FastQCFastQC Report
Fri 27 May 2016
SRR939521_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939521_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804590
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57910.7197454604208354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41750.5188978237363129No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31350.3896394436918182No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22260.27666264805677426No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10010.12441119079282617No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33350.042.9496151
GTATCAA56300.036.8282431
TACAGGG1201.1845259E-732.5418972
CATTCCG2450.030.0658369
CTTTAGG2650.029.4719092
GGATATG1952.8921932E-1028.2518671
TCAACGC74350.028.132984
ATCAACG74000.028.0321453
GATTGGC1953.6379788E-1027.7016839
CAACGCA75500.027.514865
TATCAAC75350.027.2082232
AACGCAG74800.027.1938786
CCTTTAG2600.026.967691
GTACATG38050.026.7196311
GTCCAAG1706.717528E-826.514451
ACGCAGA80100.024.742147
CCGGGAA2001.4408215E-824.5537669
CGCAGAG80600.024.5171628
ACATGGG39500.024.378063
TACATGG41250.024.3768422
Produced by FastQC (version 0.11.5)