##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939532_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2093032 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.871495992416744 31.0 31.0 31.0 28.0 34.0 2 31.112910361618933 31.0 31.0 34.0 28.0 34.0 3 31.088184509362495 31.0 31.0 34.0 27.0 34.0 4 35.01514549228106 35.0 35.0 37.0 32.0 37.0 5 34.59068757668301 35.0 35.0 37.0 30.0 37.0 6 34.67035812161496 35.0 35.0 37.0 31.0 37.0 7 34.50276297734578 35.0 35.0 37.0 30.0 37.0 8 34.405674638514846 35.0 35.0 37.0 30.0 37.0 9 35.87238513314656 37.0 35.0 39.0 30.0 39.0 10-11 35.6773575368174 37.0 35.0 39.0 30.0 39.0 12-13 35.65188444323832 37.0 35.0 39.0 30.0 39.0 14-15 36.392876219761575 38.0 35.0 40.0 30.0 41.0 16-17 36.33664392135428 38.0 34.5 40.0 30.0 41.0 18-19 36.41602350083515 38.0 35.0 40.0 30.0 41.0 20-21 36.33770482247763 38.0 34.5 40.0 29.5 41.0 22-23 36.16930940377405 38.0 34.0 40.0 29.0 41.0 24-25 35.99329202802441 38.0 34.0 40.0 29.0 41.0 26-27 35.50331600281314 38.0 34.0 40.0 27.0 41.0 28-29 35.283908463893525 38.0 33.5 40.0 27.0 41.0 30-31 34.92764324673488 37.0 33.0 40.0 27.0 41.0 32-33 34.5837841944127 37.0 33.0 40.0 25.5 41.0 34-35 34.73696579889844 37.0 33.0 40.0 26.0 41.0 36-37 34.51472409404156 37.0 33.0 40.0 25.0 41.0 38-39 34.2986870243742 37.0 33.0 40.0 24.0 41.0 40-41 34.09711796092941 37.0 33.0 40.0 24.0 41.0 42-43 33.86608327058545 37.0 33.0 40.0 24.0 41.0 44-45 33.3707122012468 37.0 32.0 40.0 21.5 41.0 46-47 32.8454096736218 36.5 31.0 40.0 19.0 41.0 48-49 32.36181219398461 36.0 31.0 39.0 15.5 40.5 50-51 32.09372145289704 36.0 30.0 39.0 14.0 40.0 52-53 31.97067364474122 36.0 30.0 39.0 13.5 40.0 54-55 31.636982616605955 35.5 30.0 39.0 10.5 40.0 56-57 31.144330569241177 35.0 29.0 39.0 8.0 40.0 58-59 30.691571605211962 35.0 28.0 38.0 7.5 40.0 60-61 30.32821882321914 34.5 28.0 38.0 7.0 40.0 62-63 30.171681560530367 34.0 28.0 38.0 4.0 40.0 64-65 29.731225322880874 34.0 28.0 38.0 2.0 40.0 66-67 28.89553098089279 33.5 26.5 37.0 2.0 39.5 68-69 25.59158388404955 31.0 15.5 35.5 2.0 39.0 70-71 24.334751451482823 29.5 10.5 35.0 2.0 38.0 72-73 25.287519971027677 30.0 16.5 35.0 2.0 38.0 74-75 25.987425180312577 31.0 21.0 35.0 2.0 37.0 76-77 25.646266516708774 30.5 21.0 34.0 2.0 36.0 78-79 26.71349649694797 32.0 24.5 35.0 2.0 37.0 80-81 26.718994979532084 32.0 25.0 35.0 2.0 36.0 82-83 26.39659857087708 32.0 24.0 35.0 2.0 36.0 84-85 26.028774046455094 32.0 23.5 34.5 2.0 36.0 86-87 25.581321260257845 31.5 21.5 34.0 2.0 35.0 88-89 25.10202662931097 31.0 20.0 34.0 2.0 35.0 90-91 24.506174296427382 31.0 18.0 34.0 2.0 35.0 92-93 23.94490528572903 31.0 12.5 34.0 2.0 35.0 94-95 23.117200310363145 30.0 4.5 34.0 2.0 35.0 96-97 22.454846605307516 30.0 2.0 34.0 2.0 35.0 98-99 21.619923871206936 29.0 2.0 34.0 2.0 35.0 100-101 20.259891392009294 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.28161934828108315 1101 2 0.18168368042339011 1101 3 0.23605783638864608 1101 4 0.29318760672703803 1101 5 0.42994503779697624 1101 6 0.3478210644748714 1101 7 0.3957581185350918 1101 8 0.53534823012847 1101 9 0.5178986325180759 1101 10-11 0.6355698582843488 1101 12-13 0.5265324061499825 1101 14-15 0.5053678521723199 1101 16-17 0.5915299440980917 1101 18-19 0.5085392887606233 1101 20-21 0.6963696541489313 1101 22-23 0.6586647681710005 1101 24-25 0.7363185810997379 1101 26-27 0.5704814498758495 1101 28-29 0.6983685399425141 1101 30-31 0.4689521806277597 1101 32-33 0.7092567323868266 1101 34-35 0.6223208033728653 1101 36-37 0.6817564230323327 1101 38-39 0.6201917187576882 1101 40-41 0.8453520250785331 1101 42-43 0.46266648908966346 1101 44-45 0.5269082761236135 1101 46-47 0.5200337930276859 1101 48-49 0.5623413362102738 1101 50-51 0.1425007864484087 1101 52-53 -0.013815950308895708 1101 54-55 0.03561080916506043 1101 56-57 0.1657661522477909 1101 58-59 0.17769433120102462 1101 60-61 0.18359868925294975 1101 62-63 -0.2997947930767708 1101 64-65 -0.21607830891001711 1101 66-67 -0.5229174211327603 1101 68-69 -6.955583275314417 1101 70-71 -7.869928166213416 1101 72-73 -5.22818958312828 1101 74-75 -3.2013187956461557 1101 76-77 -0.6369512985412484 1101 78-79 -0.3822425996711267 1101 80-81 -0.427235795816415 1101 82-83 -0.7447439980959523 1101 84-85 -0.6803236080032455 1101 86-87 -0.817934297935615 1101 88-89 -0.877855701208933 1101 90-91 -0.9250256942969983 1101 92-93 -0.8555475268374693 1101 94-95 -0.49656569491626357 1101 96-97 -0.8151647016664398 1101 98-99 -0.804547473797399 1101 100-101 -0.8404904642022473 1102 1 0.27069049682368984 1102 2 0.34078618573563446 1102 3 0.28741221632548175 1102 4 0.3098614317362376 1102 5 0.3518219552101556 1102 6 0.3232571851483783 1102 7 0.32655443839351506 1102 8 0.472977806892807 1102 9 0.6129732622756805 1102 10-11 0.5544628771242444 1102 12-13 0.5055809332537251 1102 14-15 0.633649656203815 1102 16-17 0.5430432069459741 1102 18-19 0.5083794370513459 1102 20-21 0.4506485130337765 1102 22-23 0.5362994957126404 1102 24-25 0.38505292693486837 1102 26-27 0.5073585933419551 1102 28-29 0.5025651317103055 1102 30-31 0.25819577525674475 1102 32-33 0.5251374345717963 1102 34-35 0.37972565123925506 1102 36-37 0.5228735119873846 1102 38-39 0.5681091254785642 1102 40-41 0.4060149414195777 1102 42-43 0.36899298378388323 1102 44-45 0.41880209906604193 1102 46-47 0.5177183652386859 1102 48-49 0.5132478891615548 1102 50-51 0.31514426546746677 1102 52-53 0.23853338541844948 1102 54-55 -0.02192934752191178 1102 56-57 0.0940044410917551 1102 58-59 0.23742841223130995 1102 60-61 0.2620075673200013 1102 62-63 -0.07990342765253189 1102 64-65 -0.12442071127130205 1102 66-67 -0.0844126966266856 1102 68-69 -6.760875820089041 1102 70-71 -7.72685886807545 1102 72-73 -1.7777569339817951 1102 74-75 -0.2909027613894466 1102 76-77 0.20655993305965836 1102 78-79 0.03158925570667037 1102 80-81 -0.08812533021198377 1102 82-83 -0.13200333246697582 1102 84-85 -0.5589465097477309 1102 86-87 -0.6122992022506395 1102 88-89 -0.6178044194888663 1102 90-91 -0.6747697700957751 1102 92-93 -0.6910032010887512 1102 94-95 -0.39765099114246993 1102 96-97 -0.431370435660277 1102 98-99 -0.5518905446294653 1102 100-101 -0.633078834748197 1103 1 0.2308501383852679 1103 2 0.18140753742281746 1103 3 0.29737475118496093 1103 4 0.27431610580203625 1103 5 0.35281374856032954 1103 6 0.2883006753845976 1103 7 0.3986775725700511 1103 8 0.3957802901712171 1103 9 0.45004606734114105 1103 10-11 0.4349022865709742 1103 12-13 0.43761296328843713 1103 14-15 0.4255268099799068 1103 16-17 0.47506643408112126 1103 18-19 0.5387408332459813 1103 20-21 0.42283604531107954 1103 22-23 0.48781645048843103 1103 24-25 0.5131619154386797 1103 26-27 0.7554677305169477 1103 28-29 0.5443685367501061 1103 30-31 0.3948973718288542 1103 32-33 0.67876368145361 1103 34-35 0.5008011229964211 1103 36-37 0.7106420982338975 1103 38-39 0.7856319034702182 1103 40-41 0.8682383738774675 1103 42-43 0.5247970680162837 1103 44-45 0.5812619578440632 1103 46-47 0.5385898444701027 1103 48-49 0.434399535342898 1103 50-51 0.14383181965420988 1103 52-53 0.02756778949735761 1103 54-55 0.06023183083031114 1103 56-57 0.3752146324237593 1103 58-59 0.46993822453001854 1103 60-61 0.24361928824383128 1103 62-63 0.030211456055294406 1103 64-65 -0.03236879059599218 1103 66-67 -0.33977894419708576 1103 68-69 -7.127335442558113 1103 70-71 -7.854562923199598 1103 72-73 -1.4157537305088361 1103 74-75 0.0860209655939066 1103 76-77 0.24614344245097897 1103 78-79 0.10148232199524543 1103 80-81 -0.35370274564937176 1103 82-83 -0.334734891761741 1103 84-85 -0.6308523101151842 1103 86-87 -0.5866164493609425 1103 88-89 -0.44574098853333055 1103 90-91 -0.7316012896405582 1103 92-93 -0.5087747685839084 1103 94-95 -0.38023866019726427 1103 96-97 -0.390283977884458 1103 98-99 -0.49449016215326225 1103 100-101 -0.33144259090240524 1104 1 0.28088177990585805 1104 2 0.27175185941246127 1104 3 0.2364132408758195 1104 4 0.2214615104042963 1104 5 0.355560048116196 1104 6 0.2786099553987924 1104 7 0.4711141771409544 1104 8 0.5026946252209115 1104 9 0.5543760567017557 1104 10-11 0.3931712129839724 1104 12-13 0.42112306263935295 1104 14-15 0.573863295938331 1104 16-17 0.5483655438535706 1104 18-19 0.49190792468132827 1104 20-21 0.4227547802035403 1104 22-23 0.6034892621804602 1104 24-25 0.42494460677766455 1104 26-27 0.6474551638190462 1104 28-29 0.46527136327734553 1104 30-31 0.41785966366626326 1104 32-33 0.6144305337044287 1104 34-35 0.4155802490449432 1104 36-37 0.7060390926928832 1104 38-39 0.6520392710455525 1104 40-41 0.6915181759825231 1104 42-43 0.415994327826013 1104 44-45 0.42903973177951826 1104 46-47 0.3468246510649067 1104 48-49 0.5185769307327988 1104 50-51 0.5099867466827561 1104 52-53 0.14924067496197324 1104 54-55 0.32205215771200457 1104 56-57 0.3346595975028137 1104 58-59 0.537850495100173 1104 60-61 0.5067833711411573 1104 62-63 0.22995561505364748 1104 64-65 0.2244865840246284 1104 66-67 0.13243011807273675 1104 68-69 -6.723061128441383 1104 70-71 -4.227242977284117 1104 72-73 0.1657018601271183 1104 74-75 0.8278105961661844 1104 76-77 1.1070628217008291 1104 78-79 0.5371494624201958 1104 80-81 0.27259072397880146 1104 82-83 0.26718048647834536 1104 84-85 0.19917653035276572 1104 86-87 0.387700894918396 1104 88-89 0.28128087005154967 1104 90-91 0.18674802583058892 1104 92-93 0.34978359993548835 1104 94-95 0.44537621696562724 1104 96-97 0.2142785680072734 1104 98-99 0.35110987980489483 1104 100-101 0.4361360316482603 1105 1 0.11974755999944975 1105 2 0.1875572126316314 1105 3 0.1764611943146015 1105 4 0.21765358833224013 1105 5 0.323448438138513 1105 6 0.11998044296844057 1105 7 0.318040644106631 1105 8 0.39967121290521845 1105 9 0.33978593638546073 1105 10-11 0.3836822469434651 1105 12-13 0.4302752652750854 1105 14-15 0.36758247340439 1105 16-17 0.43732784570972427 1105 18-19 0.41989881831040066 1105 20-21 0.4923637475041929 1105 22-23 0.676252646461954 1105 24-25 0.5753100837936742 1105 26-27 0.4821766379944492 1105 28-29 0.4420373452581785 1105 30-31 0.452983071465205 1105 32-33 0.6031413210267047 1105 34-35 0.6712043592171497 1105 36-37 0.7600624332719903 1105 38-39 0.7265320193879319 1105 40-41 0.8644488886911716 1105 42-43 0.6903376059753157 1105 44-45 0.78843754681224 1105 46-47 0.6149404156914855 1105 48-49 0.6599888091723187 1105 50-51 0.618668239669077 1105 52-53 0.4321654893293214 1105 54-55 0.45180693626040735 1105 56-57 0.629449984345186 1105 58-59 0.7573153819404581 1105 60-61 0.8555715732439886 1105 62-63 0.3878280977732942 1105 64-65 0.3441117964351328 1105 66-67 0.378880865214942 1105 68-69 -3.1012784098222497 1105 70-71 -3.2550207866917837 1105 72-73 0.7038568027966825 1105 74-75 1.1857684606022616 1105 76-77 1.2155771191055216 1105 78-79 0.7240070356968396 1105 80-81 0.6542584780809761 1105 82-83 0.4778906634703368 1105 84-85 0.6213691912827848 1105 86-87 0.4678274321783391 1105 88-89 0.47239642705955376 1105 90-91 0.6129786213694857 1105 92-93 0.3225957195465625 1105 94-95 0.5316440130469857 1105 96-97 0.4927633053849547 1105 98-99 0.6448871297431182 1105 100-101 0.731449070740144 1106 1 0.01815996951751586 1106 2 0.1924570018966243 1106 3 0.2112490685237738 1106 4 0.17471275324469104 1106 5 0.25279497646400984 1106 6 0.08459561978094854 1106 7 0.1463110717235665 1106 8 0.31617791098182124 1106 9 0.47251522374399 1106 10-11 0.31008696575932504 1106 12-13 0.11146053618384855 1106 14-15 0.17726525673958093 1106 16-17 0.3993775155276893 1106 18-19 0.3374935317348289 1106 20-21 0.26642971980987795 1106 22-23 0.17914495869774782 1106 24-25 0.34918041174741177 1106 26-27 0.6728818047223584 1106 28-29 0.6019835187370077 1106 30-31 0.4294218062615158 1106 32-33 0.5866900253097427 1106 34-35 0.5156829341097335 1106 36-37 0.6309287666850665 1106 38-39 0.700845470829023 1106 40-41 0.6031077166107579 1106 42-43 0.5537782298699909 1106 44-45 0.7493834619756612 1106 46-47 0.6262094731302241 1106 48-49 0.8570330055271285 1106 50-51 0.7059862885139694 1106 52-53 0.42115665509493994 1106 54-55 0.3007067301041104 1106 56-57 0.418312526588533 1106 58-59 0.6615457069485586 1106 60-61 0.8097486326225685 1106 62-63 0.4689654639557972 1106 64-65 0.46503498213186134 1106 66-67 0.7280196103514989 1106 68-69 -2.9524664126930986 1106 70-71 -3.1553591150133435 1106 72-73 0.8912688296111213 1106 74-75 1.4471062940418555 1106 76-77 1.2908201626181217 1106 78-79 0.9359787720991299 1106 80-81 0.747754061726905 1106 82-83 0.6992382571878899 1106 84-85 0.5489400496416117 1106 86-87 0.6451105950883687 1106 88-89 0.6228275695958558 1106 90-91 0.9584383751037073 1106 92-93 0.7607676210112508 1106 94-95 1.2195709536653467 1106 96-97 1.0650821764893834 1106 98-99 1.0240498902000326 1106 100-101 0.9899440992177944 1107 1 0.059452362597568253 1107 2 0.08397233348326338 1107 3 0.1398267138489686 1107 4 -0.13115847763397426 1107 5 0.17113975934281456 1107 6 0.08497136895336865 1107 7 0.09547989174511429 1107 8 0.1772707648448062 1107 9 0.3594129221302538 1107 10-11 0.015487609815011183 1107 12-13 0.18837637177057331 1107 14-15 0.13980668568687094 1107 16-17 0.10181658383780956 1107 18-19 0.17366389136760318 1107 20-21 0.12501357760142184 1107 22-23 0.15209191864084204 1107 24-25 0.07876325613663226 1107 26-27 0.4349368405715879 1107 28-29 0.30147471674261084 1107 30-31 0.28026429072784964 1107 32-33 0.1651699818524932 1107 34-35 0.16967206779580835 1107 36-37 0.4622857153937048 1107 38-39 0.6004592174864456 1107 40-41 0.423439288244694 1107 42-43 0.22688995522496214 1107 44-45 0.401656714456891 1107 46-47 0.6823587643988631 1107 48-49 0.5311988493671294 1107 50-51 0.4820957039518987 1107 52-53 0.3850551227866106 1107 54-55 0.39146246012893116 1107 56-57 0.763129819053642 1107 58-59 0.9070788836196968 1107 60-61 0.8597855972975488 1107 62-63 0.7509575147999854 1107 64-65 0.5156216650449785 1107 66-67 0.7908501540773578 1107 68-69 -2.2453256778213557 1107 70-71 -2.4969894422532555 1107 72-73 1.239654138578782 1107 74-75 1.4557837389875026 1107 76-77 1.142258483631565 1107 78-79 1.13607230923337 1107 80-81 1.0218697856693062 1107 82-83 0.7886224427095421 1107 84-85 0.6050491249445109 1107 86-87 0.7677106673170435 1107 88-89 0.7008318372901314 1107 90-91 0.8305999232383172 1107 92-93 0.8080674990628403 1107 94-95 1.089381268360281 1107 96-97 0.9204024386334524 1107 98-99 1.1041369847765772 1107 100-101 1.3025738132142521 1108 1 -0.021806642368744633 1108 2 -0.020977077722736936 1108 3 -0.06671803529983578 1108 4 0.07098400004981187 1108 5 -0.18687596866092093 1108 6 -0.2225408858499449 1108 7 0.012544655060388266 1108 8 0.10625528636415993 1108 9 0.12972470525443924 1108 10-11 0.10945247132968916 1108 12-13 -0.031248820402453248 1108 14-15 0.06891838349025647 1108 16-17 0.2015736006685458 1108 18-19 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62-63 0.9874221385900412 2311 64-65 1.0234813549703112 2311 66-67 1.3861521757327786 2311 68-69 3.5627170295114894 2311 70-71 4.927557215532406 2311 72-73 3.842963907807569 2311 74-75 2.755062586615697 2311 76-77 2.2955155678894883 2311 78-79 1.7336148221408827 2311 80-81 1.3544911665675414 2311 82-83 1.3229907075892164 2311 84-85 1.4220172428705276 2311 86-87 1.2764396899280328 2311 88-89 1.3573530590907943 2311 90-91 1.5397374230389858 2311 92-93 1.6282877332809598 2311 94-95 1.7196309055761105 2311 96-97 1.8335964971916638 2311 98-99 1.8078888416304686 2311 100-101 1.9815075528167903 2312 1 -0.10124757988471345 2312 2 -0.13764774045360895 2312 3 -0.006946086098327697 2312 4 0.10770373575929426 2312 5 -1.1682195895667604 2312 6 -0.23670267535300837 2312 7 0.04531025248509479 2312 8 0.21376284285605607 2312 9 -2.75745117135758 2312 10-11 -0.15383796327176924 2312 12-13 -1.128208241353093 2312 14-15 -3.8995045206395744 2312 16-17 -0.9107167042246047 2312 18-19 -0.04954425435745691 2312 20-21 0.2527041778887522 2312 22-23 0.41870516393237267 2312 24-25 0.5742818151024309 2312 26-27 0.35556429045895044 2312 28-29 0.4343178403347565 2312 30-31 0.18292530866475687 2312 32-33 0.3981215529476856 2312 34-35 0.44904347768935793 2312 36-37 0.7337147392901642 2312 38-39 0.7452063549031109 2312 40-41 0.3294718072926841 2312 42-43 0.18899984193785713 2312 44-45 0.6966070642507702 2312 46-47 0.6053844750720714 2312 48-49 0.5922356632403023 2312 50-51 0.7554845110547035 2312 52-53 0.7208234150678408 2312 54-55 0.6125676618083453 2312 56-57 0.8425415166878132 2312 58-59 0.7868360676333168 2312 60-61 0.7421505890246252 2312 62-63 0.8161015244690404 2312 64-65 1.1610732044674172 2312 66-67 1.2900692307026134 2312 68-69 3.7941240017770888 2312 70-71 4.678261342597541 2312 72-73 2.830164728680156 2312 74-75 2.142740392925944 2312 76-77 1.9539757549099548 2312 78-79 1.3785222852887706 2312 80-81 1.1593370043709292 2312 82-83 1.1901324728366625 2312 84-85 1.2521086266434374 2312 86-87 1.258151704054903 2312 88-89 1.180571791326635 2312 90-91 1.3052228274523117 2312 92-93 1.6678922600602526 2312 94-95 1.8521770601917495 2312 96-97 2.0100650858167306 2312 98-99 1.8693079796303884 2312 100-101 1.89786648211237 2313 1 -0.2656138825793164 2313 2 -0.20075640995032984 2313 3 -0.06288842088014235 2313 4 -0.020296226007509688 2313 5 0.13888796160897243 2313 6 0.064634650163228 2313 7 -2.6859856497526273 2313 8 -3.058229309780419 2313 9 -0.8420869225024816 2313 10-11 -0.02369992948556643 2313 12-13 0.044204356962978864 2313 14-15 -0.5574446594021794 2313 16-17 -0.2449315915143373 2313 18-19 0.17719831316893675 2313 20-21 -2.64778198109682 2313 22-23 -0.7219696997722593 2313 24-25 0.00501371795665051 2313 26-27 -1.5499674556391696 2313 28-29 -1.5805862361603076 2313 30-31 -0.8516476564032374 2313 32-33 -0.6590439454160517 2313 34-35 -1.3768365728262495 2313 36-37 -2.2323422472887913 2313 38-39 -1.6412948177807039 2313 40-41 -0.906925837064513 2313 42-43 -0.022897791068864137 2313 44-45 -0.3991028724374459 2313 46-47 -0.19772890188693282 2313 48-49 -2.116818077101332 2313 50-51 -0.5027129251705347 2313 52-53 -0.28431207979517126 2313 54-55 -0.28013236650811635 2313 56-57 -0.3943286660788168 2313 58-59 -0.2538494296790077 2313 60-61 0.08384036487056434 2313 62-63 0.455978568899603 2313 64-65 0.3779575813081415 2313 66-67 1.1397321214428935 2313 68-69 1.9537004018588107 2313 70-71 4.279007905492943 2313 72-73 3.493800120194063 2313 74-75 2.1482635962942886 2313 76-77 2.1656200929917695 2313 78-79 1.421541799751072 2313 80-81 1.3962854457110474 2313 82-83 1.263631312100724 2313 84-85 1.190213263732744 2313 86-87 1.132198114375953 2313 88-89 0.833920660878551 2313 90-91 1.5632840579160465 2313 92-93 1.407142757987895 2313 94-95 1.4101669618488017 2313 96-97 1.4343815778253948 2313 98-99 1.7611725528702564 2313 100-101 1.7403319736658212 2314 1 -0.2930037859090717 2314 2 -0.22922042713614843 2314 3 -0.3051010954236446 2314 4 -0.10031770828462783 2314 5 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2314 66-67 -1.622620187901898 2314 68-69 -1.9356658709661083 2314 70-71 1.0609627927109848 2314 72-73 0.6342405068750487 2314 74-75 0.29675983689579155 2314 76-77 0.48302073746008034 2314 78-79 -1.8115409070158428 2314 80-81 -1.3134352846863742 2314 82-83 -0.06021401550400185 2314 84-85 -0.14141938932847964 2314 86-87 -0.5748373313812962 2314 88-89 -0.5747796613556027 2314 90-91 -0.12925085078427756 2314 92-93 -0.4752417747725808 2314 94-95 -0.11131531527257366 2314 96-97 0.3390539730992934 2314 98-99 0.05064623708078386 2314 100-101 0.05209352038977144 2315 1 -0.42272319205653375 2315 2 -0.30255121006215546 2315 3 -0.44078551194439797 2315 4 -0.1014321381950225 2315 5 -0.17896851912319534 2315 6 -0.08566301179989466 2315 7 -1.0824737388599317 2315 8 -3.1678136424320087 2315 9 -0.8697455014351405 2315 10-11 -1.5384650222566378 2315 12-13 -0.748243592656678 2315 14-15 -0.49092817249018594 2315 16-17 -0.24697799911847795 2315 18-19 -0.06394274887953344 2315 20-21 -0.6268252840704989 2315 22-23 -0.16498737115227158 2315 24-25 -5.230169249246089 2315 26-27 -0.8562910434737248 2315 28-29 -0.7587368475223784 2315 30-31 -0.9848925102207602 2315 32-33 -0.17518699383946057 2315 34-35 -2.0455480776156207 2315 36-37 -3.5565254057720814 2315 38-39 -0.9996905625241652 2315 40-41 -0.04876154178619885 2315 42-43 -1.455670034789712 2315 44-45 -4.991776680244637 2315 46-47 -3.3530891714453688 2315 48-49 -2.3763856359201014 2315 50-51 -0.9854925258356033 2315 52-53 0.05539805660257002 2315 54-55 -0.9237945062017694 2315 56-57 -0.9642599522198019 2315 58-59 -0.29833878418072146 2315 60-61 -1.418820200437029 2315 62-63 -0.37640978947186454 2315 64-65 0.2693321527661361 2315 66-67 0.6590499855748426 2315 68-69 3.1482125628876076 2315 70-71 3.6080681294383226 2315 72-73 -2.0453566268594834 2315 74-75 -1.8231827789392945 2315 76-77 -0.21180585936465945 2315 78-79 -0.12813214048111377 2315 80-81 -0.5369357187460828 2315 82-83 -1.2199824188999706 2315 84-85 -0.6889078757107789 2315 86-87 -0.37199975799578056 2315 88-89 -0.3206528570650313 2315 90-91 -0.3520436939489393 2315 92-93 -1.535964079556063 2315 94-95 -0.3132348891583625 2315 96-97 -0.34883399535412707 2315 98-99 -2.6835036680146374 2315 100-101 -3.864332917524976 2316 1 -0.4860208288998855 2316 2 -0.3366404594556407 2316 3 -0.8883570887163579 2316 4 -0.24770540830390786 2316 5 -0.14342592430694623 2316 6 -0.4210339040487767 2316 7 -0.5339008632912012 2316 8 -0.9252281383109846 2316 9 -5.071892845436576 2316 10-11 -1.3157282369011796 2316 12-13 -0.5256152854218001 2316 14-15 -0.37469991099910516 2316 16-17 -0.23489788214508422 2316 18-19 -0.3477940489015907 2316 20-21 -3.6073944674622354 2316 22-23 -1.0946806427314968 2316 24-25 -0.4359788734118766 2316 26-27 -0.3804401682153937 2316 28-29 0.010573973333528386 2316 30-31 0.1111673161162301 2316 32-33 -1.224521176700243 2316 34-35 -0.4042620237523167 2316 36-37 -0.24914088840986892 2316 38-39 -5.192792236256615 2316 40-41 -4.763664540454727 2316 42-43 -3.5133848059482524 2316 44-45 -2.001159220821826 2316 46-47 -1.9712306667770534 2316 48-49 -2.351495461848657 2316 50-51 -0.9859352603337683 2316 52-53 -0.7167181652874604 2316 54-55 0.1814555734414256 2316 56-57 -2.8091306851822857 2316 58-59 -0.4509068893260775 2316 60-61 -0.23415466102794014 2316 62-63 0.23293517585270962 2316 64-65 0.3811137915372633 2316 66-67 0.3650007619939224 2316 68-69 2.816505094397723 2316 70-71 3.89539105606039 2316 72-73 2.7431708316647914 2316 74-75 1.227602788896288 2316 76-77 -1.2547805124062137 2316 78-79 0.5379552138126762 2316 80-81 0.5202311072069818 2316 82-83 0.6333538551053088 2316 84-85 0.9639279939521082 2316 86-87 0.9611751057939308 2316 88-89 0.7400128783888498 2316 90-91 0.7643182047449102 2316 92-93 0.7820086538801938 2316 94-95 1.1453834851401652 2316 96-97 1.2430624929934595 2316 98-99 1.1829551911680198 2316 100-101 1.3368930159341517 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 41.0 4 111.0 5 262.0 6 637.0 7 1446.0 8 2856.0 9 4798.0 10 7562.0 11 10923.0 12 14019.0 13 17403.0 14 21776.0 15 27473.0 16 32769.0 17 35906.0 18 31161.0 19 25263.0 20 24641.0 21 24855.0 22 26481.0 23 29376.0 24 34850.0 25 42353.0 26 52351.0 27 64675.0 28 78523.0 29 92920.0 30 108818.0 31 125545.0 32 144293.0 33 165234.0 34 190569.0 35 209404.0 36 206361.0 37 166042.0 38 67670.0 39 3655.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.90737702685332 21.081329318533818 16.303329222002773 20.707964432610098 2 15.78323886837011 22.307652976136147 39.2692468878171 22.63986126767664 3 19.03329253108663 27.282704147400267 30.996473042328272 22.687530279184834 4 12.770951355725188 18.737548169395588 36.789558204507 31.701942270372225 5 12.667412633920552 39.350855600869934 33.11229833084253 14.869433434366986 6 29.61827626142362 37.673862606974 17.38568736646167 15.322173765140715 7 26.674747447721774 35.46792404511732 20.25234205688207 17.60498645027883 8 26.65439419941979 34.8138967774979 19.188335390954368 19.343373632127935 9 26.916263105389692 17.16796494272424 20.49916102572727 35.416610926158796 10-11 24.710901524704475 28.209469710182393 26.06277525935362 21.01685350575951 12-13 26.195538338639828 26.00418913805427 27.361669577913762 20.43860294539214 14-15 22.96221462452557 26.547730756147065 27.36100069181933 23.129053927508036 16-17 20.56518742631768 30.090837532802446 27.738091683199773 21.6058833576801 18-19 20.34926859659508 27.297923442127708 32.172098741012434 20.180709220264774 20-21 21.86643586911237 26.12819584220404 32.21181042621422 19.793557862469374 22-23 21.204852491223242 26.481083865098793 32.076732848294434 20.237330795383528 24-25 20.76600014333835 26.69069973004611 32.46109558278984 20.082204543825704 26-27 20.65750467579744 26.78677097515311 32.35207482613726 20.203649522912183 28-29 20.260352439956065 26.879029187165465 32.54245442805072 20.31816394482776 30-31 20.438608875711797 26.856229483955413 32.38965201826159 20.315509622071197 32-33 20.428940820328176 26.94750064022444 32.05739946565148 20.5661590737959 34-35 20.767269805799568 26.947684064699555 32.05980602173996 20.22524010776092 36-37 20.840147315912493 26.67946739043116 32.04752286629101 20.432862427365336 38-39 20.646887095567042 27.033286713554254 32.03639642577974 20.283429765098962 40-41 20.65265127327435 26.995295343372515 32.03407606773998 20.317977315613152 42-43 20.540164347326517 27.04967357420171 31.601369154620894 20.808792923850874 44-45 20.681149857271958 27.327208220370185 31.259401641970175 20.732240280387686 46-47 20.933765831999708 27.599498520856475 30.55455809063059 20.912177556513228 48-49 21.023529077079644 27.70874367116523 30.19485603402504 21.072871217730086 50-51 21.077513922241042 28.002644000616773 29.921050524078694 20.99879155306349 52-53 21.222150780770086 28.152659494369804 29.511316565041867 21.113873159818247 54-55 20.75874531762252 28.082888328717775 29.75739470879693 21.400971644862775 56-57 21.135978054643555 28.382676932046813 29.266393305519316 21.214951707790313 58-59 21.246467249238016 27.88285365021443 29.48531513044775 21.38536397009981 60-61 21.308005183624946 27.945749695137103 29.727238496442947 21.019006624795004 62-63 21.245558368661733 27.880687880109754 29.780227340811983 21.09352641041653 64-65 21.03326904428634 27.955534245325186 29.687181585858607 21.324015124529865 66-67 20.93563093095579 28.258613239594467 29.47460422810664 21.33115160134311 68-69 21.277302197938365 27.688811906415765 29.82226849297666 21.21161740266921 70-71 20.82644930819162 27.797958601384583 30.14422233489425 21.23136975552955 72-73 21.142074497045094 27.92971517302339 29.3983576424908 21.52985268744071 74-75 20.951860361562957 28.259053593891426 29.138402506183837 21.65068353836178 76-77 20.372462495727543 28.347260505456777 29.62109889983638 21.6591780989793 78-79 20.882463688613736 28.291104642161542 29.037855778953066 21.788575890271662 80-81 20.631761915591802 28.51880006039259 29.019666770506248 21.829771253509364 82-83 20.792077788764125 28.498164000360248 28.826042408474144 21.88371580240148 84-85 20.671142158087633 28.342774992606408 28.74140778746974 22.24467506183622 86-87 20.67748815573465 28.26070857278275 28.855979657868435 22.205823613614168 88-89 20.72038692267853 28.132471233065203 28.73564042143727 22.411501422818997 90-91 20.481656576013638 28.348017994752368 28.87985270505391 22.290472724180084 92-93 20.594540138375486 28.280349758723244 28.87009756902828 22.255012533872993 94-95 20.27376903624291 28.01308265809088 28.97733222215369 22.735816083512514 96-97 20.37103640081643 27.913856427538548 28.853145225447236 22.861961946197788 98-99 20.313724834339382 27.84617902456448 28.60407859008987 23.23601755100627 100-101 20.224191601200996 27.743937621236224 28.965767034832172 23.066103742730604 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7747.0 1 5174.5 2 2715.0 3 2903.0 4 2722.5 5 2584.5 6 3637.0 7 6501.5 8 9449.0 9 10544.0 10 9337.0 11 7253.0 12 6249.5 13 5987.0 14 5797.0 15 5666.0 16 5635.5 17 5845.0 18 6073.5 19 6224.0 20 6922.5 21 7811.0 22 8623.0 23 9820.5 24 11046.5 25 12615.5 26 15338.5 27 18325.0 28 20916.5 29 23856.0 30 27498.0 31 31634.5 32 35994.5 33 40447.0 34 45446.5 35 50001.5 36 54903.0 37 59452.5 38 61123.0 39 61203.5 40 61126.5 41 63048.5 42 65900.5 43 67872.0 44 69554.5 45 70219.5 46 70282.0 47 71765.5 48 77074.0 49 77430.5 50 71139.0 51 66327.5 52 62371.5 53 59898.5 54 57525.0 55 53375.5 56 48776.0 57 44530.5 58 40507.0 59 36286.0 60 31487.5 61 25882.0 62 20647.0 63 15801.5 64 11658.0 65 8878.5 66 6621.0 67 4900.5 68 3540.5 69 2516.0 70 1937.5 71 1544.5 72 1322.5 73 1171.0 74 907.0 75 703.0 76 515.0 77 313.5 78 197.0 79 122.0 80 77.5 81 55.0 82 35.5 83 18.5 84 8.5 85 6.5 86 4.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02106991197458997 2 0.017629926346085487 3 4.7777578173673405E-4 4 0.09431293931483131 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011132175714465904 12-13 0.0 14-15 0.0 16-17 4.5388699264989736E-4 18-19 4.777757817367341E-5 20-21 0.0 22-23 1.9111031269469363E-4 24-25 0.003917761410241219 26-27 0.0035116519957649954 28-29 0.0010988842979944883 30-31 2.6277667995520373E-4 32-33 7.644412507787745E-4 34-35 0.0031294313703756084 36-37 0.08332409633488642 38-39 0.09567460029278099 40-41 0.07613357081974857 42-43 0.07971688918277407 44-45 0.07845078336117174 46-47 0.07735189906317726 48-49 0.0723113645658547 50-51 0.07188136636229164 52-53 0.05671198529215033 54-55 0.04837479790084433 56-57 0.026850998933604455 58-59 0.022455461741626503 60-61 0.01375994251401794 62-63 0.0024366564868573437 64-65 0.006712749733401114 66-67 0.15551601695530692 68-69 0.7131042430311625 70-71 0.8685963711973825 72-73 0.3551068497758276 74-75 0.05191033868569615 76-77 0.19445474316685077 78-79 0.014333273452102023 80-81 0.0028666546904204046 82-83 0.0014572161342970389 84-85 1.4333273452102024E-4 86-87 0.0017199928142522425 88-89 1.9111031269469363E-4 90-91 0.004228315668370097 92-93 0.004944979340975197 94-95 0.0745807995291042 96-97 0.036191515466557606 98-99 0.01311494520867335 100-101 0.01282827973963131 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2093032.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.64714345152559 #Duplication Level Percentage of deduplicated Percentage of total 1 72.12967483566979 34.367729640070536 2 14.247487654227847 13.577041761696679 3 5.525706808563989 7.8985243493615975 4 2.6579974912558795 5.06583951038656 5 1.4995023801943177 3.572350250751135 6 0.9082185949746434 2.5964413008059903 7 0.6154749828009617 2.0527937357439865 8 0.4145730971907239 1.5802579066391744 9 0.3062839320517845 1.3134199002631823 >10 1.504238679325105 13.131076654952725 >50 0.11616893209618075 3.8327542775069467 >100 0.0704010576310401 6.052584606702805 >500 0.0027459990113633116 0.8901413899521649 >1k 0.0011187403379628305 1.1241009212651947 >5k 1.0170366708753003E-4 0.47736854659582845 >10k+ 3.051110012625901E-4 2.46757524730547 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20472 0.978102580371442 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17479 0.8351042888976374 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12970 0.6196751889125441 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9851 0.4706569225888567 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4399 0.21017356638598933 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3900 0.18633255487732628 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3052 0.14581716858605123 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.777757817367341E-5 0.0 0.0 0.0 0.0 6 4.777757817367341E-5 0.0 0.0 0.0 0.0 7 9.555515634734681E-5 0.0 0.0 0.0 0.0 8 9.555515634734681E-5 0.0 0.0 0.0 0.0 9 9.555515634734681E-5 0.0 0.0 0.0 0.0 10-11 1.4333273452102024E-4 0.0 0.0 0.0 0.0 12-13 2.6277667995520373E-4 0.0 0.0 0.0 0.0 14-15 2.8666546904204047E-4 0.0 0.0 0.0 0.0 16-17 2.8666546904204047E-4 0.0 0.0 1.6722152360785695E-4 0.0 18-19 3.583318363025506E-4 0.0 0.0 2.3888789086836705E-4 0.0 20-21 3.8222062538938726E-4 0.0 0.0 4.777757817367341E-4 0.0 22-23 3.8222062538938726E-4 0.0 0.0 0.001051106719820815 0.0 24-25 3.8222062538938726E-4 0.0 0.0 0.0017199928142522427 0.0 26-27 4.299982035630607E-4 0.0 0.0 0.0024605452759441804 0.0 28-29 6.44997305344591E-4 0.0 0.0 0.00456275871558581 0.0 30-31 6.688860944314277E-4 0.0 0.0 0.010343845674600293 0.0 32-33 6.688860944314277E-4 0.0 0.0 0.01934991916033773 0.0 34-35 7.644412507787745E-4 0.0 0.0 0.03267986347079261 0.0 36-37 8.599964071261214E-4 0.0 0.0 0.05398866333625095 0.0 38-39 9.79440352560305E-4 0.0 0.0 0.08712241379969346 0.0 40-41 0.0010272179307339781 0.0 0.0 0.12326615168807739 0.0 42-43 0.0011466618761681617 0.0 0.0 0.15766600797312225 0.0 44-45 0.0011944394543418353 0.0 0.0 0.20231415477642004 0.0 46-47 0.001289994610689182 0.0 0.0 0.2544633813529846 0.0 48-49 0.001289994610689182 0.0 0.0 0.3124892500449109 0.0 50-51 0.001289994610689182 0.0 0.0 0.3782311976118855 0.0 52-53 0.001289994610689182 0.0 0.0 0.44320870392808137 0.0 54-55 0.001289994610689182 0.0 0.0 0.5158545115411518 0.0 56-57 0.0014333273452102023 0.0 0.0 0.6031441468644532 0.0 58-59 0.0014333273452102023 2.3888789086836704E-5 0.0 0.6917476655875304 0.0 60-61 0.001457216134297039 4.777757817367341E-5 0.0 0.7833372829464623 0.0 62-63 0.001528882501557549 9.555515634734681E-5 0.0 0.8734696841710973 0.0 64-65 0.001528882501557549 9.555515634734681E-5 0.0 0.9695742826674414 0.0 66-67 0.001528882501557549 9.555515634734681E-5 0.0 1.073633847929702 0.0 68-69 0.0015766600797312224 9.555515634734681E-5 0.0 1.1932450148874933 0.0 70-71 0.0016483264469917326 9.555515634734681E-5 0.0 1.347614369966632 0.0 72-73 0.0016722152360785694 9.555515634734681E-5 0.0 1.5169858845923043 0.0 74-75 0.0017199928142522427 9.555515634734681E-5 0.0 1.693189592896812 0.0 76-77 0.001767770392425916 9.555515634734681E-5 0.0 1.8899854373941727 0.0 78-79 0.001767770392425916 9.555515634734681E-5 0.0 2.1223039112636597 0.0 80-81 0.0020544358614679563 9.555515634734681E-5 0.0 2.3858211436805554 0.0 82-83 0.0022933237523363238 9.555515634734681E-5 0.0 2.6632894289241635 0.0 84-85 0.0023888789086836705 9.555515634734681E-5 0.0 2.9354066254123206 0.0 86-87 0.0024844340650310172 9.555515634734681E-5 0.0 3.2179154451532517 0.0 88-89 0.0024844340650310172 9.555515634734681E-5 0.0 3.532626352583238 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11585 0.0 40.270473 1 GTATCAA 18120 0.0 34.180748 1 TATCAAC 23340 0.0 26.426954 2 ATCAACG 23525 0.0 26.215372 3 TCAACGC 23860 0.0 25.85163 4 CAACGCA 24330 0.0 25.371824 5 AACGCAG 24845 0.0 24.922235 6 GTACATG 16105 0.0 23.781652 1 TACATGG 16135 0.0 23.171913 2 ATAACGC 185 2.5750523E-7 23.078793 3 GAGTACT 14950 0.0 22.719332 12-13 GTGTACG 215 4.465437E-8 22.074495 1 ACGCAGA 28140 0.0 22.004013 7 ACATGGG 16295 0.0 21.951252 3 CGCAGAG 28230 0.0 21.900272 8 GTACTTT 16040 0.0 21.11633 14-15 AGTACTT 15360 0.0 21.063572 12-13 CATGGGG 4905 0.0 20.797659 4 TAACGCA 160 5.034343E-5 20.758362 4 GCAGAGT 28715 0.0 20.737846 9 >>END_MODULE