FastQCFastQC Report
Fri 27 May 2016
SRR939527_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939527_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640205
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108790.6632707496928738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99260.6051682564069736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69430.4233007459433425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35180.2144853844488951No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26290.16028484244347507No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21050.12833761633454355No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20470.12480147298660837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79500.033.4364931
GTATCAA119800.030.652781
ATCAACG152150.024.0672553
TATCAAC153800.023.843492
TCAACGC154050.023.7396684
CAACGCA156100.023.3368635
CGCTCCG2850.023.29943348-49
AACGCAG159300.022.8978126
GTATAGG4800.022.7044811
CGATCGT2750.022.40311824-25
GTACATG92450.022.1924841
CCGCGAT2800.022.00138722-23
TACATGG94450.021.570082
CGCGATC2950.020.8826722-23
GAGTACT94950.020.85516412-13
AGTACTT98000.020.78617912-13
CAATTCG2550.020.43547678-79
AATTCGC2550.020.43547678-79
ATTCGCG2700.020.17683880-81
ACGCAGA180450.020.1615247
Produced by FastQC (version 0.11.5)