##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939531_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 834561 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.99135952914167 31.0 31.0 31.0 30.0 34.0 2 31.171457808356728 31.0 31.0 34.0 28.0 34.0 3 31.15505637095431 31.0 31.0 34.0 28.0 34.0 4 35.1003893064737 35.0 35.0 37.0 32.0 37.0 5 34.77077169913284 35.0 35.0 37.0 32.0 37.0 6 34.760174510910524 35.0 35.0 37.0 32.0 37.0 7 34.6570400486004 35.0 35.0 37.0 30.0 37.0 8 34.69547462677983 35.0 35.0 37.0 31.0 37.0 9 36.030691585156745 37.0 35.0 39.0 30.0 39.0 10-11 35.87297693038616 37.0 35.0 39.0 30.0 39.0 12-13 35.830470151373 37.0 35.0 39.0 30.0 39.0 14-15 36.61038378261146 38.0 35.0 40.0 30.0 41.0 16-17 36.64102204632135 38.0 35.5 40.0 30.5 41.0 18-19 36.67652873786338 38.5 35.0 40.0 31.0 41.0 20-21 36.564402721910085 38.0 35.0 40.0 30.0 41.0 22-23 36.330394063465704 38.0 35.0 40.0 30.0 41.0 24-25 36.06898417251705 38.0 34.0 40.0 29.5 41.0 26-27 35.61825738322304 38.0 34.0 40.0 27.5 41.0 28-29 35.291437054930675 38.0 34.0 40.0 27.0 41.0 30-31 34.975656063487264 37.0 33.0 40.0 27.0 41.0 32-33 34.58139428993207 37.0 33.0 40.0 25.5 41.0 34-35 34.291381337014315 37.0 33.0 40.0 24.5 41.0 36-37 34.10427817738908 37.0 33.0 40.0 24.0 41.0 38-39 34.02664934019203 37.0 33.0 40.0 24.0 41.0 40-41 33.78207644498126 37.0 33.0 40.0 23.5 41.0 42-43 33.4314304167101 36.5 33.0 40.0 21.5 41.0 44-45 33.17344328335496 36.0 32.0 40.0 20.5 41.0 46-47 32.727094843876 36.0 31.5 40.0 19.0 41.0 48-49 32.335571635866046 36.0 31.0 39.5 15.0 41.0 50-51 32.05855054333955 36.0 31.0 39.0 13.0 41.0 52-53 31.696520685725787 35.0 30.0 39.0 9.0 40.0 54-55 31.508959201304638 35.0 30.0 39.0 8.5 40.0 56-57 31.154075615802796 35.0 29.5 39.0 8.0 40.0 58-59 30.768873096154742 35.0 28.5 38.5 6.5 40.0 60-61 30.519924846715817 35.0 28.0 38.0 3.5 40.0 62-63 30.158434194744302 34.5 28.0 38.0 2.0 40.0 64-65 29.7517221629096 34.0 28.0 38.0 2.0 40.0 66-67 28.21034472015826 33.5 24.0 36.5 2.0 39.5 68-69 24.788850665200027 29.5 13.0 35.0 2.0 38.0 70-71 24.231418674009447 29.5 10.5 35.0 2.0 38.0 72-73 25.962329895597804 31.0 20.0 35.0 2.0 38.0 74-75 26.523488396893697 31.5 22.0 35.0 2.0 37.5 76-77 26.50014858111031 31.5 23.5 34.5 2.0 36.5 78-79 27.294282263369603 32.5 26.0 35.0 2.0 37.0 80-81 27.167886469652906 33.0 25.5 35.0 2.0 36.5 82-83 26.776118222634416 33.0 25.0 35.0 2.0 36.0 84-85 26.44924637024735 32.0 24.5 35.0 2.0 36.0 86-87 25.992455913947573 32.0 23.5 35.0 2.0 35.0 88-89 25.497330932070874 32.0 20.5 34.0 2.0 35.0 90-91 25.048301442315182 31.0 19.5 34.0 2.0 35.0 92-93 24.53495730090431 31.0 17.0 34.0 2.0 35.0 94-95 23.2555097829877 31.0 4.5 34.0 2.0 35.0 96-97 22.47521631133015 30.0 2.0 34.0 2.0 35.0 98-99 22.18303155790889 30.0 2.0 34.0 2.0 35.0 100-101 21.088013937866734 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3545733435507792 1101 2 0.23973026274309106 1101 3 0.1270784794578823 1101 4 0.27665961593115895 1101 5 0.09912528460286296 1101 6 0.5184618184474203 1101 7 0.2421838018890341 1101 8 0.21250095025102667 1101 9 0.45519055811203657 1101 10-11 0.6985774145063814 1101 12-13 0.5888714577859844 1101 14-15 0.53623625158788 1101 16-17 0.5996267131075044 1101 18-19 0.6645930050298574 1101 20-21 0.7439384269147951 1101 22-23 0.7826865518806585 1101 24-25 0.5654673100390468 1101 26-27 0.8647648893276312 1101 28-29 0.5957187461380116 1101 30-31 0.7908350742147476 1101 32-33 0.5418168040721483 1101 34-35 0.9754637319748269 1101 36-37 0.7096496253333768 1101 38-39 0.7739485282652581 1101 40-41 0.6523156864409927 1101 42-43 0.8243474643646778 1101 44-45 0.7906010313758642 1101 46-47 0.8124449125065567 1101 48-49 0.8177844600993396 1101 50-51 0.9571647641131129 1101 52-53 0.7953778909183029 1101 54-55 0.6489802357164933 1101 56-57 0.5275166986888458 1101 58-59 0.43615580324805237 1101 60-61 0.30036238171217633 1101 62-63 0.46814283893060704 1101 64-65 -0.07218628568872631 1101 66-67 -2.9122562324842605 1101 68-69 -8.333102709884884 1101 70-71 -10.837423507222994 1101 72-73 -10.81218679618908 1101 74-75 -9.196756709385262 1101 76-77 -4.460021629972051 1101 78-79 -1.7537821043807824 1101 80-81 -1.3017500987455968 1101 82-83 -0.7796080600580595 1101 84-85 -0.8978838338282564 1101 86-87 -0.8922785056727669 1101 88-89 -0.6559111709931216 1101 90-91 -0.5832969594911894 1101 92-93 -1.449713071740458 1101 94-95 -4.864091004415865 1101 96-97 -1.7704434642625344 1101 98-99 -1.2687905317721295 1101 100-101 -1.1962219724168648 1102 1 0.32468262475748944 1102 2 0.3426495588284659 1102 3 0.5128000945622873 1102 4 0.31139872628978793 1102 5 0.45896059320683946 1102 6 0.5994633063136163 1102 7 0.5355294485977709 1102 8 0.5773436981859703 1102 9 0.7447626683167456 1102 10-11 0.6748472313449199 1102 12-13 0.7760912002312139 1102 14-15 0.7757121635472402 1102 16-17 0.9514006087516478 1102 18-19 1.0823640275530835 1102 20-21 1.05909285716465 1102 22-23 1.0734823037661414 1102 24-25 1.0115218993366781 1102 26-27 1.0013471580670483 1102 28-29 1.0117825704671901 1102 30-31 0.9256024027253389 1102 32-33 0.92817717552564 1102 34-35 1.0247031245149714 1102 36-37 1.0699902953862122 1102 38-39 0.8993589741120545 1102 40-41 1.1927304932749152 1102 42-43 1.248196933350357 1102 44-45 0.9505902571723865 1102 46-47 0.8860173305456485 1102 48-49 0.8995965785129698 1102 50-51 0.7816850555296604 1102 52-53 0.9231022252264438 1102 54-55 0.7274895477066465 1102 56-57 0.5776808770275679 1102 58-59 0.6817986640556022 1102 60-61 0.45475415227294747 1102 62-63 0.467802938463727 1102 64-65 0.13131661308506892 1102 66-67 -2.3849564530989014 1102 68-69 -7.4784133660851815 1102 70-71 -10.975511599162862 1102 72-73 -9.29994909673805 1102 74-75 -7.490746243016169 1102 76-77 -3.9022321373857274 1102 78-79 -1.0589139601090665 1102 80-81 -0.32233652324921636 1102 82-83 -0.1629837206193443 1102 84-85 -0.31204934611598034 1102 86-87 -0.3179531760334484 1102 88-89 -0.12384252392524076 1102 90-91 -0.26854271394196516 1102 92-93 -0.8827180483963524 1102 94-95 -3.98469410328201 1102 96-97 -1.131499849260738 1102 98-99 -0.24016706453083358 1102 100-101 -0.04987750573993921 1103 1 0.2813128281926218 1103 2 0.19970049185821281 1103 3 0.27192992676542715 1103 4 0.22531992611159524 1103 5 0.4561194310032022 1103 6 0.4450604948499759 1103 7 0.4897045711043333 1103 8 0.39411843461125073 1103 9 0.6398203330200047 1103 10-11 0.5573065195694369 1103 12-13 0.5628345056809039 1103 14-15 0.4501973885138355 1103 16-17 0.6987079393461144 1103 18-19 0.6977206577565127 1103 20-21 0.6614650296142841 1103 22-23 0.6929967323163595 1103 24-25 0.7167129600702324 1103 26-27 0.49478872615100045 1103 28-29 0.6875609202172228 1103 30-31 0.6252571569697594 1103 32-33 0.48624280934239295 1103 34-35 0.8128880475462026 1103 36-37 0.47133168032904393 1103 38-39 0.3614005025979239 1103 40-41 0.6893683688379539 1103 42-43 0.6574368977120884 1103 44-45 0.5518103729967763 1103 46-47 0.5289602103159794 1103 48-49 0.2648430765578169 1103 50-51 0.37113092990474783 1103 52-53 0.4106696657055835 1103 54-55 0.4181703871859348 1103 56-57 0.42042700384041964 1103 58-59 0.4837414448307342 1103 60-61 0.31264035729200756 1103 62-63 0.418057851100464 1103 64-65 0.13193285819717815 1103 66-67 -2.725700402017381 1103 68-69 -7.6585784405790704 1103 70-71 -11.073873228623833 1103 72-73 -8.883337702390676 1103 74-75 -6.830159943951674 1103 76-77 -3.412991678048005 1103 78-79 -0.7353865136902193 1103 80-81 -0.2281425916548976 1103 82-83 0.00858114598462123 1103 84-85 -0.20282670591403118 1103 86-87 0.21115017495403166 1103 88-89 0.22328290299155285 1103 90-91 0.13415119290355548 1103 92-93 0.11015305985489832 1103 94-95 0.8539882706228568 1103 96-97 1.1027562018207853 1103 98-99 0.5458882242876548 1103 100-101 0.28984555519160793 1104 1 0.38894087809975275 1104 2 0.2611702820965647 1104 3 0.3688608133657709 1104 4 0.07037880913325267 1104 5 0.2876780076970036 1104 6 0.38575444900777 1104 7 0.20957017583442195 1104 8 0.21102675992171527 1104 9 0.5164941959601705 1104 10-11 0.4769914881100519 1104 12-13 0.5050467276762092 1104 14-15 0.5970220328331521 1104 16-17 0.7721561213145876 1104 18-19 0.5212223255415154 1104 20-21 0.5867027983805215 1104 22-23 0.47263030584963417 1104 24-25 0.6867742742653675 1104 26-27 0.8999073187932964 1104 28-29 0.7690003694105627 1104 30-31 0.8835729858540518 1104 32-33 0.8046535998627675 1104 34-35 0.5651587635754112 1104 36-37 0.7670523287716335 1104 38-39 0.6804810814117062 1104 40-41 0.6438069622582319 1104 42-43 0.6496218905644326 1104 44-45 0.3319690800014712 1104 46-47 0.6590535187540993 1104 48-49 0.6003754063027387 1104 50-51 0.42986200321922396 1104 52-53 0.721997232295422 1104 54-55 0.28042131346303023 1104 56-57 0.3178339142079132 1104 58-59 0.16681367013244497 1104 60-61 0.3580145635138656 1104 62-63 0.2139584063804847 1104 64-65 -0.036272741281575094 1104 66-67 -2.8090629094324626 1104 68-69 -6.418469669877624 1104 70-71 -8.063137166541388 1104 72-73 -7.71964922323556 1104 74-75 -6.6045555542248415 1104 76-77 -3.8747189295577655 1104 78-79 -1.0558966225095432 1104 80-81 -0.1134544139652931 1104 82-83 -0.14590655713992362 1104 84-85 -0.21554538996845096 1104 86-87 -0.024611657567291445 1104 88-89 -0.18466922234030392 1104 90-91 0.09369281945585684 1104 92-93 -0.10748923672881361 1104 94-95 0.9531509744767526 1104 96-97 0.907638727518286 1104 98-99 0.5657448336758755 1104 100-101 0.09922088947796226 1105 1 0.410506885451575 1105 2 0.3705152256363071 1105 3 0.4176706693150223 1105 4 0.29834658248949353 1105 5 0.3676229913732101 1105 6 0.5257768666913094 1105 7 0.4083239875748248 1105 8 0.3457335434735924 1105 9 0.581948447733275 1105 10-11 0.6861355045953772 1105 12-13 0.6581037067709943 1105 14-15 0.9197865052099701 1105 16-17 0.923909889002779 1105 18-19 0.6392700291973625 1105 20-21 0.8663389270717232 1105 22-23 1.2495865913667359 1105 24-25 1.0339265431992573 1105 26-27 1.2044476339134889 1105 28-29 1.1901689278751917 1105 30-31 1.160847426268532 1105 32-33 1.3608997147019934 1105 34-35 1.6018811807694817 1105 36-37 1.576951106567357 1105 38-39 1.393286326562297 1105 40-41 1.467266480718557 1105 42-43 1.5889876854866856 1105 44-45 1.6116455898911894 1105 46-47 1.815649359245917 1105 48-49 1.2759039105341898 1105 50-51 1.1861321020430289 1105 52-53 1.4119871002856144 1105 54-55 1.2447757282293281 1105 56-57 1.4128349665664182 1105 58-59 1.2787417867043978 1105 60-61 1.5530251605192937 1105 62-63 1.6578114090285112 1105 64-65 1.3085594442467823 1105 66-67 1.9721052574569136 1105 68-69 -4.819204118221506 1105 70-71 -4.748284961727951 1105 72-73 -1.0924227507902167 1105 74-75 1.29643953640036 1105 76-77 1.2544421693981995 1105 78-79 1.2140076373397974 1105 80-81 1.2981572608510312 1105 82-83 1.291399796658112 1105 84-85 1.0687149977889483 1105 86-87 1.239641681835458 1105 88-89 1.3631788268807057 1105 90-91 1.1491557837784576 1105 92-93 1.0347076473110697 1105 94-95 1.7425960633888096 1105 96-97 1.8207946772596024 1105 98-99 1.760378539495175 1105 100-101 1.3933734471986021 1106 1 0.11740927369822174 1106 2 -0.022886568994309187 1106 3 0.11523720629693912 1106 4 -0.0056561546727991185 1106 5 0.22300676232205063 1106 6 0.1885618567896472 1106 7 0.16157799478526869 1106 8 0.15689352480672625 1106 9 0.40677852363391054 1106 10-11 0.3126984315213619 1106 12-13 0.21343512828189404 1106 14-15 0.08476219234167814 1106 16-17 0.09407518265531678 1106 18-19 0.11575086543430047 1106 20-21 0.3858986356558205 1106 22-23 0.2880718912093272 1106 24-25 0.4356988074272934 1106 26-27 0.45207498329097007 1106 28-29 0.6106485599541571 1106 30-31 0.37045493847332267 1106 32-33 0.588303013321088 1106 34-35 0.8562275140117421 1106 36-37 0.4515355148978131 1106 38-39 0.365608223618878 1106 40-41 0.5691435647218697 1106 42-43 0.35466894148631667 1106 44-45 0.3980949439257273 1106 46-47 0.21473947040082209 1106 48-49 0.25724732042908016 1106 50-51 0.268408371668027 1106 52-53 0.491059535949006 1106 54-55 0.6993622885690058 1106 56-57 0.43926236527086004 1106 58-59 0.49504410289500456 1106 60-61 0.4192481667069252 1106 62-63 0.2873200497082564 1106 64-65 0.46429297714471573 1106 66-67 0.9538186194998239 1106 68-69 -5.43439464357774 1106 70-71 -5.881693212386246 1106 72-73 -2.0500311915023666 1106 74-75 0.609546602486386 1106 76-77 0.586149002873789 1106 78-79 0.3861726069819582 1106 80-81 0.30611727700319236 1106 82-83 0.43725766249363573 1106 84-85 0.5669160147946606 1106 86-87 0.6841065448443544 1106 88-89 0.7186163980602274 1106 90-91 0.8996351498787512 1106 92-93 0.7896270436845683 1106 94-95 1.5976781903476827 1106 96-97 1.7565118645593643 1106 98-99 1.3433741605190939 1106 100-101 1.270681718893396 1107 1 -0.037860080505279115 1107 2 -0.14062601191118418 1107 3 -0.2189911214122553 1107 4 -0.03915320454310489 1107 5 0.04822908443144769 1107 6 0.011028569822045142 1107 7 0.014076297966909124 1107 8 -0.10882164672455019 1107 9 0.1767031214550414 1107 10-11 0.1011024885631926 1107 12-13 0.22972099339590812 1107 14-15 -0.2079087493895173 1107 16-17 0.010106315531452026 1107 18-19 -0.01565888836842788 1107 20-21 0.07595665666884344 1107 22-23 -0.017510431714065078 1107 24-25 0.13760941891634815 1107 26-27 0.029304458971104452 1107 28-29 -0.17267641398007783 1107 30-31 0.006649899611097965 1107 32-33 0.01755975301178836 1107 34-35 -0.09445103377508701 1107 36-37 0.12403819928241688 1107 38-39 -0.03144308314074351 1107 40-41 -0.07636384681314468 1107 42-43 -0.07130010040684454 1107 44-45 -0.016230991239652326 1107 46-47 -0.1791043409105768 1107 48-49 -0.1583055909760489 1107 50-51 -0.14705296541287893 1107 52-53 0.04174083695081521 1107 54-55 0.02788607455083536 1107 56-57 -0.10819647715785763 1107 58-59 0.3206841954509052 1107 60-61 0.16070452166576032 1107 62-63 0.29277919618767356 1107 64-65 -0.06116109384274182 1107 66-67 0.9604023944448485 1107 68-69 -5.400958044731933 1107 70-71 -5.201853808612906 1107 72-73 -1.8251078924804744 1107 74-75 0.05118323317372031 1107 76-77 0.20856412305162308 1107 78-79 0.3264392139557337 1107 80-81 0.2577396987071445 1107 82-83 0.3073723518531821 1107 84-85 0.2601641740310363 1107 86-87 0.47723176704676007 1107 88-89 0.20067800543501235 1107 90-91 0.2696977675637271 1107 92-93 0.375823620839828 1107 94-95 1.078453567508209 1107 96-97 0.9871301289087029 1107 98-99 0.6257618443111603 1107 100-101 0.6293723616733296 1108 1 -0.00959200982538988 1108 2 -0.02443307018615215 1108 3 0.053609978225409805 1108 4 -0.09409744553083499 1108 5 -0.13150873164152443 1108 6 0.08061076579457449 1108 7 0.014479433682787146 1108 8 -0.05362726731070211 1108 9 0.004348031745202263 1108 10-11 0.046210460936244147 1108 12-13 0.10629528693699086 1108 14-15 -0.13880011645673562 1108 16-17 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0.6431408404627241 2316 86-87 -0.05938189472224309 2316 88-89 0.6540360452944256 2316 90-91 0.7902347316822009 2316 92-93 1.1805537792502854 2316 94-95 1.6851182871932018 2316 96-97 1.723082152594742 2316 98-99 0.3733212356444042 2316 100-101 0.007498022556116979 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 49.0 5 169.0 6 483.0 7 953.0 8 1618.0 9 2274.0 10 3710.0 11 5326.0 12 6952.0 13 8177.0 14 8561.0 15 10444.0 16 12256.0 17 13828.0 18 11911.0 19 9342.0 20 8716.0 21 8858.0 22 9329.0 23 10800.0 24 12955.0 25 16018.0 26 19661.0 27 24392.0 28 29488.0 29 35282.0 30 41153.0 31 47618.0 32 55623.0 33 65137.0 34 77096.0 35 87525.0 36 83723.0 37 70662.0 38 32148.0 39 2309.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.80619010407928 20.02315536144896 14.303349617193456 18.86730491727831 2 15.63220209182004 23.54226365753308 40.548518503870476 20.2770157467764 3 18.96697784823398 32.070274072236785 30.015780751796456 18.946967327732782 4 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18.94980318029932 29.469822178617775 32.4976791826885 19.0826954583944 50-51 19.18005702960322 29.861803658649936 32.312337524099654 18.645801787647184 52-53 19.010580919331282 29.95758758876389 32.193921966919035 18.83790952498579 54-55 18.59622345610478 29.98214720344155 32.19774040427173 19.22388893618195 56-57 19.065664662698687 30.432304499248737 31.46920959435381 19.03282124369877 58-59 19.060892991751267 29.92386428596253 31.724911945984747 19.290330776301456 60-61 19.044263694782725 29.893197920879583 32.088617265087855 18.97392111924984 62-63 19.374546160427347 29.70393483402952 32.0514986591512 18.87002034639193 64-65 19.12231246011941 29.915011278905002 31.983619375151655 18.97905688582393 66-67 18.671269860749167 30.00287549238295 32.09777395982974 19.228080687038148 68-69 19.136813767790812 30.02292900703571 31.78212793504273 19.058129290130744 70-71 18.863179500073134 30.07823218471112 31.72646295515067 19.33212536006508 72-73 19.03152747239544 30.110598469283545 31.10171935262949 19.756154705691525 74-75 18.55985494872867 30.533424610357702 31.33793838320871 19.56878205770492 76-77 18.79693301087923 30.239299932648954 31.45006075974852 19.51370629672329 78-79 18.650040174165945 30.277959691620588 31.479050415732214 19.592949718481254 80-81 18.48256871829498 30.889576819082414 31.070692715881705 19.557161746740903 82-83 18.560596529193194 30.860236699294596 30.73004849256076 19.84911827895145 84-85 18.56694717342411 30.574277973689163 30.671754371459965 20.187020481426764 86-87 18.68756796266285 30.54379117077014 30.865218993298843 19.90342187326817 88-89 18.86598246969942 30.161223646134765 30.815883914181807 20.156909969984003 90-91 18.448439488385134 30.460891946882928 31.13863545403782 19.95203311069411 92-93 18.374547506839072 30.862519966017327 30.716451998279297 20.0464805288643 94-95 18.043211722308484 30.754007567815528 30.821976206173137 20.380804503702848 96-97 17.932842511179203 30.480211892979874 30.729646102455373 20.85729949338555 98-99 18.246106850469143 30.464805113329614 30.343706876354563 20.945381159846676 100-101 18.20020707109441 30.35952239435539 30.779716140041412 20.660554394508782 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8234.0 1 5503.5 2 6442.5 3 8986.5 4 6551.0 5 5140.5 6 5686.0 7 7458.5 8 8749.5 9 8552.0 10 7057.0 11 5305.5 12 4468.5 13 4187.0 14 4024.5 15 3715.0 16 3381.5 17 3308.0 18 3383.5 19 3516.0 20 3686.5 21 3885.5 22 4281.5 23 4823.0 24 5354.5 25 6168.0 26 7352.0 27 8571.0 28 9653.5 29 10783.5 30 12115.5 31 13597.0 32 15319.5 33 16774.0 34 18100.5 35 19532.0 36 21136.5 37 22568.5 38 22979.5 39 22831.0 40 22827.5 41 23100.5 42 23494.5 43 23909.5 44 24242.0 45 24639.0 46 25091.0 47 26762.0 48 31179.0 49 30869.5 50 26195.0 51 23398.5 52 20983.5 53 19566.0 54 18550.5 55 17035.5 56 15283.0 57 13618.5 58 12189.5 59 10882.0 60 9485.0 61 7961.0 62 6364.5 63 4681.0 64 3262.0 65 2364.5 66 1795.0 67 1418.5 68 1071.5 69 776.5 70 588.0 71 453.0 72 387.5 73 331.0 74 251.5 75 184.0 76 123.0 77 77.5 78 47.0 79 27.5 80 15.5 81 9.5 82 7.5 83 5.0 84 4.5 85 3.0 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023605224782849907 2 0.02013034397725271 3 0.0 4 0.0 5 1.1982347605507566E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.788525943579918E-4 18-19 0.001018499546468143 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.991173802753783E-4 28-29 0.019770873549087485 30-31 0.005272232946423329 32-33 0.010125083726653893 34-35 0.010724201106929272 36-37 0.0948402812975924 38-39 0.0972367508186939 40-41 0.1086199810439261 42-43 0.0972367508186939 44-45 0.0923239883004358 46-47 0.09340239958493148 48-49 0.09621825127222576 50-51 0.09388169348915178 52-53 0.07273284996543093 54-55 0.06206856059652919 56-57 0.035887131078495164 58-59 0.015577051887159837 60-61 0.00880702549004806 62-63 0.001797352140826135 64-65 0.004613203828120413 66-67 0.40727999511120216 68-69 0.7873001494198747 70-71 0.8723149056809508 72-73 0.2721191141210768 74-75 0.14384808300411833 76-77 0.015217581458994609 78-79 0.00832773158582776 80-81 0.0014977934506884456 82-83 0.0 84-85 0.0 86-87 4.1938216619276484E-4 88-89 7.18940856330454E-4 90-91 0.003235233853487043 92-93 0.002156822568991362 94-95 0.21855802032445798 96-97 0.2248487528173495 98-99 0.013719788008306163 100-101 0.007788525943579918 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 834561.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.66326791236167 #Duplication Level Percentage of deduplicated Percentage of total 1 85.3085123161796 54.31018674789946 2 9.446683253560781 12.028134537093207 3 2.3404545249992648 4.47002950385182 4 0.8745300302144223 2.2270175844378604 5 0.46684833288752786 1.4860545245529044 6 0.2997738348650985 1.1450749177279698 7 0.20983612935560014 0.9351197604600984 8 0.1464510972268245 0.7458844351048524 9 0.1251537598619582 0.7170927609898097 >10 0.7057836873335466 8.237373495130667 >50 0.04743660546121839 2.0653545675389533 >100 0.024189819819017203 2.6911552040752196 >500 0.0017008467006765589 0.7139357542134325 >1k 0.0017008467006765589 2.2486449690342183 >5k 5.669489002255197E-4 3.0975716090291594 >10k+ 3.779659334836798E-4 2.8813696288603796 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13727 1.6448168558080238 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10222 1.2248355722349833 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9779 1.1717537723425848 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9016 1.0803284601125622 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6951 0.832892982058831 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4517 0.5412426413407768 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2320 0.27799046444777553 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2249 0.26948299764786515 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2234 0.26768564550703905 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2141 0.256542062233917 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1748 0.20945143614427225 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1244 0.14906040421251412 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1135 0.13599964532251088 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1102 0.1320454706126934 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 836 0.10017242598204326 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.797352140826135E-4 0.0 22-23 0.0 0.0 0.0 0.0014977934506884458 0.0 24-25 0.0 0.0 0.0 0.00275593994926674 0.0 26-27 0.0 0.0 0.0 0.0040140864478450345 0.0 28-29 0.0 0.0 0.0 0.006230820754863935 0.0 30-31 0.0 0.0 0.0 0.012521553247755408 0.0 32-33 0.0 0.0 0.0 0.022227254808216537 0.0 34-35 0.0 0.0 0.0 0.03852324755170683 0.0 36-37 0.0 0.0 0.0 0.06266767797680457 0.0 38-39 0.0 0.0 0.0 0.0912455770159401 0.0 40-41 0.0 0.0 0.0 0.12881023675920633 0.0 42-43 0.0 0.0 0.0 0.16589560259825226 0.0 44-45 0.0 0.0 0.0 0.209870818310465 0.0 46-47 0.0 0.0 0.0 0.26486979381974474 0.0 48-49 0.0 0.0 0.0 0.3257401196557232 0.0 50-51 0.0 0.0 0.0 0.3865505337536741 0.0 52-53 0.0 0.0 0.0 0.4512552108234149 0.0 54-55 0.0 0.0 0.0 0.5251263838113691 0.0 56-57 0.0 0.0 0.0 0.6098415813823075 0.0 58-59 0.0 0.0 0.0 0.6989303358292563 0.0 60-61 0.0 0.0 0.0 0.7920930884620776 0.0 62-63 0.0 0.0 0.0 0.8797439611963656 0.0 64-65 0.0 0.0 0.0 0.9783586819896928 0.0 66-67 0.0 0.0 0.0 1.075954903236552 0.0 68-69 0.0 0.0 0.0 1.1941008506268567 0.0 70-71 0.0 0.0 0.0 1.3480740173576287 0.0 72-73 0.0 0.0 0.0 1.5177440594516158 0.0 74-75 0.0 0.0 0.0 1.7014933599820745 0.0 76-77 0.0 0.0 0.0 1.9156179116924945 0.0 78-79 0.0 0.0 0.0 2.146038456146405 0.0 80-81 0.0 0.0 0.0 2.4069540752563325 0.0 82-83 0.0 0.0 0.0 2.671763957338049 0.0 84-85 0.0 0.0 0.0 2.943044307126741 0.0 86-87 0.0 0.0 0.0 3.235473500439153 0.0 88-89 0.0 0.0 0.0 3.55066915420203 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1120 0.0 56.276863 2 GTACCTG 1285 0.0 49.425373 1 ACCTGGG 1465 0.0 42.038364 3 TATAACG 125 5.7771103E-9 34.121548 2 TATCACG 60 0.0039949943 31.594027 2 CCTGGGA 1445 0.0 29.178452 4 ATAACGC 130 3.1065792E-7 29.153234 3 GTACATG 8105 0.0 28.595922 1 TACATGG 7905 0.0 28.296738 2 ACGCGAT 35 0.008494621 27.075726 78-79 GTACACG 160 6.370465E-8 26.660654 1 ACATGGG 8060 0.0 26.331953 3 TCACGCA 90 9.0662244E-4 26.328354 2 TAACGCA 180 6.884875E-9 26.31889 4 GGTATCA 8580 0.0 26.239443 1 GGGACCG 110 9.6461845E-5 25.840366 7 ATGGGCG 130 1.0225309E-5 25.509079 5 CATGGGA 6140 0.0 25.075817 4 ATGGGAG 1780 0.0 25.017733 5 TGGGATA 760 0.0 23.687002 6 >>END_MODULE