FastQCFastQC Report
Fri 27 May 2016
SRR939528_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939528_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624019
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100630.6196356077114862No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90730.5586757297790236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62670.3858945000027709No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32680.20122917281140185No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25100.15455484203078906No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19290.11877939851688928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62100.044.7110751
GTATCAA98400.034.728681
TATCAAC127200.026.85732
ATCAACG127600.026.6946243
TCAACGC128800.026.4483624
CAACGCA131300.026.0299155
AACGCAG134800.025.3540636
GTACATG89150.024.9690691
TACATGG89600.024.3064542
ACATGGG90700.022.9618723
ACGCAGA153750.022.0749687
CGCAGAG155950.021.733168
GAGTACT89750.021.07109612-13
TAAGGTG7900.020.4011215
GCAGAGT160700.020.2648359
AGTACTT92150.020.13655312-13
GTACTTT94400.020.08337414-15
ATGGGTA7050.019.4989435
CATGGGA54050.018.8726524
AGAGTAC145350.018.83269310-11
Produced by FastQC (version 0.11.5)