##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939526_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1608375 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.690774850392476 31.0 30.0 31.0 28.0 34.0 2 30.944848682676614 31.0 31.0 33.0 28.0 34.0 3 30.924511385715395 31.0 31.0 34.0 27.0 34.0 4 34.864936348799255 35.0 35.0 37.0 32.0 37.0 5 34.414847283749125 35.0 35.0 37.0 30.0 37.0 6 34.49809528250564 35.0 35.0 37.0 30.0 37.0 7 34.32566845418513 35.0 35.0 37.0 30.0 37.0 8 34.22666666666667 35.0 35.0 37.0 30.0 37.0 9 35.68612854589259 37.0 35.0 39.0 30.0 39.0 10-11 35.46652024558949 37.0 34.5 39.0 30.0 39.0 12-13 35.44119810367607 37.0 34.0 39.0 30.0 39.0 14-15 36.18203714929665 38.0 34.0 40.0 29.5 41.0 16-17 36.09007818450299 38.0 34.0 40.0 29.5 41.0 18-19 36.12102743452242 38.0 34.0 40.0 29.0 41.0 20-21 36.00488567653688 38.0 34.0 40.0 29.0 41.0 22-23 35.832903396285076 38.0 34.0 40.0 28.0 41.0 24-25 35.6420340405689 38.0 34.0 40.0 27.0 41.0 26-27 35.12192274811533 37.5 33.0 40.0 27.0 41.0 28-29 34.882935260744546 37.0 33.0 40.0 26.5 41.0 30-31 34.527872231289344 37.0 33.0 39.0 25.0 40.0 32-33 34.23115504779669 37.0 32.5 39.0 25.0 40.0 34-35 34.415875029144324 37.0 33.0 39.5 25.0 40.5 36-37 34.21116561747105 37.0 33.0 40.0 24.5 41.0 38-39 33.97974632781534 37.0 33.0 40.0 24.0 41.0 40-41 33.784168803917 37.0 32.0 40.0 24.0 41.0 42-43 33.54800683920106 37.0 32.0 39.0 23.5 40.5 44-45 33.0913337996425 36.0 31.0 39.0 21.5 40.0 46-47 32.59194186679102 36.0 31.0 39.0 19.0 40.0 48-49 32.1251561358514 36.0 30.0 39.0 15.5 40.0 50-51 31.82038144089531 35.5 30.0 39.0 14.0 40.0 52-53 31.66816351907982 35.0 30.0 39.0 13.5 40.0 54-55 31.297505245978083 35.0 29.5 39.0 10.5 40.0 56-57 30.748210771741665 35.0 28.5 38.0 8.0 40.0 58-59 30.250494754021915 34.0 28.0 38.0 7.5 40.0 60-61 29.84330115800109 34.0 27.0 38.0 6.5 40.0 62-63 29.64600606201912 34.0 27.0 38.0 2.0 40.0 64-65 29.1474597031165 34.0 26.0 37.0 2.0 39.5 66-67 28.198368850547915 32.5 24.5 36.5 2.0 39.0 68-69 24.73761560581332 30.0 12.0 35.0 2.0 38.0 70-71 23.38373451464988 28.0 10.0 34.0 2.0 37.5 72-73 24.371885287945908 29.0 14.5 34.0 2.0 37.0 74-75 25.089353540063726 30.0 19.0 34.0 2.0 37.0 76-77 24.855078883966737 29.5 19.0 34.0 2.0 36.0 78-79 25.984487137638922 31.0 22.5 34.0 2.0 36.0 80-81 25.988778114556617 31.0 22.5 34.0 2.0 36.0 82-83 25.632380819149763 31.0 21.5 34.0 2.0 36.0 84-85 25.24533084635113 31.0 20.0 34.0 2.0 35.0 86-87 24.760217921815496 31.0 19.0 34.0 2.0 35.0 88-89 24.222685940778735 30.5 17.5 34.0 2.0 35.0 90-91 23.574342115489237 30.0 9.0 34.0 2.0 35.0 92-93 22.939182404600917 29.5 4.5 34.0 2.0 35.0 94-95 22.011511307997203 29.0 2.0 34.0 2.0 35.0 96-97 21.261944509209606 29.0 2.0 33.5 2.0 35.0 98-99 20.347568819460637 28.5 2.0 33.0 2.0 34.5 100-101 18.944912411595553 26.0 2.0 32.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.30906620380346794 1101 2 0.17521850693511354 1101 3 0.3836304810774003 1101 4 0.4827871152608125 1101 5 0.6585596037223524 1101 6 0.36790514893036175 1101 7 0.5837236976340208 1101 8 0.6767796010482243 1101 9 0.7180032724047294 1101 10-11 0.8217665619073315 1101 12-13 0.8186401575543272 1101 14-15 0.7918116062058402 1101 16-17 0.8219842066950562 1101 18-19 0.5951952749871054 1101 20-21 0.8323109649733524 1101 22-23 0.7370134463744407 1101 24-25 0.8337395297598107 1101 26-27 0.8576956187450122 1101 28-29 0.8317636181788046 1101 30-31 0.47041734286712256 1101 32-33 0.9788394324653069 1101 34-35 0.8863480110230526 1101 36-37 1.1092971911457852 1101 38-39 1.1298520753828853 1101 40-41 1.093215320073675 1101 42-43 0.5196350223189725 1101 44-45 0.734031563155952 1101 46-47 0.6919617316977735 1101 48-49 1.0559699820690902 1101 50-51 0.7075993489643686 1101 52-53 0.3671727659993671 1101 54-55 0.3183784450115148 1101 56-57 0.4646852850608383 1101 58-59 0.32771886193818034 1101 60-61 0.3963270496349125 1101 62-63 -0.00656813537112555 1101 64-65 0.20450898578208765 1101 66-67 -0.024140273816342983 1101 68-69 -6.384991964645874 1101 70-71 -7.236364480994673 1101 72-73 -4.8291977712686105 1101 74-75 -2.831609974728252 1101 76-77 -0.12566043089412915 1101 78-79 0.13957587555227136 1101 80-81 0.1643628493690379 1101 82-83 -0.20216956502846628 1101 84-85 -0.31525407921759907 1101 86-87 -0.4181278254313945 1101 88-89 -0.6325125429309715 1101 90-91 -0.6712806438913397 1101 92-93 -0.5871337266284122 1101 94-95 -0.6489562135383125 1101 96-97 -1.089637608444054 1101 98-99 -1.0298049275487422 1101 100-101 -1.0149153594642968 1102 1 0.40557314513799625 1102 2 0.3272784614113782 1102 3 0.2892259343698065 1102 4 0.28718868734423353 1102 5 0.4306919516160903 1102 6 0.458012289880692 1102 7 0.44901299345440293 1102 8 0.5546560244821563 1102 9 0.6167467388552197 1102 10-11 0.6756043718865996 1102 12-13 0.5911655807845051 1102 14-15 0.7931044236325633 1102 16-17 0.6453228573052883 1102 18-19 0.45800707934182583 1102 20-21 0.6255465279672805 1102 22-23 0.6322549193908955 1102 24-25 0.44398749137953075 1102 26-27 0.675501522883664 1102 28-29 0.6049537010215502 1102 30-31 0.6663663514891738 1102 32-33 0.6212353996158697 1102 34-35 0.34831041308110144 1102 36-37 0.6064873698738467 1102 38-39 0.604819468925669 1102 40-41 0.43728944378535317 1102 42-43 0.3890857955578113 1102 44-45 0.5086190655939404 1102 46-47 0.41033428539627437 1102 48-49 0.37446996518020015 1102 50-51 0.15252685203433813 1102 52-53 0.04143779956467242 1102 54-55 -0.20567896414531717 1102 56-57 -0.15854097269851763 1102 58-59 -0.05013958299803889 1102 60-61 -0.1575131029970862 1102 62-63 -0.4023424256835497 1102 64-65 -0.3317430204548195 1102 66-67 -0.560492796199572 1102 68-69 -7.209339045537131 1102 70-71 -7.681967267334937 1102 72-73 -2.3952390586162124 1102 74-75 -0.771072286893812 1102 76-77 -0.024631624982365707 1102 78-79 -0.15803391041643877 1102 80-81 -0.4208454233549652 1102 82-83 -0.6378316041387784 1102 84-85 -0.9026115996231923 1102 86-87 -0.8475515982354871 1102 88-89 -0.9814027248188886 1102 90-91 -1.1013674418991037 1102 92-93 -0.9394221169862753 1102 94-95 -0.774053052264648 1102 96-97 -1.0205604065956457 1102 98-99 -1.0835148722730352 1102 100-101 -0.9910889467629822 1103 1 0.3518806196144837 1103 2 0.23246328758931512 1103 3 0.4322476222151792 1103 4 0.41451985466717645 1103 5 0.4775279826713046 1103 6 0.5268092293983102 1103 7 0.45459936379866406 1103 8 0.4418162390814615 1103 9 0.6465856805888919 1103 10-11 0.510275007215192 1103 12-13 0.5927157033637229 1103 14-15 0.7142736395226592 1103 16-17 0.700438900958396 1103 18-19 0.7303177478949152 1103 20-21 0.7038260183728156 1103 22-23 0.7435626685623191 1103 24-25 0.5741368655918251 1103 26-27 0.7814899813446203 1103 28-29 0.6961918571820789 1103 30-31 0.6022627517887855 1103 32-33 0.7554234693698945 1103 34-35 0.9183161463180483 1103 36-37 0.9430650952148056 1103 38-39 1.1621893709547635 1103 40-41 0.9931634822850768 1103 42-43 0.8730935185652271 1103 44-45 1.0138276624032372 1103 46-47 0.9405292426499372 1103 48-49 1.1326065108668608 1103 50-51 1.0671602322017968 1103 52-53 0.782759751280377 1103 54-55 0.6845200292201206 1103 56-57 0.7102455501694287 1103 58-59 0.7978287351563615 1103 60-61 0.8224099606619717 1103 62-63 0.6157212143344424 1103 64-65 0.721841530919388 1103 66-67 0.5726030212028164 1103 68-69 -6.010421425189467 1103 70-71 -6.871797244766437 1103 72-73 -0.7837493580445773 1103 74-75 0.6769755568053135 1103 76-77 1.128163219238406 1103 78-79 1.069551526508981 1103 80-81 0.4816178831126585 1103 82-83 0.5335112679083203 1103 84-85 0.4564392994451829 1103 86-87 0.4254553267967296 1103 88-89 0.5557354869877713 1103 90-91 0.4614502591163969 1103 92-93 0.44682983647550856 1103 94-95 0.5132363777460718 1103 96-97 0.4998864159998355 1103 98-99 0.5016182568613274 1103 100-101 0.6554155193415703 1104 1 0.24757121963995843 1104 2 0.322803381258872 1104 3 0.26646889362568515 1104 4 0.16793916669162456 1104 5 0.45576640114366285 1104 6 0.3990589132047333 1104 7 0.363580234797368 1104 8 0.5077159284451085 1104 9 0.5798292871507869 1104 10-11 0.546418427265472 1104 12-13 0.39303834655539305 1104 14-15 0.6802504809293737 1104 16-17 0.6314648933275606 1104 18-19 0.59993809656374 1104 20-21 0.5484705226891933 1104 22-23 0.7034594259860754 1104 24-25 0.7107268101900033 1104 26-27 0.7789340663952231 1104 28-29 0.5222999949783897 1104 30-31 0.4811681176282008 1104 32-33 0.8466766505733503 1104 34-35 0.486355567685834 1104 36-37 0.8019697471341587 1104 38-39 1.0818753921565616 1104 40-41 1.0465606465621349 1104 42-43 0.9983284834492849 1104 44-45 0.919021511981903 1104 46-47 0.8163886847700823 1104 48-49 0.9217352119947755 1104 50-51 0.766640855356659 1104 52-53 0.6626114668383956 1104 54-55 0.7623738232590505 1104 56-57 0.7499082716164835 1104 58-59 0.8062067493390295 1104 60-61 0.8994399071747559 1104 62-63 0.6936098931036767 1104 64-65 0.5881961009407526 1104 66-67 0.5462508525489369 1104 68-69 -6.0357132602966495 1104 70-71 -3.7615708266413073 1104 72-73 0.6897877345646464 1104 74-75 1.3790133118187775 1104 76-77 1.606532252631208 1104 78-79 1.1288535398446804 1104 80-81 0.8527451710033596 1104 82-83 0.7947204213370398 1104 84-85 0.585047130986009 1104 86-87 0.6079190973817639 1104 88-89 0.6810475227876189 1104 90-91 0.6637249057868111 1104 92-93 0.7120061138853764 1104 94-95 0.848511898157291 1104 96-97 0.7136738349435419 1104 98-99 0.9301179457382105 1104 100-101 1.181622470164644 1105 1 0.1825488290285726 1105 2 0.32147731660184675 1105 3 0.27143756882946235 1105 4 0.27578773777354115 1105 5 0.47527573791830946 1105 6 0.3232154048275575 1105 7 0.27451551159164467 1105 8 0.5456522971953106 1105 9 0.42241032903115894 1105 10-11 0.5439113405818219 1105 12-13 0.5102720857874985 1105 14-15 0.7250154346591202 1105 16-17 0.4889310040893946 1105 18-19 0.5618597183940253 1105 20-21 0.5634974631169314 1105 22-23 0.5284380556365349 1105 24-25 0.453222232799007 1105 26-27 0.6448287808873516 1105 28-29 0.6099189795696773 1105 30-31 0.37583931756643096 1105 32-33 0.7813758939251016 1105 34-35 0.795458095729245 1105 36-37 0.8391155508247508 1105 38-39 0.6833662153148552 1105 40-41 0.95920444833353 1105 42-43 0.8811212158759645 1105 44-45 1.0298560034503055 1105 46-47 1.017026762045269 1105 48-49 1.0458608200156867 1105 50-51 0.8732094114379727 1105 52-53 0.8211393392891218 1105 54-55 0.9498720309071551 1105 56-57 1.0258329873218983 1105 58-59 1.1025732384249878 1105 60-61 1.207101522166539 1105 62-63 0.9861490215243123 1105 64-65 1.0237996547609427 1105 66-67 1.059984817893124 1105 68-69 -2.845055549874534 1105 70-71 -2.979230318385458 1105 72-73 1.067302817895225 1105 74-75 1.528658150396808 1105 76-77 1.5195137059252737 1105 78-79 1.0694600993011285 1105 80-81 0.8293365037410787 1105 82-83 0.8117124688095494 1105 84-85 0.7856446735336853 1105 86-87 0.938066732028755 1105 88-89 0.815868646068342 1105 90-91 0.8345465198962039 1105 92-93 0.6544128840121068 1105 94-95 0.9370564256933314 1105 96-97 0.9299551459524444 1105 98-99 1.147393598430046 1105 100-101 1.1428282542220884 1106 1 0.06638501086789361 1106 2 0.014742634588127856 1106 3 0.11938257522406559 1106 4 0.044707049481480965 1106 5 0.13181276849615386 1106 6 0.11362349690987372 1106 7 0.1208153604464286 1106 8 0.22153010210171686 1106 9 0.23352822229938397 1106 10-11 0.3554390699156258 1106 12-13 0.19089207183563417 1106 14-15 0.26117111423126005 1106 16-17 0.1176485950671875 1106 18-19 0.29200737564318757 1106 20-21 0.3643098686511692 1106 22-23 0.36896095994999456 1106 24-25 0.4626644488203979 1106 26-27 0.628140314429956 1106 28-29 0.40058866914066016 1106 30-31 0.3119833705835191 1106 32-33 0.3775144243285453 1106 34-35 0.5396695246328704 1106 36-37 0.6689551043650326 1106 38-39 0.6515806671227864 1106 40-41 0.6256647436480307 1106 42-43 0.5293408996336666 1106 44-45 0.6677280532119596 1106 46-47 0.9020110661959464 1106 48-49 0.6616845614411027 1106 50-51 0.7448725901381188 1106 52-53 0.6507536283252797 1106 54-55 0.33203108218026145 1106 56-57 0.6640334872637652 1106 58-59 0.9120808538185479 1106 60-61 0.9206842451057327 1106 62-63 0.5704793319533294 1106 64-65 0.7294588804323752 1106 66-67 0.5205083035343279 1106 68-69 -3.162286104461213 1106 70-71 -3.099212006561249 1106 72-73 0.7366552187788393 1106 74-75 1.1061840194817947 1106 76-77 1.106449818005263 1106 78-79 0.8554922467258486 1106 80-81 0.6750288650944078 1106 82-83 0.5161886925044676 1106 84-85 0.40337226334882104 1106 86-87 0.6642933335867092 1106 88-89 0.6224594494632925 1106 90-91 0.3161707496369317 1106 92-93 0.36612954486549754 1106 94-95 0.5994866663630205 1106 96-97 0.3432631915285391 1106 98-99 0.36409078480372514 1106 100-101 0.5631282193424205 1107 1 0.07362125175265533 1107 2 0.18239216180795736 1107 3 0.24293019826813733 1107 4 -0.08310958268248925 1107 5 0.36046865540190964 1107 6 0.2807638762530189 1107 7 0.22401385351791703 1107 8 0.22290461486771562 1107 9 0.4319914536166394 1107 10-11 0.30454479736298623 1107 12-13 0.2896602323757449 1107 14-15 0.15858900714235347 1107 16-17 0.17472724256154493 1107 18-19 0.2911289556801151 1107 20-21 0.3316815093009424 1107 22-23 0.26582307972096686 1107 24-25 0.30998069865721334 1107 26-27 0.5374355269479096 1107 28-29 0.31318308529910865 1107 30-31 0.3874379669019916 1107 32-33 0.5570515287131812 1107 34-35 0.48833231207761685 1107 36-37 0.606839995914676 1107 38-39 0.7359322962094907 1107 40-41 0.4592946136567946 1107 42-43 0.5152796526876173 1107 44-45 0.6366513826287701 1107 46-47 0.6560096487879363 1107 48-49 0.6342204162686862 1107 50-51 0.841577116518696 1107 52-53 0.47219378946219237 1107 54-55 0.4284707770645362 1107 56-57 0.8037470216452292 1107 58-59 0.8926036162721509 1107 60-61 0.9181422357433782 1107 62-63 0.6403081239860064 1107 64-65 0.46459008392367807 1107 66-67 0.6887387815365287 1107 68-69 -2.273040687724606 1107 70-71 -2.907593363915918 1107 72-73 1.1292893596617368 1107 74-75 1.660517371465236 1107 76-77 1.1890968274608689 1107 78-79 1.020865528584892 1107 80-81 0.7612054069918734 1107 82-83 0.8381557191181699 1107 84-85 0.8775884926705189 1107 86-87 1.0404939341201036 1107 88-89 0.9145200996059657 1107 90-91 0.9850633063798782 1107 92-93 0.9375398658338092 1107 94-95 1.3732245204818945 1107 96-97 1.180776013475981 1107 98-99 1.3525109343775696 1107 100-101 1.573103228201223 1108 1 0.03248458515761854 1108 2 0.09082872753122118 1108 3 0.0760092357600044 1108 4 0.1607135680722891 1108 5 0.029535015220432115 1108 6 -0.06910943167027028 1108 7 0.06621465541266502 1108 8 0.23143247963572833 1108 9 0.2380483833248377 1108 10-11 0.20329581731397184 1108 12-13 0.1753905563571081 1108 14-15 0.1441159595711312 1108 16-17 0.36508526245327744 1108 18-19 0.3440078385757417 1108 20-21 0.3107582688751265 1108 22-23 0.29823049617289854 1108 24-25 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2.4322499475059693 2311 80-81 2.1832024627674436 2311 82-83 1.920154396497555 2311 84-85 1.911514218148195 2311 86-87 2.066918475066867 2311 88-89 2.1390476174626123 2311 90-91 2.252526585921572 2311 92-93 2.431841448671161 2311 94-95 2.87295594234973 2311 96-97 2.8667708040737203 2311 98-99 3.081532701875055 2311 100-101 3.1256995679373567 2312 1 -0.23505010963694062 2312 2 -0.06996920910806637 2312 3 -0.15856467694100473 2312 4 -0.03730801522497984 2312 5 -1.5424080409647942 2312 6 -0.2247825323906838 2312 7 -0.10290492991018851 2312 8 0.18752385576001274 2312 9 -2.8151535090737454 2312 10-11 -0.13624436617045887 2312 12-13 -1.426549192471775 2312 14-15 -4.284920436117588 2312 16-17 -1.066472153297525 2312 18-19 -0.11353828203849048 2312 20-21 0.009424092964046338 2312 22-23 0.3370042143709213 2312 24-25 0.4261359536247795 2312 26-27 0.45692454220353795 2312 28-29 0.4074286696533562 2312 30-31 -0.005672585017038045 2312 32-33 0.46910130273850115 2312 34-35 0.4780469284276663 2312 36-37 0.4991849201791254 2312 38-39 0.7897352619437044 2312 40-41 0.3998616273131219 2312 42-43 0.04393520785773575 2312 44-45 0.7336686146991198 2312 46-47 1.0719491581991818 2312 48-49 1.2425054725866822 2312 50-51 1.4266075948591954 2312 52-53 0.9428935771411666 2312 54-55 1.2605677206913946 2312 56-57 0.9363342516074908 2312 58-59 0.9864631624014955 2312 60-61 0.7936173340541508 2312 62-63 1.0177441007916919 2312 64-65 1.1671567489003145 2312 66-67 1.464879973683054 2312 68-69 3.9702739129704376 2312 70-71 4.991450524360705 2312 72-73 3.027949433435957 2312 74-75 2.5119853841066515 2312 76-77 2.335048407237803 2312 78-79 1.602903988310164 2312 80-81 1.715322896035591 2312 82-83 1.6848256057350035 2312 84-85 1.518081500842257 2312 86-87 1.5874616216532935 2312 88-89 1.8324638501007442 2312 90-91 1.8852310640048024 2312 92-93 2.130047522110356 2312 94-95 2.1985208759786268 2312 96-97 1.9814733283755466 2312 98-99 2.5270662565905084 2312 100-101 2.394407779449576 2313 1 -0.295236485201837 2313 2 -0.143949801934653 2313 3 -0.028763535459177092 2313 4 0.0635870142740913 2313 5 0.09419342398935981 2313 6 0.07912636362299708 2313 7 -3.271990350975191 2313 8 -3.379797510030162 2313 9 -1.0550489344590943 2313 10-11 -0.0796515768979873 2313 12-13 0.01965516764131081 2313 14-15 -0.6319065987551298 2313 16-17 -0.28021634545921614 2313 18-19 0.22853749141194868 2313 20-21 -2.5279591745145638 2313 22-23 -0.63209770681447 2313 24-25 -0.21567383555264996 2313 26-27 -2.040167530217417 2313 28-29 -1.7989645148057463 2313 30-31 -0.9675401071245773 2313 32-33 -1.0602473389750813 2313 34-35 -1.4749769955808247 2313 36-37 -2.1961590140442766 2313 38-39 -1.9131783211536444 2313 40-41 -1.0084399738982128 2313 42-43 -0.39331652509219595 2313 44-45 -0.21302462707312486 2313 46-47 -0.23700801670035077 2313 48-49 -2.022877974469214 2313 50-51 -0.2980969334618315 2313 52-53 -0.13873916516135054 2313 54-55 -0.1862021912516063 2313 56-57 -0.4664514701328457 2313 58-59 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-0.8884941178692678 2314 82-83 0.21670621098727239 2314 84-85 0.40348155296631205 2314 86-87 0.10287899736041695 2314 88-89 0.2458217124012556 2314 90-91 0.8025899074639966 2314 92-93 0.2100929006996317 2314 94-95 0.5693645191434769 2314 96-97 0.9813120504163528 2314 98-99 0.7158844202907062 2314 100-101 0.5045554131707739 2315 1 -0.3555392865235234 2315 2 -0.49356410372597637 2315 3 -0.5472431760283989 2315 4 -0.3657272903710407 2315 5 -0.33817259287788204 2315 6 -0.2476841597279673 2315 7 -1.2717062110691657 2315 8 -3.57656687652225 2315 9 -0.9048040654178067 2315 10-11 -1.704143320090104 2315 12-13 -0.8117837962530743 2315 14-15 -0.5961859318576828 2315 16-17 -0.3711178506562405 2315 18-19 -0.031594453730441785 2315 20-21 -0.7719270952432495 2315 22-23 -0.5554323553805602 2315 24-25 -5.529900311187966 2315 26-27 -0.9835746778870345 2315 28-29 -1.0602389133038983 2315 30-31 -1.1387967499805711 2315 32-33 -0.13434922732671595 2315 34-35 -2.3189863585490826 2315 36-37 -3.6995345713121175 2315 38-39 -1.146161256629071 2315 40-41 -0.2576849558509835 2315 42-43 -1.710374783699212 2315 44-45 -5.542400784881195 2315 46-47 -3.907259002814719 2315 48-49 -2.6005759697318958 2315 50-51 -1.1218759073569444 2315 52-53 -0.3298151868552459 2315 54-55 -1.1439379312127187 2315 56-57 -0.9833702023973672 2315 58-59 -0.7143905099376688 2315 60-61 -1.467447102466167 2315 62-63 -0.3086265298616766 2315 64-65 -0.06955096654397508 2315 66-67 0.41462557620373985 2315 68-69 2.863253297082661 2315 70-71 3.3448116152712153 2315 72-73 -2.0748220095200764 2315 74-75 -1.7220498893950307 2315 76-77 -0.5492365153371601 2315 78-79 -0.6398156644967941 2315 80-81 -0.5885946039871754 2315 82-83 -1.009771228574241 2315 84-85 -0.5376277093742345 2315 86-87 -0.1660531745099938 2315 88-89 -0.32510517416035967 2315 90-91 -0.14561243503706223 2315 92-93 -1.3274285504765153 2315 94-95 -0.4122165059454268 2315 96-97 -0.3739412855270068 2315 98-99 -2.9538565343274144 2315 100-101 -4.401714529082334 2316 1 -0.5577473181824679 2316 2 -0.47866892281438567 2316 3 -1.1368445393976216 2316 4 -0.647292807922561 2316 5 -0.254259171165387 2316 6 -0.7379295258698306 2316 7 -1.1025566265773392 2316 8 -1.2479711685301353 2316 9 -5.2396769783789665 2316 10-11 -1.5128870572290438 2316 12-13 -0.8212395340495036 2316 14-15 -0.49182391018829463 2316 16-17 -0.5950778396773373 2316 18-19 -0.3718893645113255 2316 20-21 -3.8919681730988884 2316 22-23 -1.3458186019748481 2316 24-25 -0.7825585865383076 2316 26-27 -0.6628076431375547 2316 28-29 0.09143630026528626 2316 30-31 -0.0542091645179994 2316 32-33 -1.336772417629298 2316 34-35 -0.6923819728793461 2316 36-37 -0.47601304071617534 2316 38-39 -5.760048797276607 2316 40-41 -5.236150095498637 2316 42-43 -3.875434027922495 2316 44-45 -2.641685062560679 2316 46-47 -2.989242199506158 2316 48-49 -3.3749656557630985 2316 50-51 -1.4713311489316432 2316 52-53 -1.3420819432553444 2316 54-55 -0.04193955323752263 2316 56-57 -3.6283630924702948 2316 58-59 -1.2051258950445067 2316 60-61 -1.0050680758981443 2316 62-63 -0.8336954193949637 2316 64-65 -0.5360866255031809 2316 66-67 -0.422322939240388 2316 68-69 1.8504848915206367 2316 70-71 3.043161910600684 2316 72-73 2.0812416650328984 2316 74-75 0.8202793754369608 2316 76-77 -2.018554892754107 2316 78-79 -0.36382721405771434 2316 80-81 -0.3740384045494558 2316 82-83 -0.10165133519988956 2316 84-85 0.2567403289837067 2316 86-87 0.38391402833459765 2316 88-89 -0.06817861652807977 2316 90-91 0.12435041036040317 2316 92-93 0.07364460656371108 2316 94-95 0.1850266721687106 2316 96-97 0.4268694942699476 2316 98-99 0.5941889723072258 2316 100-101 0.5554692885065542 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 24.0 4 86.0 5 222.0 6 521.0 7 1206.0 8 2418.0 9 3976.0 10 6228.0 11 8700.0 12 11434.0 13 14294.0 14 17721.0 15 21581.0 16 25133.0 17 27556.0 18 25053.0 19 22224.0 20 22117.0 21 22288.0 22 23493.0 23 25774.0 24 30302.0 25 37349.0 26 45476.0 27 56170.0 28 67469.0 29 78979.0 30 90883.0 31 103446.0 32 116924.0 33 131488.0 34 145711.0 35 152017.0 36 140532.0 37 97185.0 38 31247.0 39 1141.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.24359839427361 21.006843926753504 15.767586341997333 21.98197133697555 2 16.599404026057694 22.149660100837995 37.84571339026095 23.405222482843364 3 19.799897038744767 25.706926009654484 30.605543991066742 23.887632960534006 4 13.54378223124863 18.05348147263206 36.07130945980926 32.33142683631006 5 13.291427683220641 38.447563534623455 33.46508121551255 14.795927566643352 6 31.317385559959586 36.90924069324629 16.706427294629673 15.066946452164451 7 27.60923292142691 34.05138726975985 20.190875884044456 18.14850392476879 8 26.772922981269915 34.862329991451 19.046801896323927 19.317945130955156 9 26.541757985544418 16.692500194295484 20.365897256547758 36.39984456361234 10-11 25.29954915671693 27.315146014960124 26.12851505261911 21.256789775703837 12-13 26.32946296728064 24.808828786818996 27.313530737545662 21.548177508354705 14-15 23.0174244190565 25.81649180073055 26.524442371959278 24.641641408253673 16-17 21.53088479970952 29.095310514286567 27.17516059797409 22.198644088029823 18-19 21.318582180795918 26.86444021172969 30.600703879669588 21.2162737278048 20-21 22.48409108572317 25.794544182793196 30.76832206419523 20.953042667288415 22-23 21.978687792764617 25.970763019049052 30.694757562606707 21.355791625579624 24-25 21.765172937791142 26.172089703318484 30.903163790380546 21.159573568509828 26-27 21.522089889107544 26.323077257875475 30.874828624989508 21.28000422802747 28-29 21.283327867094226 26.579009952700194 30.934447098981416 21.20321508122417 30-31 21.43780214956557 26.30555192006324 30.81819094655681 21.438454983814374 32-33 21.340176315918733 26.382694702626207 30.66002513114266 21.6171038503124 34-35 21.89591382017725 26.369674033916933 30.559969259686326 21.17444288621949 36-37 21.929088756041452 26.088083369061927 30.57871604824573 21.404111826650894 38-39 21.56473418767674 26.5579880610985 30.537082536742744 21.340195214482016 40-41 21.697396470469645 26.42197935942795 30.58024075274745 21.300383417354958 42-43 21.54973428441912 26.4584955716144 30.205332605717654 21.786437538248826 44-45 21.694039258731042 26.647240153271586 29.981730863764263 21.676989724233113 46-47 22.000041689961712 26.940239928840835 29.308540873118467 21.751177508078985 48-49 21.984505886357002 27.04970971091799 29.116421134762454 21.849363267962552 50-51 21.887379906930786 27.34569195571653 28.924813541141038 21.84211459621165 52-53 22.22180057991715 27.37701022289049 28.579240309778527 21.821948887413832 54-55 21.833446700275534 27.322366131369424 28.75007425612469 22.094112912230347 56-57 22.09510702004078 27.557633291903677 28.407070757640255 21.94018893041528 58-59 22.182574157186682 27.19066787642324 28.492814531008925 22.133943435381152 60-61 22.052799144369065 27.30777840651175 28.9141073393982 21.725315109720988 62-63 21.90132950496256 27.35162835290731 28.884696858816806 21.862345283313324 64-65 21.740414556741648 27.37434961250336 28.75831948824577 22.12691634250923 66-67 21.754339857183606 27.42925506539835 28.61982763882125 22.196577438596798 68-69 22.336545853117904 26.670189725157584 29.340206534344183 21.653057887380328 70-71 22.05664082479765 26.56938676761585 29.7047713424488 21.669201065137702 72-73 22.076700255779855 27.022391112578397 28.805517131196623 22.09539150044513 74-75 21.750317026689338 27.71408904865244 28.227334807866427 22.308259116791792 76-77 21.2327894428105 27.66259161544426 28.73040394439787 22.37421499734737 78-79 21.740567410462223 27.630448192579287 28.252126048100052 22.376858348858438 80-81 21.68006001280215 27.85646046955106 28.084430775276243 22.37904874237055 82-83 21.820435969758986 27.769779565398107 28.010026452649633 22.399758012193274 84-85 21.64302989983648 27.76469344740327 27.902628126612655 22.68964852614759 86-87 21.71354511223967 27.76417855881578 27.869970603303774 22.652305725640783 88-89 21.591758343561104 27.786399364323795 27.737716415293534 22.88412587682157 90-91 21.538673897458633 27.827896042235718 27.830694015508232 22.802736044797417 92-93 21.68933333084625 27.660822640425444 27.93297183563606 22.716872193092247 94-95 21.45779987492572 27.41631379332875 28.041996334887948 23.083889996857586 96-97 21.373008856024985 27.36961721334851 28.031338952484347 23.22603497814216 98-99 21.377597324162657 27.388095595644614 27.68849925334363 23.545807826849096 100-101 21.21053461226472 27.245858712924758 28.05992293703602 23.483683737774502 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2658.0 1 1839.0 2 1026.0 3 1078.0 4 1053.5 5 1024.5 6 1506.0 7 2744.5 8 3900.5 9 4487.0 10 4279.5 11 3524.0 12 3235.0 13 3223.5 14 3281.0 15 3430.0 16 3524.0 17 3709.5 18 3916.5 19 4171.5 20 4645.0 21 5223.5 22 5863.5 23 6650.5 24 7697.0 25 9172.0 26 11005.5 27 13163.5 28 15163.5 29 17138.5 30 19425.5 31 22348.0 32 26154.5 33 29502.0 34 31917.5 35 34593.0 36 38240.0 37 41358.5 38 42881.5 39 43506.0 40 43923.5 41 45397.5 42 47205.5 43 48963.0 44 50687.0 45 52283.5 46 53729.5 47 56019.5 48 60471.0 49 61543.5 50 58642.5 51 56081.0 52 54097.0 53 53259.5 54 52172.5 55 49025.0 56 44826.0 57 40738.0 58 37318.5 59 33400.5 60 28567.5 61 23619.0 62 18846.0 63 14301.5 64 10654.5 65 8156.5 66 6080.5 67 4482.5 68 3156.5 69 2159.5 70 1585.0 71 1233.0 72 973.0 73 818.0 74 679.0 75 503.5 76 336.0 77 201.0 78 120.0 79 68.0 80 41.0 81 29.0 82 22.5 83 15.0 84 7.0 85 2.0 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023626330924069325 2 0.015605813320898422 3 1.86523665190021E-4 4 0.07703427372347867 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.010352063418046164 12-13 0.0 14-15 0.0 16-17 6.839201056967436E-4 18-19 2.1761094272169113E-4 20-21 0.0 22-23 6.217455506334032E-5 24-25 0.003170902308230357 26-27 0.00373047330380042 28-29 0.0010880547136084557 30-31 2.1761094272169113E-4 32-33 7.46094660760084E-4 34-35 0.003948084246522111 36-37 0.08405999844563612 38-39 0.09494054558172069 40-41 0.07765601927411207 42-43 0.08079583430481076 44-45 0.08039169969689905 46-47 0.0791792958731639 48-49 0.07361467319499494 50-51 0.07361467319499494 52-53 0.056205797777259654 54-55 0.04747027279086034 56-57 0.02664179684464133 58-59 0.02101499961140903 60-61 0.012745783787984768 62-63 0.0019895857620268903 64-65 0.006403979171524054 66-67 0.16644128390456206 68-69 0.7600217610942722 70-71 0.9305043910779514 72-73 0.3743219087588404 74-75 0.05350120463200435 76-77 0.20918629051060855 78-79 0.014113623999378254 80-81 0.003201989585762027 82-83 0.0018341493743685398 84-85 3.1087277531670164E-4 86-87 0.0019274112069635502 88-89 5.595709955700629E-4 90-91 0.004227869744307142 92-93 0.004103520634180461 94-95 0.08269215823424263 96-97 0.037273645760472526 98-99 0.012776871065516438 100-101 0.0130255692857698 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1608375.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.323455537234665 #Duplication Level Percentage of deduplicated Percentage of total 1 77.34608688669786 43.56398885742016 2 12.76175244145375 14.375719924268324 3 4.282324584081377 7.235859549225432 4 1.9134903096278812 4.310975455010215 5 1.0036016526398983 2.8263156529779274 6 0.6135673302901754 2.073493934801908 7 0.3887420543763522 1.5326707070583812 8 0.2814168005365986 1.2680293321963159 9 0.1942440252668384 0.9846445248441233 >10 1.0876585756588226 11.274804183765621 >50 0.08443860062447439 3.266018640605056 >100 0.03988388137454336 4.158526285655573 >500 0.0016757144228921339 0.601087667955441 >1k 7.820000640163292E-4 0.849752847873781 >5k 2.2342858971895118E-4 1.0187595971806285 >10k+ 1.1171429485947559E-4 0.6593528391610035 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10479 0.6515271625087433 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9446 0.5873008471283127 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6745 0.41936737390223056 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3562 0.22146576513561828 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2470 0.1535711510064506 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2113 0.1313748348488381 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1922 0.11949949483174012 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.217455506334033E-5 0.0 10-11 0.0 0.0 0.0 6.217455506334033E-5 0.0 12-13 0.0 0.0 0.0 6.217455506334033E-5 6.217455506334033E-5 14-15 0.0 0.0 0.0 6.217455506334033E-5 6.217455506334033E-5 16-17 0.0 0.0 0.0 6.217455506334033E-5 6.217455506334033E-5 18-19 0.0 0.0 0.0 2.4869822025336134E-4 6.217455506334033E-5 20-21 0.0 0.0 0.0 3.419600528483718E-4 6.217455506334033E-5 22-23 0.0 0.0 0.0 9.947928810134453E-4 6.217455506334033E-5 24-25 0.0 0.0 0.0 0.0017408875417735292 6.217455506334033E-5 26-27 0.0 0.0 0.0 0.0027667677003186445 6.217455506334033E-5 28-29 0.0 0.0 0.0 0.004383306131965493 9.32618325950105E-5 30-31 0.0 0.0 0.0 0.008269215823424263 1.8652366519002097E-4 32-33 0.0 0.0 0.0 0.016724955312038547 1.8652366519002097E-4 34-35 0.0 0.0 0.0 0.029688350042745008 1.8652366519002097E-4 36-37 0.0 0.0 0.0 0.05169814253516748 1.8652366519002097E-4 38-39 3.108727753167017E-5 0.0 0.0 0.08458848216367451 1.8652366519002097E-4 40-41 6.217455506334033E-5 0.0 0.0 0.1205564622678169 1.8652366519002097E-4 42-43 6.217455506334033E-5 0.0 0.0 0.15636900598430092 1.8652366519002097E-4 44-45 6.217455506334033E-5 0.0 0.0 0.2009792492422476 1.8652366519002097E-4 46-47 6.217455506334033E-5 0.0 0.0 0.25448045387425194 1.8652366519002097E-4 48-49 6.217455506334033E-5 0.0 0.0 0.3171213181005673 2.4869822025336134E-4 50-51 6.217455506334033E-5 0.0 0.0 0.38691225615916686 2.4869822025336134E-4 52-53 6.217455506334033E-5 0.0 0.0 0.45163596798010414 2.7978549778503146E-4 54-55 6.217455506334033E-5 0.0 0.0 0.5264941322763659 3.7304733038004195E-4 56-57 6.217455506334033E-5 0.0 0.0 0.6148130877438408 3.7304733038004195E-4 58-59 6.217455506334033E-5 0.0 0.0 0.704624232532836 3.7304733038004195E-4 60-61 6.217455506334033E-5 0.0 0.0 0.7927566643351209 4.0413460791171213E-4 62-63 6.217455506334033E-5 0.0 0.0 0.8800497396440508 4.352218854433823E-4 64-65 6.217455506334033E-5 0.0 0.0 0.9743996269526696 4.973964405067227E-4 66-67 1.2434911012668067E-4 0.0 0.0 1.0800652832828166 4.973964405067227E-4 68-69 1.2434911012668067E-4 0.0 6.217455506334033E-5 1.20037304733038 4.973964405067227E-4 70-71 1.2434911012668067E-4 0.0 6.217455506334033E-5 1.3499028522577134 5.284837180383928E-4 72-73 1.2434911012668067E-4 0.0 6.217455506334033E-5 1.5124893137483486 6.839201056967436E-4 74-75 1.2434911012668067E-4 0.0 6.217455506334033E-5 1.690122017564312 8.393564933550945E-4 76-77 1.2434911012668067E-4 0.0 6.217455506334033E-5 1.8979404678635268 8.704437708867646E-4 78-79 1.2434911012668067E-4 0.0 6.217455506334033E-5 2.1469806481697367 8.704437708867646E-4 80-81 1.2434911012668067E-4 0.0 6.217455506334033E-5 2.4258646149063496 9.32618325950105E-4 82-83 1.2434911012668067E-4 0.0 6.217455506334033E-5 2.7037848760394807 9.947928810134453E-4 84-85 1.2434911012668067E-4 0.0 6.217455506334033E-5 2.9757674671640633 9.947928810134453E-4 86-87 1.2434911012668067E-4 0.0 6.217455506334033E-5 3.261148674904795 9.947928810134453E-4 88-89 1.2434911012668067E-4 0.0 6.217455506334033E-5 3.573731250485739 0.0011191419911401258 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7890 0.0 35.66153 1 GTATCAA 11380 0.0 29.936737 1 ATCAACG 14620 0.0 23.030508 3 TATCAAC 14740 0.0 23.008175 2 GTACATG 9575 0.0 22.844646 1 TCAACGC 14880 0.0 22.631615 4 CAACGCA 15105 0.0 22.312712 5 TACATGG 9825 0.0 22.159184 2 AACGCAG 15405 0.0 21.939728 6 TGTCGCG 55 0.0026873867 21.847975 64-65 ACATGGG 9995 0.0 21.256306 3 GAGTACT 8770 0.0 20.88812 12-13 AGTACTT 9040 0.0 20.867195 12-13 GTACTTT 9340 0.0 19.461126 14-15 ACGCAGA 17515 0.0 19.242565 7 AGAGTAC 14745 0.0 19.239292 10-11 CATGGGA 6470 0.0 19.133585 4 CGCAGAG 17725 0.0 18.880867 8 AGTACAT 7635 0.0 18.326797 2 GAGTACA 8525 0.0 18.255836 1 >>END_MODULE