-29 0.0012903202574879086 30-31 2.700670306370041E-4 32-33 8.702159876081243E-4 34-35 0.003360834159038273 36-37 0.08822189667475468 38-39 0.0981543619126267 40-41 0.08120015387819257 42-43 0.08510112209850484 44-45 0.0841108763195025 46-47 0.0826105039270747 48-49 0.07708913352294039 50-51 0.07687908138800051 52-53 0.0611251712675086 54-55 0.052182951808638904 56-57 0.028837157382462328 58-59 0.02316574973908524 60-61 0.014283545175912661 62-63 0.0019804915580046967 64-65 0.006361578943893874 66-67 0.16531103019769508 68-69 0.7610788997829261 70-71 0.9275302129988663 72-73 0.38130463981160123 74-75 0.05782435200416743 76-77 0.2119426041543511 78-79 0.01518376861136934 80-81 0.00399099056385795 82-83 0.0012303053617907964 84-85 2.700670306370041E-4 86-87 0.0019204766623075846 88-89 4.501117177283402E-4 90-91 0.004531124625131958 92-93 0.004231050146646397 94-95 0.08420089866304817 96-97 0.04048004714770206 98-99 0.01422353028021555 100-101 0.014013478145275657 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1666253.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.584351700015446 #Duplication Level Percentage of deduplicated Percentage of total 1 78.01434815701329 43.36376965606878 2 12.730164306876613 14.151958600648259 3 4.060089556064401 6.770323374535299 4 1.7573160258514038 3.9071708811599146 5 0.881359435651506 2.4494896422690218 6 0.5439286100000285 1.8140351494765272 7 0.37151999040268896 1.4455488467090598 8 0.24448661464763594 1.0871703979616274 9 0.18160049207534915 0.9084731058370867 >10 1.0588575446803365 11.118366128235845 >50 0.09752552642872224 3.7899487948691997 >100 0.055080800012098856 5.562788393171659 >500 0.002627957855988362 0.9282351719020306 >1k 6.569894639970905E-4 0.6162468766315692 >5k 2.1899648799903018E-4 0.7721801603777498 >10k+ 2.1899648799903018E-4 1.3142948201463804 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11116 0.6671255805690972 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10478 0.6288360771143398 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7259 0.43564812786533613 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5428 0.32576085384392406 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2730 0.16384066525311583 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2222 0.13335309823898292 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1754 0.1052661270527345 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.0014895697112025E-5 0.0 14-15 0.0 0.0 0.0 6.0014895697112025E-5 6.0014895697112025E-5 16-17 0.0 0.0 0.0 6.0014895697112025E-5 1.2002979139422405E-4 18-19 0.0 0.0 0.0 6.0014895697112025E-5 1.2002979139422405E-4 20-21 0.0 0.0 0.0 1.8004468709133608E-4 1.8004468709133608E-4 22-23 0.0 0.0 0.0 3.600893741826721E-4 2.400595827884481E-4 24-25 0.0 0.0 0.0 7.801936440624564E-4 2.400595827884481E-4 26-27 0.0 0.0 0.0 0.0014403574967306884 2.400595827884481E-4 28-29 0.0 0.0 0.0 0.0029707373370070453 2.400595827884481E-4 30-31 0.0 0.0 0.0 0.007081757692259219 2.400595827884481E-4 32-33 0.0 0.0 0.0 0.014703649445792446 2.400595827884481E-4 34-35 0.0 0.0 0.0 0.027786896707762868 2.400595827884481E-4 36-37 0.0 0.0 0.0 0.04705167822653583 2.400595827884481E-4 38-39 0.0 0.0 0.0 0.07402837384238767 2.400595827884481E-4 40-41 0.0 0.0 0.0 0.1045759557522177 2.400595827884481E-4 42-43 0.0 0.0 0.0 0.13668392495017262 2.400595827884481E-4 44-45 0.0 0.0 0.0 0.1759336667360839 2.400595827884481E-4 46-47 0.0 0.0 0.0 0.22640619401735512 2.400595827884481E-4 48-49 0.0 0.0 0.0 0.279129279887268 2.400595827884481E-4 50-51 0.0 0.0 0.0 0.33695363189143546 2.400595827884481E-4 52-53 3.0007447848556013E-5 0.0 0.0 0.3920473061413843 2.400595827884481E-4 54-55 6.0014895697112025E-5 0.0 0.0 0.45422273808359237 2.400595827884481E-4 56-57 6.0014895697112025E-5 0.0 0.0 0.5285211789566171 2.400595827884481E-4 58-59 6.0014895697112025E-5 0.0 0.0 0.6072007072155309 2.400595827884481E-4 60-61 6.0014895697112025E-5 0.0 0.0 0.6863903620878702 2.400595827884481E-4 62-63 6.0014895697112025E-5 0.0 0.0 0.7644097264941159 2.400595827884481E-4 64-65 6.0014895697112025E-5 0.0 0.0 0.8462100293292796 2.400595827884481E-4 66-67 6.0014895697112025E-5 3.0007447848556013E-5 0.0 0.9379127899544667 2.400595827884481E-4 68-69 6.0014895697112025E-5 6.0014895697112025E-5 0.0 1.0469898628839678 2.400595827884481E-4 70-71 6.0014895697112025E-5 6.0014895697112025E-5 0.0 1.1782124323257033 2.400595827884481E-4 72-73 6.0014895697112025E-5 6.0014895697112025E-5 0.0 1.3234184724648657 2.400595827884481E-4 74-75 6.0014895697112025E-5 6.0014895697112025E-5 0.0 1.4828180354363953 2.400595827884481E-4 76-77 6.0014895697112025E-5 6.0014895697112025E-5 0.0 1.6683240780361686 2.400595827884481E-4 78-79 6.0014895697112025E-5 6.0014895697112025E-5 0.0 1.887468469674173 2.400595827884481E-4 80-81 6.0014895697112025E-5 6.0014895697112025E-5 0.0 2.1299586557383545 3.0007447848556015E-4 82-83 6.0014895697112025E-5 1.2002979139422405E-4 0.0 2.38025077824316 4.201042698797842E-4 84-85 6.0014895697112025E-5 1.2002979139422405E-4 0.0 2.6290125209076893 4.8011916557689615E-4 86-87 6.0014895697112025E-5 1.2002979139422405E-4 0.0 2.8802348742958 5.401340612740082E-4 88-89 6.0014895697112025E-5 1.2002979139422405E-4 0.0 3.1702268503042452 5.401340612740082E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6755 0.0 42.00879 1 GTATCAA 10660 0.0 34.00952 1 TATAGCG 70 0.008471567 27.090815 5 TATCAAC 13995 0.0 25.828724 2 ATCAACG 14045 0.0 25.699139 3 TCAACGC 14205 0.0 25.381617 4 CAACGCA 14450 0.0 24.967606 5 AACGCAG 14845 0.0 24.431004 6 GTACATG 8930 0.0 21.999523 1 ACGCAGA 16585 0.0 21.524822 7 TACATGG 8955 0.0 21.4541 2 AGTACTT 9915 0.0 21.321808 12-13 CGCAGAG 16720 0.0 21.294317 8 GAGTACT 9625 0.0 21.27477 12-13 GCAGAGT 17110 0.0 20.19936 9 ACATGGG 9020 0.0 20.139463 3 AGAGTAC 15000 0.0 20.052471 10-11 CGATTCG 95 8.321165E-6 19.982645 38-39 GTACTTT 10365 0.0 19.801609 14-15 TACTTTT 11250 0.0 18.833754 14-15 >>END_MODULE