FastQCFastQC Report
Fri 27 May 2016
SRR939531_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939531_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834561
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137271.6448168558080238No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102221.2248355722349833No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA97791.1717537723425848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90161.0803284601125622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69510.832892982058831No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45170.5412426413407768No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23200.27799046444777553No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22490.26948299764786515No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22340.26768564550703905No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.256542062233917No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17480.20945143614427225No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12440.14906040421251412No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11350.13599964532251088No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11020.1320454706126934No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8360.10017242598204326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG11200.056.2768632
GTACCTG12850.049.4253731
ACCTGGG14650.042.0383643
TATAACG1255.7771103E-934.1215482
TATCACG600.003994994331.5940272
CCTGGGA14450.029.1784524
ATAACGC1303.1065792E-729.1532343
GTACATG81050.028.5959221
TACATGG79050.028.2967382
ACGCGAT350.00849462127.07572678-79
GTACACG1606.370465E-826.6606541
ACATGGG80600.026.3319533
TCACGCA909.0662244E-426.3283542
TAACGCA1806.884875E-926.318894
GGTATCA85800.026.2394431
GGGACCG1109.6461845E-525.8403667
ATGGGCG1301.0225309E-525.5090795
CATGGGA61400.025.0758174
ATGGGAG17800.025.0177335
TGGGATA7600.023.6870026
Produced by FastQC (version 0.11.5)