FastQCFastQC Report
Fri 27 May 2016
SRR939528_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939528_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624019
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71270.43884954547945565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57740.35553771230509No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38400.23645043561682466No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21020.12943198324650143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54350.042.316071
GTATCAA90300.038.065241
ATCAACG118400.029.4086323
TATCAAC116150.029.0994952
TCAACGC122150.028.884874
CAACGCA122200.028.15595
GTACATG82350.027.3196371
AACGCAG125550.027.186736
TACATGG85350.026.1523822
ACATGGG88950.024.817313
TAAGGTG7950.024.3823285
ACGCAGA137900.024.1550947
CGCAGAG138100.024.119358
GTAAGGT6200.023.8397754
GAGTACT74100.022.38984312-13
GCAGAGT137350.022.3130849
CATGGGA52650.021.7342684
AGTACTT77100.020.89581912-13
GTACTTT81250.020.70608314-15
CATGGGG37500.019.8346944
Produced by FastQC (version 0.11.5)