FastQCFastQC Report
Fri 27 May 2016
SRR939520_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939520_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804142
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56610.7039801428105982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38430.47790066928477803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30880.38401177901415423No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21520.2676144263077914No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9230.11478072280766331No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGC601.4525594E-647.6836664
ATCGACG607.673673E-540.1043663
GGTATCA31750.039.651891
CATTCCG1950.037.3224269
GTATCAA52350.036.6501661
CGACGCA851.4022335E-534.2795035
TAGGCAT2500.033.022595
CTTTAGG2600.030.1082322
GTACATG35350.029.9155851
CCTTTAG2650.028.504471
TCAACGC69850.028.0572474
AACGCAG68450.027.9277526
ATCAACG69400.027.8765773
CAACGCA69900.027.5118755
TATCAAC68850.027.2165742
TACATGG38300.026.8261992
GTCAACG908.2874263E-426.7362443
ACATGGG37350.026.4141223
GTCCAAG1158.61378E-526.2736871
GGATATG2700.026.1115041
Produced by FastQC (version 0.11.5)