FastQCFastQC Report
Fri 27 May 2016
SRR939529_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939529_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636162
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102990.6294608969038518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94600.5781823560258703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61730.37728537883168045No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33840.20682548549593502No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26880.16428691046485616No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19450.11887575924633381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61200.044.9146461
GTATCAA98950.035.7301141
TATAACG1005.042887E-528.4260542
ATCAACG124100.028.1298983
TATCAAC125100.028.062492
TCAACGC125600.027.8316614
CAACGCA128750.027.0771525
AACGCAG130650.026.6833786
GTACATG94150.024.5143281
TACATGG96750.023.5047472
ACGCAGA149300.023.1915637
ATAACGC1852.612942E-723.043223
GAGTACT87550.023.02055512-13
CGCAGAG150950.022.938068
ACATGGG97000.022.1695333
GTACTTT93200.021.57417314-15
GCAGAGT158700.021.2806579
TAAGGTG8650.020.808465
GTGGTAT35450.020.5880811
AGTACTT90350.020.47223912-13
Produced by FastQC (version 0.11.5)