##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939534_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2804212 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.86966748591048 31.0 31.0 31.0 28.0 34.0 2 31.131430861860657 31.0 31.0 34.0 28.0 34.0 3 31.13108210078268 31.0 31.0 34.0 28.0 34.0 4 35.04520770897493 35.0 35.0 37.0 32.0 37.0 5 34.60722156527395 35.0 35.0 37.0 30.0 37.0 6 34.66535625694491 35.0 35.0 37.0 31.0 37.0 7 34.50989190546221 35.0 35.0 37.0 30.0 37.0 8 34.40239824949041 35.0 35.0 37.0 30.0 37.0 9 35.91286286486186 37.0 35.0 39.0 30.0 39.0 10-11 35.70579916924969 37.0 35.0 39.0 30.0 39.0 12-13 35.690110804746574 37.0 35.0 39.0 30.0 39.0 14-15 36.52624516263393 38.0 35.0 40.0 30.0 41.0 16-17 36.42970253318936 38.0 35.0 40.0 30.0 41.0 18-19 36.425021360724514 38.0 35.0 40.0 30.0 41.0 20-21 36.29867338845993 38.0 34.0 40.0 29.5 41.0 22-23 36.13989990771026 38.0 34.0 40.0 29.0 41.0 24-25 35.9702636605221 38.0 34.0 40.0 29.0 41.0 26-27 35.50257059737281 38.0 34.0 40.0 27.0 41.0 28-29 35.28599460383167 38.0 33.5 40.0 27.0 41.0 30-31 34.98790052249973 37.5 33.0 40.0 27.0 41.0 32-33 34.745379628929626 37.0 33.0 40.0 25.5 41.0 34-35 35.002555619903205 37.5 33.0 40.0 26.0 41.0 36-37 34.83637720685883 38.0 33.0 40.0 25.0 41.0 38-39 34.64827088679458 37.0 33.0 40.0 25.0 41.0 40-41 34.484840304513355 37.0 33.0 40.0 24.5 41.0 42-43 34.32913435218165 37.0 33.0 40.0 24.5 41.0 44-45 33.93570921171438 37.0 32.5 40.0 24.0 41.0 46-47 33.53091064441632 37.0 32.0 40.0 23.0 41.0 48-49 33.13269610143598 37.0 31.0 39.5 21.0 41.0 50-51 32.88583762568594 36.5 31.0 39.0 20.0 40.0 52-53 32.77554407441377 36.0 31.0 39.0 19.5 40.0 54-55 32.43124503425561 36.0 31.0 39.0 18.5 40.0 56-57 31.92047373736365 35.5 30.0 39.0 15.5 40.0 58-59 31.44606595364402 35.0 29.0 38.5 13.0 40.0 60-61 31.074735077091177 35.0 29.0 38.0 11.5 40.0 62-63 30.90898922763329 34.5 29.0 38.0 10.0 40.0 64-65 30.448852297900444 34.0 28.0 38.0 8.0 40.0 66-67 29.595136530333654 34.0 27.0 37.5 6.5 39.5 68-69 26.219671872169435 31.0 18.5 36.0 2.0 39.0 70-71 24.910882094506405 29.5 13.0 35.0 2.0 38.0 72-73 25.881821345889684 30.5 19.5 35.0 2.0 38.0 74-75 26.597406865101497 31.0 22.5 35.0 2.0 37.0 76-77 26.27463544125765 30.5 23.0 34.0 2.0 36.0 78-79 27.369325856960884 32.0 25.5 35.0 2.0 37.0 80-81 27.377634073315427 32.0 26.0 35.0 2.0 36.0 82-83 27.05131994300003 32.0 25.5 35.0 2.0 36.0 84-85 26.6898048364389 32.0 25.0 34.5 2.0 36.0 86-87 26.236512610316197 32.0 24.0 34.0 2.0 35.0 88-89 25.757447190155382 31.0 23.5 34.0 2.0 35.0 90-91 25.159601520855055 31.0 20.0 34.0 2.0 35.0 92-93 24.58995022487601 31.0 18.5 34.0 2.0 35.0 94-95 23.74676575808106 30.5 10.0 34.0 2.0 35.0 96-97 23.061620876025067 30.0 2.0 34.0 2.0 35.0 98-99 22.19798110841834 30.0 2.0 34.0 2.0 35.0 100-101 20.782132378008512 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.23813236943045624 1101 2 0.24829439915607665 1101 3 0.3037798306932835 1101 4 0.4039925783440026 1101 5 0.5705696457636122 1101 6 0.4690955922049582 1101 7 0.6512204363697123 1101 8 0.6394086452104801 1101 9 0.5404798525682395 1101 10-11 0.7599444963051241 1101 12-13 0.6932979350827608 1101 14-15 0.7670561629782213 1101 16-17 0.6510173652648632 1101 18-19 0.5976007711128872 1101 20-21 0.6433956787813955 1101 22-23 0.7547376655234643 1101 24-25 0.6893712558135121 1101 26-27 0.797739458148385 1101 28-29 0.6904886036856439 1101 30-31 0.5224891869424866 1101 32-33 0.7546078596774493 1101 34-35 0.7174115716912084 1101 36-37 0.9163230550589816 1101 38-39 0.9394176388839597 1101 40-41 0.9988342229488225 1101 42-43 0.6746010981539925 1101 44-45 0.8236293942592923 1101 46-47 0.6564545632335168 1101 48-49 0.5936408788700263 1101 50-51 0.537920935460356 1101 52-53 0.31236504245741514 1101 54-55 0.4077431139194658 1101 56-57 0.5702651251213382 1101 58-59 0.49556582751388234 1101 60-61 0.3135666700066544 1101 62-63 0.027461244382788408 1101 64-65 -0.06152703603746801 1101 66-67 -0.24752461871540632 1101 68-69 -6.9228317764305665 1101 70-71 -7.952033100129732 1101 72-73 -5.02236141275765 1101 74-75 -3.0122808099884644 1101 76-77 -0.28829421130347654 1101 78-79 -0.03623265591324909 1101 80-81 -0.1479387972157049 1101 82-83 -0.4108895702176234 1101 84-85 -0.4747919362144657 1101 86-87 -0.6158449537345554 1101 88-89 -0.7243793104758893 1101 90-91 -0.764735507487277 1101 92-93 -0.5898851929693443 1101 94-95 -0.5910650983992376 1101 96-97 -0.9805676030194412 1101 98-99 -1.0147499661121948 1101 100-101 -0.9490639501577007 1102 1 0.4017329502467213 1102 2 0.3833221799339377 1102 3 0.34312550380070306 1102 4 0.36190294269134426 1102 5 0.46424255431933403 1102 6 0.47733022277461856 1102 7 0.6445485924152266 1102 8 0.6954803866478159 1102 9 0.6696963849168611 1102 10-11 0.7359525673882601 1102 12-13 0.6861615066913274 1102 14-15 0.7804101368894862 1102 16-17 0.7321271220523329 1102 18-19 0.5672450640102298 1102 20-21 0.7969430638224608 1102 22-23 0.6630130972355559 1102 24-25 0.6227867057318264 1102 26-27 0.7800615917395533 1102 28-29 0.6362638853935323 1102 30-31 0.5142670710089519 1102 32-33 0.8136699134149552 1102 34-35 0.6528751360995884 1102 36-37 0.9152169669456569 1102 38-39 1.0081259055725695 1102 40-41 0.7739487832993674 1102 42-43 0.6417673216653981 1102 44-45 0.7416761852837297 1102 46-47 0.7569798350839818 1102 48-49 0.7758836388101642 1102 50-51 0.6169006326061606 1102 52-53 0.32514128590651836 1102 54-55 0.20535967575462166 1102 56-57 0.22627652201187942 1102 58-59 0.3035153643866302 1102 60-61 0.48100375824512653 1102 62-63 0.30867128969262936 1102 64-65 0.11204273820192867 1102 66-67 0.15992227551192073 1102 68-69 -6.79499562558977 1102 70-71 -7.6109988083124165 1102 72-73 -1.5116644846722345 1102 74-75 0.07000806552862215 1102 76-77 0.5642108588384893 1102 78-79 0.33274630406905814 1102 80-81 0.20538418154198723 1102 82-83 0.08914608792366607 1102 84-85 -0.19931770865037635 1102 86-87 -0.3430630872708669 1102 88-89 -0.3286308580906514 1102 90-91 -0.371238489570775 1102 92-93 -0.3556912641107637 1102 94-95 -0.38796746880846555 1102 96-97 -0.7652973468808391 1102 98-99 -0.6793143453433856 1102 100-101 -0.653772196274307 1103 1 0.29987405093794095 1103 2 0.24157674338859536 1103 3 0.25937733110560757 1103 4 0.23783341482736375 1103 5 0.33663596047109223 1103 6 0.3491531451390557 1103 7 0.3070846549820132 1103 8 0.3264568471810776 1103 9 0.5058082635819332 1103 10-11 0.4368567958291649 1103 12-13 0.3407627238933699 1103 14-15 0.5252217215121249 1103 16-17 0.5807444775058599 1103 18-19 0.6108236743374391 1103 20-21 0.5424849607968909 1103 22-23 0.31737451617183154 1103 24-25 0.5483893907630417 1103 26-27 0.7189371966801446 1103 28-29 0.4987445954047729 1103 30-31 0.5391830363197272 1103 32-33 0.8051062834134228 1103 34-35 0.4974411367067546 1103 36-37 0.8583801781786136 1103 38-39 0.9585050661445251 1103 40-41 0.8903399452009353 1103 42-43 0.6102036186215472 1103 44-45 0.823353846537394 1103 46-47 0.6674648660083733 1103 48-49 0.8424164351993184 1103 50-51 0.5937691609763363 1103 52-53 0.47806912463970974 1103 54-55 0.24021892852615423 1103 56-57 0.6094499325683458 1103 58-59 0.6770092822003839 1103 60-61 0.5533583821398089 1103 62-63 0.1843910585685542 1103 64-65 0.2554781789336289 1103 66-67 0.08955137681319059 1103 68-69 -6.580522317791324 1103 70-71 -7.576645642382324 1103 72-73 -1.0229646523592244 1103 74-75 0.6121299304863435 1103 76-77 0.6985568535424242 1103 78-79 0.40487315563365556 1103 80-81 0.29902648499303197 1103 82-83 0.18716937878050643 1103 84-85 -0.12485837975522429 1103 86-87 -0.04697645974557574 1103 88-89 0.11287194273314327 1103 90-91 -0.13070697424856803 1103 92-93 0.032269743483087865 1103 94-95 0.2504856877336401 1103 96-97 0.21438924060972653 1103 98-99 0.3501821159834755 1103 100-101 0.4978246429329509 1104 1 0.23016972877970687 1104 2 0.18216198791700577 1104 3 0.14290377509020757 1104 4 0.14566130915434883 1104 5 0.3456017638392339 1104 6 0.2452041474475024 1104 7 0.29794109002622093 1104 8 0.4697807305020021 1104 9 0.5849175082734774 1104 10-11 0.4228011390497457 1104 12-13 0.27714174490851917 1104 14-15 0.6166220322799489 1104 16-17 0.5369560332261614 1104 18-19 0.5077554773054302 1104 20-21 0.49618265805299444 1104 22-23 0.48974757891750187 1104 24-25 0.5912816070261897 1104 26-27 0.6264124504521646 1104 28-29 0.4891999725486684 1104 30-31 0.4123917813784388 1104 32-33 0.6360623268696699 1104 34-35 0.31462508781410037 1104 36-37 0.581323049554598 1104 38-39 0.6482376422526386 1104 40-41 0.5839617099667365 1104 42-43 0.3333640210695137 1104 44-45 0.6450102898664696 1104 46-47 0.5800874120710446 1104 48-49 0.7179293547833936 1104 50-51 0.5896563023329975 1104 52-53 0.3550252227589894 1104 54-55 0.4152726146608998 1104 56-57 0.43003089160118435 1104 58-59 0.47836732734819876 1104 60-61 0.49668665344946916 1104 62-63 0.1117645601131052 1104 64-65 0.25960027263843344 1104 66-67 0.2527508206169351 1104 68-69 -6.673524778267929 1104 70-71 -4.534119305374968 1104 72-73 0.16483544710185427 1104 74-75 0.8754184899640904 1104 76-77 0.9439403370673922 1104 78-79 0.5544503499643305 1104 80-81 0.330917821309626 1104 82-83 0.4301191300103717 1104 84-85 0.26701154189515464 1104 86-87 0.1545760875406117 1104 88-89 0.2405702088263304 1104 90-91 0.1847719603093303 1104 92-93 0.18580546950518695 1104 94-95 0.24990094285941566 1104 96-97 0.14025283629638707 1104 98-99 0.3384413101010537 1104 100-101 0.4901144876323116 1105 1 0.08054040848932686 1105 2 0.2478170567707565 1105 3 0.1825863214549024 1105 4 0.1857607267974828 1105 5 0.35901489453727464 1105 6 0.3063679308158811 1105 7 0.2613398759741301 1105 8 0.41832949012840004 1105 9 0.31396096497306303 1105 10-11 0.44060588921428945 1105 12-13 0.38278293629527127 1105 14-15 0.5004537623620564 1105 16-17 0.4581765704459997 1105 18-19 0.47244592222842385 1105 20-21 0.5467666356722347 1105 22-23 0.4865333761204198 1105 24-25 0.524412128255257 1105 26-27 0.5982998975223452 1105 28-29 0.49071090728963185 1105 30-31 0.4619902546676258 1105 32-33 0.43680632791200935 1105 34-35 0.5795077397595207 1105 36-37 0.6478329623768317 1105 38-39 0.6116134850198662 1105 40-41 0.6833771429813851 1105 42-43 0.5537108546053702 1105 44-45 0.698954022663024 1105 46-47 0.7584779037434117 1105 48-49 0.8335540923312976 1105 50-51 0.7307284476908791 1105 52-53 0.6540528528736544 1105 54-55 0.8000648536353907 1105 56-57 0.7099268032316317 1105 58-59 0.7729866568305859 1105 60-61 0.8498141457577155 1105 62-63 0.5604689229167761 1105 64-65 0.6621251696730184 1105 66-67 0.7372825913442576 1105 68-69 -3.139314759338749 1105 70-71 -3.2727413295234022 1105 72-73 0.8773263987534143 1105 74-75 1.2067098626756412 1105 76-77 1.0815635330019475 1105 78-79 0.8381527247098255 1105 80-81 0.6806900313083482 1105 82-83 0.4866279209321114 1105 84-85 0.5687414156213606 1105 86-87 0.7073604003828784 1105 88-89 0.5662126652453381 1105 90-91 0.5635118303162194 1105 92-93 0.5680981120902118 1105 94-95 0.5491593729522855 1105 96-97 0.5327760325887674 1105 98-99 0.6923341890218886 1105 100-101 0.94092893897351 1106 1 0.12923481702245 1106 2 0.17808219468223996 1106 3 0.1411403698421161 1106 4 0.16595965549293368 1106 5 0.2293208349646676 1106 6 0.2616974864996777 1106 7 0.17900183626726118 1106 8 0.34565358625494014 1106 9 0.2922374307450397 1106 10-11 0.3500280563905491 1106 12-13 0.22673483147313078 1106 14-15 0.2799705538078996 1106 16-17 0.335683213628144 1106 18-19 0.24386141293772567 1106 20-21 0.3341132022272362 1106 22-23 0.29867850595874756 1106 24-25 0.40705830688318656 1106 26-27 0.5250557667796016 1106 28-29 0.4446246394160056 1106 30-31 0.43008904102280354 1106 32-33 0.4047348741119663 1106 34-35 0.28239838791446203 1106 36-37 0.38368394902770575 1106 38-39 0.5678952619454805 1106 40-41 0.2715831679140379 1106 42-43 0.3394154940842071 1106 44-45 0.39479284869708664 1106 46-47 0.4640707168527882 1106 48-49 0.6939968630184481 1106 50-51 0.5231389168456104 1106 52-53 0.30130968611844366 1106 54-55 0.14073437869715377 1106 56-57 0.6352293770168558 1106 58-59 0.7470589539872172 1106 60-61 0.6851961027605249 1106 62-63 0.2332992513594334 1106 64-65 0.31534003734150673 1106 66-67 0.6726465597079958 1106 68-69 -2.9449639446240674 1106 70-71 -3.449829834899642 1106 72-73 0.8096525449699961 1106 74-75 1.3620236875754763 1106 76-77 1.4626040374385774 1106 78-79 1.010268542712712 1106 80-81 0.9088472441696034 1106 82-83 0.6235272943720211 1106 84-85 0.3329232897725447 1106 86-87 0.48496813823254215 1106 88-89 0.5226545060777852 1106 90-91 0.6264578627029742 1106 92-93 0.6006989958125821 1106 94-95 0.6305159347032436 1106 96-97 0.7117804287280727 1106 98-99 0.6836069960085638 1106 100-101 0.7913964951856194 1107 1 0.007808672660527094 1107 2 0.011925212011497877 1107 3 0.010879702884199816 1107 4 -0.19666837227270406 1107 5 0.18921355861886013 1107 6 0.07977670595504094 1107 7 -3.2198296482022215E-4 1107 8 0.05834957188670131 1107 9 0.1917811422153619 1107 10-11 0.0036006984952976495 1107 12-13 0.13766148551905388 1107 14-15 0.03410263477484676 1107 16-17 -0.03665914839311313 1107 18-19 0.16344055958109038 1107 20-21 0.22535027809733066 1107 22-23 0.20706280769167051 1107 24-25 0.02026276297755203 1107 26-27 0.4223362386013605 1107 28-29 0.3245758553763949 1107 30-31 0.29761367574927533 1107 32-33 0.19675346544248384 1107 34-35 0.12607102516508206 1107 36-37 0.09337037201688503 1107 38-39 0.09728703904367819 1107 40-41 -0.03199432832400362 1107 42-43 -0.0012597158570599731 1107 44-45 0.22656072404294747 1107 46-47 0.4446602513414035 1107 48-49 0.37167634459701304 1107 50-51 0.3421282862937005 1107 52-53 0.12168855122208555 1107 54-55 -0.04638032447259377 1107 56-57 0.3054334933218854 1107 58-59 0.6220771569870713 1107 60-61 0.6941095050225492 1107 62-63 0.37393562823434223 1107 64-65 0.3515276010520054 1107 66-67 0.4624989940558457 1107 68-69 -2.502867687160837 1107 70-71 -2.710308695149145 1107 72-73 0.9240037900551847 1107 74-75 1.2918935968031846 1107 76-77 1.0207322836402177 1107 78-79 0.6988698242039 1107 80-81 0.5687181242239667 1107 82-83 0.6656611713682814 1107 84-85 0.4122762338043877 1107 86-87 0.331856638790768 1107 88-89 0.45243946797284096 1107 90-91 0.49323617443541323 1107 92-93 0.43665356112494536 1107 94-95 0.560567873929493 1107 96-97 0.5390278924434249 1107 98-99 0.6002857980084677 1107 100-101 0.8634682392757149 1108 1 -0.008930792256762743 1108 2 0.07710667887558387 1108 3 0.10379742212219867 1108 4 0.056727171607050764 1108 5 -0.08867659533706984 1108 6 -0.1516082792062079 1108 7 0.052156503365992535 1108 8 0.19129732755043705 1108 9 0.1148476746873186 1108 10-11 0.27478151340771007 1108 12-13 0.05773065942562994 1108 14-15 -8.304780938317435E-5 1108 16-17 0.2506305793598784 1108 18-19 0.21669277146696686 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92-93 -0.9302593507147172 2315 94-95 0.09019833437431046 2315 96-97 0.09146901756996328 2315 98-99 -2.2142168274379372 2315 100-101 -3.950964400552241 2316 1 -0.46076616995631525 2316 2 -0.36155533162762765 2316 3 -0.8221836139714327 2316 4 -0.3290056051497956 2316 5 -0.08252715653354414 2316 6 -0.48141661513159306 2316 7 -0.6651232300095131 2316 8 -0.8173247254605016 2316 9 -4.799159098303122 2316 10-11 -1.2599469133688856 2316 12-13 -0.46243198630075 2316 14-15 -0.3545936721138361 2316 16-17 -0.2424355818905113 2316 18-19 -0.6835098609305206 2316 20-21 -3.838266942973412 2316 22-23 -1.1007997150923643 2316 24-25 -0.4091585002175506 2316 26-27 -0.4512438317725227 2316 28-29 0.20700577159541922 2316 30-31 -0.031914284866111586 2316 32-33 -1.2634758596264177 2316 34-35 -0.4103620080705994 2316 36-37 -0.13442051234584795 2316 38-39 -5.567530275097212 2316 40-41 -4.88850794399788 2316 42-43 -3.4677905411761785 2316 44-45 -2.076277483868843 2316 46-47 -1.9749104685775656 2316 48-49 -2.1932144170012045 2316 50-51 -0.7449388616717556 2316 52-53 -0.5849814657316799 2316 54-55 0.4123540911879431 2316 56-57 -3.2330608888476817 2316 58-59 -0.48244689099285054 2316 60-61 -0.12482339580835955 2316 62-63 0.26492216579296723 2316 64-65 0.46139998367740276 2316 66-67 0.23659532403353367 2316 68-69 2.909273637232051 2316 70-71 3.7929316513526636 2316 72-73 2.660688184084652 2316 74-75 1.2093633207407173 2316 76-77 -1.0855797494841788 2316 78-79 0.4287165530323769 2316 80-81 0.5351065028337807 2316 82-83 0.747615486663026 2316 84-85 0.9626909136555923 2316 86-87 1.1105875821732987 2316 88-89 0.791652986589547 2316 90-91 0.6993207093792684 2316 92-93 0.573989743690003 2316 94-95 0.768890644634773 2316 96-97 1.0854993647190518 2316 98-99 1.2473348377614393 2316 100-101 1.3418503332706067 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 46.0 4 92.0 5 205.0 6 568.0 7 1377.0 8 3037.0 9 4909.0 10 7876.0 11 11371.0 12 15046.0 13 19011.0 14 23422.0 15 28651.0 16 33583.0 17 37021.0 18 35174.0 19 32011.0 20 32281.0 21 32424.0 22 34757.0 23 38603.0 24 45957.0 25 56784.0 26 70858.0 27 87892.0 28 106683.0 29 127699.0 30 149258.0 31 171906.0 32 197437.0 33 227765.0 34 260470.0 35 288082.0 36 293068.0 37 231850.0 38 92035.0 39 4991.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.99397890100766 20.211788884525514 15.706005155438946 23.088227059027886 2 16.18188458676893 21.03195238920668 38.940320301426596 23.845842722597794 3 19.30512628766993 24.54902355151732 30.423940149625935 25.72191001118682 4 13.435971057646626 17.78974567465588 36.21056629338774 32.563716974309756 5 13.742113649039373 37.485432627775644 33.961234029381515 14.811219693803466 6 31.99850795874206 36.47563023052465 16.96943740344881 14.55642440728447 7 28.195336158607127 33.05124576886483 20.267440550143856 18.485977522384182 8 26.34048352977592 35.48922121437323 19.0756975578166 19.094597698034242 9 25.9801684038154 17.00787957543866 20.10696766150348 36.904984359242455 10-11 25.112737842609267 26.95279704691763 26.77138577313337 21.16307933733973 12-13 25.875058661755958 24.181106849268172 27.600570142343017 22.34326434663285 14-15 22.69502091853255 25.647187159886624 26.126323544724865 25.53146837685596 16-17 22.097272496611772 28.823571260838005 26.777333027363113 22.301823215187106 18-19 21.84583180289636 26.839091936766508 29.692337592405703 21.622738667931433 20-21 22.654991847977257 26.09093035761918 29.783536337480903 21.470541456922657 22-23 22.300852040628925 26.113335346973066 29.67824438192731 21.907568230470694 24-25 22.120605652979872 26.3138196235959 29.79755815919039 21.768016564233836 26-27 21.951685291081144 26.552673481651073 29.556651563862395 21.93898966340539 28-29 21.84729522100168 26.613491202672584 29.78371004239927 21.75550353392646 30-31 22.004977165554678 26.35260634336309 29.67513269796643 21.967283793115808 32-33 21.93221227482397 26.40003637407527 29.556913838017685 22.11083751308308 34-35 22.33501786824253 26.554676180229425 29.35905057420518 21.751255377322863 36-37 22.29814434939803 26.346669474337915 29.368568417802898 21.986617758461158 38-39 22.056798056293317 26.571439841096662 29.49280042863494 21.87896167397508 40-41 22.26913631223728 26.379807487272256 29.447688003972804 21.903368196517654 42-43 22.094010108659884 26.525417619275693 29.191503500771248 22.189068771293176 44-45 22.27632463421985 26.70560446225638 28.978113951492695 22.039956952031066 46-47 22.348543518154365 26.878604980824587 28.51906085096998 22.25379065005107 48-49 22.379294655044742 26.954187372309008 28.469599507525405 22.196918465120845 50-51 22.348007203675852 27.162989678930387 28.25938586040407 22.22961725698969 52-53 22.596351974744056 27.097867852299256 28.084606555113396 22.22117361784329 54-55 22.352186289149692 27.006935466924304 28.18533514169106 22.45554310223494 56-57 22.388539649638332 27.370999807560224 28.022387933259246 22.218072609542194 58-59 22.494745143471047 26.98221249345038 28.092411284503445 22.430631078575132 60-61 22.408935263103647 26.982421000785706 28.404462139117655 22.204181596993 62-63 22.361307322678087 27.04809660366916 28.320938349240958 22.269657724411797 64-65 22.179809196662386 26.927924326302165 28.266011991987273 22.626254485048175 66-67 22.049676297822074 27.2210183649982 28.240704778792352 22.488600558387372 68-69 22.170137495418942 26.65900658468978 28.90463521148991 22.266220708401367 70-71 21.73656729744997 26.586056753412183 29.2251639753034 22.452211973834448 72-73 22.146050745305118 26.948879662585444 28.321827094365716 22.583242497743722 74-75 22.179034903769566 27.1670334159597 27.875223773560336 22.778707906710398 76-77 21.558624841565948 27.334994118177736 28.32887470066125 22.777506339595067 78-79 22.149803268678774 27.290924822670753 27.80436646522749 22.75490544342298 80-81 22.035426317610096 27.487331186499915 27.79006322816702 22.687179267722964 82-83 22.17474534372357 27.46748416288302 27.60253294020481 22.755237553188607 84-85 22.133724316959913 27.368639896555518 27.529112928260176 22.968522858224393 86-87 22.13105794915512 27.239046750778712 27.68468878252694 22.945206517539233 88-89 22.062053973248734 27.309799857642314 27.523675167712476 23.104471001396476 90-91 22.007274716570816 27.426508833240522 27.602305270986673 22.963911179201993 92-93 22.07425003378989 27.13269495697896 27.654574937421835 23.138480071809315 94-95 21.86807510271085 27.08886440383097 27.706101983735778 23.336958509722404 96-97 21.88580336646767 26.964114674652677 27.730479249866676 23.41960270901298 98-99 21.74645042109537 26.945645732774786 27.53272011009272 23.77518373603712 100-101 21.666332305283532 26.83928630339863 27.810664077372117 23.683717313945724 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2276.0 1 1576.5 2 905.0 3 946.5 4 899.5 5 860.0 6 1262.0 7 2420.0 8 3772.5 9 4522.0 10 4295.0 11 3734.0 12 3547.0 13 3595.5 14 3822.0 15 4148.5 16 4392.0 17 4628.5 18 5023.0 19 5483.5 20 6032.0 21 6873.0 22 8121.0 23 9686.0 24 11631.5 25 14063.5 26 17360.0 27 21422.0 28 25186.0 29 29040.0 30 33393.5 31 38596.0 32 43663.5 33 47614.5 34 51896.0 35 56680.5 36 62588.0 37 68658.0 38 72758.5 39 75265.5 40 78117.0 41 82910.5 42 87846.5 43 91386.5 44 94895.0 45 98541.0 46 100722.0 47 102492.0 48 106776.5 49 109283.0 50 107354.0 51 104703.5 52 102099.5 53 100547.5 54 97716.0 55 90452.0 56 81408.0 57 73030.0 58 65126.0 59 56210.5 60 46815.0 61 38215.0 62 30668.5 63 23841.5 64 17943.5 65 13513.5 66 10260.0 67 8056.5 68 5985.0 69 4035.5 70 2835.5 71 2170.5 72 1756.5 73 1443.0 74 1156.5 75 903.5 76 596.5 77 341.0 78 201.0 79 127.0 80 84.0 81 51.5 82 37.0 83 26.5 84 14.0 85 7.5 86 4.5 87 2.0 88 1.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020362226536367434 2 0.017045786837799712 3 6.775521964815784E-4 4 0.09425107659477956 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.012909152375070072 12-13 0.0 14-15 0.0 16-17 6.240612336014538E-4 18-19 7.132128384016615E-5 20-21 0.0 22-23 1.0698192576024922E-4 24-25 0.0034947429081681417 26-27 0.0034590822662480584 28-29 0.0013016134300830322 30-31 1.9613353056045692E-4 32-33 6.062309126414122E-4 34-35 0.0026745481440062307 36-37 0.08431958782003643 38-39 0.09844120202038933 40-41 0.07715179879409974 42-43 0.08052172945554759 44-45 0.07922011602546454 46-47 0.0779541632373016 48-49 0.07264072759120922 50-51 0.07244459406064876 52-53 0.05509569176652835 54-55 0.046376664817068036 56-57 0.026299723416061266 58-59 0.021931294780851093 60-61 0.012659527881629492 62-63 0.0019078443427244446 64-65 0.006169291052174371 66-67 0.14922195611458763 68-69 0.7006068014829121 70-71 0.8555522906256731 72-73 0.348975041829933 74-75 0.05115519083435917 76-77 0.19023169432268316 78-79 0.013836329064992233 80-81 0.0029241726374468124 82-83 0.001426425676803323 84-85 8.915160480020769E-5 86-87 0.0019791656265646107 88-89 2.852851353606646E-4 90-91 0.004154464783689678 92-93 0.0042436163884898865 94-95 0.07308648561521025 96-97 0.03484044715592116 98-99 0.014032462595552691 100-101 0.012820000770269867 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2804212.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.270402905975985 #Duplication Level Percentage of deduplicated Percentage of total 1 76.28444310325109 43.688407919811915 2 13.739571386090896 15.73741578073689 3 4.538069705159904 7.79691241289734 4 1.9372559470088666 4.437897144687835 5 1.0191497798380502 2.918355925643093 6 0.5972500590384319 2.052285091004936 7 0.3822627777974447 1.5324640310292061 8 0.25742743501233595 1.1794378337766744 9 0.1871050044885177 0.9644021093503667 >10 0.9383261562354948 9.809961458744274 >50 0.07384590606311218 2.9391080690868794 >100 0.041834692739327245 4.310702499184455 >500 0.0027664378212181487 1.0425383647630597 >1k 5.029886947311658E-4 0.4890600652577829 >5k 6.287358684139573E-5 0.2676831486619318 >10k+ 1.2574717368279146E-4 0.8333681453634316 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12134 0.43270622905828804 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11010 0.3926236675401147 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7434 0.2651012120338976 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3194 0.11390009029274534 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.5660641920083075E-5 6 0.0 0.0 0.0 0.0 3.5660641920083075E-5 7 0.0 0.0 0.0 0.0 3.5660641920083075E-5 8 0.0 0.0 0.0 0.0 3.5660641920083075E-5 9 0.0 0.0 0.0 0.0 3.5660641920083075E-5 10-11 0.0 0.0 0.0 0.0 3.5660641920083075E-5 12-13 0.0 0.0 0.0 0.0 3.5660641920083075E-5 14-15 0.0 0.0 0.0 0.0 3.5660641920083075E-5 16-17 0.0 0.0 0.0 1.7830320960041538E-5 3.5660641920083075E-5 18-19 0.0 0.0 0.0 3.5660641920083075E-5 7.132128384016615E-5 20-21 0.0 0.0 0.0 5.349096288012461E-5 7.132128384016615E-5 22-23 0.0 0.0 0.0 7.132128384016615E-5 7.132128384016615E-5 24-25 0.0 0.0 0.0 2.3179417248053998E-4 7.132128384016615E-5 26-27 0.0 0.0 0.0 4.6358834496108E-4 7.132128384016615E-5 28-29 0.0 0.0 0.0 0.0014442559977633647 7.132128384016615E-5 30-31 0.0 0.0 0.0 0.004243616388489886 7.132128384016615E-5 32-33 0.0 0.0 0.0 0.01055555000834459 7.132128384016615E-5 34-35 0.0 0.0 0.0 0.0194885408093254 7.132128384016615E-5 36-37 0.0 0.0 0.0 0.03615989090696424 7.132128384016615E-5 38-39 0.0 0.0 0.0 0.05819816761357558 7.132128384016615E-5 40-41 0.0 0.0 0.0 0.08152022742930991 7.132128384016615E-5 42-43 0.0 0.0 0.0 0.1061973916380074 7.132128384016615E-5 44-45 0.0 0.0 0.0 0.13930829766080455 7.132128384016615E-5 46-47 0.0 0.0 0.0 0.1801575629802597 7.132128384016615E-5 48-49 0.0 0.0 0.0 0.22494732923188404 7.132128384016615E-5 50-51 0.0 0.0 0.0 0.27266126812095515 7.132128384016615E-5 52-53 0.0 0.0 0.0 0.32007209155370564 8.915160480020768E-5 54-55 0.0 0.0 0.0 0.3763267541826367 1.0698192576024922E-4 56-57 0.0 0.0 0.0 0.44324394874567263 1.0698192576024922E-4 58-59 0.0 0.0 0.0 0.5136380558959166 1.0698192576024922E-4 60-61 0.0 0.0 0.0 0.5835864050221595 1.0698192576024922E-4 62-63 0.0 0.0 0.0 0.6506105815109557 1.0698192576024922E-4 64-65 0.0 1.7830320960041538E-5 0.0 0.7258188753204109 1.0698192576024922E-4 66-67 0.0 3.5660641920083075E-5 0.0 0.8094430806230057 1.0698192576024922E-4 68-69 0.0 3.5660641920083075E-5 0.0 0.9067074814600322 1.0698192576024922E-4 70-71 0.0 3.5660641920083075E-5 0.0 1.0266163899163117 1.2481224672029076E-4 72-73 0.0 3.5660641920083075E-5 0.0 1.159363129463821 2.1396385152049844E-4 74-75 0.0 3.5660641920083075E-5 0.0 1.3026832493406348 2.1396385152049844E-4 76-77 0.0 3.5660641920083075E-5 0.0 1.4673462634066183 2.1396385152049844E-4 78-79 0.0 3.5660641920083075E-5 0.0 1.6618394044387514 2.1396385152049844E-4 80-81 0.0 3.5660641920083075E-5 0.0 1.8717379427803604 2.3179417248053998E-4 82-83 1.7830320960041538E-5 3.5660641920083075E-5 0.0 2.096560459765524 2.496244934405815E-4 84-85 3.5660641920083075E-5 3.5660641920083075E-5 0.0 2.3199565510738847 2.496244934405815E-4 86-87 3.5660641920083075E-5 3.5660641920083075E-5 0.0 2.5560834915477146 2.496244934405815E-4 88-89 3.5660641920083075E-5 3.5660641920083075E-5 0.0 2.8234313240225775 2.496244934405815E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6310 0.0 56.128563 1 GTATCAA 11110 0.0 41.0234 1 TGGTATC 2745 0.0 34.757046 2 GTGGTAT 3000 0.0 31.967197 1 TATCAAC 15030 0.0 30.412806 2 ATCAACG 15080 0.0 30.177979 3 TCAACGC 15295 0.0 29.700731 4 CAACGCA 15570 0.0 29.179033 5 AACGCAG 16020 0.0 28.388998 6 ACGCAGA 18635 0.0 24.328897 7 CGCAGAG 18775 0.0 24.046448 8 GCAGAGT 20010 0.0 21.851326 9 GTACATG 11555 0.0 21.241983 1 GAGTACT 12300 0.0 21.161053 12-13 AGTACTT 12590 0.0 20.447683 12-13 TACATGG 11620 0.0 20.424576 2 AGAGTAC 18605 0.0 20.2614 10-11 GTACTTT 12855 0.0 20.044605 14-15 ACATGGG 11545 0.0 19.811895 3 CAGAGTA 19665 0.0 19.651497 10-11 >>END_MODULE