##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939530_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 829466 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.88834864840753 31.0 31.0 31.0 28.0 34.0 2 31.06843439031859 31.0 31.0 34.0 28.0 34.0 3 31.059214000332744 31.0 31.0 34.0 27.0 34.0 4 34.9738446181037 35.0 35.0 37.0 32.0 37.0 5 34.59303334916682 35.0 35.0 37.0 30.0 37.0 6 34.69112055225892 35.0 35.0 37.0 31.0 37.0 7 34.5544868626321 35.0 35.0 37.0 30.0 37.0 8 34.47356250889126 35.0 35.0 37.0 30.0 37.0 9 35.95321930012803 37.0 35.0 39.0 30.0 39.0 10-11 35.741479457868074 37.0 35.0 39.0 30.0 39.0 12-13 35.72740112313223 37.0 35.0 39.0 30.0 39.0 14-15 36.4468459225574 38.0 35.0 40.0 30.0 41.0 16-17 36.40633612468745 38.0 35.0 40.0 30.0 41.0 18-19 36.49277426681745 38.0 35.0 40.0 30.0 41.0 20-21 36.366721481049254 38.0 34.5 40.0 30.0 41.0 22-23 36.11337414674019 38.0 34.0 40.0 29.5 41.0 24-25 35.898736054280704 38.0 34.0 40.0 29.0 41.0 26-27 35.36559545538937 38.0 34.0 40.0 27.0 41.0 28-29 35.115102969862534 37.0 33.0 40.0 27.0 41.0 30-31 34.7234702808795 37.0 33.0 39.5 26.5 40.5 32-33 34.24494554327724 36.5 33.0 39.0 25.0 41.0 34-35 34.215510340387674 37.0 33.0 40.0 24.5 41.0 36-37 33.91795805976375 37.0 33.0 40.0 24.0 41.0 38-39 33.68399789744245 36.0 33.0 40.0 23.0 41.0 40-41 33.49191347204105 36.0 32.5 40.0 22.5 41.0 42-43 33.292174724461276 36.0 32.0 40.0 21.5 41.0 44-45 32.82906954594884 36.0 31.5 39.0 19.0 41.0 46-47 32.29895920990131 36.0 31.0 39.0 15.0 41.0 48-49 31.831247453180723 35.0 30.0 39.0 12.5 40.0 50-51 31.584703291033026 35.0 30.0 39.0 9.0 40.0 52-53 31.468879978202843 35.0 30.0 39.0 9.0 40.0 54-55 31.158632783019435 35.0 29.5 39.0 8.0 40.0 56-57 30.6985819792493 35.0 28.5 38.5 7.0 40.0 58-59 30.293412267651718 35.0 28.0 38.0 3.5 40.0 60-61 29.951636353991603 34.0 27.5 38.0 2.0 40.0 62-63 29.799194300911672 34.0 27.5 38.0 2.0 40.0 64-65 29.380195812727706 34.0 27.0 37.5 2.0 40.0 66-67 28.599846768885044 33.5 25.5 37.0 2.0 39.5 68-69 25.400127913621535 31.0 15.5 35.0 2.0 39.0 70-71 24.180118895771496 29.5 10.5 35.0 2.0 38.0 72-73 25.126545271295026 30.0 16.0 35.0 2.0 37.5 74-75 25.81531431065288 31.0 20.5 35.0 2.0 37.0 76-77 25.415569173420007 30.5 21.0 34.0 2.0 36.0 78-79 26.469220558769138 32.0 23.5 35.0 2.0 36.5 80-81 26.48293480383765 32.0 24.0 35.0 2.0 36.0 82-83 26.16946143663514 32.0 23.5 35.0 2.0 36.0 84-85 25.819916066481326 32.0 22.5 35.0 2.0 35.5 86-87 25.366622019467947 31.5 20.0 34.0 2.0 35.0 88-89 24.874835134894017 31.0 19.0 34.0 2.0 35.0 90-91 24.276882958433497 31.0 16.5 34.0 2.0 35.0 92-93 23.7360542807059 31.0 8.5 34.0 2.0 35.0 94-95 22.944114044457518 30.0 2.0 34.0 2.0 35.0 96-97 22.297930234632886 30.0 2.0 34.0 2.0 35.0 98-99 21.49413116390545 29.0 2.0 34.0 2.0 35.0 100-101 20.25502070006486 27.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.30461660901241316 1101 2 0.3394860975515108 1101 3 0.40566792865539725 1101 4 0.3689771166666773 1101 5 0.6018525505488199 1101 6 0.3419457477494632 1101 7 0.44340664752490255 1101 8 0.5719009323314168 1101 9 0.7715363835221467 1101 10-11 0.7136801062923723 1101 12-13 0.7792823509940803 1101 14-15 0.6590995522880192 1101 16-17 0.5401400077784331 1101 18-19 0.6506208830063684 1101 20-21 0.8067000311190142 1101 22-23 0.8487414555506874 1101 24-25 0.9564364205509932 1101 26-27 0.894683656212429 1101 28-29 0.7536397336385292 1101 30-31 0.5198453474964637 1101 32-33 1.0165967871074884 1101 34-35 0.886683852083145 1101 36-37 1.1726337525013761 1101 38-39 0.8061729548638183 1101 40-41 0.925655741405059 1101 42-43 0.5391045060554376 1101 44-45 0.935100208850109 1101 46-47 0.8479648389048293 1101 48-49 0.9330530777202988 1101 50-51 0.6963141781028099 1101 52-53 0.5271872410379466 1101 54-55 0.6266903359908191 1101 56-57 0.7088102787967081 1101 58-59 0.39940999452954884 1101 60-61 0.4805091482862949 1101 62-63 0.35892160518525884 1101 64-65 0.3497137474010614 1101 66-67 0.22722955159073877 1101 68-69 -7.028497698857834 1101 70-71 -7.4727754013137435 1101 72-73 -4.221269391363503 1101 74-75 -2.271112687458608 1101 76-77 0.12873332276857852 1101 78-79 0.3924664037241996 1101 80-81 0.33739127014136727 1101 82-83 -0.13218667449193688 1101 84-85 0.08868952643328853 1101 86-87 -0.03035731360936822 1101 88-89 -0.4759842191350323 1101 90-91 -0.6608151013673762 1101 92-93 -0.5771086810693475 1101 94-95 -0.3553228118796632 1101 96-97 -0.6203217740187661 1101 98-99 -0.5724163646981424 1101 100-101 -0.7415086039022256 1102 1 0.40849020032892724 1102 2 0.466663503267732 1102 3 0.4500501065992175 1102 4 0.5188625770544206 1102 5 0.6925079631220399 1102 6 0.5836704529444816 1102 7 0.42983485655584985 1102 8 0.5541741977097061 1102 9 0.5312799377862447 1102 10-11 0.6114650331868887 1102 12-13 0.5029113348970427 1102 14-15 0.688495490359756 1102 16-17 0.7823855222842298 1102 18-19 0.5418044077515418 1102 20-21 0.629373108929073 1102 22-23 0.6197929138167595 1102 24-25 0.6922765570760632 1102 26-27 0.5617481366330708 1102 28-29 0.821825395431496 1102 30-31 0.6355418867089853 1102 32-33 0.8323994099877297 1102 34-35 0.7423139736950048 1102 36-37 0.8192329253562534 1102 38-39 1.2106054072414665 1102 40-41 1.2225285793737939 1102 42-43 1.0076676104632938 1102 44-45 1.1568189180117443 1102 46-47 1.3708227944111897 1102 48-49 1.2102946717427123 1102 50-51 1.1898972692886503 1102 52-53 0.73668326674402 1102 54-55 0.5651964012092137 1102 56-57 0.7064003346059415 1102 58-59 0.6111045658131609 1102 60-61 0.5700921933487919 1102 62-63 0.44379622658955853 1102 64-65 0.20802286598415165 1102 66-67 0.5257685949927975 1102 68-69 -5.791129726900824 1102 70-71 -6.414517787120058 1102 72-73 -0.6667285491514292 1102 74-75 0.7742649857729482 1102 76-77 1.214980113353267 1102 78-79 1.0809205801920712 1102 80-81 0.7192809431269289 1102 82-83 0.6475840156668795 1102 84-85 0.21033405389973225 1102 86-87 0.387360580706698 1102 88-89 0.33174297672001885 1102 90-91 0.29182007843674995 1102 92-93 0.6684115901358574 1102 94-95 0.6734120250743523 1102 96-97 0.26316491939943276 1102 98-99 0.33675718203248195 1102 100-101 0.5409199433665002 1103 1 0.30234483141251545 1103 2 0.13689639694788625 1103 3 0.10429932424424493 1103 4 0.053430354547593595 1103 5 0.2726348475488152 1103 6 0.20498713563566184 1103 7 0.21173693504007218 1103 8 0.23609570359298715 1103 9 0.2888371341527076 1103 10-11 0.34541237132386726 1103 12-13 0.22201341783594586 1103 14-15 0.4886067338741569 1103 16-17 0.27586682557809894 1103 18-19 0.2614169239462001 1103 20-21 0.3462849691705969 1103 22-23 0.5473237656401508 1103 24-25 0.5341387660826982 1103 26-27 0.5672663841579322 1103 28-29 0.5648015244789164 1103 30-31 0.7183131247540189 1103 32-33 0.6396239276128384 1103 34-35 0.43209800783773034 1103 36-37 0.5224107281297918 1103 38-39 0.5915333934616314 1103 40-41 0.7340662270746137 1103 42-43 0.5917455576430442 1103 44-45 0.4851988832899039 1103 46-47 0.47441537834188807 1103 48-49 0.6815786248348239 1103 50-51 0.476818298767558 1103 52-53 0.09822654467832947 1103 54-55 -0.10899683582856667 1103 56-57 0.30413809441646933 1103 58-59 0.4917448532891733 1103 60-61 0.42691659317208774 1103 62-63 0.12983832093742365 1103 64-65 0.12357307591143041 1103 66-67 0.12498382706359479 1103 68-69 -6.950633622898657 1103 70-71 -7.515176462567414 1103 72-73 -1.1697094793649967 1103 74-75 0.23369265707125564 1103 76-77 0.523641268578185 1103 78-79 0.19631005004681157 1103 80-81 -0.001846167746599292 1103 82-83 -0.2676481788743921 1103 84-85 -0.4201716648088514 1103 86-87 -0.10626426260575883 1103 88-89 -0.3849343935773426 1103 90-91 -0.5576738811270374 1103 92-93 -0.693498581035616 1103 94-95 -0.42840734466122754 1103 96-97 -0.11721365502544501 1103 98-99 -0.21756281569067681 1103 100-101 -0.33037601264095784 1104 1 0.2358190443244368 1104 2 0.2516017748726682 1104 3 0.046413742962855054 1104 4 0.33439568592086033 1104 5 0.199006279620356 1104 6 0.1348376807783822 1104 7 0.381686708407706 1104 8 0.5567106735795164 1104 9 0.48585906236537113 1104 10-11 0.3771047638266154 1104 12-13 0.32062177260747404 1104 14-15 0.5563293848603195 1104 16-17 0.6267457916444954 1104 18-19 0.26247780842494706 1104 20-21 0.3412586308145933 1104 22-23 0.5080869654441429 1104 24-25 0.461451641251152 1104 26-27 0.7349804598653975 1104 28-29 0.7377569330297007 1104 30-31 0.635553110053543 1104 32-33 0.6658281417497989 1104 34-35 0.7250019647163342 1104 36-37 0.9678468423035085 1104 38-39 0.8927939594300298 1104 40-41 1.1426800945253106 1104 42-43 0.8734195745485849 1104 44-45 1.1051915330510198 1104 46-47 0.8540607293157905 1104 48-49 0.8817593182073615 1104 50-51 1.0718164612078382 1104 52-53 0.857917834645253 1104 54-55 0.9889506099634211 1104 56-57 1.081215149420757 1104 58-59 1.0224008621094605 1104 60-61 0.9889642472208422 1104 62-63 0.751585227711896 1104 64-65 0.48160872457001247 1104 66-67 0.2182265074507086 1104 68-69 -6.210506831277929 1104 70-71 -4.180050895986501 1104 72-73 0.26901219158931156 1104 74-75 0.8593211024957306 1104 76-77 0.8184593501658384 1104 78-79 0.39220004147153276 1104 80-81 0.18774895659494462 1104 82-83 0.12285898760852021 1104 84-85 0.12548556905124997 1104 86-87 0.10430783098728114 1104 88-89 0.353566770210481 1104 90-91 0.09682569010902853 1104 92-93 0.09012876185302687 1104 94-95 0.12819316985549634 1104 96-97 0.0054544816889965375 1104 98-99 0.14527008887872128 1104 100-101 0.5497471038603265 1105 1 0.133579499657273 1105 2 0.20993387088850568 1105 3 0.14568125100541707 1105 4 0.1490108801797092 1105 5 0.27973134767282204 1105 6 0.10425366692042104 1105 7 0.13308487717780793 1105 8 0.49985346001851383 1105 9 0.21611642440446843 1105 10-11 0.25357469701148005 1105 12-13 0.28385364590617 1105 14-15 0.2398879099778597 1105 16-17 0.22855846177564842 1105 18-19 0.36427934269863727 1105 20-21 0.2418921373723819 1105 22-23 0.3651223527245193 1105 24-25 0.25609776844738263 1105 26-27 0.12365900478002345 1105 28-29 0.23445453263375526 1105 30-31 0.5944098286875388 1105 32-33 0.7818056225713832 1105 34-35 0.9168294826574339 1105 36-37 0.7741658227538863 1105 38-39 0.7791265503728582 1105 40-41 0.6672148631469312 1105 42-43 0.7176697601462152 1105 44-45 0.8364461729916357 1105 46-47 0.7951483313944863 1105 48-49 0.6642327158812016 1105 50-51 0.6787726601684909 1105 52-53 0.32722741148267076 1105 54-55 0.5764747376545891 1105 56-57 0.7752364312816802 1105 58-59 0.7737705725771669 1105 60-61 0.6832936211381906 1105 62-63 0.6077495434753182 1105 64-65 0.534500335557393 1105 66-67 0.7198399694650632 1105 68-69 -2.240718412569688 1105 70-71 -2.235584179827132 1105 72-73 1.2799636887434822 1105 74-75 1.5121830178632152 1105 76-77 1.316120820099023 1105 78-79 1.0241352060347602 1105 80-81 0.7424618989359502 1105 82-83 0.7518567604370254 1105 84-85 0.9954063807315272 1105 86-87 0.7721375760998797 1105 88-89 0.7235383119080758 1105 90-91 0.7289068581367459 1105 92-93 0.8607300981559227 1105 94-95 1.0680075722311457 1105 96-97 1.0570803168783094 1105 98-99 0.6993074558670891 1105 100-101 0.4911316621379811 1106 1 0.09551330234084077 1106 2 0.30053540649086585 1106 3 0.131902184424451 1106 4 0.12698330709908845 1106 5 0.25770128334891496 1106 6 0.05274222962252395 1106 7 0.2757210111668371 1106 8 0.2052863633632569 1106 9 0.1506390772796138 1106 10-11 0.3253262403366861 1106 12-13 0.14103191429278894 1106 14-15 0.3656209583129666 1106 16-17 0.20480321148043856 1106 18-19 0.19173209626983123 1106 20-21 0.10222805661624079 1106 22-23 0.24226295351274985 1106 24-25 0.16422196190737992 1106 26-27 0.3016471265320675 1106 28-29 0.09413650051664746 1106 30-31 0.1027343181072311 1106 32-33 0.4957423848519653 1106 34-35 0.22196318768426693 1106 36-37 0.28241432925354104 1106 38-39 0.4255456372823758 1106 40-41 0.3318262541077033 1106 42-43 0.45944865732263906 1106 44-45 0.5503816896505782 1106 46-47 0.33481017003167324 1106 48-49 0.2531053286473295 1106 50-51 0.0630245148991122 1106 52-53 -0.03322310495206082 1106 54-55 -0.05882343925357958 1106 56-57 0.20111820684059367 1106 58-59 0.38447021333988474 1106 60-61 0.5857464992715506 1106 62-63 0.18727501145536962 1106 64-65 0.1560941831829865 1106 66-67 0.6928872084201352 1106 68-69 -2.992262983403208 1106 70-71 -2.7456064515420557 1106 72-73 0.6616333698145169 1106 74-75 1.50452244477761 1106 76-77 1.3108530861837373 1106 78-79 0.8457048886005438 1106 80-81 0.6098928790408777 1106 82-83 0.4019641330223891 1106 84-85 0.4807055541370069 1106 86-87 0.6637410897494007 1106 88-89 0.6882461139837837 1106 90-91 0.7040143552842721 1106 92-93 0.7690996790789804 1106 94-95 0.9188643107950938 1106 96-97 0.5715394928746811 1106 98-99 0.8679509240763359 1106 100-101 0.9592437481556289 1107 1 0.021004229509621553 1107 2 0.057712885983779216 1107 3 0.03433040962952205 1107 4 -0.20213209185691738 1107 5 -0.14145668334260364 1107 6 -0.02298639329569596 1107 7 -0.013452180481188236 1107 8 -0.10421525234641393 1107 9 0.020442395698701432 1107 10-11 -0.1683119028400526 1107 12-13 0.13390880964451668 1107 14-15 -0.15369376328782636 1107 16-17 -0.020592171318469354 1107 18-19 0.17164447509161107 1107 20-21 0.09975400118496225 1107 22-23 0.1714202987774769 1107 24-25 0.025294233843737857 1107 26-27 0.1714387931987318 1107 28-29 0.08859026636303469 1107 30-31 0.1378216285720626 1107 32-33 0.034647586194239466 1107 34-35 -0.03865544269107346 1107 36-37 0.22460610156276317 1107 38-39 0.09207636683743203 1107 40-41 -0.0759773128820953 1107 42-43 -0.12604801804400267 1107 44-45 0.057737829347317415 1107 46-47 0.22413017376023703 1107 48-49 0.03500005894810343 1107 50-51 -0.17894742768105232 1107 52-53 -0.5692580912806697 1107 54-55 -0.5582253159625026 1107 56-57 -0.048807998727394164 1107 58-59 0.1302017880353823 1107 60-61 -0.0918922342606372 1107 62-63 -0.46943329080662366 1107 64-65 -0.6417246087633224 1107 66-67 -0.3823753444011402 1107 68-69 -2.982891090537187 1107 70-71 -2.808685155245758 1107 72-73 0.4993594138115327 1107 74-75 0.553024806199435 1107 76-77 -0.06730453872304665 1107 78-79 -0.15687403260253774 1107 80-81 -0.10405659896061081 1107 82-83 -0.09343730868777911 1107 84-85 -0.3202320235413403 1107 86-87 -0.3953449467904946 1107 88-89 -0.2821739705302626 1107 90-91 -0.5507900506317114 1107 92-93 -0.5117230899988279 1107 94-95 -0.5238207190333917 1107 96-97 -0.35915199979248413 1107 98-99 0.07693675554538615 1107 100-101 0.17826562237884502 1108 1 -0.09398576048036844 1108 2 -0.09581564254474983 1108 3 -0.07537304382393373 1108 4 -0.024673383148204664 1108 5 0.09421648983056485 1108 6 -0.09127343033273405 1108 7 0.1525575792785716 1108 8 0.1024914543602975 1108 9 0.20390711041341802 1108 10-11 0.18106872779058136 1108 12-13 -0.15247132048562406 1108 14-15 0.222448003478938 1108 16-17 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0.12089799262225753 2316 94-95 0.2153726570349832 2316 96-97 0.69083909707361 2316 98-99 0.7824923111009419 2316 100-101 0.5942342833475074 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 14.0 4 57.0 5 159.0 6 497.0 7 1058.0 8 1717.0 9 2540.0 10 3859.0 11 5641.0 12 7255.0 13 8568.0 14 9302.0 15 11122.0 16 12890.0 17 13959.0 18 12306.0 19 10119.0 20 9846.0 21 9935.0 22 10443.0 23 11937.0 24 13944.0 25 16818.0 26 20715.0 27 25401.0 28 30612.0 29 36355.0 30 42043.0 31 49074.0 32 56635.0 33 65662.0 34 76645.0 35 83997.0 36 77185.0 37 62140.0 38 27171.0 39 1844.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.543176975495406 20.0866300964821 14.459412039219895 18.910780888802602 2 15.610356661385117 23.74659653539965 40.481754535742105 20.161292267473126 3 18.90589189847383 32.07359960263352 30.19430690532768 18.826201593564978 4 11.746311815706644 19.10916349663043 37.08145866723309 32.06306602042984 5 11.128605633021728 40.287245046813254 34.02622892318672 14.557920396978297 6 25.364993863521835 37.07529904782113 21.708183337231425 15.851523751425617 7 24.768827173145134 35.429903094279936 23.564317283649963 16.23695244892497 8 25.546074221245956 33.371711438443526 22.507613331950918 18.574601008359597 9 27.00930478163059 17.605061569732815 23.063874830312514 32.32175881832408 10-11 23.046010030462753 28.61949411212198 28.6049047865648 19.729591070850468 12-13 25.36601861920802 26.897485852343557 29.539366291083663 18.197129237364763 14-15 22.001263463481322 27.430419088907804 30.33554117950585 20.23277626810502 16-17 18.646615906826298 30.510216568219263 30.910054294262 19.93311323069244 18-19 18.205069780002507 28.36114647531418 35.13907776588255 18.294705978800767 20-21 19.96067349354886 27.26458950698401 34.834640600096925 17.940096399370198 22-23 19.23478478924995 27.659361565151556 34.48158212633189 18.624271519266614 24-25 18.733946159767697 27.93761540228264 35.196769369046784 18.131669068902887 26-27 18.978990130129418 27.666803103526643 35.262920259185826 18.091286507158113 28-29 18.234694861717262 28.126311093964745 35.48628023051142 18.152713813806574 30-31 18.403428716100137 28.432242469543624 35.13921624179441 18.025112572561834 32-33 18.322451762320206 28.55072664701936 34.68616046213171 18.440661128528724 34-35 18.777560026982076 28.454335729127923 34.52952264752282 18.238581596367183 36-37 19.07704207472765 28.189452116285917 34.669597304746134 18.063908504240295 38-39 18.887420575297334 28.397929074419366 34.57775927300375 18.136891077279557 40-41 18.62685832395197 28.636559435598013 34.664943633404164 18.071638607045852 42-43 18.371986388976037 29.10575089895504 33.904119506721045 18.618143205347877 44-45 18.588559382877083 29.242931213189998 33.41753655208031 18.750972851852612 46-47 18.90223338475526 29.272971996266733 32.65728684083356 19.167507778144454 48-49 19.024389949620208 29.399997224918543 32.471469447861345 19.104143377599904 50-51 19.19431036594231 29.858935540365017 32.256844338026234 18.689909755666438 52-53 18.939508635462428 30.043676445662605 32.139916628113255 18.87689829076172 54-55 18.640450075209554 30.064703136365118 32.06170681662741 19.233139971797925 56-57 19.11035725720722 30.359717349004278 31.443687173676967 19.086238220111536 58-59 19.035319213847686 29.880456468536842 31.73230497076076 19.35191934685471 60-61 19.04922098772354 29.851697380423136 32.07566393742581 19.023417694427515 62-63 19.350459582758653 29.737910031731946 32.05897174988667 18.852658635622728 64-65 19.037663587966605 29.926688718526517 32.03964029787493 18.99600739563195 66-67 18.646262663705237 30.17356241551744 31.90496055619181 19.275214364585516 68-69 19.293084314587315 30.047984108906444 31.49533879063533 19.163592785870915 70-71 19.05474457880554 30.053883608487126 31.530423383370138 19.3609484293372 72-73 19.09330613711467 30.082127015293636 31.046032140655743 19.778534706935943 74-75 18.821961347208692 30.532146831152616 30.984473145386726 19.661418676251962 76-77 18.416459410131193 30.406858973352385 31.649552652017988 19.527128964498434 78-79 18.682145492603826 30.291928117026867 31.391587126272686 19.634339264096614 80-81 18.48165759231485 30.819380211322283 31.047484821157305 19.65147737520556 82-83 18.658832550283382 30.874110403412384 30.62490882501516 19.842148221289072 84-85 18.559104770734336 30.594233501534422 30.682121636455374 20.164540091275867 86-87 18.689375841301015 30.44833017760682 30.847211597641554 20.01508238345061 88-89 18.823142201641303 30.19797145864252 30.745795773892265 20.233090565823915 90-91 18.50953206239168 30.488011453545322 30.92589857584206 20.07655790822093 92-93 18.381099871177135 30.69890473437593 30.74905944840665 20.17093594604028 94-95 18.15894992933052 30.759954805313573 30.565953054713198 20.515142210642708 96-97 18.18845670283683 30.46952872256711 30.458131955269195 20.88388261932687 98-99 18.384504224032582 30.340991232411923 30.26834498587166 21.00615955768383 100-101 18.29342770414269 30.280791340232287 30.65692814575226 20.76885280987276 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7936.0 1 5458.5 2 6546.5 3 8832.0 4 6300.0 5 4933.0 6 5394.5 7 7289.0 8 8700.5 9 8485.5 10 6946.0 11 5158.5 12 4369.0 13 3994.0 14 3833.5 15 3653.0 16 3408.0 17 3340.0 18 3364.5 19 3455.5 20 3591.5 21 3861.0 22 4324.5 23 4758.0 24 5320.0 25 6208.5 26 7371.5 27 8631.0 28 9663.0 29 10791.0 30 12157.0 31 13697.0 32 15408.5 33 16740.0 34 18162.5 35 19724.5 36 21203.0 37 22415.0 38 22815.5 39 22803.5 40 22593.0 41 22905.5 42 23416.0 43 23680.0 44 24129.0 45 24318.0 46 24846.0 47 26645.0 48 30875.0 49 30732.5 50 26077.0 51 23151.5 52 20817.0 53 19454.5 54 18493.0 55 17023.0 56 15166.5 57 13470.5 58 12123.5 59 10831.0 60 9418.0 61 7935.5 62 6361.0 63 4724.0 64 3313.5 65 2399.5 66 1755.0 67 1341.5 68 1058.0 69 757.0 70 569.0 71 456.0 72 392.0 73 341.0 74 250.5 75 193.5 76 140.0 77 93.0 78 56.5 79 31.0 80 23.0 81 13.0 82 9.5 83 6.0 84 2.5 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023267982051102757 2 0.019771756768812706 3 6.02797462463802E-4 4 0.10536899643867259 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011151753055580338 12-13 0.0 14-15 0.0 16-17 5.425177162174218E-4 18-19 2.411189849855208E-4 20-21 0.0 22-23 0.0 24-25 0.003677064521029192 26-27 0.002712588581087109 28-29 7.233569549565624E-4 30-31 1.205594924927604E-4 32-33 6.630772087101821E-4 34-35 0.0031948265510581505 36-37 0.0904196193695703 38-39 0.1025961281113391 40-41 0.08378884728246848 42-43 0.08752619154974406 44-45 0.08644115611730921 46-47 0.08457248398367143 48-49 0.07975010428396101 50-51 0.07962954479146825 52-53 0.06383625127491663 54-55 0.05413121212924942 56-57 0.029597355406972677 58-59 0.022484345349899817 60-61 0.013804061890421064 62-63 0.0021700708648696872 64-65 0.006932170818333723 66-67 0.15220635927211 68-69 0.7064786260075759 70-71 0.8605536574133238 72-73 0.34727161812539636 74-75 0.05045414760822023 76-77 0.19132791458601076 78-79 0.014226020114145728 80-81 0.0026523088348407286 82-83 0.002049511372376927 84-85 3.01398731231901E-4 86-87 0.0025920290885943485 88-89 3.616784774782812E-4 90-91 0.0034359455360436714 92-93 0.003918183506014713 94-95 0.07323989168935194 96-97 0.03417861612169758 98-99 0.012598466965493464 100-101 0.013683502397928305 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 829466.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.51048831093769 #Duplication Level Percentage of deduplicated Percentage of total 1 85.73876596359814 55.3104965948892 2 9.263634789700038 11.9520320763548 3 2.1748460187275995 4.209011360076486 4 0.857974623820696 2.213934477642647 5 0.4677804317773109 1.50883720381278 6 0.27777996915830055 1.0751832872039508 7 0.20598198434675802 0.9301598875425698 8 0.1426559945341517 0.7362246294304468 9 0.10987740868575396 0.6379420759792609 >10 0.68779237183614 8.164912626137761 >50 0.04742055873621002 2.1228697488458614 >100 0.021366521327766418 2.501248334480852 >500 0.0014994050004016094 0.6191759411972647 >1k 0.0016868306254518106 2.1946226339917114 >5k 7.497025002008047E-4 4.250925159268392 >10k+ 1.8742562505020117E-4 1.5724239631459538 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13005 1.567876199868349 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9842 1.186546525113748 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9597 1.1570094494530216 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8729 1.0523638099693056 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6990 0.8427108525243951 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4402 0.5307028859531313 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2282 0.2751167618684792 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2161 0.2605290632768552 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2097 0.25281325575731856 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1973 0.2378638786882163 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1865 0.22484345349899817 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1224 0.14756481881113873 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1092 0.13165096580209434 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1055 0.1271902645798622 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.0279746246380204E-5 0.0 22-23 0.0 0.0 0.0 0.001506993656159505 0.0 24-25 0.0 0.0 0.0 0.0030742670585653903 0.0 26-27 0.0 0.0 0.0 0.004159302491000234 0.0 28-29 0.0 0.0 0.0 0.006630772087101822 0.0 30-31 0.0 0.0 0.0 0.012297068234261561 0.0 32-33 0.0 0.0 0.0 0.02350910103608828 0.0 34-35 0.0 0.0 0.0 0.04086966795504578 0.0 36-37 0.0 0.0 0.0 0.0648610069611051 0.0 38-39 0.0 0.0 0.0 0.09584479653174452 0.0 40-41 0.0 0.0 0.0 0.1385831366204281 0.0 42-43 0.0 0.0 0.0 0.17704161472561863 0.0 44-45 0.0 0.0 0.0 0.22092526999298345 0.0 46-47 0.0 0.0 0.0 0.27535788085346474 0.0 48-49 0.0 0.0 0.0 0.3352156688761203 0.0 50-51 0.0 0.0 0.0 0.4032715023882836 0.0 52-53 0.0 0.0 0.0 0.4728343295566063 0.0 54-55 0.0 0.0 0.0 0.5467372984546683 0.0 56-57 0.0 0.0 0.0 0.6343840494969052 0.0 58-59 0.0 0.0 0.0 0.7273354182088234 1.2055949249276041E-4 60-61 0.0 0.0 0.0 0.8229993755018289 1.2055949249276041E-4 62-63 0.0 0.0 0.0 0.9176385771086458 1.2055949249276041E-4 64-65 0.0 0.0 0.0 1.0139656116103615 1.2055949249276041E-4 66-67 0.0 0.0 0.0 1.1183098523628456 1.2055949249276041E-4 68-69 0.0 0.0 0.0 1.2340469651558954 1.2055949249276041E-4 70-71 0.0 0.0 0.0 1.386976681382962 1.2055949249276041E-4 72-73 0.0 0.0 0.0 1.5555791316340875 1.2055949249276041E-4 74-75 0.0 0.0 0.0 1.7418435475354022 1.8083923873914062E-4 76-77 0.0 0.0 0.0 1.9510142670103416 2.4111898498552082E-4 78-79 0.0 0.0 0.0 2.183874926760108 2.4111898498552082E-4 80-81 0.0 0.0 0.0 2.4406666457696877 2.4111898498552082E-4 82-83 0.0 0.0 0.0 2.713191378549573 2.4111898498552082E-4 84-85 0.0 0.0 0.0 2.9998818516973573 2.4111898498552082E-4 86-87 0.0 0.0 0.0 3.2993516310493742 2.4111898498552082E-4 88-89 0.0 0.0 0.0 3.618050649453986 2.4111898498552082E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1120 0.0 58.485657 2 ACCTGGG 1345 0.0 47.631577 3 GTACCTG 1380 0.0 47.478085 1 ATAACGC 80 4.517484E-4 29.659477 3 CCTGGGA 1285 0.0 26.965473 4 GTACACG 180 6.726623E-9 26.376713 1 GTACATG 8375 0.0 26.304277 1 GGTATCA 9240 0.0 25.999903 1 TACATGG 8265 0.0 25.786463 2 TATAACG 95 0.0012313005 24.982431 2 ACATGGG 8075 0.0 24.976402 3 CCTGGGG 880 0.0 24.812098 4 CATGGGG 2005 0.0 23.200642 4 GTATCAA 12230 0.0 22.86557 1 GTACTTG 295 5.456968E-12 22.531971 1 CATGGGA 5865 0.0 22.337233 4 ATGGGGT 655 0.0 21.716843 5 ATGGGAG 1595 0.0 21.700958 5 TATCACG 110 0.0028884523 21.575737 2 GAGTACT 7895 0.0 20.89484 12-13 >>END_MODULE