FastQCFastQC Report
Fri 27 May 2016
SRR939530_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939530_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829466
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99761.2027014971077779No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70300.8475332322241057No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56090.676218193391893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51250.6178673990253971No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39960.4817557320010706No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29560.3563738598085997No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15390.18554105894635825No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13180.15889741110545821No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12710.15323111495829847No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12280.1480470567811098No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10940.1318920847870799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG9900.052.9167822
GTACCTG11050.047.9134641
ATAACGC751.2245982E-744.937753
ACCTGGG11900.041.6739733
GTACATG67450.031.9209981
TATAACG1102.1574542E-631.1566142
TACATGG68250.030.9185772
ACATGGG71750.028.8550053
CATGGGT7100.027.5410694
GTATCAA105000.026.1240271
CATGGGA51450.025.8627134
CATGGGG20000.025.5157934
TAACGCA1501.022685E-625.4363024
CCTGGGA12600.024.6035674
GGTATCA77900.024.3377781
ACACGGG2052.3099346E-823.4866313
TGGGAGT6700.023.179726
ACGCAGG3800.022.9890987
ATGGGAG16400.022.5088235
GAGTACT65150.022.37187812-13
Produced by FastQC (version 0.11.5)