FastQCFastQC Report
Fri 27 May 2016
SRR939529_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939529_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636162
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74140.4531336139086472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60740.3712346332453632No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38220.23359545081721736No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21620.13213850462240292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58350.042.9708141
GTATCAA99850.038.4405061
TATCAAC124550.030.9001522
ATCAACG124600.030.661933
TCAACGC127400.030.0783254
CAACGCA129450.029.8525165
AACGCAG132800.029.2267956
ACGCAGA146800.026.2538497
GTACATG86400.025.9047281
CGCAGAG147100.025.8866188
TACATGG88300.024.4962482
GCAGAGT148100.024.1851169
GAGTACT80100.023.56747412-13
ACATGGG90800.023.1996523
GTACTTT85250.022.16651714-15
AGTACTT83000.021.41801812-13
CATGGGG38800.020.7141974
AGAGTAC134250.020.37430410-11
CAGAGTA146100.020.17864810-11
CATGGGA53150.020.1620794
Produced by FastQC (version 0.11.5)