FastQCFastQC Report
Fri 27 May 2016
SRR939532_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939532_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093032
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147090.7027603973565621No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106780.5101689797384846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81660.3901517033662171No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63600.3038653971845629No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26530.12675391489475554No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21310.10181401908809803No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21130.10095402268097192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93700.042.0224651
GTATCAA160400.040.703851
TATCAAC206700.031.149172
ATCAACG213100.030.526083
TCAACGC217600.029.8061144
CAACGCA218050.029.722265
AACGCAG225300.028.7640236
ACGCAGA248050.025.5571357
CGCAGAG248500.025.316148
GTACATG144050.024.4643691
GAGTACT123950.024.37068212-13
GCAGAGT241250.024.2867589
TACATGG145000.023.6707082
AGTACTT127850.022.46095312-13
GTACTTT137650.022.1127814-15
CGCATAG3151.0913936E-1121.5742828
GTATCGA1904.9305618E-621.2278731
ACATGGG155900.021.0638333
AGAGTAC217700.020.9375610-11
ACCGTCC2359.010364E-820.6562298
Produced by FastQC (version 0.11.5)