##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939535_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2837481 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.971821485324483 31.0 31.0 31.0 30.0 34.0 2 31.24647636407081 31.0 31.0 34.0 30.0 34.0 3 31.23237759125083 31.0 31.0 34.0 28.0 34.0 4 35.16852518131399 35.0 35.0 37.0 33.0 37.0 5 34.78239642838137 35.0 35.0 37.0 32.0 37.0 6 34.734333727697205 35.0 35.0 37.0 32.0 37.0 7 34.610203557310165 35.0 35.0 37.0 30.0 37.0 8 34.631512951099936 35.0 35.0 37.0 30.0 37.0 9 35.984908445201924 37.0 35.0 39.0 30.0 39.0 10-11 35.84025672771025 37.0 35.0 39.0 30.0 39.0 12-13 35.79794807436596 37.0 35.0 39.0 30.0 39.0 14-15 36.67070140733982 38.0 36.0 40.0 31.0 41.0 16-17 36.672025997707124 38.0 35.5 40.0 31.0 41.0 18-19 36.619606615868086 38.0 35.0 40.0 30.0 41.0 20-21 36.51323110181178 38.0 35.0 40.0 30.0 41.0 22-23 36.37703371405835 38.0 35.0 40.0 30.0 41.0 24-25 36.15832546543923 38.0 34.0 40.0 29.5 41.0 26-27 35.783475202124706 38.0 34.0 40.0 27.5 41.0 28-29 35.488389349567456 38.0 34.0 40.0 27.0 41.0 30-31 35.27088886233952 38.0 34.0 40.0 27.0 41.0 32-33 35.07061104550127 37.5 33.0 40.0 27.0 41.0 34-35 35.02209706426228 38.0 33.0 40.0 26.0 41.0 36-37 35.02872970779364 38.0 33.0 40.0 25.5 41.0 38-39 35.00252794644264 38.0 33.0 40.0 26.0 41.0 40-41 34.77442844551206 38.0 33.0 40.0 25.5 41.0 42-43 34.474625556964085 37.5 33.0 40.0 24.5 41.0 44-45 34.301381577533036 37.0 33.0 40.0 24.0 41.0 46-47 33.98181520863047 37.0 33.0 40.0 23.5 41.0 48-49 33.66832799937691 37.0 32.0 40.0 23.0 41.0 50-51 33.39575154864473 37.0 32.0 40.0 22.0 41.0 52-53 33.013499297440234 37.0 31.0 39.5 20.0 41.0 54-55 32.78318392264124 36.0 31.0 39.0 19.5 40.5 56-57 32.38712012520965 36.0 31.0 39.0 18.0 40.0 58-59 31.946446513650663 35.5 30.0 39.0 16.0 40.0 60-61 31.660575524558578 35.0 30.0 39.0 14.5 40.0 62-63 31.268723561496977 35.0 29.0 38.0 13.0 40.0 64-65 30.83384646452258 34.5 29.0 38.0 8.5 40.0 66-67 29.159646707766502 33.5 25.5 37.5 7.0 39.5 68-69 25.53793012182284 30.0 16.0 35.0 4.0 39.0 70-71 24.935122913598363 29.5 15.0 35.0 2.0 38.0 72-73 26.719916362435555 31.0 21.5 35.0 2.0 38.0 74-75 27.312821477923553 32.0 24.0 35.0 2.0 38.0 76-77 27.362990976855883 31.5 25.5 34.5 2.0 36.5 78-79 28.19193044816864 33.0 26.0 35.0 2.0 37.0 80-81 28.04678515909005 33.0 26.0 35.0 2.0 36.5 82-83 27.643542987600622 33.0 26.0 35.0 2.0 36.0 84-85 27.304343711905034 32.0 26.0 35.0 2.0 36.0 86-87 26.848116868447754 32.0 25.0 34.5 2.0 35.0 88-89 26.366465537566594 32.0 24.5 34.0 2.0 35.0 90-91 25.916374770438992 32.0 23.5 34.0 2.0 35.0 92-93 25.34718487982827 31.5 21.5 34.0 2.0 35.0 94-95 23.978067870762835 31.0 12.0 34.0 2.0 35.0 96-97 23.172103002628035 30.0 4.5 34.0 2.0 35.0 98-99 22.814291619926266 30.0 2.0 34.0 2.0 35.0 100-101 21.545569115705092 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.32816440014986625 1101 2 0.27039609622116245 1101 3 0.32706266931768013 1101 4 0.2600445964528788 1101 5 0.4143641583496773 1101 6 0.5191951340739607 1101 7 0.469828482913222 1101 8 0.5028541771927308 1101 9 0.6169766059888744 1101 10-11 0.7821280497270209 1101 12-13 0.6942987722847747 1101 14-15 0.8783434964702863 1101 16-17 0.8178662987880756 1101 18-19 0.8185272186893187 1101 20-21 0.852297379119058 1101 22-23 0.900427446797174 1101 24-25 0.7143764264218504 1101 26-27 0.8547497550031338 1101 28-29 0.7120248546281687 1101 30-31 0.9215101746158396 1101 32-33 0.7157667789977111 1101 34-35 0.9266691764450741 1101 36-37 0.8268290398976532 1101 38-39 0.8043785228389311 1101 40-41 1.0163617707466912 1101 42-43 1.1033086218806503 1101 44-45 0.5833280352071739 1101 46-47 0.765544813280961 1101 48-49 0.7558582185331346 1101 50-51 0.5713720005977336 1101 52-53 0.7784455771169405 1101 54-55 0.6761066436951637 1101 56-57 0.5936406287636586 1101 58-59 0.6052911906721157 1101 60-61 0.42284917003371447 1101 62-63 0.2830395278101534 1101 64-65 0.2771129081810173 1101 66-67 -2.6724683949760255 1101 68-69 -7.854438163378973 1101 70-71 -11.310024301375744 1101 72-73 -11.265259261020947 1101 74-75 -9.643536091244844 1101 76-77 -4.583860515706871 1101 78-79 -1.4311384016597266 1101 80-81 -0.7962884664300667 1101 82-83 -0.43568504366538363 1101 84-85 -0.43523625279988565 1101 86-87 -0.2663663696993588 1101 88-89 -0.44375241445099434 1101 90-91 -0.4421471044709442 1101 92-93 -0.9357565855636167 1101 94-95 -4.732596128410336 1101 96-97 -1.5659653484439815 1101 98-99 -0.7885702908134178 1101 100-101 -0.46064969365567876 1102 1 0.45503213075601323 1102 2 0.24218707823687424 1102 3 0.3957204452925289 1102 4 0.26050970923027705 1102 5 0.4304958549052742 1102 6 0.5103118232415582 1102 7 0.41712094197477256 1102 8 0.47530280498271793 1102 9 0.6154251598213918 1102 10-11 0.6030793930183336 1102 12-13 0.6025157794473586 1102 14-15 0.6049721606588676 1102 16-17 0.6495387074921197 1102 18-19 0.6275831449302487 1102 20-21 0.8267600747714496 1102 22-23 0.685532113225733 1102 24-25 0.6795433986982005 1102 26-27 0.6647906552559846 1102 28-29 0.6525684730395014 1102 30-31 0.5874973108873505 1102 32-33 0.4957364387202716 1102 34-35 0.552532502595966 1102 36-37 0.8228481240251639 1102 38-39 0.8832184600503723 1102 40-41 0.8631524482967734 1102 42-43 0.8747397919432061 1102 44-45 0.6363318488883749 1102 46-47 0.7485504825418232 1102 48-49 0.566036460466627 1102 50-51 0.6852639593613503 1102 52-53 0.6939251414941481 1102 54-55 0.4092863565851772 1102 56-57 0.3643542010002889 1102 58-59 0.30391693243343454 1102 60-61 0.3501696797911187 1102 62-63 0.3323644555300085 1102 64-65 0.29549538079184146 1102 66-67 -2.8649462558956813 1102 68-69 -7.847110893377298 1102 70-71 -11.043171408763984 1102 72-73 -9.265386195512562 1102 74-75 -7.685168500914319 1102 76-77 -3.9772122926458238 1102 78-79 -1.238964840304142 1102 80-81 -0.5938444488033916 1102 82-83 -0.39406459119180326 1102 84-85 -0.5016306312349492 1102 86-87 -0.32897590813166744 1102 88-89 -0.317161913645986 1102 90-91 -0.3439103258635221 1102 92-93 -0.8118494788042376 1102 94-95 -4.48480283998439 1102 96-97 -1.171500611211112 1102 98-99 -0.551026312136198 1102 100-101 -0.5402653539508613 1103 1 0.29038774146025403 1103 2 0.21405209604889208 1103 3 0.25719859261988987 1103 4 0.1898432994061352 1103 5 0.3514767716915088 1103 6 0.3963687706352488 1103 7 0.3932828522996914 1103 8 0.35725828041560703 1103 9 0.505554083757751 1103 10-11 0.6225809590641589 1103 12-13 0.5497455805023677 1103 14-15 0.6830418668051337 1103 16-17 0.6294238512613717 1103 18-19 0.5226500845135504 1103 20-21 0.6643468797869971 1103 22-23 0.5759329849172303 1103 24-25 0.7953812905930988 1103 26-27 0.6644034608580114 1103 28-29 0.6755954923440939 1103 30-31 0.7417930986627752 1103 32-33 0.703943844494205 1103 34-35 0.7908909828289978 1103 36-37 0.7578753488323713 1103 38-39 0.9465661985883997 1103 40-41 0.8255866547445265 1103 42-43 0.7512304099333491 1103 44-45 0.643984247423603 1103 46-47 0.6496723488145335 1103 48-49 0.6426067268113229 1103 50-51 0.5111795346796697 1103 52-53 0.659319400849185 1103 54-55 0.5394572519652527 1103 56-57 0.3842453747055643 1103 58-59 0.26955165988826835 1103 60-61 0.28014948776414883 1103 62-63 0.3178479369160385 1103 64-65 0.02468030333472626 1103 66-67 -3.27511608300534 1103 68-69 -8.166444432070243 1103 70-71 -11.637199637702485 1103 72-73 -9.594516181498488 1103 74-75 -7.898507358650086 1103 76-77 -4.149743971261174 1103 78-79 -1.3424500510869812 1103 80-81 -0.6319045319354224 1103 82-83 -0.4235959401020075 1103 84-85 -0.5531066248605043 1103 86-87 -0.3765558787907004 1103 88-89 -0.17047379862630052 1103 90-91 -0.28279463968533136 1103 92-93 -0.36377321287698194 1103 94-95 0.6604238038810664 1103 96-97 0.7562175417278887 1103 98-99 0.3296181724029488 1103 100-101 0.2622128403546604 1104 1 0.3439286096941956 1104 2 0.19537414467902892 1104 3 0.3443006127214261 1104 4 0.1765160416084015 1104 5 0.4037954371869077 1104 6 0.5242704219894279 1104 7 0.5301495367264835 1104 8 0.3506885455191622 1104 9 0.510517609468053 1104 10-11 0.6197994414504464 1104 12-13 0.5342756742151167 1104 14-15 0.7373542781801561 1104 16-17 0.7829924450980386 1104 18-19 0.7365716038807975 1104 20-21 0.7620623997174008 1104 22-23 0.5968546973414988 1104 24-25 0.8006563209848281 1104 26-27 0.9352067256294063 1104 28-29 0.9223900290830969 1104 30-31 0.8441288674091183 1104 32-33 0.780368873031243 1104 34-35 0.7706861737699882 1104 36-37 0.7893087635475382 1104 38-39 0.7776862960779809 1104 40-41 0.9547996607183364 1104 42-43 1.0029084359125804 1104 44-45 0.9900655269617502 1104 46-47 0.8158371234099135 1104 48-49 0.8664900204653918 1104 50-51 0.745838245261055 1104 52-53 0.9307056830934997 1104 54-55 0.8323700778649012 1104 56-57 0.9114287422102691 1104 58-59 0.9775697650967565 1104 60-61 0.8260167079452714 1104 62-63 0.9210460340818969 1104 64-65 0.6814468257285 1104 66-67 -2.5813206534829582 1104 68-69 -7.14475475944101 1104 70-71 -8.801231827643626 1104 72-73 -8.101974379172024 1104 74-75 -6.694737193042563 1104 76-77 -3.312366170458933 1104 78-79 -0.4266810855522998 1104 80-81 0.4255543296351014 1104 82-83 0.5146017840169712 1104 84-85 0.5043891198759489 1104 86-87 0.5688459706787761 1104 88-89 0.6427675003187154 1104 90-91 0.677601059779672 1104 92-93 0.6871140169485592 1104 94-95 1.705513684995033 1104 96-97 1.7983280533880261 1104 98-99 1.3744412301565028 1104 100-101 1.2880721022023742 1105 1 0.46226909113803316 1105 2 0.422792003386089 1105 3 0.37647991873492614 1105 4 0.3138287652450913 1105 5 0.5144905392850632 1105 6 0.5414094630683408 1105 7 0.5906235845403174 1105 8 0.5550080908497534 1105 9 0.7717166946751846 1105 10-11 0.8151904596223432 1105 12-13 0.8431574597552753 1105 14-15 0.9955764129447644 1105 16-17 0.945194796537173 1105 18-19 0.9241611618548689 1105 20-21 1.1304760150085684 1105 22-23 1.1415488780358203 1105 24-25 1.082769620602221 1105 26-27 1.1633701777122738 1105 28-29 1.1842779979454363 1105 30-31 1.0526978714666058 1105 32-33 1.2081231164471333 1105 34-35 1.2218249132234291 1105 36-37 1.185745967603367 1105 38-39 1.074241464616378 1105 40-41 1.1527127636593946 1105 42-43 1.199231858989826 1105 44-45 1.0272662773067651 1105 46-47 0.976758857797698 1105 48-49 0.8282484040007745 1105 50-51 0.9063484468129772 1105 52-53 0.982935180825109 1105 54-55 0.9835065447976632 1105 56-57 1.0995333876557254 1105 58-59 1.1059857768642836 1105 60-61 1.0212458090187226 1105 62-63 1.0416840855813447 1105 64-65 0.9847630531788951 1105 66-67 1.749451652340479 1105 68-69 -5.033640222332814 1105 70-71 -5.568001274222858 1105 72-73 -1.8544718793992772 1105 74-75 1.0010571459018287 1105 76-77 1.1202508023782478 1105 78-79 1.0865656440838514 1105 80-81 1.0806918274658806 1105 82-83 1.0930884505183656 1105 84-85 1.034387374166883 1105 86-87 1.0929147536819706 1105 88-89 1.231895709737696 1105 90-91 1.2722934844352842 1105 92-93 1.3559113266972602 1105 94-95 2.1647774089540093 1105 96-97 2.291396916962409 1105 98-99 1.8863782648062504 1105 100-101 1.7309084455926431 1106 1 0.1536186526061627 1106 2 0.1688845073733276 1106 3 0.18827698174754914 1106 4 0.035184091087323566 1106 5 0.29534971536730836 1106 6 0.3299727380600004 1106 7 0.35131055317746984 1106 8 0.20638960194293787 1106 9 0.2918302244108233 1106 10-11 0.4420715713452239 1106 12-13 0.3277303920122421 1106 14-15 0.41515641496099676 1106 16-17 0.3682815025463313 1106 18-19 0.36795696342598916 1106 20-21 0.5243133045636768 1106 22-23 0.5549232599165563 1106 24-25 0.5799295677664276 1106 26-27 0.6278693880188726 1106 28-29 0.7731258727525372 1106 30-31 0.7333037252789723 1106 32-33 0.7538953487074593 1106 34-35 0.8114050961841457 1106 36-37 0.7691081228071965 1106 38-39 0.6555124186332151 1106 40-41 0.8024125250711762 1106 42-43 0.8162370210773062 1106 44-45 0.8195950068955753 1106 46-47 0.7064611524321762 1106 48-49 0.6398453941186233 1106 50-51 0.5794456217526829 1106 52-53 0.8444839621640483 1106 54-55 0.8153768127764351 1106 56-57 0.8203730217342908 1106 58-59 0.9525947480915242 1106 60-61 0.8641723016168044 1106 62-63 0.8767265687599703 1106 64-65 0.9040566459349151 1106 66-67 1.736408140363249 1106 68-69 -5.004829466993012 1106 70-71 -6.0033666140495185 1106 72-73 -2.0945241982027696 1106 74-75 0.5917661639161444 1106 76-77 0.9036946410895084 1106 78-79 0.7151887341502565 1106 80-81 0.8583860261274054 1106 82-83 0.9924895242945979 1106 84-85 0.9782183306652428 1106 86-87 1.1813438712674476 1106 88-89 1.19277783991949 1106 90-91 1.1184570737533548 1106 92-93 1.054674700432912 1106 94-95 2.025315227642473 1106 96-97 2.1072158587474057 1106 98-99 1.863890743512961 1106 100-101 1.6376367367788376 1107 1 9.797880547921523E-4 1107 2 -0.03657063557770712 1107 3 0.04098364810885258 1107 4 -0.03844814188246204 1107 5 0.057648287268385445 1107 6 0.07335838655261995 1107 7 0.1592083721748665 1107 8 0.03375341111573249 1107 9 0.18230244564179543 1107 10-11 0.11984637240043838 1107 12-13 0.17102134166900385 1107 14-15 0.168440009213235 1107 16-17 0.10266090826586804 1107 18-19 0.33905517698411103 1107 20-21 0.2189012501155716 1107 22-23 0.2234149119851523 1107 24-25 0.4892430757724213 1107 26-27 0.3924741269020089 1107 28-29 0.29268288672461296 1107 30-31 0.21927397024430917 1107 32-33 0.19704243922154063 1107 34-35 0.27406051971642853 1107 36-37 0.17600457351827004 1107 38-39 0.017713841672303943 1107 40-41 0.0843709055186892 1107 42-43 0.18505772230674467 1107 44-45 0.21434310959001834 1107 46-47 0.10260246780859461 1107 48-49 0.19643412687456419 1107 50-51 0.16998591110076688 1107 52-53 0.2168015963336103 1107 54-55 0.1163453187558261 1107 56-57 0.2110989804534782 1107 58-59 0.08676499614601951 1107 60-61 0.2036539400025319 1107 62-63 0.3362996463815904 1107 64-65 0.30460470612673163 1107 66-67 1.0482882637806519 1107 68-69 -5.586574375599049 1107 70-71 -6.1245270103359175 1107 72-73 -2.5011669114256136 1107 74-75 0.14931237753392423 1107 76-77 0.24011955231600624 1107 78-79 0.3077999879724338 1107 80-81 0.2263386724485592 1107 82-83 0.20315097276069238 1107 84-85 0.17229800231913117 1107 86-87 0.2840416163379693 1107 88-89 0.18046992063613487 1107 90-91 0.18798950757202348 1107 92-93 0.33478757207124943 1107 94-95 1.1689761845055031 1107 96-97 1.3342410317006035 1107 98-99 1.1561619763181703 1107 100-101 0.9686844797987959 1108 1 0.005858707634907034 1108 2 -0.03882025144829626 1108 3 -0.05503758717558682 1108 4 0.014220699788772606 1108 5 0.06512651000588932 1108 6 0.11209909954659736 1108 7 0.16462868469763947 1108 8 0.09139883373893554 1108 9 0.17369345741253994 1108 10-11 0.27845817451126464 1108 12-13 0.2271312041164748 1108 14-15 0.27738669514692305 1108 16-17 0.10401146503675562 1108 18-19 0.1568452809934726 1108 20-21 0.209559535635087 1108 22-23 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0.556779610915239 2316 92-93 0.8330037839285822 2316 94-95 1.388258490989866 2316 96-97 1.6739024107828406 2316 98-99 0.580807297114589 2316 100-101 0.5596056698496135 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 28.0 4 89.0 5 239.0 6 521.0 7 1217.0 8 2623.0 9 4410.0 10 7177.0 11 10641.0 12 14072.0 13 17889.0 14 21724.0 15 26848.0 16 31896.0 17 36090.0 18 34003.0 19 29985.0 20 29293.0 21 29232.0 22 31367.0 23 35333.0 24 43300.0 25 53771.0 26 67528.0 27 84416.0 28 102760.0 29 122917.0 30 145133.0 31 168929.0 32 193995.0 33 225112.0 34 261883.0 35 298756.0 36 318457.0 37 268643.0 38 110775.0 39 6421.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.225893918435894 20.03619525414934 15.594168996727339 23.14374183068743 2 16.149974955294937 20.9025470165429 39.041152627473316 23.906325400688843 3 19.332605222731004 24.47540617893124 30.34015029527951 25.851838303058237 4 13.533271236001228 17.67345754914306 36.124752905834434 32.66851830902128 5 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50-51 22.35416339626036 27.152850412644437 28.232640095387097 22.260346095708112 52-53 22.70289572668262 27.087062250805445 28.005896906416588 22.204145116095344 54-55 22.3591039882752 26.982989558660925 28.196922840378534 22.460983612685336 56-57 22.37693588677571 27.387321196484983 27.98366343100031 22.252079485738996 58-59 22.465455554190672 27.017551960524965 28.083066343770703 22.433926141513663 60-61 22.465120983943528 26.995762777576488 28.328538760963134 22.210577477516853 62-63 22.39525218912581 27.014566682432083 28.309469048443063 22.28071207999904 64-65 22.201909845117832 26.925947287795427 28.239390938835367 22.632751928251373 66-67 21.818104926697966 27.145711549609196 28.62286451598291 22.41331900770993 68-69 21.87792640001819 26.636119609562286 29.20948654513211 22.27646744528742 70-71 21.45769508660244 26.668988533604132 29.418142990024148 22.45517338976928 72-73 22.231143631848134 26.93577556921843 28.250762683337765 22.58231811559567 74-75 21.95598895153201 27.19795830636275 28.092663978196715 22.75338876390853 76-77 22.168775175568083 27.243348433387048 27.84238067644293 22.745495714601933 78-79 22.12247150795694 27.308233936542898 27.798005772864787 22.771288782635374 80-81 22.035338192173228 27.47501268749295 27.731335288147065 22.75831383218676 82-83 22.230527711022557 27.452659594901252 27.60395576217074 22.71285693190545 84-85 22.197875510003414 27.351989317285298 27.48159370934995 22.96854146336134 86-87 22.173342726654703 27.24023842307797 27.675554726963426 22.910864123303906 88-89 22.071931497973456 27.309778610368028 27.54468912111116 23.073600770547355 90-91 22.016680852263615 27.429710807662687 27.63493498414914 22.918673355924557 92-93 22.09708768659018 27.16275438908733 27.648985791745005 23.091172132577487 94-95 21.653067939009084 27.121792690294622 27.93273614430022 23.29240322639608 96-97 21.542478599449947 26.99351678310365 28.031576805668102 23.432427811778304 98-99 21.719273121018254 26.949599915547225 27.565777380578716 23.765349582855805 100-101 21.67882361420755 26.862633453713286 27.78133460497983 23.67720832709934 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2293.0 1 1556.0 2 845.5 3 957.5 4 945.5 5 915.5 6 1329.5 7 2480.5 8 3749.0 9 4543.0 10 4494.0 11 3919.0 12 3749.5 13 3828.5 14 3968.0 15 4187.0 16 4407.0 17 4654.5 18 4989.5 19 5489.5 20 6118.5 21 6915.0 22 7970.0 23 9563.0 24 11551.5 25 14002.5 26 17331.0 27 21289.5 28 25195.0 29 29220.0 30 33691.5 31 38958.5 32 44269.5 33 48389.0 34 52585.5 35 57433.0 36 63514.5 37 69486.5 38 73517.0 39 76052.0 40 78802.0 41 83860.0 42 88962.0 43 92751.5 44 96767.0 45 100245.0 46 101833.0 47 104371.5 48 108534.0 49 110083.5 50 108751.5 51 106366.0 52 103606.0 53 101757.5 54 98670.5 55 91328.0 56 82250.5 57 73916.5 58 65795.5 59 56813.0 60 47560.5 61 38752.0 62 30856.5 63 23928.0 64 18045.0 65 13658.0 66 10395.0 67 8132.0 68 5934.0 69 3898.0 70 2784.0 71 2188.5 72 1761.0 73 1430.5 74 1153.0 75 876.5 76 583.5 77 344.0 78 203.5 79 114.5 80 73.5 81 49.0 82 28.5 83 24.0 84 15.5 85 8.5 86 4.0 87 4.0 88 3.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02273142974349432 2 0.019524359810691245 3 0.0 4 0.0 5 2.114551604045983E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.9956147723984763E-4 18-19 0.0010572758020229915 20-21 1.7621263367049858E-5 22-23 0.0 24-25 0.0 26-27 4.052890574421467E-4 28-29 0.01867853916907285 30-31 0.0044229371051295145 32-33 0.007929568515172437 34-35 0.00891635926372723 36-37 0.09570108134644778 38-39 0.09823854327130296 40-41 0.1122474476481076 42-43 0.09904912138618725 44-45 0.09395657627310985 46-47 0.09513720091870219 48-49 0.09774514789702557 50-51 0.09571870260981484 52-53 0.07386833603467301 54-55 0.06278456137679865 56-57 0.03603548358561696 58-59 0.01577103071350962 60-61 0.00863441904985443 62-63 0.0016563987565026868 64-65 0.004599149738800013 66-67 0.41498075229402415 68-69 0.7960934363965786 70-71 0.8841997532318279 72-73 0.27607233317157015 74-75 0.14615075836631153 76-77 0.014273223327310387 78-79 0.008511070206285082 80-81 0.0014449435960980884 82-83 0.0 84-85 0.0 86-87 1.4097010693639886E-4 88-89 6.16744217846745E-4 90-91 0.003383282566473573 92-93 0.0016211562297685869 94-95 0.22105874893964048 96-97 0.22833633071023207 98-99 0.01383269174313414 100-101 0.00755952198446439 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2837481.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.297089049740045 #Duplication Level Percentage of deduplicated Percentage of total 1 75.70813455003521 42.621475925530326 2 14.022420016756765 15.788428567524257 3 4.604912080048542 7.777294363101493 4 2.014716525185117 4.536907025133175 5 1.053059415051184 2.964208983190184 6 0.6183741028222679 2.0887597159582993 7 0.39340607675830636 1.550333185517987 8 0.27390639691332946 1.233610625465852 9 0.20210966287598411 1.0240367119867995 >10 0.9825174689987165 10.108622760563517 >50 0.07873610356044947 3.075484764892794 >100 0.04405084827780701 4.486201721794326 >500 0.0028371357282697012 1.0484893644577649 >1k 6.304746062201899E-4 0.5712030921092931 >5k 6.3047460622019E-5 0.27007297134528124 >10k+ 1.26094921244038E-4 0.8548702214287159 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12498 0.44046109912277825 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11587 0.40835515726801347 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7609 0.2681603859197647 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3388 0.11940168057512984 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.5242526734099716E-5 0.0 0.0 0.0 6 0.0 3.5242526734099716E-5 0.0 0.0 0.0 7 0.0 3.5242526734099716E-5 0.0 0.0 0.0 8 0.0 3.5242526734099716E-5 0.0 0.0 0.0 9 0.0 3.5242526734099716E-5 0.0 0.0 0.0 10-11 0.0 3.5242526734099716E-5 0.0 0.0 0.0 12-13 0.0 3.5242526734099716E-5 0.0 0.0 0.0 14-15 0.0 3.5242526734099716E-5 0.0 0.0 3.5242526734099716E-5 16-17 0.0 3.5242526734099716E-5 0.0 7.048505346819943E-5 5.286379010114957E-5 18-19 0.0 3.5242526734099716E-5 0.0 7.048505346819943E-5 1.0572758020229915E-4 20-21 0.0 3.5242526734099716E-5 0.0 7.048505346819943E-5 1.0572758020229915E-4 22-23 0.0 3.5242526734099716E-5 0.0 1.2334884356934902E-4 1.0572758020229915E-4 24-25 0.0 3.5242526734099716E-5 0.0 2.4669768713869803E-4 1.0572758020229915E-4 26-27 0.0 3.5242526734099716E-5 0.0 4.581528475432963E-4 1.0572758020229915E-4 28-29 0.0 3.5242526734099716E-5 0.0 0.0013039734891616896 1.0572758020229915E-4 30-31 0.0 3.5242526734099716E-5 0.0 0.003982405520953268 1.0572758020229915E-4 32-33 0.0 3.5242526734099716E-5 0.0 0.009057329370663628 1.0572758020229915E-4 34-35 0.0 3.5242526734099716E-5 0.0 0.016845927778899664 1.0572758020229915E-4 36-37 0.0 3.5242526734099716E-5 0.0 0.03226453322506829 1.0572758020229915E-4 38-39 0.0 3.5242526734099716E-5 0.0 0.05566557097651051 1.0572758020229915E-4 40-41 0.0 3.5242526734099716E-5 0.0 0.07947189778539487 1.0572758020229915E-4 42-43 0.0 3.5242526734099716E-5 0.0 0.1050051084042501 1.2334884356934902E-4 44-45 0.0 3.5242526734099716E-5 0.0 0.1369524588887115 1.4097010693639886E-4 46-47 0.0 3.5242526734099716E-5 0.0 0.1767765140982442 1.4097010693639886E-4 48-49 0.0 3.5242526734099716E-5 0.0 0.2221688885317646 1.4097010693639886E-4 50-51 0.0 3.5242526734099716E-5 0.0 0.2714731834327701 1.4097010693639886E-4 52-53 0.0 3.5242526734099716E-5 0.0 0.319843551375322 1.4097010693639886E-4 54-55 0.0 3.5242526734099716E-5 0.0 0.37674261078752597 1.4097010693639886E-4 56-57 0.0 3.5242526734099716E-5 0.0 0.4406196904930817 1.4097010693639886E-4 58-59 0.0 3.5242526734099716E-5 0.0 0.5034042518698804 1.4097010693639886E-4 60-61 0.0 5.286379010114957E-5 0.0 0.5683209861140921 1.4097010693639886E-4 62-63 3.5242526734099716E-5 7.048505346819943E-5 0.0 0.6351760593286793 1.4097010693639886E-4 64-65 3.5242526734099716E-5 7.048505346819943E-5 0.0 0.7039342289869077 1.4097010693639886E-4 66-67 3.5242526734099716E-5 7.048505346819943E-5 0.0 0.7798994953622597 1.762126336704986E-4 68-69 3.5242526734099716E-5 7.048505346819943E-5 0.0 0.8731159785739535 1.762126336704986E-4 70-71 3.5242526734099716E-5 7.048505346819943E-5 0.0 0.9934515861075369 1.762126336704986E-4 72-73 3.5242526734099716E-5 7.048505346819943E-5 0.0 1.1271441112733442 1.762126336704986E-4 74-75 3.5242526734099716E-5 7.048505346819943E-5 0.0 1.2700349359167515 1.762126336704986E-4 76-77 5.286379010114957E-5 7.048505346819943E-5 0.0 1.433806957650113 1.762126336704986E-4 78-79 7.048505346819943E-5 7.048505346819943E-5 0.0 1.62083904702798 1.762126336704986E-4 80-81 7.048505346819943E-5 7.048505346819943E-5 0.0 1.8359418089495576 2.114551604045983E-4 82-83 7.048505346819943E-5 7.048505346819943E-5 0.0 2.060965342146784 2.4669768713869803E-4 84-85 7.048505346819943E-5 7.048505346819943E-5 0.0 2.282764184147841 2.4669768713869803E-4 86-87 7.048505346819943E-5 7.048505346819943E-5 0.0 2.519276781060384 2.4669768713869803E-4 88-89 7.048505346819943E-5 7.048505346819943E-5 0.0 2.7772344554906274 2.4669768713869803E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6145 0.0 60.078667 1 GTATCAA 11120 0.0 41.808918 1 GTGGTAT 2550 0.0 35.869213 1 TGGTATC 2470 0.0 35.298534 2 ATCAACG 14850 0.0 31.039522 3 TATCAAC 14920 0.0 31.028555 2 TCAACGC 15110 0.0 30.505423 4 CAACGCA 15305 0.0 30.054846 5 AACGCAG 15775 0.0 29.069304 6 ACGCAGA 18440 0.0 24.816748 7 CGCAGAG 18570 0.0 24.438934 8 GCAGAGT 19570 0.0 22.512346 9 GTACATG 11875 0.0 22.189415 1 GAGTACT 12165 0.0 22.13922 12-13 TACATGG 11775 0.0 21.85117 2 ACATGGG 11940 0.0 20.988432 3 GTACTTT 12900 0.0 20.785988 14-15 CAGAGTA 19250 0.0 20.51215 10-11 AGAGTAC 18520 0.0 20.348644 10-11 AGTACTT 12590 0.0 20.056051 12-13 >>END_MODULE