FastQCFastQC Report
Fri 27 May 2016
SRR939530_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939530_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829466
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130051.567876199868349No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98421.186546525113748No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95971.1570094494530216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87291.0523638099693056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69900.8427108525243951No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44020.5307028859531313No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22820.2751167618684792No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21610.2605290632768552No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20970.25281325575731856No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19730.2378638786882163No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18650.22484345349899817No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12240.14756481881113873No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10920.13165096580209434No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10550.1271902645798622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG11200.058.4856572
ACCTGGG13450.047.6315773
GTACCTG13800.047.4780851
ATAACGC804.517484E-429.6594773
CCTGGGA12850.026.9654734
GTACACG1806.726623E-926.3767131
GTACATG83750.026.3042771
GGTATCA92400.025.9999031
TACATGG82650.025.7864632
TATAACG950.001231300524.9824312
ACATGGG80750.024.9764023
CCTGGGG8800.024.8120984
CATGGGG20050.023.2006424
GTATCAA122300.022.865571
GTACTTG2955.456968E-1222.5319711
CATGGGA58650.022.3372334
ATGGGGT6550.021.7168435
ATGGGAG15950.021.7009585
TATCACG1100.002888452321.5757372
GAGTACT78950.020.8948412-13
Produced by FastQC (version 0.11.5)