FastQCFastQC Report
Fri 27 May 2016
SRR939526_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939526_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608375
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78410.48751068625165156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59040.3670785730939613No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40510.25186912256159166No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22070.1372192430247921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA111900.034.34831
GGTATCA72050.033.688521
TATCAAC137750.027.8375262
ATCAACG139300.027.8199423
TCAACGC142000.027.4298974
CAACGCA142750.027.116935
AACGCAG145050.026.4303876
GTACATG83300.026.3750721
GTATCGA1358.958334E-625.9500621
TACATGG83200.025.3314552
ACGCAGA159800.023.5667087
CGCAGAG160700.023.2833718
GAGTACT78450.023.10425212-13
ACATGGG88050.023.0984733
TATCGAC1502.4682227E-522.7670362
GCAGAGT156450.022.2444529
GTACTTT86300.021.33730714-15
ATCGACG1604.5447057E-521.035383
CATGGGA54500.020.9070074
AGTACTT81600.020.682512-13
Produced by FastQC (version 0.11.5)