FastQCFastQC Report
Fri 27 May 2016
SRR939526_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939526_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608375
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104790.6515271625087433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94460.5873008471283127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67450.41936737390223056No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35620.22146576513561828No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24700.1535711510064506No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21130.1313748348488381No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19220.11949949483174012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78900.035.661531
GTATCAA113800.029.9367371
ATCAACG146200.023.0305083
TATCAAC147400.023.0081752
GTACATG95750.022.8446461
TCAACGC148800.022.6316154
CAACGCA151050.022.3127125
TACATGG98250.022.1591842
AACGCAG154050.021.9397286
TGTCGCG550.002687386721.84797564-65
ACATGGG99950.021.2563063
GAGTACT87700.020.8881212-13
AGTACTT90400.020.86719512-13
GTACTTT93400.019.46112614-15
ACGCAGA175150.019.2425657
AGAGTAC147450.019.23929210-11
CATGGGA64700.019.1335854
CGCAGAG177250.018.8808678
AGTACAT76350.018.3267972
GAGTACA85250.018.2558361
Produced by FastQC (version 0.11.5)