FastQCFastQC Report
Fri 27 May 2016
SRR939533_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939533_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112520
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149780.7090110389487436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115310.5458409861208414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80890.3829076174426751No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63760.3018196277431693No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27540.13036562967451196No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21990.10409368905383144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106700.038.9978681
GTATCAA172000.037.017111
TATCAAC221700.028.7345582
ATCAACG224450.028.233873
TCAACGC228050.027.7240454
CAACGCA228950.027.3691125
AACGCAG233700.026.9077286
GTACATG144900.026.3641511
TACATGG148400.025.6775322
ACATGGG152300.023.9835593
ACGCAGA256750.023.8051417
CGCAGAG258200.023.7554428
GAGTACT133700.023.30755412-13
CATGGGA104250.022.4210784
GCAGAGT254650.022.339789
AGTACTT140050.022.0284312-13
GTACTTT148550.021.11748114-15
AGAGTAC227000.020.58110410-11
ATGGGAT44000.020.438865
ATGGGAG39550.020.1712975
Produced by FastQC (version 0.11.5)