FastQCFastQC Report
Fri 27 May 2016
SRR939534_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939534_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804212
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85820.30603962895815295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67850.24195745542776367No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44840.1599023183696525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51850.056.134811
GTATCAA101350.046.2377971
TATCAAC129700.035.919232
ATCAACG136050.034.6144873
GTGGTAT25550.033.958051
TGGTATC24250.033.9378242
TCAACGC140300.033.485074
CAACGCA139550.032.9615755
AACGCAG143600.031.7648566
ACGCAGA158650.028.2331287
CGCAGAG162100.027.5723618
GCAGAGT159650.025.352369
GTACATG100750.025.2342641
TACATGG101350.024.1675552
ACATGGG107000.022.6811143
GAGTACT100050.022.28151712-13
CAGAGTA156700.021.23047610-11
AGAGTAC148200.021.17101110-11
GTACTTT111000.020.41294314-15
AGTACTT106650.020.02323312-13
Produced by FastQC (version 0.11.5)