FastQCFastQC Report
Fri 27 May 2016
SRR939533_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939533_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112520
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208580.9873515990381156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185050.8759680381724196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132610.6277337019294492No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98980.46853994281710937No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45610.2159032813890519No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40610.1922348664154659No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30860.1460814572169731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120600.041.587231
GTATCAA188000.033.914551
TATCAAC243000.026.2907182
ATCAACG242950.026.2484113
TCAACGC246250.025.8966584
CAACGCA250550.025.4143925
AACGCAG255800.024.9113126
GTACATG163900.024.1217461
TACATGG166250.023.3760432
GAGTACT160400.023.02594612-13
ACATGGG167650.022.2970963
ACGCAGA289400.021.9699387
CGCAGAG291000.021.8165828
GTACTTT171150.021.63504614-15
AGTACTT164350.021.60765612-13
GCAGAGT295650.020.8324499
CATGGGA114750.020.2310434
ACTTTTT190650.019.85685716-17
TATAACG1200.00482706119.7472932
AGAGTAC266700.019.63906910-11
Produced by FastQC (version 0.11.5)