FastQCFastQC Report
Fri 27 May 2016
SRR939522_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939522_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2393835
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198570.8295057930057836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167790.7009255023842496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125650.524889977797133No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90310.377260755231668No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT43740.18271936035691683No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA39550.16521606543475217No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38010.15878287350631937No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36140.15097114045036522No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC35240.1472114828298525No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT32550.1359742839418757No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT32370.13522235241777317No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA27610.11533794100261714No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27170.11349988616592205No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC26770.11182892722347196No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA26180.10936426278335809No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT26170.10932248880979682No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG26020.10869587920637805No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA25710.10740088602597925No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC24510.10238800919862898No Hit
TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT24140.10084237217686265No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA24120.10075882422974014No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG8750.052.0260779
TAGGCAT13250.034.714735
GGTATCA131600.033.0020331
TTAGGCA14550.032.62014
GGCATTC14350.031.7232197
CCTTTAG15300.031.643481
TGTCGCG504.6120073E-528.80260364-65
GCATTCC16850.028.4236188
GTATCAA186950.028.1820771
CTTTAGG18050.027.3432372
CCGGTAT458.416321E-426.66907564-65
GTACACG1109.528153E-525.8901211
GACATAT12600.025.6161521
GTACATG113150.025.5049531
CGTTCGA500.001574138823.95378562-63
TACATGG119150.023.499132
ATGGGTA14400.023.3804975
ACATGGG115550.022.9542453
CGCAATG852.8762588E-622.59027564-65
TCAACGC231800.022.4821174
Produced by FastQC (version 0.11.5)