FastQCFastQC Report
Fri 27 May 2016
SRR939527_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939527_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640205
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79030.48183001515054524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61820.37690410649888273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42590.25966266411820477No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22190.1352879670528989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA108750.035.709591
GGTATCA68350.034.818411
TATCAAC135600.028.5316242
ATCAACG138050.028.395123
TCAACGC139600.028.0307874
GTATCGA1255.1294483E-627.8809491
CAACGCA140150.027.7346325
GTACATG86350.027.5027141
AACGCAG144100.027.016326
TACATGG88150.026.2234822
ATCGACG1151.2175492E-424.9717883
ACATGGG92450.024.4878393
CGCAGAG158750.024.3926818
ACGCAGA159500.024.1748397
CATGGGA57700.022.8823554
GCAGAGT157800.022.8362189
GAGTACT80150.022.42346212-13
ATGGGAT23100.021.71215
GTACTTT88350.020.83659214-15
AGTACTT83750.020.7452212-13
Produced by FastQC (version 0.11.5)