FastQCFastQC Report
Fri 27 May 2016
SRR939535_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939535_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2837481
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87740.3092179295649909No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72340.2549444383944774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44730.15763982208162802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53350.060.024731
GTATCAA101400.045.8865851
TATCAAC130700.035.109462
ATCAACG132800.034.4002273
TCAACGC136500.033.5139124
CAACGCA137100.032.9058235
AACGCAG142200.031.7519326
GTGGTAT25050.028.8535161
TGGTATC24400.028.2399182
ACGCAGA160050.027.8007747
CGCAGAG161200.027.7793058
GTACATG103050.025.9611071
TACATGG105150.025.0038642
GCAGAGT166450.024.8079519
GAGTACT103800.022.67272812-13
ACATGGG109250.022.3776873
CAGAGTA162850.021.77970510-11
AGAGTAC154500.021.04770910-11
GTACTTT113800.020.74654614-15
GGACCGT3051.5279511E-1020.7156016
Produced by FastQC (version 0.11.5)