##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939523_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2384156 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.023273225409746 31.0 31.0 33.0 30.0 34.0 2 31.296255781920312 31.0 31.0 34.0 30.0 34.0 3 31.267435939594556 31.0 31.0 34.0 28.0 34.0 4 35.218668157620556 35.0 35.0 37.0 33.0 37.0 5 34.86820661064125 35.0 35.0 37.0 32.0 37.0 6 34.83159155692832 35.0 35.0 37.0 32.0 37.0 7 34.71062925412599 35.0 35.0 37.0 31.0 37.0 8 34.728925456220146 35.0 35.0 37.0 32.0 37.0 9 36.04226233518276 37.0 35.0 39.0 30.0 39.0 10-11 35.9230499178745 37.0 35.0 39.0 30.0 39.0 12-13 35.925835389966096 37.0 35.0 39.0 30.0 39.0 14-15 36.753317735919964 38.0 36.0 40.0 31.0 41.0 16-17 36.769054541733006 38.0 36.0 40.0 31.0 41.0 18-19 36.82354279669619 38.5 36.0 40.0 31.0 41.0 20-21 36.763256263432424 39.0 35.0 40.0 30.0 41.0 22-23 36.62690339893866 38.0 35.0 40.0 30.0 41.0 24-25 36.432179354035554 38.0 35.0 40.0 30.0 41.0 26-27 36.081904036480836 38.0 34.0 40.0 29.0 41.0 28-29 35.82620621301626 38.0 34.0 40.0 28.0 41.0 30-31 35.569405273815974 38.0 34.0 40.0 27.0 41.0 32-33 35.319716285343745 38.0 34.0 40.0 27.0 41.0 34-35 35.208853573339994 38.0 34.0 40.0 27.0 41.0 36-37 35.10736105355522 38.0 34.0 40.0 26.5 41.0 38-39 35.058983556445135 38.0 34.0 40.0 26.0 41.0 40-41 34.86844946387736 38.0 33.0 40.0 25.5 41.0 42-43 34.55255465665837 38.0 33.0 40.0 24.5 41.0 44-45 34.32391965961959 38.0 33.0 40.0 24.0 41.0 46-47 33.92691166182079 38.0 33.0 40.0 23.5 41.0 48-49 33.594228104201235 37.5 33.0 40.0 21.5 41.0 50-51 33.38000554493917 37.0 32.5 40.0 20.0 41.0 52-53 33.082896001771694 37.0 32.0 40.0 18.5 41.0 54-55 32.895567236372116 37.0 31.5 40.0 17.5 41.0 56-57 32.57002310251511 37.0 31.0 40.0 15.0 41.0 58-59 32.225595976102234 36.0 31.0 39.0 13.0 40.0 60-61 31.99543465276601 36.0 31.0 39.0 11.0 40.0 62-63 31.67086109298217 36.0 30.0 39.0 8.0 40.0 64-65 31.302443086777878 35.5 29.5 39.0 8.0 40.0 66-67 29.683481911418546 34.5 26.0 38.0 4.5 40.0 68-69 26.095038034423922 30.5 17.5 36.0 2.0 39.0 70-71 25.55828855158807 30.5 16.0 36.0 2.0 39.0 72-73 27.357634106157484 32.0 22.5 36.0 2.0 39.0 74-75 27.909349052662662 32.0 25.5 36.0 2.0 39.0 76-77 27.870312806712313 31.5 26.0 35.0 2.0 37.5 78-79 28.671483954908993 33.5 27.5 36.0 2.0 38.0 80-81 28.487128988203793 33.5 27.0 35.5 2.0 37.0 82-83 28.046044386357266 33.0 26.0 35.0 2.0 37.0 84-85 27.67918059892054 33.0 26.0 35.0 2.0 36.0 86-87 27.241895664545442 33.0 26.0 35.0 2.0 36.0 88-89 26.72787057558314 32.0 25.0 35.0 2.0 35.5 90-91 26.272454906474238 32.0 25.0 34.5 2.0 35.0 92-93 25.754870486662785 32.0 23.5 34.0 2.0 35.0 94-95 24.4137243955513 31.0 17.0 34.0 2.0 35.0 96-97 23.628621617041837 31.0 7.0 34.0 2.0 35.0 98-99 23.3487122906387 31.0 2.0 34.0 2.0 35.0 100-101 22.08608580982117 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.45716269826456823 1101 2 0.37913832758601274 1101 3 0.45900907898010246 1101 4 0.40420217563647753 1101 5 0.5359145459732915 1101 6 0.651281671188606 1101 7 0.6712914205049358 1101 8 0.568693734864226 1101 9 0.8040204320062827 1101 10-11 0.9855596246327778 1101 12-13 0.8475077553010522 1101 14-15 0.9988137843096396 1101 16-17 0.9567503573450367 1101 18-19 0.9818017747471117 1101 20-21 1.045257444260919 1101 22-23 0.9978855727933009 1101 24-25 0.9188557942928028 1101 26-27 0.9127650959453177 1101 28-29 1.0206992976467006 1101 30-31 1.1355789961943543 1101 32-33 1.1111092577805977 1101 34-35 1.227679054912386 1101 36-37 1.0660456716781113 1101 38-39 0.9568147699404577 1101 40-41 1.2398782988688808 1101 42-43 1.2689137118107539 1101 44-45 1.0224666587943574 1101 46-47 1.0674780442264122 1101 48-49 0.967880320731247 1101 50-51 0.9060688344495134 1101 52-53 1.0101110908244308 1101 54-55 0.7085351356753407 1101 56-57 0.8415906313723625 1101 58-59 0.9402407673395814 1101 60-61 0.8787152809060679 1101 62-63 0.9721300868755556 1101 64-65 0.7163575166676779 1101 66-67 -1.9648514831223878 1101 68-69 -7.642913884403352 1101 70-71 -11.17705789359422 1101 72-73 -10.910391339983132 1101 74-75 -8.833145595621335 1101 76-77 -3.6322913220809347 1101 78-79 -0.7374166075545787 1101 80-81 -0.019413891558272667 1101 82-83 0.21971288719612758 1101 84-85 0.15928820606918137 1101 86-87 0.2741624809109595 1101 88-89 0.3483394065451755 1101 90-91 0.29934198175080695 1101 92-93 -0.046467952637300414 1101 94-95 -3.5702299970724063 1101 96-97 -0.4991717668533191 1101 98-99 0.24849239965136505 1101 100-101 0.39027787228121724 1102 1 0.3793854350568395 1102 2 0.33229565374684356 1102 3 0.3428840880985895 1102 4 0.18654645992855734 1102 5 0.34181282031722304 1102 6 0.4143230707707133 1102 7 0.4030655763149298 1102 8 0.5792419668772055 1102 9 0.6961828944842878 1102 10-11 0.6551938470523524 1102 12-13 0.621988652493279 1102 14-15 0.6424436794977737 1102 16-17 0.6528860724717944 1102 18-19 0.8263360116654539 1102 20-21 0.8762640885710269 1102 22-23 0.7710822393479688 1102 24-25 0.6899253190631427 1102 26-27 0.7533372191430772 1102 28-29 0.6219523059897085 1102 30-31 0.5987707410378391 1102 32-33 0.5326780365757102 1102 34-35 0.6975615725159798 1102 36-37 0.8892910861779129 1102 38-39 0.7600415933269957 1102 40-41 0.9546745185345955 1102 42-43 1.1798037999505482 1102 44-45 0.8512374962328977 1102 46-47 0.8436666329393532 1102 48-49 0.7267757234715972 1102 50-51 0.7369384366475202 1102 52-53 0.8589061947812056 1102 54-55 0.7670547660687532 1102 56-57 0.6968376113758694 1102 58-59 0.5906977963513498 1102 60-61 0.6010253583972833 1102 62-63 0.7539537473513427 1102 64-65 0.7562658293652369 1102 66-67 -1.9802812444617715 1102 68-69 -7.788846253553487 1102 70-71 -11.079236310770689 1102 72-73 -9.038368846612766 1102 74-75 -6.887266862831812 1102 76-77 -3.418860453507879 1102 78-79 -0.8877442373329316 1102 80-81 -0.14359984930480607 1102 82-83 -0.001006487788924204 1102 84-85 -0.0950816287447509 1102 86-87 -0.11096187402250735 1102 88-89 0.028689398932566945 1102 90-91 -0.14368285472942688 1102 92-93 -0.4303029312327027 1102 94-95 -3.78323793446679 1102 96-97 -0.7527605902707357 1102 98-99 -0.1866735900529939 1102 100-101 -6.157444700605197E-5 1103 1 0.3129474235387377 1103 2 0.17626607437630426 1103 3 0.33179893628117085 1103 4 0.2026918959824613 1103 5 0.38924453937365655 1103 6 0.3610327302950225 1103 7 0.44704968780476406 1103 8 0.40220758503733123 1103 9 0.7041751564811278 1103 10-11 0.6657446997084691 1103 12-13 0.4536568571591957 1103 14-15 0.6551112814102211 1103 16-17 0.6073950773240497 1103 18-19 0.5549853816191685 1103 20-21 0.5539536758716039 1103 22-23 0.6755009200824418 1103 24-25 0.7589076969700059 1103 26-27 0.8545922443111422 1103 28-29 0.804173656975351 1103 30-31 0.7426158991850542 1103 32-33 0.7149553956265251 1103 34-35 0.7365200714287141 1103 36-37 0.7736491003986288 1103 38-39 0.7247302862765608 1103 40-41 0.7458280001915085 1103 42-43 0.8271685760338627 1103 44-45 0.710986188933532 1103 46-47 0.626839409819226 1103 48-49 0.4423721026835423 1103 50-51 0.4333222714752907 1103 52-53 0.5449494294514423 1103 54-55 0.5235685929487133 1103 56-57 0.5120883408142376 1103 58-59 0.36253027356139 1103 60-61 0.32648893626361186 1103 62-63 0.276792591700481 1103 64-65 0.2801212645479012 1103 66-67 -2.7013335204339555 1103 68-69 -7.647852617136511 1103 70-71 -11.674627824926507 1103 72-73 -9.028364294846195 1103 74-75 -7.221750139534766 1103 76-77 -3.768969535882409 1103 78-79 -1.0260121479120556 1103 80-81 -0.49914615908452475 1103 82-83 -0.10690484826394808 1103 84-85 -0.21303594749998567 1103 86-87 -0.050619851437922136 1103 88-89 0.012426168138443217 1103 90-91 -0.12048618107508702 1103 92-93 0.07889883826655364 1103 94-95 1.0331516256298663 1103 96-97 1.2356506298338452 1103 98-99 0.8135491086822917 1103 100-101 0.7749158000727014 1104 1 0.3946727237005305 1104 2 0.18797649731696353 1104 3 0.3302786619983529 1104 4 0.08139459526112347 1104 5 0.38915744032620125 1104 6 0.528160406061339 1104 7 0.4111138525292972 1104 8 0.2818886089702133 1104 9 0.6567413671534865 1104 10-11 0.5666937274103674 1104 12-13 0.6503841324458506 1104 14-15 0.6236511494595689 1104 16-17 0.6612603394965788 1104 18-19 0.7278202444645672 1104 20-21 0.7977634849839248 1104 22-23 0.5536651888859367 1104 24-25 0.6395083196842037 1104 26-27 0.7495866444582475 1104 28-29 0.8557219965068015 1104 30-31 0.7970107939491697 1104 32-33 0.651451612096686 1104 34-35 0.7810232857354791 1104 36-37 0.7181929609275386 1104 38-39 0.7455333218983071 1104 40-41 0.8413560799566397 1104 42-43 1.022028280583335 1104 44-45 0.8498522033251135 1104 46-47 0.6306911232510473 1104 48-49 0.7169872612609893 1104 50-51 0.6088690819345643 1104 52-53 0.7079005299347116 1104 54-55 0.6161203486983524 1104 56-57 0.7121511137135954 1104 58-59 0.6756940188908231 1104 60-61 0.5962793354851748 1104 62-63 0.6184518021608163 1104 64-65 0.5143262042076202 1104 66-67 -2.520563626439653 1104 68-69 -7.356316269120388 1104 70-71 -8.813161939159986 1104 72-73 -7.875821090639402 1104 74-75 -6.6259163333151925 1104 76-77 -3.2849185591853605 1104 78-79 -0.2871542275733745 1104 80-81 0.3952750589472629 1104 82-83 0.4244431106710529 1104 84-85 0.38881913024251347 1104 86-87 0.5613024245393667 1104 88-89 0.4869679523668289 1104 90-91 0.30232096843703005 1104 92-93 0.6667905750303547 1104 94-95 1.669260163360029 1104 96-97 1.7528148608064456 1104 98-99 1.5237444041020218 1104 100-101 1.2723316417178339 1105 1 0.48101557003323236 1105 2 0.3954859424121224 1105 3 0.47436006117983354 1105 4 0.28777001772443356 1105 5 0.4131973188052527 1105 6 0.555498297250324 1105 7 0.526220802029286 1105 8 0.5170576516878498 1105 9 0.7448676120329054 1105 10-11 0.8535129765463552 1105 12-13 0.7796334935425406 1105 14-15 0.9642178834937667 1105 16-17 1.0250071550426654 1105 18-19 0.8232670996201961 1105 20-21 1.0018094427902824 1105 22-23 0.9596923991678992 1105 24-25 1.0725862258613077 1105 26-27 0.9789087696467647 1105 28-29 1.0960331990314387 1105 30-31 0.9929748093403958 1105 32-33 1.172728735951729 1105 34-35 1.121698398165016 1105 36-37 1.0965616171204147 1105 38-39 0.9278760960781298 1105 40-41 0.8617486601957864 1105 42-43 1.0336106066460715 1105 44-45 1.0236339787301958 1105 46-47 1.038032142058647 1105 48-49 0.9169614263560248 1105 50-51 0.8885039019004637 1105 52-53 1.0967771945754308 1105 54-55 1.0155231345448783 1105 56-57 1.0751733444033462 1105 58-59 1.2429226879131932 1105 60-61 1.183662584597915 1105 62-63 1.284791097498502 1105 64-65 1.3403362438931907 1105 66-67 2.083002365642315 1105 68-69 -5.142557102611367 1105 70-71 -4.658002166000291 1105 72-73 -1.0970345142353928 1105 74-75 1.350829494302932 1105 76-77 1.381498777541541 1105 78-79 1.3451004351201163 1105 80-81 1.3229471640626826 1105 82-83 1.391314280064993 1105 84-85 1.2663077896435055 1105 86-87 1.555156162006348 1105 88-89 1.5064932072908555 1105 90-91 1.4632675814183749 1105 92-93 1.5951763824397105 1105 94-95 2.3817231868727724 1105 96-97 2.490644242800631 1105 98-99 2.1113458262293143 1105 100-101 2.103466548371536 1106 1 0.10687572254712663 1106 2 0.08584654729773789 1106 3 0.1292386224531228 1106 4 -0.01034242896032822 1106 5 0.18212761663202315 1106 6 0.16698107942872298 1106 7 0.20119766644702253 1106 8 0.15028647792171768 1106 9 0.410045920347315 1106 10-11 0.3400103638688705 1106 12-13 0.3622452847499176 1106 14-15 0.2691971482512443 1106 16-17 0.1015118910976085 1106 18-19 0.17824832157776171 1106 20-21 0.27541493772187664 1106 22-23 0.38420267446840484 1106 24-25 0.38280199793982206 1106 26-27 0.43000011180596687 1106 28-29 0.5044959845333779 1106 30-31 0.364657742924841 1106 32-33 0.4942573461550168 1106 34-35 0.5890505280049325 1106 36-37 0.5234309463177738 1106 38-39 0.5660304933158997 1106 40-41 0.5526596445197214 1106 42-43 0.6084023125490603 1106 44-45 0.5047717397671363 1106 46-47 0.36749147476420063 1106 48-49 0.1893231760190517 1106 50-51 0.10126255697163344 1106 52-53 0.42933931721432117 1106 54-55 0.30589636891036065 1106 56-57 0.43612299266124666 1106 58-59 0.5241063877599359 1106 60-61 0.4626399660118956 1106 62-63 0.5200494294470239 1106 64-65 0.4921665952660028 1106 66-67 1.250166751986228 1106 68-69 -5.537854578561809 1106 70-71 -6.076759676294056 1106 72-73 -2.483072587316503 1106 74-75 -0.06821347177842085 1106 76-77 0.006049969402543098 1106 78-79 -0.06036694795564301 1106 80-81 0.05868297897801966 1106 82-83 0.08737890459646636 1106 84-85 0.19567561401249023 1106 86-87 0.2163401572795216 1106 88-89 0.05627470251787159 1106 90-91 -0.02398122302779626 1106 92-93 0.09859372766396035 1106 94-95 1.0174227381938827 1106 96-97 0.913928498418354 1106 98-99 0.5749794236000163 1106 100-101 0.5382203658349312 1107 1 -0.12435841816798288 1107 2 -0.09037798172956002 1107 3 -0.016511034272312486 1107 4 -0.039712671174861214 1107 5 0.11195636381717122 1107 6 0.14253949102865704 1107 7 0.07547371412098869 1107 8 0.035444933460794914 1107 9 0.09388092886052135 1107 10-11 0.11667450403474078 1107 12-13 0.07887317512174974 1107 14-15 0.04771881574176717 1107 16-17 0.02688894387950569 1107 18-19 0.2766476185514577 1107 20-21 0.14243860993585855 1107 22-23 0.10345373175406536 1107 24-25 0.3032914800071609 1107 26-27 0.2549691896331723 1107 28-29 0.2321156912395992 1107 30-31 0.20992890236900763 1107 32-33 0.1710594552339444 1107 34-35 0.3162020631287845 1107 36-37 0.26759703628317766 1107 38-39 0.2203506998298721 1107 40-41 0.22178668357064169 1107 42-43 0.250978684274223 1107 44-45 0.07438123062230773 1107 46-47 -0.04366489366552884 1107 48-49 0.2602398564167032 1107 50-51 0.2726522422575144 1107 52-53 0.3799497417763078 1107 54-55 0.16494772278524295 1107 56-57 0.4526355427793405 1107 58-59 0.30679744065167824 1107 60-61 0.28005480645787273 1107 62-63 0.4025732938947648 1107 64-65 0.3247837205474866 1107 66-67 1.1453341052020214 1107 68-69 -5.930518499031958 1107 70-71 -5.624580624281094 1107 72-73 -1.8737065193206774 1107 74-75 0.13379147137531433 1107 76-77 0.09762661574735532 1107 78-79 0.1680553072209392 1107 80-81 0.05463684287652271 1107 82-83 0.1768460326241481 1107 84-85 0.23522435900068572 1107 86-87 0.3513831627169921 1107 88-89 0.23360386163249913 1107 90-91 0.29693842326209463 1107 92-93 0.3165406711472336 1107 94-95 1.1094754651670264 1107 96-97 1.2922227808372035 1107 98-99 0.9645364071777642 1107 100-101 0.7977064975425172 1108 1 0.050978286649691285 1108 2 0.02700039345158345 1108 3 0.03357561879012039 1108 4 0.058042186424287934 1108 5 0.023090986595391882 1108 6 0.08250488895253483 1108 7 0.06806946131881375 1108 8 0.03226449989973901 1108 9 0.12391405221544005 1108 10-11 0.2358931477516535 1108 12-13 0.21692843493305958 1108 14-15 0.19242425447834677 1108 16-17 0.11447892406464888 1108 18-19 0.28646810179754567 1108 20-21 0.2856347179416545 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58-59 0.35100519728374024 2311 60-61 0.42613996601189186 2311 62-63 0.44027561992321296 2311 64-65 0.4337439762183841 2311 66-67 1.5859881805576563 2311 68-69 4.041889469057235 2311 70-71 4.4079188951345145 2311 72-73 2.301254793635877 2311 74-75 1.481167480602533 2311 76-77 1.068930921783494 2311 78-79 0.6247878139491192 2311 80-81 0.5440579789780244 2311 82-83 0.31992057126313256 2311 84-85 0.2939553759172533 2311 86-87 0.34272110966047364 2311 88-89 0.3384354168035877 2311 90-91 0.21468544363887432 2311 92-93 0.31921872766395865 2311 94-95 1.12454178581293 2311 96-97 1.4004523079421638 2311 98-99 1.0800746616952601 2311 100-101 1.0568275086920735 2312 1 -0.14202999225366497 2312 2 -0.05727946676391582 2312 3 -0.05684415073186955 2312 4 0.029134044809275395 2312 5 -0.030599365778030574 2312 6 0.23412738760045215 2312 7 0.1448111512135597 2312 8 0.1699048044302529 2312 9 -1.2799628875024922 2312 10-11 -0.8709987272743476 2312 12-13 -1.6744291596086924 2312 14-15 -1.7488880973126442 2312 16-17 0.0541627448409443 2312 18-19 -1.8279987617877609 2312 20-21 -1.6409413938859458 2312 22-23 -0.3685674715564673 2312 24-25 -1.1012912313475667 2312 26-27 -1.1393664956266178 2312 28-29 0.11198997098680508 2312 30-31 0.4170063483486217 2312 32-33 0.4287519764571144 2312 34-35 0.7835071454815647 2312 36-37 0.9093822713583393 2312 38-39 0.7254004487615688 2312 40-41 1.0550473444347332 2312 42-43 0.8234899017220982 2312 44-45 0.8604172624434909 2312 46-47 0.9172448034618199 2312 48-49 1.1849182209596947 2312 50-51 0.9218148246066562 2312 52-53 1.1379490676585817 2312 54-55 1.2419121303257938 2312 56-57 1.092976581473735 2312 58-59 1.273629476173241 2312 60-61 1.438178455285378 2312 62-63 0.9663489478482141 2312 64-65 1.3827926737125296 2312 66-67 2.5985600662616406 2312 68-69 4.842543989518749 2312 70-71 5.541577854932605 2312 72-73 3.58457314139142 2312 74-75 2.8413306318554596 2312 76-77 1.676955529679656 2312 78-79 1.4941219134623722 2312 80-81 1.5012955623564253 2312 82-83 1.7830320504410686 2312 84-85 1.6394343510389433 2312 86-87 1.7899736786166685 2312 88-89 1.717914301786461 2312 90-91 1.8208112774229015 2312 92-93 1.8998124044261715 2312 94-95 2.4931081279798697 2312 96-97 2.8151386248702295 2312 98-99 2.6246564175993505 2312 100-101 2.363995121816288 2313 1 -0.24484044907003266 2313 2 -0.12662870022701256 2313 3 -0.1531951352083567 2313 4 -0.01273846856428662 2313 5 -0.03954800807330372 2313 6 0.07804944340797704 2313 7 -3.3294095942790527 2313 8 -1.061764441455935 2313 9 -0.5422397939383998 2313 10-11 -0.006502130237684867 2313 12-13 -1.2190102550897848 2313 14-15 -0.2208278399618635 2313 16-17 0.019178086288555107 2313 18-19 0.3191337529780469 2313 20-21 0.21993403248849575 2313 22-23 -0.987114157214755 2313 24-25 -2.3135294494906375 2313 26-27 -2.022352080556132 2313 28-29 -0.32880623140249554 2313 30-31 0.06635788436755519 2313 32-33 -2.2514484529963283 2313 34-35 -2.258406331966782 2313 36-37 -0.68373737051391 2313 38-39 -0.26646644209193227 2313 40-41 -0.22029509381125223 2313 42-43 -0.3301106530332092 2313 44-45 0.0644322772494661 2313 46-47 -0.057059208875593015 2313 48-49 -0.08905259012899336 2313 50-51 -0.003911417568673414 2313 52-53 0.24905501735576507 2313 54-55 0.456494671597774 2313 56-57 0.4040423703132987 2313 58-59 0.1956731015741724 2313 60-61 0.6698610881241045 2313 62-63 0.2925322205832792 2313 64-65 0.1405777220929707 2313 66-67 1.6673454412837287 2313 68-69 4.478483941004431 2313 70-71 4.636652393484351 2313 72-73 2.7338104867051847 2313 74-75 1.7933787017246452 2313 76-77 1.0913319118825058 2313 78-79 0.720468507018424 2313 80-81 0.7436763783179572 2313 82-83 0.5001247791839241 2313 84-85 0.6434912670063646 2313 86-87 0.538315169066415 2313 88-89 0.6019234531072151 2313 90-91 0.7297679518896985 2313 92-93 0.9267785461458047 2313 94-95 1.5386094425786077 2313 96-97 1.4362938921005757 2313 98-99 1.280751229352024 2313 100-101 0.9821699179329997 2314 1 -0.25688701398853553 2314 2 -0.16007048326994777 2314 3 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-1.4994751078788262 2314 64-65 -0.7301597719621107 2314 66-67 -0.4546431877101753 2314 68-69 1.7275235665827076 2314 70-71 1.8683901469540203 2314 72-73 -0.23709400549076065 2314 74-75 0.0554214835137401 2314 76-77 -0.2040494275614826 2314 78-79 0.13215135106702292 2314 80-81 0.5885221347276577 2314 82-83 0.22698661929806718 2314 84-85 -0.002810453777069455 2314 86-87 -0.11718063706441484 2314 88-89 0.07530859729849126 2314 90-91 0.37075167362431927 2314 92-93 0.4350165806479467 2314 94-95 0.39191805946649794 2314 96-97 0.7701793821779681 2314 98-99 0.16951425412611698 2314 100-101 0.16486426269498722 2315 1 -0.38769564784586663 2315 2 -0.29003616498081897 2315 3 -0.3609293386836825 2315 4 -0.18523796958341876 2315 5 -1.0524794753189894 2315 6 -0.3887535105587645 2315 7 -0.2910621584358992 2315 8 -0.14808510773148242 2315 9 -0.21887388068244462 2315 10-11 -1.7149634849253772 2315 12-13 -1.2762256696672836 2315 14-15 -0.25453430010085754 2315 16-17 -1.783724167895322 2315 18-19 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84-85 -4.753828298487143 2315 86-87 -5.256784082764693 2315 88-89 -4.026031723290082 2315 90-91 -3.7094970039278152 2315 92-93 -4.800473672050966 2315 94-95 -2.9216427998111776 2315 96-97 -1.67754708118429 2315 98-99 -3.0135244016319653 2315 100-101 -2.0995227763822477 2316 1 -0.3362646991666445 2316 2 -0.27819113776282833 2316 3 -0.7498843667156265 2316 4 -0.34904498616086954 2316 5 -0.21154872766074106 2316 6 -2.2506714232951097 2316 7 -0.3888122034497954 2316 8 -0.2546471886808632 2316 9 0.14975213069147486 2316 10-11 0.06071766374709853 2316 12-13 -0.47415630468802306 2316 14-15 -0.8257220339573124 2316 16-17 -1.2013394836380158 2316 18-19 -0.02477840405192211 2316 20-21 -3.6226911957140047 2316 22-23 -3.7106883080349533 2316 24-25 -2.4213986045084184 2316 26-27 -1.545982199288055 2316 28-29 -0.8510934533952224 2316 30-31 0.05446470312480045 2316 32-33 -1.5663179278307595 2316 34-35 -2.0471994239273883 2316 36-37 -4.126410238069845 2316 38-39 -1.8884858176499435 2316 40-41 -1.1402113418290583 2316 42-43 -2.307197123456831 2316 44-45 -0.3205330093195755 2316 46-47 -0.47656069469039863 2316 48-49 0.07334996573897712 2316 50-51 0.24704336835406338 2316 52-53 -2.2763034437931715 2316 54-55 -0.5051276649292902 2316 56-57 0.5171731753184332 2316 58-59 0.6206118033851382 2316 60-61 -0.988757970282407 2316 62-63 -0.14814912211357623 2316 64-65 0.7094222512296007 2316 66-67 1.6770172294585066 2316 68-69 4.0211354874511365 2316 70-71 4.085731254937119 2316 72-73 2.3475056348202656 2316 74-75 1.442484214563951 2316 76-77 0.18725191774581162 2316 78-79 0.5636051087001306 2316 80-81 0.2695865231682433 2316 82-83 0.3423658942779362 2316 84-85 0.48360460202761857 2316 86-87 0.01202124424997919 2316 88-89 0.5992746384276337 2316 90-91 0.7146899299556075 2316 92-93 0.957283723177639 2316 94-95 1.5751081833005927 2316 96-97 1.6276191989246662 2316 98-99 0.6233967792367565 2316 100-101 0.5310362345781243 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 13.0 4 49.0 5 154.0 6 420.0 7 1034.0 8 2098.0 9 3655.0 10 6432.0 11 9762.0 12 12949.0 13 16239.0 14 19487.0 15 25718.0 16 30669.0 17 35309.0 18 29207.0 19 22566.0 20 21271.0 21 21469.0 22 23338.0 23 26738.0 24 31964.0 25 39763.0 26 50202.0 27 62116.0 28 77168.0 29 93229.0 30 110825.0 31 130104.0 32 151689.0 33 177549.0 34 209040.0 35 247731.0 36 276991.0 37 270856.0 38 137014.0 39 9336.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.20956087647661 25.196961388897165 16.69654458046249 20.89693315416374 2 13.99183446072369 23.798997850378996 41.26399728822206 20.94517040067526 3 15.918547276268836 29.331553807720635 34.370821372426974 20.379077543583556 4 10.709198559154686 21.307246673455932 41.916300778975874 26.06725398841351 5 9.905991771501242 41.16774384865401 37.43631386072958 11.489950519115174 6 24.67447599905375 43.38885542724553 19.213382010237584 12.723286563463128 7 23.2681921820552 37.13641221463696 24.014032638803837 15.581362964504 8 23.523334882448967 37.68457265380285 21.41739047277108 17.3747019909771 9 24.84162110197487 17.586600876788264 24.3845620840247 33.18721593721216 10-11 21.52084427361297 29.783390851940894 30.344134360335485 18.351630514110653 12-13 22.60923781833068 28.73050253423014 30.165748382236735 18.494511265202444 14-15 20.63933735879699 29.644872231515052 29.095705146810865 20.620085262877094 16-17 17.463268833675105 32.70366718159576 30.49314912209692 19.339914862632213 18-19 17.23100902131258 30.796595993845976 34.31762586970856 17.654769115132886 20-21 18.156160148983602 29.910597255631448 34.34699086259073 17.58625173279422 22-23 18.063604059465906 30.09922588958105 33.70232484787069 18.134845203082346 24-25 17.688838314271383 30.85156759876451 34.05439912488948 17.40519496207463 26-27 17.722379895698523 30.31105680796042 34.589652942887525 17.376910353453532 28-29 17.118003182795537 30.2593997240067 34.87898392178554 17.74361317141223 30-31 17.985993242210203 29.944462469225336 34.57416297846094 17.49538131010353 32-33 17.853043310656215 30.1805870826219 34.3415586030807 17.624811003641188 34-35 18.10272722239094 30.31291579906408 34.069683938180276 17.514673040364702 36-37 18.521494913513568 29.564769410795556 33.900742451934605 18.01299322375628 38-39 17.596080508541295 30.917787377623483 33.59859222790389 17.88753988593133 40-41 17.605220368136905 30.30003090764326 34.46174927182265 17.63299945239719 42-43 17.583705363000533 30.289768510597202 34.079715994319045 18.046810132083216 44-45 18.09577991088532 30.282931232648092 33.515075495562634 18.106213360903954 46-47 18.425058460742658 30.682343249739212 32.502241546206086 18.390356743312047 48-49 17.923579820222145 30.687085465335862 32.752933193906856 18.636401520535138 50-51 17.861080600845106 30.72376089132523 32.510311910088205 18.904846597741457 52-53 18.080863583682547 31.58176755759974 32.2019981874347 18.135370671283013 54-55 18.51085387571862 30.992758486522426 31.791012263102026 18.705375374656928 56-57 18.446448360056948 31.47582076876566 31.581769729479785 18.49596114169761 58-59 18.218967409361376 30.790094774959808 32.017193902721424 18.97374391295739 60-61 18.57015995476366 31.39483473466436 31.669319156147598 18.365686154424385 62-63 18.351888770559498 30.76067575542643 32.240762512913584 18.64667296110048 64-65 18.352434920134904 30.914181174358017 31.856904097919024 18.876479807588048 66-67 18.148199393188523 30.978953661917934 31.487164065068285 19.385682879825257 68-69 18.528743019446715 30.202183672455472 31.735067191954514 19.5340061161433 70-71 18.27120202670578 30.724236700378093 32.398516068134924 18.606045204781203 72-73 19.08419004484539 30.160794817417607 31.687207834104868 19.06780730363213 74-75 18.025127191257045 31.15457505213566 31.51022539364022 19.31007236296708 76-77 18.06121209744049 31.138099839604394 31.645819992377692 19.15486807057742 78-79 18.114601230905528 30.8760386184864 31.290206616284998 19.71915353432307 80-81 17.93708486980397 30.83116953387135 31.28968320130329 19.942062395021384 82-83 18.092272485525278 31.473842315687396 31.077223973599043 19.356661225188283 84-85 17.841869407874317 31.05784604698686 30.953322685260527 20.146961859878296 86-87 17.564396572786347 31.017793534599818 31.523948237371563 19.89386165524227 88-89 18.401401931682674 30.56885525550314 31.283788403276823 19.745954409537365 90-91 17.90914370287847 30.689980103281734 31.690960992407284 19.709915201432512 92-93 18.210675880437687 30.93994096335638 31.466405213680144 19.382977942525784 94-95 17.907922422977755 30.372724580468617 31.455547570920615 20.263805425633006 96-97 18.158534767983983 30.11257191417235 31.123947232847954 20.604946084995714 98-99 17.829257370345783 30.357004647006818 30.81591623431989 20.997821748327507 100-101 17.723754913023235 30.38672885984304 31.258992353091692 20.63052387404204 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6454.0 1 4167.0 2 1898.0 3 1915.5 4 1706.0 5 1772.0 6 2835.0 7 5103.0 8 7260.5 9 7975.0 10 7048.5 11 5680.5 12 5120.0 13 4784.5 14 4592.5 15 4578.5 16 4663.0 17 4980.5 18 5542.0 19 6183.0 20 7137.5 21 8376.0 22 9848.0 23 11689.5 24 13703.5 25 18212.0 26 27556.0 27 36846.5 28 40041.5 29 43808.0 30 52680.5 31 63577.5 32 77772.5 33 94959.0 34 112559.0 35 122515.0 36 131950.0 37 143446.0 38 139758.5 39 128009.5 40 117401.5 41 107313.5 42 99670.5 43 93619.0 44 87088.5 45 79653.5 46 70792.5 47 62041.0 48 58727.5 49 51570.0 50 39185.5 51 29639.0 52 22704.0 53 18204.0 54 15006.5 55 12430.0 56 10152.5 57 8260.0 58 6628.0 59 5322.0 60 4057.0 61 2894.0 62 1969.5 63 1232.5 64 803.0 65 550.0 66 406.5 67 310.0 68 216.0 69 152.5 70 104.5 71 80.0 72 78.5 73 73.5 74 76.0 75 74.0 76 48.5 77 32.5 78 25.0 79 23.5 80 21.5 81 16.5 82 10.5 83 6.5 84 4.5 85 1.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02378200084222677 2 0.02013291076590626 3 0.0 4 0.0 5 1.258306922869141E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.774920768607423E-4 18-19 0.0010066455382953129 20-21 4.1943564095638034E-5 22-23 0.0 24-25 0.0 26-27 3.984638589085614E-4 28-29 0.01688228454849431 30-31 0.0038797796788465184 32-33 0.007612756883358303 34-35 0.008053164306362503 36-37 0.10288756272660012 38-39 0.10571875330305568 40-41 0.12029414182628988 42-43 0.10542514835438621 44-45 0.10039192066290964 46-47 0.10190188897035261 48-49 0.10462822063656907 50-51 0.10251007064973937 52-53 0.07956694108942536 54-55 0.0689132758091333 56-57 0.03913334530123029 58-59 0.016945199894637768 60-61 0.009730906870188025 62-63 0.0016357989997298832 64-65 0.004383102447994175 66-67 0.4170238860208812 68-69 0.8012688767010213 70-71 0.8854076662768712 72-73 0.27909247549237554 74-75 0.15047253619310147 76-77 0.01520454198466879 78-79 0.00922758410104037 80-81 0.0016567707817777026 82-83 0.0 84-85 0.0 86-87 1.4680247433473313E-4 88-89 7.130405896258466E-4 90-91 0.0039636668070377945 92-93 0.0018245450381602546 94-95 0.2215249337711123 96-97 0.22504819315514588 98-99 0.015246485548764427 100-101 0.007801502921788675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2384156.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.971017701927007 #Duplication Level Percentage of deduplicated Percentage of total 1 66.41905785250181 15.921324115276347 2 13.54301864231052 6.49279879224706 3 6.049442698261511 4.350338940204592 4 3.4104234124209523 3.2700527996083584 5 2.128964614246581 2.5516724227440495 6 1.5030848732448314 2.161828446243034 7 1.0759831231161148 1.8054687343833766 8 0.7804196980386549 1.49659635172937 9 0.6028268622838313 1.3005336048302552 >10 3.6571226035292885 16.429997427406942 >50 0.3796599068965651 6.431605483691033 >100 0.38073827127122056 18.76019758363709 >500 0.0473677840825597 7.772483633989359 >1k 0.021171964097507743 8.741361363136791 >5k 1.7942342455515037E-4 0.3896241553844983 >10k+ 5.382702736654511E-4 2.124116145487826 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19330 0.8107690939686832 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17493 0.7337187667249963 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12564 0.5269789392975963 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9059 0.379966747142385 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 4329 0.18157368897001705 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 4083 0.1712555722024901 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3808 0.15972109207618965 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3713 0.15573645348710405 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 3505 0.14701219215521133 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 3374 0.14151758525868274 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 3258 0.1366521318235887 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 2820 0.11828085074969925 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 2805 0.11765169728826469 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2774 0.11635144680129991 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 2626 0.11014379931514548 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 2619 0.10985019436647603 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 2617 0.10976630723828473 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 2580 0.10821439536674614 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 2536 0.10636887854653806 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 2467 0.10347477262393903 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 2466 0.1034328290598434 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 2415 0.10129370729096586 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 2406 0.10091621521410511 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.194356409563804E-5 0.0 12-13 0.0 0.0 0.0 2.3068960252600922E-4 0.0 14-15 0.0 0.0 0.0 3.145767307172853E-4 0.0 16-17 0.0 0.0 0.0 6.291534614345706E-4 0.0 18-19 0.0 0.0 0.0 7.969277178171227E-4 0.0 20-21 0.0 0.0 0.0 0.001111504448534408 0.0 22-23 0.0 0.0 0.0 0.0018455168202080737 0.0 24-25 0.0 0.0 0.0 0.0026634163200730154 0.0 26-27 0.0 0.0 0.0 0.004173384627515984 0.0 28-29 0.0 0.0 0.0 0.007843446485884312 0.0 30-31 0.0 0.0 0.0 0.01767921226631143 0.0 32-33 0.0 0.0 0.0 0.03640701363501382 0.0 34-35 0.0 0.0 0.0 0.06364935851513072 0.0 36-37 0.0 0.0 0.0 0.10710289091821174 0.0 38-39 0.0 0.0 0.0 0.16987143458733406 0.0 40-41 0.0 0.0 0.0 0.239057343563089 0.0 42-43 0.0 0.0 0.0 0.3078028451158398 0.0 44-45 0.0 0.0 0.0 0.39343063121708477 0.0 46-47 0.0 0.0 0.0 0.4923335553545993 0.0 48-49 0.0 0.0 0.0 0.5952001462991516 0.0 50-51 0.0 0.0 0.0 0.7091398381649523 0.0 52-53 0.0 0.0 0.0 0.818570596890472 0.0 54-55 0.0 0.0 0.0 0.9433107565108995 0.0 56-57 0.0 0.0 0.0 1.0863173382949773 0.0 58-59 0.0 0.0 0.0 1.2234308493236181 0.0 60-61 0.0 0.0 0.0 1.3629351435057102 0.0 62-63 0.0 0.0 0.0 1.49784661741933 2.097178204781902E-5 64-65 0.0 0.0 0.0 1.6432439823568592 4.194356409563804E-5 66-67 0.0 0.0 0.0 1.79612827348546 4.194356409563804E-5 68-69 0.0 0.0 0.0 1.9778487649298118 4.194356409563804E-5 70-71 0.0 0.0 0.0 2.228482532183297 4.194356409563804E-5 72-73 0.0 0.0 0.0 2.486309620679184 4.194356409563804E-5 74-75 0.0 0.0 0.0 2.7699110293118405 4.194356409563804E-5 76-77 0.0 0.0 0.0 3.091072899592141 4.194356409563804E-5 78-79 0.0 0.0 0.0 3.4515778329941496 4.194356409563804E-5 80-81 0.0 0.0 0.0 3.8496641998258503 4.194356409563804E-5 82-83 0.0 0.0 0.0 4.243367464209557 4.194356409563804E-5 84-85 0.0 0.0 0.0 4.629835463786765 4.194356409563804E-5 86-87 0.0 0.0 0.0 5.047027124064029 4.194356409563804E-5 88-89 0.0 0.0 0.0 5.4888606282474806 4.194356409563804E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 895 0.0 40.22467 9 GGTATCA 12675 0.0 33.313705 1 GTATCAA 18545 0.0 29.847588 1 TAGGCAT 1365 0.0 27.41551 5 GGCATTC 1355 0.0 26.918655 7 GACATAT 1240 0.0 26.75282 1 TTAGGCA 1420 0.0 26.353643 4 GCGTCGA 65 8.920475E-6 25.966171 64-65 CCTTTAG 1450 0.0 25.819763 1 TGGGCGA 185 9.244104E-9 25.605318 6 GTACATG 11480 0.0 25.511723 1 CATATAG 1390 0.0 25.218475 3 TACATGG 11970 0.0 23.868008 2 TCAACGC 23085 0.0 23.802908 4 TACGTAC 100 0.0016764904 23.695356 1 TATCAAC 23360 0.0 23.649323 2 ATCAACG 23400 0.0 23.583702 3 CAACGCA 23365 0.0 23.47711 5 GCATTCC 1580 0.0 23.38511 8 ACATGGG 11675 0.0 23.248753 3 >>END_MODULE