FastQCFastQC Report
Fri 27 May 2016
SRR939535_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939535_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2837481
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124980.44046109912277825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115870.40835515726801347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76090.2681603859197647No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33880.11940168057512984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61450.060.0786671
GTATCAA111200.041.8089181
GTGGTAT25500.035.8692131
TGGTATC24700.035.2985342
ATCAACG148500.031.0395223
TATCAAC149200.031.0285552
TCAACGC151100.030.5054234
CAACGCA153050.030.0548465
AACGCAG157750.029.0693046
ACGCAGA184400.024.8167487
CGCAGAG185700.024.4389348
GCAGAGT195700.022.5123469
GTACATG118750.022.1894151
GAGTACT121650.022.1392212-13
TACATGG117750.021.851172
ACATGGG119400.020.9884323
GTACTTT129000.020.78598814-15
CAGAGTA192500.020.5121510-11
AGAGTAC185200.020.34864410-11
AGTACTT125900.020.05605112-13
Produced by FastQC (version 0.11.5)