##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939521_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 804590 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.965638399681826 31.0 31.0 31.0 30.0 34.0 2 31.232090878584124 31.0 31.0 34.0 30.0 34.0 3 31.19515405361737 31.0 31.0 34.0 28.0 34.0 4 35.16626977715358 35.0 35.0 37.0 33.0 37.0 5 34.7996644253595 35.0 35.0 37.0 32.0 37.0 6 34.76140270199729 35.0 35.0 37.0 32.0 37.0 7 34.62352751090618 35.0 35.0 37.0 30.0 37.0 8 34.6414732969587 35.0 35.0 37.0 30.0 37.0 9 35.96207633701637 37.0 35.0 39.0 30.0 39.0 10-11 35.82331933034216 37.0 35.0 39.0 30.0 39.0 12-13 35.81937508544725 37.0 35.0 39.0 30.0 39.0 14-15 36.621571235038964 38.0 35.5 40.0 30.5 41.0 16-17 36.64728370971551 38.0 35.5 40.0 30.5 41.0 18-19 36.68864328415715 38.5 35.0 40.0 31.0 41.0 20-21 36.621054822953305 38.0 35.0 40.0 30.0 41.0 22-23 36.46345902882213 38.0 35.0 40.0 30.0 41.0 24-25 36.26095651201233 38.0 34.5 40.0 29.5 41.0 26-27 35.87507736859767 38.0 34.0 40.0 28.5 41.0 28-29 35.58362706471619 38.0 34.0 40.0 27.0 41.0 30-31 35.31251569122162 38.0 34.0 40.0 27.0 41.0 32-33 35.00803079829478 37.0 33.0 40.0 27.0 41.0 34-35 34.86976161771834 37.0 33.0 40.0 26.5 41.0 36-37 34.76207074410569 37.5 33.0 40.0 25.0 41.0 38-39 34.68209150001864 38.0 33.0 40.0 25.0 41.0 40-41 34.47598839160318 37.0 33.0 40.0 24.5 41.0 42-43 34.127065337625375 37.0 33.0 40.0 24.0 41.0 44-45 33.86805577996246 37.0 33.0 40.0 23.5 41.0 46-47 33.449552567146 37.0 32.5 40.0 21.5 41.0 48-49 33.08307460942841 37.0 32.0 40.0 19.0 41.0 50-51 32.822376614176164 37.0 31.0 40.0 18.0 41.0 52-53 32.48073490846269 37.0 31.0 40.0 15.0 41.0 54-55 32.250446189984956 36.0 31.0 39.0 13.5 41.0 56-57 31.86277047937459 36.0 30.5 39.0 9.0 40.0 58-59 31.459849737133197 35.5 30.0 39.0 8.0 40.0 60-61 31.185112293217664 35.0 29.0 39.0 7.5 40.0 62-63 30.80825575759082 35.0 29.0 39.0 4.0 40.0 64-65 30.394073378988057 35.0 28.0 38.0 2.0 40.0 66-67 28.756823351023503 33.5 25.0 37.5 2.0 39.5 68-69 25.19406965038094 30.0 13.5 36.0 2.0 39.0 70-71 24.65335388210144 29.5 11.0 35.0 2.0 38.0 72-73 26.36961806634435 31.0 21.0 35.0 2.0 38.0 74-75 26.90925253856001 32.0 22.5 35.0 2.0 38.0 76-77 26.87283026137536 31.5 25.0 34.5 2.0 37.0 78-79 27.654794988752037 33.0 26.0 35.0 2.0 37.0 80-81 27.468546713232826 33.0 26.0 35.0 2.0 37.0 82-83 27.06007842503635 33.0 25.5 35.0 2.0 36.0 84-85 26.71617096906499 32.5 25.0 35.0 2.0 36.0 86-87 26.282323295094393 32.0 24.5 35.0 2.0 35.5 88-89 25.794520811842055 32.0 22.5 34.5 2.0 35.0 90-91 25.3537391715035 32.0 20.5 34.0 2.0 35.0 92-93 24.821412769236506 31.5 19.0 34.0 2.0 35.0 94-95 23.50536111559925 31.0 6.5 34.0 2.0 35.0 96-97 22.735314880871 30.0 2.0 34.0 2.0 35.0 98-99 22.451050845772382 30.0 2.0 34.0 2.0 35.0 100-101 21.214713705116893 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.27613969813603134 1101 2 0.20891403559177846 1101 3 0.33672608144209093 1101 4 0.30338567265995664 1101 5 0.38582040545917096 1101 6 0.5466661704850893 1101 7 0.6347998007136795 1101 8 0.5705462702909117 1101 9 0.6582026415297406 1101 10-11 0.6491299905992918 1101 12-13 0.40719042974321695 1101 14-15 0.48118497299688556 1101 16-17 0.440032613299131 1101 18-19 0.7642729029259883 1101 20-21 0.5941314971870213 1101 22-23 0.7439371682643738 1101 24-25 0.6857720537604095 1101 26-27 0.6825216055050518 1101 28-29 0.5652634499776852 1101 30-31 0.5644659309864934 1101 32-33 0.5723858116280383 1101 34-35 0.5480782777677362 1101 36-37 0.6602217916602413 1101 38-39 0.6792508210757973 1101 40-41 1.0394872808276219 1101 42-43 0.724724791543423 1101 44-45 0.41907609150022296 1101 46-47 0.10506372786937845 1101 48-49 -0.0023216486179933327 1101 50-51 -0.21228007451832553 1101 52-53 -0.11081518929886869 1101 54-55 0.09488341951478674 1101 56-57 0.05282636773415206 1101 58-59 0.28463053380981407 1101 60-61 -0.5562721639924213 1101 62-63 -0.20332523422440474 1101 64-65 -0.3135235701122774 1101 66-67 -2.495446151950489 1101 68-69 -7.790465213294134 1101 70-71 -11.31417518178013 1101 72-73 -11.452256456622479 1101 74-75 -9.462096034238481 1101 76-77 -4.9083967484358375 1101 78-79 -2.141055543729987 1101 80-81 -1.522915987869446 1101 82-83 -1.1247223615148698 1101 84-85 -1.2603284702745832 1101 86-87 -1.173102661855392 1101 88-89 -1.1228796669000616 1101 90-91 -1.3038442430420503 1101 92-93 -1.396046264835661 1101 94-95 -4.814302902700209 1101 96-97 -2.0129403031821482 1101 98-99 -0.9875592630149193 1101 100-101 -0.8647887662247378 1102 1 0.4353858968999198 1102 2 0.397155798000302 1102 3 0.4774950497050874 1102 4 0.3261400047420153 1102 5 0.5816380977960307 1102 6 0.511709965275216 1102 7 0.5960783810799626 1102 8 0.45829889608243946 1102 9 0.7724331045032358 1102 10-11 0.9440376613231365 1102 12-13 0.5211213439357536 1102 14-15 0.6586145411157887 1102 16-17 0.7236427448730964 1102 18-19 0.6822647885666058 1102 20-21 0.7276089172757594 1102 22-23 0.7936736411722762 1102 24-25 0.7608677956176138 1102 26-27 0.7616593600267194 1102 28-29 0.6460638898214697 1102 30-31 0.952055511239017 1102 32-33 0.6636211659676974 1102 34-35 0.8986356660057027 1102 36-37 1.2619356656980827 1102 38-39 1.1344796535939068 1102 40-41 0.8451426859768318 1102 42-43 0.8691641803122394 1102 44-45 0.7213776878298077 1102 46-47 0.6123325255033194 1102 48-49 0.5868680529707504 1102 50-51 0.7217338791464059 1102 52-53 0.7470223718432081 1102 54-55 0.6896962963206121 1102 56-57 0.7042198383290774 1102 58-59 0.3970125153818742 1102 60-61 0.3419608773756444 1102 62-63 0.5229687027566001 1102 64-65 0.5312153674374898 1102 66-67 -2.208436710476253 1102 68-69 -7.63943006370933 1102 70-71 -11.07987684636133 1102 72-73 -9.208507167576862 1102 74-75 -7.361938676334564 1102 76-77 -3.668484717191763 1102 78-79 -0.7108011168540145 1102 80-81 0.11085150160842971 1102 82-83 0.29243344176879305 1102 84-85 0.4023890817672786 1102 86-87 0.3324408864705468 1102 88-89 0.02038238140691817 1102 90-91 -0.22169204192726966 1102 92-93 -0.30174717106551796 1102 94-95 -3.7244041426046586 1102 96-97 -0.7425425479021293 1102 98-99 -0.05028439857022349 1102 100-101 0.20839782612349822 1103 1 0.3094536314934295 1103 2 0.22122074184421692 1103 3 0.44358434128880475 1103 4 0.19052569873942105 1103 5 0.486311052951784 1103 6 0.2888196169276469 1103 7 0.5532732436200405 1103 8 0.4474534229883673 1103 9 0.6092213858356033 1103 10-11 0.771963762273856 1103 12-13 0.5257304219341066 1103 14-15 0.8680702209113846 1103 16-17 0.7364206570202967 1103 18-19 0.6885700221523479 1103 20-21 0.7855368167095378 1103 22-23 0.762685157665608 1103 24-25 0.7131149692084406 1103 26-27 0.7361711338891865 1103 28-29 0.7305101120767645 1103 30-31 0.8001916005926191 1103 32-33 1.0136425617145974 1103 34-35 0.8903595430390752 1103 36-37 0.8009968575342086 1103 38-39 0.734084607484526 1103 40-41 0.8219503165063884 1103 42-43 0.9382153874664709 1103 44-45 0.9866297545349241 1103 46-47 0.8838954072933731 1103 48-49 0.9889139825074267 1103 50-51 0.9735060051394484 1103 52-53 1.0569289127400907 1103 54-55 1.2741784882373608 1103 56-57 1.2874629374254987 1103 58-59 1.2331868318032662 1103 60-61 1.130956021471345 1103 62-63 1.056695221699588 1103 64-65 1.0231519616066826 1103 66-67 -2.361290406979137 1103 68-69 -7.324289316904739 1103 70-71 -11.097626633954278 1103 72-73 -8.475272440729235 1103 74-75 -6.689729580971548 1103 76-77 -3.342288260823583 1103 78-79 -0.5517370722516333 1103 80-81 0.2175362771391356 1103 82-83 0.33403979723587085 1103 84-85 0.44968670590912296 1103 86-87 0.7441552129386366 1103 88-89 0.4533640802912373 1103 90-91 0.465642296075913 1103 92-93 0.3052917195805094 1103 94-95 0.976643938910037 1103 96-97 1.4081312630835008 1103 98-99 1.0930236939834366 1103 100-101 0.7797907822715153 1104 1 0.3097476994171693 1104 2 0.06880426546846863 1104 3 0.2670400084658269 1104 4 0.15927235029393216 1104 5 0.3532387693893497 1104 6 0.4540191187980156 1104 7 0.5053127892077143 1104 8 0.22220810843093375 1104 9 0.6518600442191556 1104 10-11 0.5473208702646488 1104 12-13 0.49923573539623334 1104 14-15 0.6736163181419101 1104 16-17 0.7187914635759896 1104 18-19 0.8899249966624936 1104 20-21 0.7682010959097738 1104 22-23 0.6233695858761266 1104 24-25 0.9084314315355968 1104 26-27 0.9053423385675998 1104 28-29 0.9849145828616557 1104 30-31 0.9149757166387289 1104 32-33 0.8308442256390123 1104 34-35 0.9992337272089387 1104 36-37 1.1294951347962083 1104 38-39 1.184303789285174 1104 40-41 1.008197700254314 1104 42-43 1.2386789909057612 1104 44-45 1.0695902446640062 1104 46-47 0.732851049468664 1104 48-49 1.0511706621353056 1104 50-51 0.8427493576566505 1104 52-53 0.9577899245718626 1104 54-55 0.611819658699126 1104 56-57 0.7511235235135878 1104 58-59 0.9582476098093693 1104 60-61 0.5313134271017361 1104 62-63 0.8246256264180083 1104 64-65 0.7985358571613723 1104 66-67 -2.3080629222242344 1104 68-69 -6.79427950670356 1104 70-71 -8.272467872992213 1104 72-73 -7.43612092788668 1104 74-75 -6.180449773556276 1104 76-77 -2.717871336797881 1104 78-79 -0.17657069171583473 1104 80-81 0.6680024998068887 1104 82-83 0.7545110916823958 1104 84-85 0.7387507127095922 1104 86-87 0.8907236664747131 1104 88-89 0.6001218326122313 1104 90-91 0.5952601744532302 1104 92-93 0.8716409436936026 1104 94-95 1.933143316247996 1104 96-97 1.9527795840389963 1104 98-99 1.7821236556474567 1104 100-101 1.6628005615181785 1105 1 0.5541120276324882 1105 2 0.4229929977604243 1105 3 0.4914646864187837 1105 4 0.25706195026484835 1105 5 0.5487876732781487 1105 6 0.5486445989904567 1105 7 0.4345848356455022 1105 8 0.43849647838209904 1105 9 0.6575702442942912 1105 10-11 0.8045622328599293 1105 12-13 0.7813271325290074 1105 14-15 1.0973829658230443 1105 16-17 1.0137762549573637 1105 18-19 0.9722278602454253 1105 20-21 1.096226156804839 1105 22-23 1.1611293745308586 1105 24-25 1.1395945576035302 1105 26-27 1.2183332861069545 1105 28-29 1.3685522905410963 1105 30-31 1.1015836493572593 1105 32-33 1.3321942177620372 1105 34-35 1.1533243002890785 1105 36-37 1.0632612977405032 1105 38-39 1.0494087954049718 1105 40-41 1.128499691310914 1105 42-43 1.2256771367873185 1105 44-45 1.1614054629773634 1105 46-47 1.455475535950562 1105 48-49 1.4525594332062113 1105 50-51 1.3749201304483663 1105 52-53 1.5750089892721135 1105 54-55 1.3868872188973853 1105 56-57 1.3062114426594782 1105 58-59 1.5957468524080411 1105 60-61 1.2555296682098422 1105 62-63 1.2738603481184647 1105 64-65 1.3821196378664276 1105 66-67 1.8484392166771109 1105 68-69 -5.053614326563228 1105 70-71 -4.779520188216583 1105 72-73 -1.255014758398442 1105 74-75 1.3832622200452 1105 76-77 1.553760650986753 1105 78-79 1.3227215924853795 1105 80-81 1.1594075095622216 1105 82-83 1.5771792044806574 1105 84-85 1.615276118979665 1105 86-87 1.6051833666649706 1105 88-89 1.4346266133293994 1105 90-91 1.4225114741539073 1105 92-93 1.5750952996601093 1105 94-95 2.358687736320949 1105 96-97 2.5415845865232747 1105 98-99 2.5617283527475188 1105 100-101 2.319286946471948 1106 1 0.14740811701796375 1106 2 0.23416618211796703 1106 3 0.22330826183777575 1106 4 0.08387759272294915 1106 5 0.21694409785915525 1106 6 0.3754602414485575 1106 7 0.350785952963939 1106 8 0.1803959197228835 1106 9 0.31790543982501873 1106 10-11 0.40121027755266425 1106 12-13 0.4109360710764989 1106 14-15 0.7007349211303051 1106 16-17 0.3053963666892088 1106 18-19 0.44150160326218213 1106 20-21 0.3330976651847237 1106 22-23 0.30302881587164165 1106 24-25 0.3697621553689032 1106 26-27 0.3345344034253941 1106 28-29 0.5467645810438881 1106 30-31 0.4256059957259737 1106 32-33 0.35789254178438057 1106 34-35 0.5052796075516497 1106 36-37 0.7582333647796062 1106 38-39 0.9147719238965877 1106 40-41 0.8793376801377306 1106 42-43 1.049699483156033 1106 44-45 1.1675507143740091 1106 46-47 1.0767045862298943 1106 48-49 0.8855203270609522 1106 50-51 0.7888866108952968 1106 52-53 0.9526626205570281 1106 54-55 0.7159374982269995 1106 56-57 0.43973099573210206 1106 58-59 0.6974228300616758 1106 60-61 0.8315073218411264 1106 62-63 0.7185530855486313 1106 64-65 0.912287235631787 1106 66-67 1.5367073731016916 1106 68-69 -5.629591980194515 1106 70-71 -5.838738065311556 1106 72-73 -2.013115317057661 1106 74-75 0.34192143792229146 1106 76-77 0.8654924945621723 1106 78-79 0.37467689974794993 1106 80-81 0.34711700676892576 1106 82-83 0.3984082547599854 1106 84-85 0.25438226423105803 1106 86-87 0.5068593443186025 1106 88-89 0.5039003563461577 1106 90-91 0.5487684573941287 1106 92-93 0.8985589868109471 1106 94-95 1.7391346636952498 1106 96-97 1.8901879384785794 1106 98-99 1.5282087996748928 1106 100-101 1.339398678315522 1107 1 -0.08131946646204824 1107 2 -0.05913628465583898 1107 3 -0.2509895877068793 1107 4 -0.06616322256237339 1107 5 0.10225569705305304 1107 6 -0.010265887986918187 1107 7 0.006333005335051212 1107 8 -0.15054716846364613 1107 9 0.01458154502641662 1107 10-11 0.05436580929930557 1107 12-13 0.13910563307747026 1107 14-15 0.03375384921887559 1107 16-17 -0.020595317196402618 1107 18-19 0.08430216957426495 1107 20-21 -0.06482394395789726 1107 22-23 -0.037987994924002066 1107 24-25 0.008168701120283117 1107 26-27 0.12781136064586462 1107 28-29 -0.03460324120554503 1107 30-31 0.04966405546934283 1107 32-33 -0.061761931315793106 1107 34-35 0.09030039783760202 1107 36-37 -0.08174903362859709 1107 38-39 -0.0036772228088537418 1107 40-41 -0.0660948343389336 1107 42-43 0.014288396704159823 1107 44-45 0.1959868512072518 1107 46-47 0.019294060988194417 1107 48-49 0.37594978039123106 1107 50-51 0.4152367040051672 1107 52-53 0.2743132162050941 1107 54-55 0.24935692630281636 1107 56-57 0.27653235284033784 1107 58-59 0.06421513127848044 1107 60-61 0.01036309082661191 1107 62-63 0.2036842046527383 1107 64-65 0.18612017356704413 1107 66-67 0.8515934729460817 1107 68-69 -5.907665881370502 1107 70-71 -5.649554115922509 1107 72-73 -2.0390872310394457 1107 74-75 0.16607579622948165 1107 76-77 0.44108304072171123 1107 78-79 0.2539790859166686 1107 80-81 -0.09681044406141126 1107 82-83 0.3123390218322548 1107 84-85 0.17404375245840242 1107 86-87 0.47736774971642504 1107 88-89 0.15460186906267026 1107 90-91 0.14981945099123095 1107 92-93 0.4183480228049241 1107 94-95 0.962661486990573 1107 96-97 1.2206259375092188 1107 98-99 0.8497426891164892 1107 100-101 0.6900517228807139 1108 1 -0.14962428020065488 1108 2 -0.14888844860891837 1108 3 -0.12059507945848935 1108 4 -0.06992611787778458 1108 5 -0.16363763505032836 1108 6 0.08840345383310932 1108 7 -0.10192614630309293 1108 8 -0.1875303949445879 1108 9 -0.034616677687786535 1108 10-11 -0.0729076866956504 1108 12-13 0.08782225131462695 1108 14-15 -0.028906621546610722 1108 16-17 -0.14919485903795504 1108 18-19 -0.07956442636994865 1108 20-21 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1.7924065485894474 2316 96-97 2.192744079451405 2316 98-99 1.4071325555273688 2316 100-101 1.224330771270548 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 30.0 5 78.0 6 187.0 7 479.0 8 1014.0 9 1635.0 10 2752.0 11 4099.0 12 5331.0 13 6374.0 14 7677.0 15 9635.0 16 11694.0 17 12809.0 18 11403.0 19 9185.0 20 8876.0 21 8872.0 22 9316.0 23 10081.0 24 12274.0 25 14572.0 26 18201.0 27 22579.0 28 27143.0 29 32816.0 30 38577.0 31 44471.0 32 52257.0 33 60212.0 34 70538.0 35 81905.0 36 88102.0 37 80607.0 38 36746.0 39 2054.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.60314547739724 23.956200513927893 15.88974543537798 21.550908573296883 2 14.816186845111638 23.797068984163964 39.56784360622601 21.818900564498385 3 16.073776706148475 29.262978659938604 33.17888614076735 21.484358493145578 4 11.15785679662934 20.20929914614897 40.102909556420045 28.52993450080165 5 10.580694715804858 40.82549081020348 36.070759196010876 12.523055277980779 6 26.4465131309114 41.976907493257436 17.897687020718628 13.678892355112543 7 24.132042406691607 36.565952845548665 23.435041449682448 15.866963298077282 8 24.29560397220945 36.3801439242347 21.24659764600604 18.07765445754981 9 25.6600256031022 16.526181036304205 23.092382455660648 34.721410904932945 10-11 22.440373357859283 29.071763258305474 29.135833157260222 19.352030226575025 12-13 23.44759442697523 27.806336146360255 29.35936315390447 19.38670627276004 14-15 21.370884549894978 28.79777277868231 27.947401782274202 21.88394088914851 16-17 18.48685968542789 31.99289074491819 29.131602006003092 20.388647563650828 18-19 18.2708710212863 29.690390682823363 33.35373739169019 18.685000904200148 20-21 18.923053977802358 29.295541828757504 33.52645446749276 18.254949725947377 22-23 19.159447668999118 28.95145353534098 32.830758522974435 19.058340272685466 24-25 18.99868255881878 29.621670664562078 32.94417032277309 18.435476453846057 26-27 18.508039232623506 29.633954377751024 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19.076372908762984 30.093289383668136 31.29317743640774 19.53716027116114 54-55 19.26658619748282 29.917746066866535 30.773306369316245 20.042361366334404 56-57 19.08112775770778 30.59276845978675 30.386494062617146 19.93960971988832 58-59 18.86218631031 29.47693263926657 31.117084919586667 20.543796130836764 60-61 19.2177847385365 30.602664976196692 30.402108115498876 19.777442169767934 62-63 18.985030624825995 30.17222735950364 31.131366085988148 19.71137592968222 64-65 19.173668049024073 29.49343323263441 31.02958385718112 20.303314861160395 66-67 18.841224618437927 29.978936463010868 30.424984163340707 20.754854755210495 68-69 19.18232393135135 29.121415829840775 31.043771001578307 20.652489237229567 70-71 18.847371952863853 29.3676506556791 31.564849830357534 20.22012756109951 72-73 19.677852599958243 29.02000255524152 30.572280615376947 20.729864229423296 74-75 18.75681135949601 29.86548129042859 30.458563594368798 20.919143755706603 76-77 18.789133539766574 29.692575240193076 30.817681989217572 20.700609230822778 78-79 18.714550831140453 29.587555951109824 30.56095920805174 21.136934009697985 80-81 18.94981496384726 29.939999192117554 29.768044520537302 21.34214132349788 82-83 18.90204949104513 29.849364272486607 29.76416559987074 21.48442063659752 84-85 18.409003343317714 29.442573236058116 29.932325780832475 22.216097639791695 86-87 18.826316374932965 29.555492168958835 30.124977239861717 21.493214216246482 88-89 19.12891522689393 29.07686341869018 29.97416052787593 21.820060826539958 90-91 18.624079845628472 29.081383750594274 30.374338529803836 21.92019787397342 92-93 19.151297681555167 29.14291431628964 29.909784052216743 21.796003949938445 94-95 18.580540277924847 28.33755847197394 30.509445832061637 22.572455418039578 96-97 18.92656035878909 28.253457082347076 30.068643328765415 22.75133923009842 98-99 18.79260289179122 28.442058824077833 29.702672448674527 23.06266583545642 100-101 18.7364514140249 28.136311204804898 30.182209836522016 22.945027544648184 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2318.0 1 1510.5 2 779.0 3 907.5 4 829.0 5 753.5 6 1136.0 7 2027.5 8 2870.0 9 3137.0 10 2848.5 11 2450.5 12 2281.0 13 2199.0 14 2109.0 15 2036.5 16 2030.5 17 2096.0 18 2159.0 19 2247.0 20 2472.0 21 2779.5 22 3135.5 23 3531.5 24 3834.5 25 4665.0 26 6881.5 27 9107.0 28 9844.0 29 11010.0 30 13626.0 31 16583.0 32 21172.0 33 26436.5 34 30521.0 35 33569.0 36 37507.0 37 41714.0 38 41351.5 39 38188.0 40 35443.0 41 32354.0 42 29612.0 43 29185.5 44 28368.0 45 26111.5 46 23090.0 47 20623.0 48 20640.5 49 19845.0 50 17714.0 51 15581.0 52 13694.5 53 12604.0 54 11720.0 55 10184.5 56 8705.0 57 7855.5 58 7338.0 59 6838.5 60 6233.5 61 5441.5 62 4504.0 63 3518.0 64 2596.0 65 1920.0 66 1473.0 67 1176.0 68 888.5 69 683.0 70 560.0 71 476.5 72 439.0 73 418.5 74 385.5 75 326.0 76 215.5 77 129.5 78 81.5 79 50.5 80 34.0 81 17.0 82 10.0 83 6.5 84 3.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024484520066120632 2 0.019761617718341017 3 0.0 4 0.0 5 2.4857380777787446E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.214345194446861E-4 18-19 0.001429299394722778 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.592910675002175E-4 28-29 0.015970867149728434 30-31 0.005033619607501958 32-33 0.01025366957083732 34-35 0.010440099926670726 36-37 0.09955381001503871 38-39 0.10191526118892853 40-41 0.11521395990504481 42-43 0.10191526118892853 44-45 0.09750307610087125 46-47 0.09874594513976061 48-49 0.10092096595781704 50-51 0.09961595346698318 52-53 0.07954361848891982 54-55 0.06904137511030463 56-57 0.03995823960029332 58-59 0.018953752843062926 60-61 0.009942952311114977 62-63 0.001740016654445121 64-65 0.005779341030835581 66-67 0.4278576666376664 68-69 0.8183671186567071 70-71 0.9104015709864651 72-73 0.28803489976261204 74-75 0.15467505188978237 76-77 0.01653015821722865 78-79 0.011558682061671162 80-81 0.0021750208180564014 82-83 0.0 84-85 0.0 86-87 4.3500416361128024E-4 88-89 8.700083272225605E-4 90-91 0.004660758895835146 92-93 0.002547881529723213 94-95 0.23055220671397855 96-97 0.23490224835009135 98-99 0.01746230999639568 100-101 0.009694378503337103 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 804590.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.415981852762055 #Duplication Level Percentage of deduplicated Percentage of total 1 71.10215995248325 17.360290470920162 2 9.749217899599962 4.760734546305114 3 4.417705116046728 3.23587823832753 4 2.7108633573309233 2.647535621516378 5 1.918008209813387 2.341502682212615 6 1.3156296746786966 1.927343415714618 7 0.9730128300751193 1.6629944521133133 8 0.8462440701960996 1.6529503888732373 9 0.651307236769666 1.4312075106186686 >10 5.178352604879666 25.499964220352872 >50 0.6585790969074357 11.12229677983351 >100 0.45890078927096745 20.475684148867767 >500 0.01642597903430756 2.6814380645942797 >1k 0.002566559224110556 1.4861646445411998 >5k 0.0010266236896442225 1.7140148152087575 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7464 0.9276774506270273 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6212 0.772070246958078 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4768 0.5925999577424527 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3285 0.4082824792751588 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1655 0.20569482593619112 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1133 0.14081706210616587 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1017 0.12639978125504914 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 999 0.12416261698504828 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 995 0.12366546936949253 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 896 0.11136106588448774 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 824 0.10241240880448427 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 817 0.10154240047726172 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.214345194446862E-5 0.0 22-23 0.0 0.0 0.0 4.350041636112803E-4 0.0 24-25 0.0 0.0 0.0 0.0029207422413900248 0.0 26-27 0.0 0.0 0.0 0.004660758895835146 0.0 28-29 0.0 0.0 0.0 0.01162082551361563 0.0 30-31 0.0 0.0 0.0 0.033122459886401764 0.0 32-33 0.0 0.0 0.0 0.07121639592836103 0.0 34-35 0.0 0.0 0.0 0.12441119079282617 0.0 36-37 0.0 0.0 0.0 0.2074969860425807 0.0 38-39 0.0 0.0 0.0 0.3273095613915162 0.0 40-41 0.0 0.0 0.0 0.46197442175517967 0.0 42-43 0.0 0.0 0.0 0.5873799077791173 0.0 44-45 0.0 0.0 0.0 0.7290048347605613 0.0 46-47 0.0 0.0 0.0 0.8977864502417381 0.0 48-49 0.0 0.0 0.0 1.0791831864676418 0.0 50-51 0.0 0.0 0.0 1.272822182726606 0.0 52-53 0.0 0.0 0.0 1.45421891895251 0.0 54-55 0.0 0.0 0.0 1.6445643122584173 0.0 56-57 0.0 0.0 0.0 1.8558520488696106 0.0 58-59 0.0 0.0 0.0 2.05943399743969 0.0 60-61 0.0 0.0 0.0 2.2735181893883842 0.0 62-63 0.0 0.0 0.0 2.484495208739855 0.0 64-65 0.0 0.0 0.0 2.6995736959196606 0.0 66-67 0.0 0.0 0.0 2.9244087050547485 0.0 68-69 0.0 0.0 0.0 3.188331945462907 0.0 70-71 0.0 0.0 0.0 3.5560968940702717 0.0 72-73 0.0 0.0 0.0 3.9253532855243045 0.0 74-75 0.0 0.0 0.0 4.330652879106129 0.0 76-77 0.0 0.0 0.0 4.768826358766576 0.0 78-79 0.0 0.0 0.0 5.280080537913721 0.0 80-81 0.0 0.0 0.0 5.824767894206987 0.0 82-83 0.0 0.0 0.0 6.36771523384581 0.0 84-85 0.0 6.214345194446862E-5 0.0 6.910413999676853 0.0 86-87 0.0 1.2428690388893723E-4 0.0 7.460445692837346 0.0 88-89 0.0 1.2428690388893723E-4 0.0 8.0260753924359 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3635 0.0 42.64198 1 GTATCAA 6020 0.0 35.590652 1 ATACGAC 60 0.0040038917 31.579535 3 CTTTAGG 185 9.094947E-12 30.735586 2 CCTTTAG 190 1.2732926E-11 29.937922 1 CATTCCG 200 2.3646862E-11 28.421583 9 ATCAACG 7485 0.0 28.415255 3 TCAACGC 7520 0.0 28.283003 4 AACGCAG 7525 0.0 28.201262 6 CAACGCA 7625 0.0 27.893532 5 CCCTATA 170 3.7343852E-9 27.872957 2 GTCGGTC 35 0.007781475 27.56671 64-65 TATCAAC 7765 0.0 27.399136 2 TAGGCAT 210 4.3655746E-11 27.068174 5 GTACATG 4020 0.0 27.002464 1 GAGTCGG 45 8.2552794E-4 26.756092 62-63 TAAGGCG 95 0.0012460878 24.931213 5 ACGCAGA 8560 0.0 24.6254 7 CGCAGAG 8645 0.0 24.383276 8 ACATGGG 4160 0.0 24.367865 3 >>END_MODULE