FastQCFastQC Report
Fri 27 May 2016
SRR939520_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939520_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804142
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71900.8941206901268681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58860.7319602756727045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47560.5914378306319034No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31700.39420898299056634No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15990.19884547754003648No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.12647020053672112No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA10150.12622148824461352No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT10080.1253509952222369No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9250.11502943509977093No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8730.108562915504973No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC8520.10595143643784308No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC8110.10085283444963701No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGC250.005061907756.8824849
GGTATCA35550.040.3185771
GTATCAA58250.034.709761
GGATATG1859.094947E-1230.7855831
CATTCCG1751.546141E-1029.7955869
CTCGCAA650.005866468429.2068374
TAGACTA856.483253E-427.8835725
TGGGCGC856.483253E-427.8835726
ATCAACG72450.027.7688053
TCAACGC72300.027.7677044
GTACATG40400.027.4898531
AACGCAG73800.027.3621716
CCTCGCA700.00843391827.1138783
GGACCGC700.0084749827.0868976
CGCAAGT700.0084749827.0868976
CAACGCA74650.026.9236155
TATCAAC75350.026.7017272
CTTTAGG2550.026.0522142
CTCCCGC1301.0177828E-525.5241937
ACATGGG42800.025.0549983
Produced by FastQC (version 0.11.5)