FastQCFastQC Report
Fri 27 May 2016
SRR939532_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939532_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093032
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204720.978102580371442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174790.8351042888976374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129700.6196751889125441No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98510.4706569225888567No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43990.21017356638598933No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39000.18633255487732628No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30520.14581716858605123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115850.040.2704731
GTATCAA181200.034.1807481
TATCAAC233400.026.4269542
ATCAACG235250.026.2153723
TCAACGC238600.025.851634
CAACGCA243300.025.3718245
AACGCAG248450.024.9222356
GTACATG161050.023.7816521
TACATGG161350.023.1719132
ATAACGC1852.5750523E-723.0787933
GAGTACT149500.022.71933212-13
GTGTACG2154.465437E-822.0744951
ACGCAGA281400.022.0040137
ACATGGG162950.021.9512523
CGCAGAG282300.021.9002728
GTACTTT160400.021.1163314-15
AGTACTT153600.021.06357212-13
CATGGGG49050.020.7976594
TAACGCA1605.034343E-520.7583624
GCAGAGT287150.020.7378469
Produced by FastQC (version 0.11.5)