##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939522_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2393835 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.927426075732036 31.0 31.0 31.0 30.0 34.0 2 31.186392545852158 31.0 31.0 34.0 30.0 34.0 3 31.171113297282393 31.0 31.0 34.0 28.0 34.0 4 35.09764624545969 35.0 35.0 37.0 33.0 37.0 5 34.70530884543003 35.0 35.0 37.0 32.0 37.0 6 34.76386718382846 35.0 35.0 37.0 32.0 37.0 7 34.61482432999768 35.0 35.0 37.0 30.0 37.0 8 34.517643446603465 35.0 35.0 37.0 30.0 37.0 9 35.98074345140747 37.0 35.0 39.0 30.0 39.0 10-11 35.810434511986 37.0 35.0 39.0 30.0 39.0 12-13 35.82326935649282 37.0 35.0 39.0 30.0 39.0 14-15 36.609464520319904 38.0 35.5 40.0 30.5 41.0 16-17 36.54212612815837 38.0 35.0 40.0 30.5 41.0 18-19 36.64816392942705 38.0 35.0 40.0 30.5 41.0 20-21 36.56983208951327 38.0 35.0 40.0 30.0 41.0 22-23 36.40864219965035 38.0 35.0 40.0 30.0 41.0 24-25 36.26995699369422 38.0 34.0 40.0 30.0 41.0 26-27 35.82546374332399 38.0 34.0 40.0 28.0 41.0 28-29 35.637436790756254 38.0 34.0 40.0 27.0 41.0 30-31 35.31164741930834 38.0 34.0 40.0 27.0 41.0 32-33 35.005170991317286 37.0 33.5 40.0 27.0 41.0 34-35 35.16371554430443 38.0 33.5 40.0 27.0 41.0 36-37 34.931326302773584 38.0 33.0 40.0 25.5 41.0 38-39 34.728075034411305 38.0 33.0 40.0 25.0 41.0 40-41 34.60537526604799 38.0 33.0 40.0 25.0 41.0 42-43 34.43283977383571 37.5 33.0 40.0 24.5 41.0 44-45 33.99929443758655 37.0 33.0 40.0 24.0 41.0 46-47 33.524969766086635 37.0 33.0 40.0 22.0 41.0 48-49 33.11558252761782 37.0 31.5 40.0 19.0 41.0 50-51 32.92550447294822 37.0 31.0 40.0 18.0 41.0 52-53 32.87859794012536 37.0 31.0 40.0 18.0 41.0 54-55 32.57156633602567 37.0 31.0 39.5 15.5 40.5 56-57 32.13780461059346 36.0 30.5 39.0 13.5 40.0 58-59 31.767743599705074 36.0 30.0 39.0 9.0 40.0 60-61 31.454361725014465 36.0 29.0 39.0 8.0 40.0 62-63 31.34644221510672 35.5 29.0 39.0 8.0 40.0 64-65 30.959806544728437 35.0 29.0 38.5 7.5 40.0 66-67 30.14428897563951 34.5 27.5 38.0 4.5 40.0 68-69 26.741260780296052 32.0 19.5 37.0 2.0 39.0 70-71 25.49746181336642 30.5 13.5 36.0 2.0 39.0 72-73 26.513500512775526 31.0 20.0 36.0 2.0 39.0 74-75 27.231597206992127 31.5 23.0 36.0 2.0 38.0 76-77 26.83979472269392 31.5 24.0 34.5 2.0 37.5 78-79 27.908182268201443 32.5 26.0 35.0 2.0 37.5 80-81 27.887669993963662 33.0 26.0 35.0 2.0 37.0 82-83 27.537588221410417 33.0 26.0 35.0 2.0 37.0 84-85 27.14127435683746 32.5 26.0 35.0 2.0 36.0 86-87 26.708087441281457 32.0 25.0 35.0 2.0 36.0 88-89 26.202917703183385 32.0 24.5 34.5 2.0 35.5 90-91 25.607994494190283 31.5 22.0 34.0 2.0 35.0 92-93 25.069155768881316 31.0 20.0 34.0 2.0 35.0 94-95 24.224963082250866 31.0 16.0 34.0 2.0 35.0 96-97 23.577877547951303 31.0 4.5 34.0 2.0 35.0 98-99 22.77556765608323 30.5 2.0 34.0 2.0 35.0 100-101 21.35626724481846 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.35297999402441604 1101 2 0.3676520035216164 1101 3 0.3999184753646716 1101 4 0.4466590216379345 1101 5 0.5919105491926686 1101 6 0.46943918940522167 1101 7 0.6806837333027076 1101 8 0.7604081262946636 1101 9 0.8056545063903826 1101 10-11 0.8625805014096883 1101 12-13 0.7780520447786756 1101 14-15 0.8758413211137608 1101 16-17 0.855857291675882 1101 18-19 0.6847905174255686 1101 20-21 0.8115101704700933 1101 22-23 0.8199723534555332 1101 24-25 0.9551333553973649 1101 26-27 1.0348227139780022 1101 28-29 1.0011621550559084 1101 30-31 0.8185511851680474 1101 32-33 1.1359285401188828 1101 34-35 1.0618832740131552 1101 36-37 1.2725651022336493 1101 38-39 1.2834408951013998 1101 40-41 1.3349033275465843 1101 42-43 1.0411862029745365 1101 44-45 1.199085615456248 1101 46-47 1.1820598318307063 1101 48-49 1.2101086074449725 1101 50-51 0.8490512937374319 1101 52-53 0.6731500007791595 1101 54-55 0.6422232528219141 1101 56-57 0.7388694891368033 1101 58-59 0.7558106782643677 1101 60-61 0.6180750197712293 1101 62-63 0.32319817384794547 1101 64-65 0.3982405782369547 1101 66-67 0.14649383205447108 1101 68-69 -6.729744555851749 1101 70-71 -7.672958397440528 1101 72-73 -4.421896324164582 1101 74-75 -2.3729502197898533 1101 76-77 0.1743722271776953 1101 78-79 0.28672451908970586 1101 80-81 0.19896716409598625 1101 82-83 -0.1316160981773251 1101 84-85 -0.02827158238635974 1101 86-87 -0.18380383759896546 1101 88-89 0.05593119068559815 1101 90-91 -0.1882224775727508 1101 92-93 -0.24852361495613806 1101 94-95 -0.1302002119064518 1101 96-97 -0.19503081030781289 1101 98-99 -0.15481424502490881 1101 100-101 -0.08555227806064636 1102 1 0.353259881643325 1102 2 0.41482342255776317 1102 3 0.398400691553892 1102 4 0.3580612763605231 1102 5 0.41104634143439966 1102 6 0.3849687509816846 1102 7 0.5371439892608265 1102 8 0.6500667225724541 1102 9 0.6516293318896231 1102 10-11 0.6868001297567972 1102 12-13 0.6556408573214298 1102 14-15 0.8521943676875381 1102 16-17 0.759223872265764 1102 18-19 0.7488001649683511 1102 20-21 0.6647416594248909 1102 22-23 0.6712934466859224 1102 24-25 0.6620161647353129 1102 26-27 0.6625961458380942 1102 28-29 0.5473208306253241 1102 30-31 0.655649786084993 1102 32-33 0.9538761413695624 1102 34-35 0.7407276162594982 1102 36-37 0.7997207102821093 1102 38-39 0.9855789288109875 1102 40-41 0.8311283950628194 1102 42-43 0.6805657992262866 1102 44-45 0.5683023561678837 1102 46-47 0.8891698649438382 1102 48-49 1.1028225942517977 1102 50-51 0.9870740563524905 1102 52-53 0.7427559213786523 1102 54-55 0.5194984492166341 1102 56-57 0.7662549837390529 1102 58-59 0.816462857837422 1102 60-61 0.9544381020617401 1102 62-63 0.7756613624129152 1102 64-65 0.7168423354341513 1102 66-67 0.561956824165545 1102 68-69 -6.583377828513175 1102 70-71 -7.057666000679415 1102 72-73 -0.7181742330431113 1102 74-75 0.7632920315243794 1102 76-77 1.2402641633372795 1102 78-79 0.9696440960747807 1102 80-81 0.7213969318306823 1102 82-83 0.5782948821749194 1102 84-85 0.32179060828752526 1102 86-87 0.31117830403245605 1102 88-89 0.34063755129446704 1102 90-91 0.4350683144204517 1102 92-93 0.5156728412997325 1102 94-95 0.6242631967815413 1102 96-97 0.4382714375507213 1102 98-99 0.30226294715196644 1102 100-101 0.2576378727354651 1103 1 0.358364818898238 1103 2 0.3004831658303182 1103 3 0.4005415470550524 1103 4 0.2790041055020893 1103 5 0.3664753126906888 1103 6 0.3479407992527044 1103 7 0.49289010266338096 1103 8 0.48413684194171935 1103 9 0.3889772292313509 1103 10-11 0.3939544010132039 1103 12-13 0.3786417228917145 1103 14-15 0.5085479280315681 1103 16-17 0.6043663701310393 1103 18-19 0.6542249310249204 1103 20-21 0.6121836714727849 1103 22-23 0.5411159405328974 1103 24-25 0.6603813933404865 1103 26-27 0.6829650820276925 1103 28-29 0.4577833675522385 1103 30-31 0.47822656894366133 1103 32-33 0.6267162748612947 1103 34-35 0.590992865072991 1103 36-37 0.7905481183722145 1103 38-39 0.8638419009760696 1103 40-41 0.7579225844112756 1103 42-43 0.40717627391156697 1103 44-45 0.6761292257588067 1103 46-47 0.9246232029963366 1103 48-49 0.8887436147603225 1103 50-51 0.6213390750481054 1103 52-53 0.47938192610016017 1103 54-55 0.3684827182653123 1103 56-57 0.6077204140769936 1103 58-59 0.7779375337742209 1103 60-61 0.6546999341504502 1103 62-63 0.251200825292095 1103 64-65 0.35557888317543274 1103 66-67 0.1648233102422445 1103 68-69 -7.07509898755967 1103 70-71 -8.036534433997211 1103 72-73 -0.9989025619551448 1103 74-75 0.2570459958012066 1103 76-77 0.6208782509899962 1103 78-79 0.4288825694471079 1103 80-81 0.2669328202900978 1103 82-83 0.15944311780160447 1103 84-85 -0.05211910607428294 1103 86-87 0.020753339397014514 1103 88-89 0.22382469553164341 1103 90-91 0.03255696330145952 1103 92-93 -0.030945448048381508 1103 94-95 0.21674210945706562 1103 96-97 0.2550121916598478 1103 98-99 0.3558663944812537 1103 100-101 0.3811085233616289 1104 1 0.3413025537025298 1104 2 0.32593683644390126 1104 3 0.19155633351650536 1104 4 0.243729363425075 1104 5 0.44721041155361263 1104 6 0.39892761436276203 1104 7 0.3567647308854234 1104 8 0.5466495296879685 1104 9 0.5176190983188604 1104 10-11 0.5340770067545009 1104 12-13 0.48241344971233246 1104 14-15 0.5885282409686212 1104 16-17 0.5116268276414573 1104 18-19 0.520652873696605 1104 20-21 0.4810172108335209 1104 22-23 0.5202160370245608 1104 24-25 0.5015746504998333 1104 26-27 0.6278121509531402 1104 28-29 0.6840936716003085 1104 30-31 0.6278832331310866 1104 32-33 0.7739558963928417 1104 34-35 0.5565687958892482 1104 36-37 0.887588968811869 1104 38-39 0.85499395265483 1104 40-41 0.7985693722410616 1104 42-43 0.8307314689551575 1104 44-45 0.8785407829890701 1104 46-47 0.9185760611622342 1104 48-49 0.9497966177932895 1104 50-51 0.7942498405630047 1104 52-53 0.6502317697007385 1104 54-55 0.6645667722626243 1104 56-57 0.6797101463968644 1104 58-59 0.7911515785370256 1104 60-61 0.976416259036224 1104 62-63 0.4942904847896976 1104 64-65 0.5971514121639636 1104 66-67 0.7089552869909532 1104 68-69 -6.435145911704442 1104 70-71 -3.570706475949052 1104 72-73 0.7878439586868993 1104 74-75 1.34449426219075 1104 76-77 1.4976025966910207 1104 78-79 0.9434100928940587 1104 80-81 0.6335380674668301 1104 82-83 0.48044720120426376 1104 84-85 0.6159590858603252 1104 86-87 0.5195668461561844 1104 88-89 0.5155910284943879 1104 90-91 0.4143345953771167 1104 92-93 0.6607416701854341 1104 94-95 0.7375040134785316 1104 96-97 0.6088938429543624 1104 98-99 0.7767168859667528 1104 100-101 0.8649191751079393 1105 1 0.06714756421057899 1105 2 0.16764392951760243 1105 3 0.06047927261489505 1105 4 0.13598749518980924 1105 5 0.2633285749833476 1105 6 0.051239807643241875 1105 7 0.1602674607243344 1105 8 0.3283266480598712 1105 9 0.35066592533891594 1105 10-11 0.21664259050054824 1105 12-13 0.23262452626613594 1105 14-15 0.313582964519604 1105 16-17 0.2755494991085854 1105 18-19 0.36983054141689564 1105 20-21 0.40642527808503104 1105 22-23 0.3146841691836926 1105 24-25 0.33636417963782606 1105 26-27 0.5294506177358755 1105 28-29 0.38701592330892254 1105 30-31 0.4614016546137094 1105 32-33 0.5022233220054346 1105 34-35 0.37038779545540024 1105 36-37 0.5571671115072263 1105 38-39 0.5469138125850748 1105 40-41 0.44831857357302596 1105 42-43 0.4273098736474452 1105 44-45 0.5638284584284108 1105 46-47 0.44585599783223273 1105 48-49 0.63978432084091 1105 50-51 0.37942165983584175 1105 52-53 0.36258689667064914 1105 54-55 0.627837972884663 1105 56-57 0.5686400720798872 1105 58-59 0.666988560107086 1105 60-61 0.6798815204978901 1105 62-63 0.4992353950064512 1105 64-65 0.33759500394458186 1105 66-67 0.5098554436256713 1105 68-69 -2.736359760354734 1105 70-71 -2.7124916046656082 1105 72-73 1.0168355381621161 1105 74-75 1.3567381813266692 1105 76-77 1.393434662297306 1105 78-79 1.0220837545968742 1105 80-81 0.6834815127545504 1105 82-83 0.6730861440889342 1105 84-85 0.6266933533676387 1105 86-87 0.5393645169190187 1105 88-89 0.4699560240150866 1105 90-91 0.3824129465431447 1105 92-93 0.4266857324336506 1105 94-95 0.4645848465724072 1105 96-97 0.3979583589925184 1105 98-99 0.27966146375730006 1105 100-101 0.5075753027370631 1106 1 0.08454184518484809 1106 2 0.2588659692996593 1106 3 0.2468666164439064 1106 4 0.1443453064380975 1106 5 0.2898823307572016 1106 6 0.29451160056292025 1106 7 0.3785788822993652 1106 8 0.4020262884191723 1106 9 0.30198688051734024 1106 10-11 0.3293535172145283 1106 12-13 0.27880888331606 1106 14-15 0.4115543040533822 1106 16-17 0.4313576538052146 1106 18-19 0.49024881548242405 1106 20-21 0.45548754194653895 1106 22-23 0.41358955186299085 1106 24-25 0.46609703420776327 1106 26-27 0.6204800537306312 1106 28-29 0.5833311685520641 1106 30-31 0.542286438914509 1106 32-33 0.6526885681008068 1106 34-35 0.43775587038339125 1106 36-37 0.5529274845563279 1106 38-39 0.7199558871380773 1106 40-41 0.6917418502236501 1106 42-43 0.6141436435114898 1106 44-45 0.6184862458142746 1106 46-47 0.7023362062306617 1106 48-49 0.8338950623370849 1106 50-51 0.5741750531282293 1106 52-53 0.5387780289422892 1106 54-55 0.40065761563020175 1106 56-57 0.667181339855972 1106 58-59 0.898107281308846 1106 60-61 0.9858921506361149 1106 62-63 0.5655535236248745 1106 64-65 0.7060508783600987 1106 66-67 0.6905536607622942 1106 68-69 -2.926997668075362 1106 70-71 -2.7257815500661273 1106 72-73 1.1413215729671542 1106 74-75 1.7225361660888083 1106 76-77 1.4661702184336356 1106 78-79 1.082295377297985 1106 80-81 0.8788360826913149 1106 82-83 0.6991194009110302 1106 84-85 0.595644088841393 1106 86-87 0.7729250166661892 1106 88-89 0.7584430474059367 1106 90-91 0.8691843616412385 1106 92-93 0.8336782725878464 1106 94-95 1.266143502663855 1106 96-97 1.236326780493691 1106 98-99 1.3276997032791442 1106 100-101 1.1471925240155194 1107 1 3.324603628769296E-4 1107 2 0.027852266496250166 1107 3 0.03753683357912507 1107 4 -0.1258285710207545 1107 5 0.20611697763597192 1107 6 0.06678298857329423 1107 7 0.06722549003848854 1107 8 0.0676756241641101 1107 9 0.2331342985957221 1107 10-11 0.008609412311777476 1107 12-13 0.10434979499868291 1107 14-15 0.035241305289382296 1107 16-17 -0.07608571578996504 1107 18-19 -0.010315037838061869 1107 20-21 0.14112983268600487 1107 22-23 0.31381971945111076 1107 24-25 0.1346882101301503 1107 26-27 0.4254687684827303 1107 28-29 0.18574064670250579 1107 30-31 0.2868649011010831 1107 32-33 0.27439579784429213 1107 34-35 0.16593431549296156 1107 36-37 0.10617551322664553 1107 38-39 0.2254502773888376 1107 40-41 0.16769028185458978 1107 42-43 0.0886294481080867 1107 44-45 0.36522424911157003 1107 46-47 0.4799354540097269 1107 48-49 0.2925333978474285 1107 50-51 0.33069372856604673 1107 52-53 0.03642652035760108 1107 54-55 0.27563569295954693 1107 56-57 0.5540007831785587 1107 58-59 0.6125046782269266 1107 60-61 0.6007614409425592 1107 62-63 0.3332496000655638 1107 64-65 0.025765231353847895 1107 66-67 0.25947757038927577 1107 68-69 -2.2088947262054326 1107 70-71 -2.630236117739159 1107 72-73 0.9686445616262738 1107 74-75 1.0661441847211819 1107 76-77 0.7422017442381268 1107 78-79 0.6700726349314259 1107 80-81 0.5454320910471431 1107 82-83 0.5069253740819946 1107 84-85 0.2279155542040172 1107 86-87 0.17888817646419852 1107 88-89 0.24567918138182776 1107 90-91 0.3002653609074315 1107 92-93 0.27354143049667456 1107 94-95 0.6249109271892479 1107 96-97 0.5650906780537177 1107 98-99 0.6005803890604469 1107 100-101 0.8502630218204281 1108 1 0.04075007735033154 1108 2 -9.089381502427329E-4 1108 3 -0.00381550783153628 1108 4 0.004647961701472525 1108 5 -0.15904909984313775 1108 6 -0.09484639258868555 1108 7 0.13110774134673164 1108 8 0.23981174287954587 1108 9 0.19905280808790593 1108 10-11 0.16084487344223675 1108 12-13 0.12287291228348352 1108 14-15 0.12455710906552753 1108 16-17 0.19828105297435172 1108 18-19 0.13851437389732268 1108 20-21 0.15740405255439072 1108 22-23 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2312 16-17 -0.9911985484872687 2312 18-19 -0.2021891819248509 2312 20-21 0.29140616002548114 2312 22-23 0.2836573669928626 2312 24-25 0.5220042286509283 2312 26-27 0.5109968922977828 2312 28-29 0.20299168249518829 2312 30-31 0.20264130207373654 2312 32-33 0.5154374811798874 2312 34-35 0.4905626998651087 2312 36-37 0.6711260630403615 2312 38-39 0.7016890831166833 2312 40-41 0.53750450592905 2312 42-43 0.0049044674440352765 2312 44-45 0.6429309061305517 2312 46-47 0.5842438065630589 2312 48-49 0.5637968619927136 2312 50-51 0.7489287239219422 2312 52-53 0.5053327372009306 2312 54-55 0.5619472853726606 2312 56-57 0.4010130735414208 2312 58-59 0.6410378241725141 2312 60-61 0.6054742541589597 2312 62-63 0.6582015065850548 2312 64-65 0.6833277644742815 2312 66-67 1.213668167838435 2312 68-69 3.8435657235019463 2312 70-71 4.51651248467995 2312 72-73 2.8372713782495147 2312 74-75 2.227065144433979 2312 76-77 2.0635885294600094 2312 78-79 1.1294990150896602 2312 80-81 1.0530928403893718 2312 82-83 1.1480427778341529 2312 84-85 0.9744043532642976 2312 86-87 0.9623460484306001 2312 88-89 0.9483107267624753 2312 90-91 1.0469382429099774 2312 92-93 1.4552159301099579 2312 94-95 1.4636287590574568 2312 96-97 1.5056991432020332 2312 98-99 1.751893958553353 2312 100-101 1.8319886588053897 2313 1 -0.29295042780278635 2313 2 -0.13767765987886804 2313 3 -0.20098805866041758 2313 4 -0.0683570062121035 2313 5 -0.043124453989435096 2313 6 0.05517718904741997 2313 7 -2.6174584731735386 2313 8 -2.8751540509120623 2313 9 -0.848863939568048 2313 10-11 -0.006363229545335969 2313 12-13 0.09435206032261334 2313 14-15 -0.5457351271850612 2313 16-17 -0.25247770358761557 2313 18-19 0.08879242082441152 2313 20-21 -2.4872625918631357 2313 22-23 -0.5844460100907085 2313 24-25 0.08484969324625524 2313 26-27 -1.6257010433137111 2313 28-29 -1.5284057822052546 2313 30-31 -0.779186439073456 2313 32-33 -0.9442814643096682 2313 34-35 -1.156726042376313 2313 36-37 -1.9912437044320583 2313 38-39 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60-61 -0.6494999729034099 2314 62-63 -0.8911235015383205 2314 64-65 -0.994953901312055 2314 66-67 -0.8875552057324576 2314 68-69 -1.057069975559461 2314 70-71 1.6174012609628328 2314 72-73 1.0967585533322861 2314 74-75 0.8915245425739755 2314 76-77 1.1003749068055875 2314 78-79 -1.423744538813068 2314 80-81 -0.616468897999205 2314 82-83 0.31216400010551837 2314 84-85 0.41305424068215046 2314 86-87 0.23095180479224453 2314 88-89 0.3123067702837439 2314 90-91 0.7868477191346024 2314 92-93 0.1319376926038558 2314 94-95 0.6832326531257458 2314 96-97 0.961334405676606 2314 98-99 0.6442069526658685 2314 100-101 0.924768371402422 2315 1 -0.3642695261617348 2315 2 -0.3914656176516438 2315 3 -0.4264807822556058 2315 4 -0.21158806357978222 2315 5 -0.21493793462644817 2315 6 -0.3086752772718384 2315 7 -0.9743169846810034 2315 8 -2.831373353431175 2315 9 -0.8917759611328222 2315 10-11 -1.34774954721248 2315 12-13 -0.7028528694724514 2315 14-15 -0.457246765451913 2315 16-17 -0.2918657349111342 2315 18-19 -0.14927572545056478 2315 20-21 -0.6740546897083632 2315 22-23 -0.37173817712670854 2315 24-25 -4.695392123760264 2315 26-27 -1.0293860679664846 2315 28-29 -0.7737076814445345 2315 30-31 -1.0187137523673755 2315 32-33 -0.22389440355259183 2315 34-35 -2.4231706982896597 2315 36-37 -3.629808412968302 2315 38-39 -1.1538497894845037 2315 40-41 -0.3371114814465841 2315 42-43 -1.7695004056255215 2315 44-45 -5.040611318212861 2315 46-47 -3.2659139486392093 2315 48-49 -2.5444200941470925 2315 50-51 -1.1715751045937566 2315 52-53 -0.23273680947396258 2315 54-55 -1.240862170255781 2315 56-57 -1.0824782126204866 2315 58-59 -0.6146972913548829 2315 60-61 -1.8728735602422653 2315 62-63 -0.787142219915939 2315 64-65 -0.2860552264220644 2315 66-67 -0.0437732038462606 2315 68-69 2.7322393644011065 2315 70-71 3.327036777400089 2315 72-73 -2.407609606245714 2315 74-75 -1.7839090445817547 2315 76-77 -0.5785062699048247 2315 78-79 -0.4259702436436932 2315 80-81 -0.6837484587664662 2315 82-83 -1.2011811930515393 2315 84-85 -0.7771766572187886 2315 86-87 -0.3803053654374331 2315 88-89 -0.5353455773686058 2315 90-91 -0.3052061507774475 2315 92-93 -1.414785482918159 2315 94-95 -0.4295641014944529 2315 96-97 -0.6202448929793896 2315 98-99 -2.9956604158556246 2315 100-101 -4.2419339566602545 2316 1 -0.6511133854693618 2316 2 -0.4899369262678803 2316 3 -1.0361338774159492 2316 4 -0.4598861959358871 2316 5 -0.3668354198572672 2316 6 -0.5036850447453745 2316 7 -0.9473308200136827 2316 8 -1.1701135182582973 2316 9 -5.354828743026186 2316 10-11 -1.6797170965713377 2316 12-13 -0.8873271445264379 2316 14-15 -0.6858258889585684 2316 16-17 -0.5666085712481674 2316 18-19 -0.6702164058115372 2316 20-21 -3.8788183908488847 2316 22-23 -1.2020967611084856 2316 24-25 -0.6487196604211363 2316 26-27 -0.858585049339375 2316 28-29 -0.2589511014705579 2316 30-31 -0.2518353007063112 2316 32-33 -1.4328085436261517 2316 34-35 -0.653003801311165 2316 36-37 -0.5574262862383463 2316 38-39 -5.563821460325734 2316 40-41 -5.2243852382855565 2316 42-43 -3.835259525732468 2316 44-45 -2.534827917501925 2316 46-47 -2.7886363952747004 2316 48-49 -3.060355178922755 2316 50-51 -1.470285921420519 2316 52-53 -1.0624295268478292 2316 54-55 -0.3523763940888429 2316 56-57 -3.6891903410717433 2316 58-59 -1.0351186574284235 2316 60-61 -0.7786701254591222 2316 62-63 -0.6448426247177927 2316 64-65 -0.4780934619385597 2316 66-67 -0.5270785458472567 2316 68-69 2.3955199914498486 2316 70-71 3.136841840828339 2316 72-73 1.8657328591257354 2316 74-75 0.2647655765566377 2316 76-77 -2.147477824383625 2316 78-79 -0.48906951688789135 2316 80-81 -0.45906090799196164 2316 82-83 -0.3405851331801131 2316 84-85 -0.06500155373933936 2316 86-87 0.0178717392614125 2316 88-89 -0.29263150132119264 2316 90-91 -0.4595539500527792 2316 92-93 -0.4116415522379988 2316 94-95 -0.1322210795137515 2316 96-97 -0.15704250224076688 2316 98-99 -0.19379883138534382 2316 100-101 -0.06553949765710243 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 22.0 4 58.0 5 169.0 6 471.0 7 1119.0 8 2301.0 9 4042.0 10 7015.0 11 10309.0 12 13772.0 13 16854.0 14 20982.0 15 26783.0 16 32377.0 17 36542.0 18 31103.0 19 24893.0 20 24120.0 21 24241.0 22 25751.0 23 28921.0 24 34582.0 25 42836.0 26 52357.0 27 66551.0 28 81908.0 29 97782.0 30 116024.0 31 135874.0 32 157727.0 33 183742.0 34 214405.0 35 246335.0 36 264539.0 37 243160.0 38 116745.0 39 7420.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.113783278660165 25.290883390513486 16.733707626206854 20.861625704619495 2 14.010356779955613 23.863461641308128 41.24800284782202 20.878178730914247 3 15.87912551559807 29.44475403768534 34.35326436134349 20.322856085373097 4 10.626491988577989 21.35003510315393 42.02497738888566 25.99849551938242 5 9.862166774234648 41.217794877257624 37.43336529042311 11.486673058084621 6 24.612306194871408 43.43001919514085 19.23140066044652 12.726273949541216 7 23.17770439483089 37.23706938865878 24.00578987273559 15.579436343774738 8 23.526558848040903 37.610403390375694 21.440700800180462 17.422336961402937 9 24.811651596705705 17.604722129971364 24.368304415300134 33.2153218580228 10-11 21.463247926094773 29.81219960961784 30.337464989271172 18.38708747501621 12-13 22.566446726695865 28.790016855798335 30.192264713315666 18.451271704190138 14-15 20.62420759993901 29.62593495374577 29.12656887379456 20.62328857252066 16-17 17.47897660706949 32.70488213093583 30.47478686276316 19.341354399231523 18-19 17.186895908548323 30.758939900765824 34.377760348729495 17.67640384195636 20-21 18.130844439988554 29.925412570206383 34.359615428799394 17.584127561005666 22-23 18.033693244265578 30.121900734094652 33.72357219572126 18.120833825918517 24-25 17.645052377936597 30.851954000324177 34.13681260746709 17.36618101427213 26-27 17.75631536435264 30.31015619663828 34.58439921871393 17.34912922029514 28-29 17.178103376539177 30.242704940905966 34.85380222005738 17.725389462497475 30-31 17.978200229130483 29.92180948332851 34.60739212121149 17.492598166329515 32-33 17.925645760581027 30.139970578461373 34.30266086757822 17.63172279337938 34-35 18.063151034867374 30.34052765808124 34.065924884649206 17.530396422402177 36-37 18.488180416546896 29.530237541484833 33.94845689497478 18.03312514699349 38-39 17.585558883228778 30.95518764369234 33.585306310879766 17.87394716219912 40-41 17.591911272844033 30.314540242956145 34.49797898884221 17.59556949535761 42-43 17.538258213359622 30.313984515259385 34.07579478663118 18.07196248474981 44-45 18.138404464946085 30.262414671540565 33.57134226417319 18.02783859934016 46-47 18.45861184131447 30.658520907043226 32.50634710761078 18.376520144031524 48-49 17.922827451345174 30.66126249097243 32.77973977598033 18.636170281702064 50-51 17.893762974337385 30.730442522235222 32.51162268604502 18.864171817382367 52-53 17.989925761952552 31.63244705507643 32.22995445233922 18.147672730631793 54-55 18.535831436741027 31.017502006650734 31.774251217834816 18.672415338773423 56-57 18.47459032953524 31.444063708711468 31.62580982950045 18.455536132252842 58-59 18.16082007630084 30.866324362347303 32.04267599051431 18.930179570837545 60-61 18.51987248852104 31.3919515690346 31.737406570267098 18.35076937217726 62-63 18.307958991381657 30.784828824756733 32.28631840853174 18.62089377532987 64-65 18.28205178551361 30.894028903600773 31.930654084650378 18.893265226235243 66-67 18.115683580132586 31.07258678067496 31.408787962525032 19.402941676667425 68-69 18.783299732087563 30.178855111877002 31.538422379439403 19.49942277659603 70-71 18.461839913784658 30.69868770219889 32.20802506397149 18.631447320044963 72-73 19.127289862162844 30.145773095867234 31.679698291781794 19.04723875018813 74-75 18.238840722905618 31.15072361823117 31.27092785676804 19.339507802095174 76-77 17.666839803068154 31.205590199112443 31.976772656558346 19.150797341261054 78-79 18.12131303908045 30.881741293189368 31.299771818128836 19.697173849601345 80-81 17.95053624998068 30.82028513384631 31.25928197270144 19.969896643471568 82-83 18.067782774958914 31.443724898633718 31.110864176282504 19.377628150124863 84-85 17.886175805152575 31.044952193804743 30.946616136804924 20.122255864237758 86-87 17.54606675671944 31.011894778199263 31.544691944523755 19.897346520557534 88-89 18.409838476146554 30.540022541320884 31.268834911142246 19.78130407139032 90-91 17.922544941767818 30.659765600766836 31.72003267694669 19.697656780518656 92-93 18.199145603358097 30.84068167918267 31.48634602980446 19.47382668765478 94-95 17.996957416370755 30.356857685847178 31.39751493073294 20.24866996704913 96-97 18.37073399859003 30.07125899669907 30.928293440636935 20.629713564073963 98-99 17.84336978667162 30.30737410834644 30.783075863483706 21.06618024149823 100-101 17.689065606694072 30.303431638995438 31.330871966446082 20.676630787864404 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6419.0 1 4164.5 2 1918.5 3 1958.5 4 1743.0 5 1696.5 6 2801.5 7 5138.5 8 7440.0 9 8215.5 10 7174.0 11 5781.0 12 5153.0 13 4747.5 14 4531.0 15 4534.0 16 4663.0 17 5107.5 18 5581.0 19 6282.5 20 7398.5 21 8651.5 22 9952.0 23 11530.0 24 13529.0 25 18099.0 26 27636.5 27 36756.0 28 40015.0 29 44001.0 30 52870.0 31 63823.0 32 78023.5 33 94642.5 34 112695.0 35 123792.5 36 132937.5 37 143834.5 38 140390.5 39 129004.5 40 117877.5 41 107249.5 42 99752.0 43 93891.0 44 87147.0 45 79997.5 46 71263.5 47 61965.0 48 58600.0 49 51901.5 50 39628.0 51 29991.0 52 23073.5 53 18520.0 54 15246.5 55 12553.5 56 10191.0 57 8285.0 58 6680.5 59 5311.5 60 3946.5 61 2820.0 62 1959.0 63 1280.5 64 836.5 65 579.5 66 423.5 67 306.5 68 224.0 69 158.0 70 105.0 71 99.5 72 98.5 73 85.5 74 81.0 75 71.5 76 52.5 77 34.5 78 32.5 79 31.5 80 20.5 81 13.5 82 10.5 83 8.0 84 4.0 85 2.0 86 1.0 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021806014198973614 2 0.01783748671065466 3 5.430616562962777E-4 4 0.09658142687361493 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011822034517834354 12-13 0.0 14-15 0.0 16-17 5.639486430769038E-4 18-19 1.0443493390313034E-4 20-21 0.0 22-23 8.354794712250426E-5 24-25 0.003300143911338918 26-27 0.0037387706337320657 28-29 9.608013919087991E-4 30-31 1.0443493390313034E-4 32-33 4.386267223931474E-4 34-35 0.0020886986780626064 36-37 0.08970960822278895 38-39 0.10197026946301645 40-41 0.08200231010073794 42-43 0.08609615950974064 44-45 0.08555309785344437 46-47 0.08344351218860113 48-49 0.07759515589002584 50-51 0.07742805999578083 52-53 0.05831646709150797 54-55 0.048729340159200614 56-57 0.027633483510768286 58-59 0.02278770257766304 60-61 0.013576541407406943 62-63 0.001900715797036972 64-65 0.0064331919284328284 66-67 0.15036541783372706 68-69 0.7076093381540499 70-71 0.8650345575196285 72-73 0.3544103916936631 74-75 0.05182061420273327 76-77 0.19140834685765729 78-79 0.014600003759657622 80-81 0.003425465832022675 82-83 0.0017127329160113375 84-85 1.253219206837564E-4 86-87 0.0022349075855269893 88-89 3.7596576205126917E-4 90-91 0.004114736395783335 92-93 0.004135623382563961 94-95 0.07473363870108007 96-97 0.036510452892534365 98-99 0.013305010579258805 100-101 0.013117027698233169 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2393835.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.61986524747187 #Duplication Level Percentage of deduplicated Percentage of total 1 67.00802983990926 16.497286651571404 2 13.266744067646663 6.532509024363153 3 6.0423216291524104 4.462834328748512 4 3.3442284016654096 3.2933781042308254 5 2.1583845173451195 2.656956798463323 6 1.435359957351526 2.120302123896693 7 1.0259494014738093 1.7681115210506724 8 0.7571949273601013 1.4913629662139958 9 0.6102371200282931 1.3521560097691705 >10 3.5465916391278918 16.246151757714895 >50 0.37751438109064844 6.56232802134583 >100 0.3620335640160985 18.473523846344257 >500 0.0442483158280262 7.464457815695849 >1k 0.020462659888850064 8.571241142941362 >5k 1.7489452896453044E-4 0.38886399892445683 >10k+ 5.246835868935913E-4 2.118535888725745 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19857 0.8295057930057836 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16779 0.7009255023842496 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12565 0.524889977797133 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9031 0.377260755231668 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 4374 0.18271936035691683 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 3955 0.16521606543475217 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3801 0.15878287350631937 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3614 0.15097114045036522 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 3524 0.1472114828298525 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 3255 0.1359742839418757 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 3237 0.13522235241777317 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 2761 0.11533794100261714 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2717 0.11349988616592205 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 2677 0.11182892722347196 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 2618 0.10936426278335809 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 2617 0.10932248880979682 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 2602 0.10869587920637805 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 2571 0.10740088602597925 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 2451 0.10238800919862898 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 2414 0.10084237217686265 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 2412 0.10075882422974014 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.0886986780626066E-5 0.0 16-17 0.0 0.0 0.0 1.253219206837564E-4 0.0 18-19 0.0 0.0 0.0 2.2975685458688673E-4 0.0 20-21 0.0 0.0 0.0 6.26609603418782E-4 0.0 22-23 0.0 0.0 0.0 0.0014620890746438247 0.0 24-25 0.0 2.0886986780626066E-5 0.0 0.0024437774533332497 0.0 26-27 0.0 4.177397356125213E-5 0.0 0.00382231858085457 0.0 28-29 0.0 4.177397356125213E-5 0.0 0.007435767293902879 0.0 30-31 0.0 4.177397356125213E-5 0.0 0.017085555186552123 0.0 32-33 0.0 4.177397356125213E-5 0.0 0.03557053848740619 0.0 34-35 0.0 4.177397356125213E-5 0.0 0.06487498094062456 0.0 36-37 0.0 4.177397356125213E-5 0.0 0.10677427642256046 0.0 38-39 0.0 4.177397356125213E-5 0.0 0.16613509285309974 0.0 40-41 0.0 4.177397356125213E-5 0.0 0.23330764233959317 0.0 42-43 0.0 4.177397356125213E-5 0.0 0.30346703093571614 0.0 44-45 0.0 4.177397356125213E-5 0.0 0.3864301424283628 0.0 46-47 0.0 4.177397356125213E-5 0.0 0.48449454536340225 0.0 48-49 0.0 4.177397356125213E-5 0.0 0.5910181779445951 0.0 50-51 0.0 4.177397356125213E-5 0.0 0.7031812969565572 0.0 52-53 0.0 4.177397356125213E-5 0.0 0.8159292515983767 0.0 54-55 0.0 4.177397356125213E-5 0.0 0.9391833605908511 0.0 56-57 0.0 4.177397356125213E-5 0.0 1.0847029974914728 0.0 58-59 0.0 4.177397356125213E-5 0.0 1.2285099014760834 0.0 60-61 0.0 4.177397356125213E-5 0.0 1.3768910555656508 0.0 62-63 0.0 8.354794712250426E-5 0.0 1.5117165552345921 0.0 64-65 0.0 8.354794712250426E-5 0.0 1.6685987129438746 0.0 66-67 0.0 8.354794712250426E-5 0.0 1.8289272234719602 0.0 68-69 0.0 8.354794712250426E-5 0.0 2.012168758498393 0.0 70-71 0.0 8.354794712250426E-5 0.0 2.2497791201147948 0.0 72-73 0.0 8.354794712250426E-5 0.0 2.502532547147151 0.0 74-75 0.0 8.354794712250426E-5 0.0 2.780601002157626 0.0 76-77 0.0 8.354794712250426E-5 0.0 3.095931841584737 0.0 78-79 0.0 8.354794712250426E-5 0.0 3.4568798601407367 0.0 80-81 0.0 8.354794712250426E-5 0.0 3.8500564992992414 0.0 82-83 0.0 8.354794712250426E-5 0.0 4.25135817631541 0.0 84-85 0.0 8.354794712250426E-5 0.0 4.645955130574998 0.0 86-87 0.0 8.354794712250426E-5 0.0 5.080655099453388 0.0 88-89 0.0 8.354794712250426E-5 0.0 5.536262942099184 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 875 0.0 52.026077 9 TAGGCAT 1325 0.0 34.71473 5 GGTATCA 13160 0.0 33.002033 1 TTAGGCA 1455 0.0 32.6201 4 GGCATTC 1435 0.0 31.723219 7 CCTTTAG 1530 0.0 31.64348 1 TGTCGCG 50 4.6120073E-5 28.802603 64-65 GCATTCC 1685 0.0 28.423618 8 GTATCAA 18695 0.0 28.182077 1 CTTTAGG 1805 0.0 27.343237 2 CCGGTAT 45 8.416321E-4 26.669075 64-65 GTACACG 110 9.528153E-5 25.890121 1 GACATAT 1260 0.0 25.616152 1 GTACATG 11315 0.0 25.504953 1 CGTTCGA 50 0.0015741388 23.953785 62-63 TACATGG 11915 0.0 23.49913 2 ATGGGTA 1440 0.0 23.380497 5 ACATGGG 11555 0.0 22.954245 3 CGCAATG 85 2.8762588E-6 22.590275 64-65 TCAACGC 23180 0.0 22.482117 4 >>END_MODULE