##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939529_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1636162 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.958582340868446 31.0 31.0 31.0 30.0 34.0 2 31.220745867463002 31.0 31.0 34.0 30.0 34.0 3 31.19739304543193 31.0 31.0 34.0 28.0 34.0 4 35.14338433480303 35.0 35.0 37.0 33.0 37.0 5 34.76141115610801 35.0 35.0 37.0 32.0 37.0 6 34.7329237569385 35.0 35.0 37.0 32.0 37.0 7 34.60094110485392 35.0 35.0 37.0 30.0 37.0 8 34.61500817156247 35.0 35.0 37.0 30.0 37.0 9 35.95925403474717 37.0 35.0 39.0 30.0 39.0 10-11 35.824530211556066 37.0 35.0 39.0 30.0 39.0 12-13 35.775832099755405 37.0 35.0 39.0 30.0 39.0 14-15 36.61327973635862 38.0 35.5 40.0 30.0 41.0 16-17 36.61718919031245 38.0 35.0 40.0 30.5 41.0 18-19 36.608851690725004 38.0 35.0 40.0 30.0 41.0 20-21 36.51698456509808 38.0 35.0 40.0 30.0 41.0 22-23 36.37408795706048 38.0 35.0 40.0 30.0 41.0 24-25 36.1404665308203 38.0 34.0 40.0 29.5 41.0 26-27 35.75776267875675 38.0 34.0 40.0 27.5 41.0 28-29 35.469172368017354 38.0 34.0 40.0 27.0 41.0 30-31 35.21639483131866 38.0 34.0 40.0 27.0 41.0 32-33 34.9746116215876 37.0 33.0 40.0 27.0 41.0 34-35 34.90640230001675 37.5 33.0 40.0 26.0 41.0 36-37 34.868852534162265 38.0 33.0 40.0 25.5 41.0 38-39 34.81104377194923 38.0 33.0 40.0 25.0 41.0 40-41 34.56775979395683 37.5 33.0 40.0 25.0 41.0 42-43 34.23406942588815 37.0 33.0 40.0 24.0 41.0 44-45 33.998652945124014 37.0 33.0 40.0 24.0 41.0 46-47 33.61018713305895 37.0 32.5 40.0 22.5 41.0 48-49 33.2458586007987 37.0 32.0 40.0 21.0 41.0 50-51 32.959182525935695 37.0 31.0 40.0 19.5 41.0 52-53 32.58031509104844 36.0 31.0 39.0 18.0 41.0 54-55 32.34057905024075 36.0 31.0 39.0 16.5 40.0 56-57 31.939560691422976 35.5 30.0 39.0 14.0 40.0 58-59 31.499040742909322 35.0 29.5 39.0 11.5 40.0 60-61 31.202024310551153 35.0 29.0 38.5 9.0 40.0 62-63 30.812059258190814 35.0 29.0 38.0 8.0 40.0 64-65 30.366552945246255 34.0 28.0 38.0 7.0 40.0 66-67 28.639752359485186 33.0 25.0 37.0 4.0 39.5 68-69 24.92992228153447 29.5 14.0 35.0 2.0 38.0 70-71 24.314543425406534 28.5 11.0 34.5 2.0 38.0 72-73 26.17682845586195 31.0 21.0 35.0 2.0 38.0 74-75 26.79998710396648 31.0 22.5 35.0 2.0 37.5 76-77 26.88926004882157 31.5 25.0 34.5 2.0 36.0 78-79 27.727819433527976 32.5 26.0 35.0 2.0 37.0 80-81 27.597445424108372 33.0 26.0 35.0 2.0 36.5 82-83 27.184878392237444 32.5 25.5 35.0 2.0 36.0 84-85 26.851914724825537 32.0 25.0 35.0 2.0 36.0 86-87 26.409780021782687 32.0 24.5 34.5 2.0 35.0 88-89 25.91512973654198 32.0 23.5 34.0 2.0 35.0 90-91 25.463579706654965 31.0 21.0 34.0 2.0 35.0 92-93 24.891810224170953 31.0 19.0 34.0 2.0 35.0 94-95 23.494943654723677 30.5 7.0 34.0 2.0 35.0 96-97 22.687408398434876 30.0 2.0 34.0 2.0 35.0 98-99 22.362344009945225 30.0 2.0 34.0 2.0 35.0 100-101 21.104491181191108 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.33719003515770396 1101 2 0.2256714555286905 1101 3 0.29758451514458883 1101 4 0.15263397672483592 1101 5 0.39734458104102544 1101 6 0.4408749476760008 1101 7 0.566548648567327 1101 8 0.45208594470714303 1101 9 0.6573361839471161 1101 10-11 0.6108118931655824 1101 12-13 0.5901271414616076 1101 14-15 0.6572807653129544 1101 16-17 0.6599736906306859 1101 18-19 0.6889336600207159 1101 20-21 0.5653587222589707 1101 22-23 0.6580318193428241 1101 24-25 0.6466888258626042 1101 26-27 0.6833312965981406 1101 28-29 0.7059739611214937 1101 30-31 0.7361340081967072 1101 32-33 0.7222537492444729 1101 34-35 0.8284003602454035 1101 36-37 0.8141626072082389 1101 38-39 0.6419061391956475 1101 40-41 0.7803159901368915 1101 42-43 0.6726903207594077 1101 44-45 0.419959391443939 1101 46-47 0.3917856824711734 1101 48-49 0.3860391518264663 1101 50-51 0.30224383174353164 1101 52-53 0.29314268863644344 1101 54-55 0.26482168172089615 1101 56-57 0.16355941150593978 1101 58-59 0.13726032788371256 1101 60-61 -0.08388193917003761 1101 62-63 -0.18531048445583664 1101 64-65 -0.1899416173441466 1101 66-67 -3.1747091509597105 1101 68-69 -7.811327033853626 1101 70-71 -11.36487467932712 1101 72-73 -11.419124062159792 1101 74-75 -10.149704585671731 1101 76-77 -5.198476960702855 1101 78-79 -2.0366296447886647 1101 80-81 -1.4503129005519 1101 82-83 -1.1780317555741284 1101 84-85 -1.2995137156166372 1101 86-87 -1.1029560652370485 1101 88-89 -0.9223387804047363 1101 90-91 -1.2238063225448172 1101 92-93 -1.6993958391180328 1101 94-95 -5.082864507141579 1101 96-97 -2.065117893031765 1101 98-99 -1.2964363827429857 1101 100-101 -1.0977143264479565 1102 1 0.44297416937871503 1102 2 0.30993291366846876 1102 3 0.4482795040637413 1102 4 0.19951457096642145 1102 5 0.44063409706406276 1102 6 0.476832183310556 1102 7 0.5312111699245818 1102 8 0.6226584778023962 1102 9 0.6941856657079057 1102 10-11 0.6278673903081327 1102 12-13 0.7294509491941881 1102 14-15 0.8224006831923205 1102 16-17 0.8027526152673303 1102 18-19 0.8114920214149564 1102 20-21 0.9193017136798147 1102 22-23 0.8410680394370047 1102 24-25 0.7588600260837666 1102 26-27 0.5905311846566548 1102 28-29 0.5353175852156582 1102 30-31 0.3476214363071719 1102 32-33 0.31667151757545753 1102 34-35 0.5378297872591062 1102 36-37 0.7480978714943731 1102 38-39 0.6332737287375139 1102 40-41 0.47919340288371615 1102 42-43 0.6900792063751524 1102 44-45 0.6700630619462231 1102 46-47 0.5303432915461386 1102 48-49 0.4238075054709114 1102 50-51 0.4351944528601308 1102 52-53 0.505399827738465 1102 54-55 0.3246871480002156 1102 56-57 0.25013419476094256 1102 58-59 0.09587625303247549 1102 60-61 0.23085490770579753 1102 62-63 0.13921586031486655 1102 64-65 0.0989305722221836 1102 66-67 -2.7666291966427146 1102 68-69 -7.7875524985014195 1102 70-71 -11.179886564612026 1102 72-73 -9.34150006544099 1102 74-75 -7.73332674238074 1102 76-77 -4.141875132476379 1102 78-79 -1.28662454623937 1102 80-81 -0.5455897744387173 1102 82-83 -0.457022673358324 1102 84-85 -0.3353831429022627 1102 86-87 -0.2333525964107146 1102 88-89 -0.08687321487364841 1102 90-91 -0.12622207185028245 1102 92-93 -0.5825237764489337 1102 94-95 -4.169761348760247 1102 96-97 -1.130650113597273 1102 98-99 -0.15148632586583588 1102 100-101 0.02638181406353013 1103 1 0.45435752924912975 1103 2 0.30734265172926456 1103 3 0.45033295559467135 1103 4 0.32730468857921124 1103 5 0.5801992861504246 1103 6 0.4529813603299928 1103 7 0.5739248375699759 1103 8 0.42654116734943415 1103 9 0.7163686799893796 1103 10-11 0.7601528123470445 1103 12-13 0.582185041013588 1103 14-15 0.8060417584020527 1103 16-17 0.731920123286379 1103 18-19 0.7245706649673238 1103 20-21 1.0375531020491806 1103 22-23 0.9141887340575394 1103 24-25 0.9819833688954205 1103 26-27 0.7794098301951777 1103 28-29 0.9711052951813457 1103 30-31 0.9586672621510886 1103 32-33 1.0160775503755133 1103 34-35 1.1472433894721092 1103 36-37 0.9132338285230617 1103 38-39 1.0594002306204828 1103 40-41 1.2013137433086811 1103 42-43 0.9954302627275595 1103 44-45 0.8245190848707296 1103 46-47 0.7999432160726982 1103 48-49 0.7359462066876432 1103 50-51 0.8621606520107221 1103 52-53 0.7559736024811201 1103 54-55 0.7298035635852642 1103 56-57 0.6776421696781618 1103 58-59 0.857147616486774 1103 60-61 0.821039332149045 1103 62-63 0.7641434488389294 1103 64-65 0.711681446952813 1103 66-67 -2.332316749813426 1103 68-69 -7.2143843571233255 1103 70-71 -10.995258265821036 1103 72-73 -8.773687063043273 1103 74-75 -6.8419549904147345 1103 76-77 -3.35730327325863 1103 78-79 -0.5893903060109196 1103 80-81 0.0791590611847468 1103 82-83 0.2698881344613433 1103 84-85 0.3114783644260548 1103 86-87 0.5389186909080941 1103 88-89 0.49883135292500924 1103 90-91 0.44871594392631664 1103 92-93 0.4547801332742374 1103 94-95 1.2878402838495795 1103 96-97 1.5720137459868049 1103 98-99 1.4170218267008678 1103 100-101 1.0701175179692797 1104 1 0.5269853648914271 1104 2 0.27441043748448024 1104 3 0.38511739621893426 1104 4 0.10806108457475716 1104 5 0.39540978940320315 1104 6 0.5066740154687039 1104 7 0.6065435721909367 1104 8 0.4870275663786572 1104 9 0.6735517453481918 1104 10-11 0.7980661503994284 1104 12-13 0.6342218158812898 1104 14-15 0.7646472796883259 1104 16-17 0.8009648531208811 1104 18-19 0.9253762738930718 1104 20-21 0.8510133440689316 1104 22-23 0.7303851812884687 1104 24-25 0.7491295245565865 1104 26-27 0.998744444412992 1104 28-29 0.9466530418358801 1104 30-31 0.9825026684948384 1104 32-33 0.7979471361105013 1104 34-35 0.8718835620544425 1104 36-37 0.737092830107315 1104 38-39 0.8030960879704026 1104 40-41 0.9089594439187962 1104 42-43 0.9995408906980288 1104 44-45 0.9515044558216204 1104 46-47 0.7705721977695319 1104 48-49 0.9371478785789691 1104 50-51 0.8337629373783031 1104 52-53 0.7917616835139185 1104 54-55 0.7959410227149348 1104 56-57 0.7949054588546822 1104 58-59 0.736911327006883 1104 60-61 0.7020957247738444 1104 62-63 0.7896785551860468 1104 64-65 0.7875763807176028 1104 66-67 -2.627659217875582 1104 68-69 -7.159017282596071 1104 70-71 -8.524399230529294 1104 72-73 -7.950720467162331 1104 74-75 -7.006738251276971 1104 76-77 -3.430660033295034 1104 78-79 -0.561307756376646 1104 80-81 0.26652970870556203 1104 82-83 0.386567441435421 1104 84-85 0.5251770869123185 1104 86-87 0.556074116128034 1104 88-89 0.7266130632428727 1104 90-91 0.6313675433465455 1104 92-93 0.48073995395040825 1104 94-95 1.3896503758710175 1104 96-97 1.398152216341071 1104 98-99 1.110120218853762 1104 100-101 0.9032865273062569 1105 1 0.48008898170472136 1105 2 0.36832190236368945 1105 3 0.4752060709850312 1105 4 0.3611716836530974 1105 5 0.4084945541008693 1105 6 0.518550848313275 1105 7 0.4721559168355398 1105 8 0.46126649948855913 1105 9 0.6630958034406405 1105 10-11 0.8070627989122059 1105 12-13 0.7295018047096704 1105 14-15 1.0327173816293964 1105 16-17 0.9778710114786477 1105 18-19 0.9287242699830003 1105 20-21 0.9356349053033952 1105 22-23 1.0380691639724517 1105 24-25 1.0802163141111194 1105 26-27 1.3038257878007897 1105 28-29 1.3481018618330936 1105 30-31 1.2339602163233465 1105 32-33 1.120337514549263 1105 34-35 1.2732136835458547 1105 36-37 1.2284487859794027 1105 38-39 1.217800738158921 1105 40-41 1.302289635232853 1105 42-43 1.33409088371576 1105 44-45 1.2341884385055977 1105 46-47 1.238740051421253 1105 48-49 0.8504595668906632 1105 50-51 0.954842395554536 1105 52-53 1.309768945069564 1105 54-55 1.2599666476276568 1105 56-57 1.2072361389272963 1105 58-59 1.3090154325787289 1105 60-61 1.348841989262045 1105 62-63 1.4112362985179807 1105 64-65 1.3207369029910296 1105 66-67 1.9654702333183849 1105 68-69 -4.435144918401125 1105 70-71 -5.0260557729116435 1105 72-73 -1.298765432949228 1105 74-75 1.1701923450084593 1105 76-77 1.1879180284281858 1105 78-79 1.120510425441534 1105 80-81 1.146174660527933 1105 82-83 1.2127247518669257 1105 84-85 1.0728886495432377 1105 86-87 1.3132693402587385 1105 88-89 1.4736037070077082 1105 90-91 1.4331166007407674 1105 92-93 1.6040188256163752 1105 94-95 2.479911861697019 1105 96-97 2.1896059239238106 1105 98-99 1.828174610698479 1105 100-101 1.7396484181036307 1106 1 0.04356389476979672 1106 2 0.08035085701559552 1106 3 0.08140683743169674 1106 4 -0.03681816802527749 1106 5 0.2910117636672567 1106 6 0.2743630688795946 1106 7 0.20388184118441188 1106 8 0.21707339750857102 1106 9 0.3897777280733834 1106 10-11 0.43949484773769854 1106 12-13 0.27080298986331286 1106 14-15 0.35917398513517895 1106 16-17 0.28337275017711505 1106 18-19 0.23574116019661062 1106 20-21 0.460686356584354 1106 22-23 0.5112594982283554 1106 24-25 0.5344415287871556 1106 26-27 0.3671738468455672 1106 28-29 0.4824145436867511 1106 30-31 0.49410587667392747 1106 32-33 0.7006485286717563 1106 34-35 0.5880831037431378 1106 36-37 0.45994229247290974 1106 38-39 0.4067493578452428 1106 40-41 0.5861099815532 1106 42-43 0.5598528949285964 1106 44-45 0.49863560336260093 1106 46-47 0.4970396752946513 1106 48-49 0.27403312448061 1106 50-51 0.11935697933141398 1106 52-53 0.29309695585659057 1106 54-55 0.40771609763120154 1106 56-57 0.2754889243931977 1106 58-59 0.5821960088330762 1106 60-61 0.5952474963232888 1106 62-63 0.40694455201312607 1106 64-65 0.30626578667564885 1106 66-67 1.0272632217507187 1106 68-69 -5.534951532552004 1106 70-71 -6.294974763756866 1106 72-73 -2.3785716922636873 1106 74-75 0.32013024863488937 1106 76-77 0.5613239258094964 1106 78-79 0.46287257149220906 1106 80-81 0.43754592587460905 1106 82-83 0.5968662251477461 1106 84-85 0.5736346931171497 1106 86-87 0.6376270863377975 1106 88-89 0.6378681311081991 1106 90-91 0.6949362019046283 1106 92-93 0.6203838813256937 1106 94-95 1.7616493182295585 1106 96-97 1.9711118496920328 1106 98-99 1.669771197172114 1106 100-101 1.4401673663684775 1107 1 -0.048637726369811674 1107 2 -0.12019682528547548 1107 3 -0.06542539899426814 1107 4 -0.07466964892736172 1107 5 -0.03216985395484073 1107 6 0.04617177810811768 1107 7 0.09906161982819839 1107 8 0.11374626609875804 1107 9 0.2582633170864099 1107 10-11 0.2237765199737467 1107 12-13 0.26665124946780594 1107 14-15 0.2082773289284745 1107 16-17 -0.006154021459430226 1107 18-19 0.22688444314317024 1107 20-21 0.33823230790079606 1107 22-23 0.1883373740232699 1107 24-25 0.1334465983196651 1107 26-27 0.25965347460673627 1107 28-29 0.3701703730320318 1107 30-31 0.0830920627378049 1107 32-33 0.37851321565975127 1107 34-35 0.4282343874795984 1107 36-37 0.31500534536204583 1107 38-39 0.3979955433537299 1107 40-41 0.37775312102242964 1107 42-43 0.4868647402939672 1107 44-45 0.30126447840773096 1107 46-47 0.3462593437231547 1107 48-49 0.44501537340298825 1107 50-51 0.490297882096975 1107 52-53 0.6512191615197835 1107 54-55 0.6531649348476876 1107 56-57 0.680462188240309 1107 58-59 0.6765220201940174 1107 60-61 0.5638335194709647 1107 62-63 0.5617397805432063 1107 64-65 0.39575337202923677 1107 66-67 1.320095680335136 1107 68-69 -4.74624505015343 1107 70-71 -5.982683384430562 1107 72-73 -2.119158807690429 1107 74-75 0.3592333764407982 1107 76-77 0.358760772640494 1107 78-79 0.46360754571256635 1107 80-81 0.409149909916227 1107 82-83 0.5472462086709946 1107 84-85 0.5594663834035778 1107 86-87 0.6528373809137378 1107 88-89 0.5457851487543692 1107 90-91 0.32757359302384614 1107 92-93 0.5997839300771304 1107 94-95 1.3895556599277725 1107 96-97 1.6536093118402455 1107 98-99 1.0680696793978974 1107 100-101 1.0467136354949318 1108 1 0.006764154780672271 1108 2 -0.1179162434075316 1108 3 0.00988653319467403 1108 4 -0.042594297447507756 1108 5 0.06689484382411592 1108 6 0.026909869318210156 1108 7 0.06590749599567403 1108 8 0.1490623288213513 1108 9 0.14266989342328884 1108 10-11 0.13194025608537885 1108 12-13 0.154613819802222 1108 14-15 0.15528126392090513 1108 16-17 0.005489469544578185 1108 18-19 0.09873243950478638 1108 20-21 0.0512041609504692 1108 22-23 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82-83 -6.803630887096663 2315 84-85 -5.220874281179949 2315 86-87 -5.538496778803747 2315 88-89 -3.9816830735980133 2315 90-91 -3.8394184370052606 2315 92-93 -5.133417928012722 2315 94-95 -3.0866325074111387 2315 96-97 -2.0335597720093013 2315 98-99 -3.649475948588492 2315 100-101 -3.0022673574848646 2316 1 -0.4821019963313269 2316 2 -0.40247303040307614 2316 3 -0.999192183342803 2316 4 -0.3279050846490179 2316 5 -0.2923597559083717 2316 6 -1.999136863036405 2316 7 -0.7022717681555264 2316 8 -0.55876921596969 2316 9 -0.09308404957020144 2316 10-11 -0.13928662631046507 2316 12-13 -0.7175259328110286 2316 14-15 -0.873511366148648 2316 16-17 -1.6792213093460617 2316 18-19 -0.32757518173898603 2316 20-21 -4.358704558050157 2316 22-23 -4.361958625139845 2316 24-25 -2.9286477678163436 2316 26-27 -2.220375070026833 2316 28-29 -1.35538361982141 2316 30-31 -0.46911751580368133 2316 32-33 -1.8815904283998037 2316 34-35 -2.1292353931990036 2316 36-37 -4.479079014858261 2316 38-39 -1.9442748622969575 2316 40-41 -1.547692835348208 2316 42-43 -2.9133122413282067 2316 44-45 -0.688230105391817 2316 46-47 -0.3688085033103974 2316 48-49 -0.2893237718363366 2316 50-51 -0.038864015405394525 2316 52-53 -2.7235469174716513 2316 54-55 -1.0149893236311307 2316 56-57 0.21806451243173441 2316 58-59 0.24821854167620572 2316 60-61 -1.2996820219380218 2316 62-63 -0.8286429536852111 2316 64-65 0.1780571062619387 2316 66-67 1.3486158435766704 2316 68-69 3.5164533659436366 2316 70-71 4.102232312242638 2316 72-73 2.2890493344430034 2316 74-75 1.360386540484349 2316 76-77 -0.2814251284149698 2316 78-79 0.19289051014556335 2316 80-81 -0.40265708284606205 2316 82-83 -0.14366269871066706 2316 84-85 0.22684707374814295 2316 86-87 -0.006757463922991036 2316 88-89 0.5920040859714675 2316 90-91 0.542339537428493 2316 92-93 0.8423087047513249 2316 94-95 1.332330276615437 2316 96-97 1.0123852572665264 2316 98-99 0.05344376642538151 2316 100-101 -0.07827925281699777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 30.0 4 59.0 5 124.0 6 359.0 7 893.0 8 1806.0 9 3008.0 10 4881.0 11 7097.0 12 9535.0 13 11841.0 14 14283.0 15 18103.0 16 21375.0 17 24198.0 18 21664.0 19 18501.0 20 17497.0 21 17475.0 22 18373.0 23 20860.0 24 25318.0 25 31332.0 26 39522.0 27 49289.0 28 59753.0 29 70929.0 30 83702.0 31 96397.0 32 111637.0 33 129779.0 34 149940.0 35 170469.0 36 178383.0 37 145960.0 38 58400.0 39 3385.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.978998488189475 20.360169141825924 15.442841885893458 22.217990484091143 2 16.330834715907077 21.403892072442382 38.51402155959209 23.751251652058457 3 19.522333363077742 25.392106649586044 30.441362163404357 24.64419782393186 4 13.491695810072596 17.72770666963296 35.87267031015266 32.907927210141786 5 13.457477595420011 38.07443155044033 33.5076438076693 14.960447046470366 6 31.538808504292366 36.444435208738504 16.877668592718813 15.139087694250325 7 27.94325989724734 33.81370548882079 20.080529923076078 18.162504690855798 8 26.8884132500327 34.63599570213707 19.091813646814924 19.383777401015305 9 26.611790275046115 16.698040903040162 20.25025639270439 36.43991242920933 10-11 25.23005056956463 27.10847092158356 26.266347708845455 21.395130800006356 12-13 26.27621836957465 24.72352982161913 27.28657064520506 21.71368116360116 14-15 23.096459886001508 25.72025264001975 26.34448789300815 24.838799580970587 16-17 21.69145240429397 29.009519874632982 27.031591119673188 22.26743660139987 18-19 21.472208529768686 26.723377362763163 30.447996367060163 21.35641774040799 20-21 22.535092043175446 25.705072512707332 30.682576261573203 21.077259182544022 22-23 22.1108301011758 25.928117142434555 30.46538178982277 21.49567096656688 24-25 21.838363193864666 26.235146641958433 30.583493566040527 21.34299659813637 26-27 21.644231465633023 26.351452321339675 30.539590803202504 21.464725409824794 28-29 21.348630218633183 26.517121120888593 30.698324107724144 21.43592455275408 30-31 21.570974044370068 26.20983502951108 30.611391065415177 21.607799860703675 32-33 21.3824835498669 26.325530789942576 30.463507140870593 21.82847851931993 34-35 22.01956860319269 26.31477125749089 30.331121010667562 21.334539128648867 36-37 22.015984171228737 26.107469476754858 30.192133090893076 21.684413261123332 38-39 21.660334738196504 26.421257658961792 30.309007694677355 21.60939990816435 40-41 21.825928735095523 26.35414241293209 30.315197327619625 21.504731524352763 42-43 21.780781310848234 26.44150569719211 29.939481004414038 21.838231987545623 44-45 21.786197274522888 26.611501282165406 29.823048020558694 21.779253422753015 46-47 22.00953615335177 26.88235328306566 29.128552694328874 21.97955786925369 48-49 22.111224535682396 27.030468926137154 28.876757184369104 21.981549353811346 50-51 22.018329820525608 27.274081597068207 28.70886726481268 21.99872131759351 52-53 22.437431512075744 27.249989830986404 28.458833233376264 21.853745423561588 54-55 21.97918870950183 27.2171779381239 28.65671523123724 22.146918121137023 56-57 22.13164745031083 27.488341024461306 28.313571796514005 22.066439728713867 58-59 22.206118281643658 27.11121489357085 28.3381232538287 22.34454357095679 60-61 22.078541660808877 27.093401092177373 28.715163465970008 22.11289378104374 62-63 22.10516375740355 27.124066658741437 28.737146836930812 22.033622746924213 64-65 21.889867013433125 27.17433724464302 28.599645185788486 22.33615055613537 66-67 21.485831726545968 27.273028623834968 29.005750058163553 22.23538959145551 68-69 21.670964608544192 26.80797075098522 29.487938849244554 22.033125791226034 70-71 21.196981634016645 26.873353129201043 29.722088721105923 22.20757651567639 72-73 21.916917358674677 27.156748089469463 28.483822090808125 22.442512461047734 74-75 21.587278947315262 27.518011870280866 28.396697918155606 22.49801126424827 76-77 21.770756095816594 27.46098976904085 28.22958010705922 22.53867402808333 78-79 21.789152397540253 27.473433248544254 28.122936112353113 22.614478241562377 80-81 21.70210789323137 27.65842815022712 28.024076238161133 22.61538771838038 82-83 21.845636312296705 27.690228718183164 27.86628096728808 22.597854002232054 84-85 21.69638458783421 27.617772567753068 27.778545156286484 22.90729768812624 86-87 21.7343100134156 27.536999856370574 27.88852523141145 22.840164898802374 88-89 21.670255884162245 27.5145532150007 27.713037400899243 23.10215349993781 90-91 21.568415924914262 27.59682611669314 27.870922551942023 22.96383540645057 92-93 21.647071550899017 27.49724653784987 27.912374505079697 22.943307406171424 94-95 21.114295489260456 27.281350682732104 28.28288304347733 23.32147078453011 96-97 21.094581340540977 27.140958905032708 28.273442865185643 23.49101688924067 98-99 21.27573670432647 27.173833643811502 27.742291405281744 23.80813824658028 100-101 21.16002435169123 27.095901898869577 28.008173464235895 23.73590028520329 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2636.0 1 1753.0 2 933.5 3 1019.0 4 966.0 5 960.5 6 1367.0 7 2425.5 8 3627.0 9 4259.0 10 4040.0 11 3390.5 12 3036.5 13 3021.0 14 3100.5 15 3155.5 16 3289.5 17 3511.0 18 3723.0 19 3975.5 20 4394.5 21 4890.5 22 5527.0 23 6343.0 24 7381.5 25 8807.0 26 10830.5 27 13102.0 28 15123.5 29 17195.5 30 19610.0 31 22862.5 32 26687.5 33 29794.0 34 32331.5 35 35015.0 36 38338.5 37 41697.0 38 43433.5 39 44037.5 40 45175.5 41 46993.5 42 48469.5 43 49929.5 44 51980.5 45 54358.5 46 55859.5 47 57293.5 48 60853.0 49 61740.5 50 59261.0 51 57148.0 52 55184.0 53 54197.0 54 53067.5 55 49877.0 56 45635.5 57 42007.0 58 38737.5 59 34470.5 60 29299.0 61 24269.5 62 19823.0 63 15403.5 64 11358.0 65 8528.0 66 6430.5 67 4873.5 68 3545.5 69 2487.0 70 1847.5 71 1392.0 72 1129.0 73 945.0 74 734.0 75 548.5 76 373.0 77 235.5 78 151.0 79 98.0 80 59.5 81 42.5 82 32.5 83 17.5 84 8.5 85 8.5 86 7.0 87 2.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0229194908572623 2 0.019802440100674626 3 0.0 4 0.0 5 6.11186422860328E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.6671185371619684E-4 18-19 0.0013140508091497051 20-21 3.05593211430164E-5 22-23 0.0 24-25 0.0 26-27 3.361525325731804E-4 28-29 0.019374609604672396 30-31 0.0049811693463116735 32-33 0.00959562683890715 34-35 0.010634643757769707 36-37 0.10206813261767478 38-39 0.10515462405311943 40-41 0.12000645412862541 42-43 0.1049101494839753 44-45 0.09916499710908823 46-47 0.10057072588166698 48-49 0.10350442071139654 50-51 0.10188477669081668 52-53 0.07905696379698343 54-55 0.06649708280720369 56-57 0.03786299889619732 58-59 0.015982524957797575 60-61 0.01002345733490938 62-63 0.00229194908572623 64-65 0.0057757116960301 66-67 0.43608151271084405 68-69 0.8361030264729288 70-71 0.9341984473420113 72-73 0.28591300861406144 74-75 0.15808336827282382 76-77 0.014974067360078036 78-79 0.008648287883473642 80-81 0.0015890846994368528 82-83 0.0 84-85 0.0 86-87 4.278304960022296E-4 88-89 7.028643862893772E-4 90-91 0.0036059998948759354 92-93 0.002444745691441312 94-95 0.2269640781291828 96-97 0.23353433217493136 98-99 0.01494350803893502 100-101 0.008312135350900461 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1636162.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.14022108115211 #Duplication Level Percentage of deduplicated Percentage of total 1 78.86988589021809 45.855126023025186 2 12.372336474403717 14.386607558244682 3 3.8499269662049307 6.715068148843317 4 1.665243606701575 3.8727052579041876 5 0.8234957018326154 2.393911108196339 6 0.5084584175314362 1.7737130883510268 7 0.34165403856962234 1.3904688934994418 8 0.22938881081555193 1.066937293948701 9 0.17616662540344882 0.921812989326932 >10 1.0358427036831255 11.137519857456969 >50 0.08513232636153255 3.3787830245543145 >100 0.0397081630621763 4.1618045367191145 >500 0.002017124193522094 0.7753181162284581 >1k 4.246577249520198E-4 0.569591240869056 >5k 2.123288624760099E-4 0.9649349662446849 >10k+ 1.0616443123800495E-4 0.6356978965876037 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10299 0.6294608969038518 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9460 0.5781823560258703 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6173 0.37728537883168045 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3384 0.20682548549593502 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2688 0.16428691046485616 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1945 0.11887575924633381 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.11186422860328E-5 0.0 12-13 0.0 0.0 0.0 6.11186422860328E-5 6.11186422860328E-5 14-15 0.0 0.0 0.0 1.222372845720656E-4 6.11186422860328E-5 16-17 0.0 0.0 0.0 1.222372845720656E-4 6.11186422860328E-5 18-19 0.0 0.0 0.0 1.222372845720656E-4 1.222372845720656E-4 20-21 0.0 0.0 0.0 1.222372845720656E-4 1.222372845720656E-4 22-23 0.0 0.0 0.0 2.750338902871476E-4 1.222372845720656E-4 24-25 0.0 0.0 0.0 7.028643862893772E-4 1.222372845720656E-4 26-27 0.0 0.0 0.0 0.0011612542034346233 1.222372845720656E-4 28-29 0.0 0.0 0.0 0.0025364236548703614 1.222372845720656E-4 30-31 0.0 0.0 0.0 0.00687584725717869 1.833559268580984E-4 32-33 0.0 0.0 0.0 0.016318677490370758 1.833559268580984E-4 34-35 0.0 0.0 0.0 0.030864914354446563 1.833559268580984E-4 36-37 0.0 0.0 0.0 0.055067896699715554 1.833559268580984E-4 38-39 0.0 0.0 0.0 0.08834699742446042 1.833559268580984E-4 40-41 0.0 0.0 0.0 0.1252320980440812 1.833559268580984E-4 42-43 0.0 0.0 0.0 0.1631867749037076 1.833559268580984E-4 44-45 0.0 0.0 0.0 0.21049260403309697 1.833559268580984E-4 46-47 0.0 0.0 0.0 0.2692276192699745 1.833559268580984E-4 48-49 0.0 0.0 0.0 0.3321187021823022 1.833559268580984E-4 50-51 0.0 0.0 0.0 0.4011827679655193 1.833559268580984E-4 52-53 0.0 0.0 0.0 0.467679850772723 1.833559268580984E-4 54-55 0.0 0.0 0.0 0.5425196282519702 1.833559268580984E-4 56-57 0.0 0.0 0.0 0.6328224222295836 1.833559268580984E-4 58-59 0.0 0.0 0.0 0.7207721484791848 1.833559268580984E-4 60-61 0.0 6.11186422860328E-5 0.0 0.8138558406808127 1.833559268580984E-4 62-63 0.0 1.222372845720656E-4 0.0 0.9042197533007124 2.444745691441312E-4 64-65 0.0 1.222372845720656E-4 0.0 0.9938196828920364 2.444745691441312E-4 66-67 0.0 1.222372845720656E-4 0.0 1.088461900471958 2.444745691441312E-4 68-69 0.0 1.222372845720656E-4 0.0 1.209293456271445 2.750338902871476E-4 70-71 0.0 1.222372845720656E-4 0.0 1.3708605871545727 3.05593211430164E-4 72-73 0.0 1.222372845720656E-4 0.0 1.5473712260766352 3.05593211430164E-4 74-75 0.0 1.222372845720656E-4 0.0 1.7365945425941929 3.05593211430164E-4 76-77 0.0 1.222372845720656E-4 0.0 1.9483400787941536 3.05593211430164E-4 78-79 0.0 1.222372845720656E-4 0.0 2.192967444544 3.05593211430164E-4 80-81 0.0 1.222372845720656E-4 0.0 2.464487012899701 3.05593211430164E-4 82-83 0.0 1.222372845720656E-4 0.0 2.747894157180035 3.05593211430164E-4 84-85 0.0 1.52796605715082E-4 0.0 3.027909216813494 3.05593211430164E-4 86-87 0.0 1.833559268580984E-4 0.0 3.322164920099599 3.667118537161968E-4 88-89 0.0 1.833559268580984E-4 0.0 3.642334927714982 3.667118537161968E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6120 0.0 44.914646 1 GTATCAA 9895 0.0 35.730114 1 TATAACG 100 5.042887E-5 28.426054 2 ATCAACG 12410 0.0 28.129898 3 TATCAAC 12510 0.0 28.06249 2 TCAACGC 12560 0.0 27.831661 4 CAACGCA 12875 0.0 27.077152 5 AACGCAG 13065 0.0 26.683378 6 GTACATG 9415 0.0 24.514328 1 TACATGG 9675 0.0 23.504747 2 ACGCAGA 14930 0.0 23.191563 7 ATAACGC 185 2.612942E-7 23.04322 3 GAGTACT 8755 0.0 23.020555 12-13 CGCAGAG 15095 0.0 22.93806 8 ACATGGG 9700 0.0 22.169533 3 GTACTTT 9320 0.0 21.574173 14-15 GCAGAGT 15870 0.0 21.280657 9 TAAGGTG 865 0.0 20.80846 5 GTGGTAT 3545 0.0 20.588081 1 AGTACTT 9035 0.0 20.472239 12-13 >>END_MODULE