FastQCFastQC Report
Fri 27 May 2016
SRR939521_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939521_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804590
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74640.9276774506270273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62120.772070246958078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47680.5925999577424527No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32850.4082824792751588No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16550.20569482593619112No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11330.14081706210616587No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.12639978125504914No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT9990.12416261698504828No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA9950.12366546936949253No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8960.11136106588448774No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC8240.10241240880448427No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC8170.10154240047726172No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36350.042.641981
GTATCAA60200.035.5906521
ATACGAC600.004003891731.5795353
CTTTAGG1859.094947E-1230.7355862
CCTTTAG1901.2732926E-1129.9379221
CATTCCG2002.3646862E-1128.4215839
ATCAACG74850.028.4152553
TCAACGC75200.028.2830034
AACGCAG75250.028.2012626
CAACGCA76250.027.8935325
CCCTATA1703.7343852E-927.8729572
GTCGGTC350.00778147527.5667164-65
TATCAAC77650.027.3991362
TAGGCAT2104.3655746E-1127.0681745
GTACATG40200.027.0024641
GAGTCGG458.2552794E-426.75609262-63
TAAGGCG950.001246087824.9312135
ACGCAGA85600.024.62547
CGCAGAG86450.024.3832768
ACATGGG41600.024.3678653
Produced by FastQC (version 0.11.5)