FastQCFastQC Report
Fri 27 May 2016
SRR939531_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939531_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834561
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100171.200271759643693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73460.8802232551005857No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55640.6666978207704409No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55310.6627436460606235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42340.5073325976171904No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30480.3652219550158706No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17000.2036999092936286No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14590.17482245156435539No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13200.15816698839269988No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13170.15780751796453465No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11390.13647893922673118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG10000.056.171162
GTACCTG11300.052.338661
ACCTGGG11650.047.7284283
ATAACGC1106.4695996E-834.8612063
GTACATG75250.027.7746491
TACATGG74750.027.705772
CCTGGGG7700.027.3708784
ATCACGC908.482505E-426.6300873
CATGGGG17650.026.5954974
CATGGGT6800.026.062714
TATAGGG2257.094059E-1126.0503942
ACATGGG76250.025.9630263
TATCACG950.001042035125.7076262
GGTATCA80300.025.4037551
GTATCAA108050.025.095251
GTATAGG2201.1623342E-925.0604611
TCAACGG1551.2091168E-624.9521485
ATGGGAT18250.024.6359755
TAACGCA2351.5825208E-1024.459084
CCTGGGA11350.024.0550214
Produced by FastQC (version 0.11.5)