FastQCFastQC Report
Fri 27 May 2016
SRR939523_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939523_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2384156
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145250.6092302684891425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113090.4743397663575706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79440.33319967317574856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61700.2587917904700867No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC27410.11496730918614385No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT27390.11488342205795259No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA25470.1068302577515901No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG8700.040.0765889
GTATCAA180150.031.9546761
GTATCGA1451.4177203E-831.1244241
GGTATCA119400.030.616111
TCGACGC1503.0962838E-828.7229184
GTACATG103950.028.075361
TAGGCAT13300.025.7912065
TATCAAC222500.025.7752532
TACATGG111050.025.6017282
GGCATTC13350.025.47167
TCAACGC225500.025.3262674
ATCAACG226050.025.202543
CAACGCA225650.025.0932675
AACGCAG227300.024.8199846
ATCGACG1751.36768E-724.6417453
CCTTTAG15400.024.421221
ACATGGG110500.024.2391013
GAGTACT128050.023.89483312-13
CATGGGG32600.022.9078484
GACATAT11850.022.8508431
Produced by FastQC (version 0.11.5)