FastQCFastQC Report
Fri 27 May 2016
SRR939522_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939522_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2393835
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146170.6106101715448224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107400.4486524760478479No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82010.34258835717582875No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63490.2652229581403898No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT28000.11696712597150598No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA26360.11011619430746063No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC25060.10468557774449784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG9050.043.4422079
TCGACGC1451.6370905E-1136.170314
ATCGACG1502.1827873E-1135.2907873
GGTATCA116550.033.042541
GTATCAA177350.032.6573681
TAGGCAT12850.030.2411465
GGCATTC14000.028.4271457
CCTTTAG14200.028.398411
CGACGCA1954.129106E-1027.4011945
TTAGGCA14800.027.0610794
GTACATG98950.026.6936451
TATCAAC221300.025.943952
GTATCGA1758.371899E-825.9236931
TCAACGC226550.025.6757584
ATCAACG228000.025.5393853
CAACGCA226000.025.211635
AACGCAG227250.024.902456
TACATGG105700.024.7278162
AACGCCG3000.024.2670766
GCATTCC16950.024.0533568
Produced by FastQC (version 0.11.5)