FastQCFastQC Report
Fri 27 May 2016
SRR939534_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939534_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804212
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121340.43270622905828804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110100.3926236675401147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74340.2651012120338976No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31940.11390009029274534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63100.056.1285631
GTATCAA111100.041.02341
TGGTATC27450.034.7570462
GTGGTAT30000.031.9671971
TATCAAC150300.030.4128062
ATCAACG150800.030.1779793
TCAACGC152950.029.7007314
CAACGCA155700.029.1790335
AACGCAG160200.028.3889986
ACGCAGA186350.024.3288977
CGCAGAG187750.024.0464488
GCAGAGT200100.021.8513269
GTACATG115550.021.2419831
GAGTACT123000.021.16105312-13
AGTACTT125900.020.44768312-13
TACATGG116200.020.4245762
AGAGTAC186050.020.261410-11
GTACTTT128550.020.04460514-15
ACATGGG115450.019.8118953
CAGAGTA196650.019.65149710-11
Produced by FastQC (version 0.11.5)