##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939520_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 804142 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.86902686341467 31.0 31.0 31.0 28.0 34.0 2 31.122551738374565 31.0 31.0 34.0 28.0 34.0 3 31.10009674908163 31.0 31.0 34.0 28.0 34.0 4 35.05024237012866 35.0 35.0 37.0 32.0 37.0 5 34.64016305577871 35.0 35.0 37.0 32.0 37.0 6 34.696811010990594 35.0 35.0 37.0 31.0 37.0 7 34.52580514386762 35.0 35.0 37.0 30.0 37.0 8 34.42177500988631 35.0 35.0 37.0 30.0 37.0 9 35.89437562022628 37.0 35.0 39.0 30.0 39.0 10-11 35.705007324577004 37.0 35.0 39.0 30.0 39.0 12-13 35.709924988372705 37.0 35.0 39.0 30.0 39.0 14-15 36.4779647127 38.0 35.0 40.0 30.0 41.0 16-17 36.411007632980244 38.0 35.0 40.0 30.0 41.0 18-19 36.50048498896961 38.0 35.0 40.0 30.0 41.0 20-21 36.404864439365184 38.0 34.5 40.0 30.0 41.0 22-23 36.23020312332896 38.0 34.0 40.0 30.0 41.0 24-25 36.07545868764472 38.0 34.0 40.0 29.0 41.0 26-27 35.60399655782187 38.0 34.0 40.0 27.0 41.0 28-29 35.38274956413171 38.0 34.0 40.0 27.0 41.0 30-31 35.042723797538244 37.0 33.0 40.0 27.0 41.0 32-33 34.68599700052975 37.0 33.0 40.0 25.5 41.0 34-35 34.81597715328885 37.0 33.0 40.0 26.0 41.0 36-37 34.57362940873627 37.0 33.0 40.0 25.0 41.0 38-39 34.350463599712484 37.0 33.0 40.0 24.5 41.0 40-41 34.192372118357206 37.0 33.0 40.0 24.0 41.0 42-43 33.974740779613555 37.0 33.0 40.0 24.0 41.0 44-45 33.52481340360285 37.0 32.5 40.0 22.5 41.0 46-47 33.01962091272437 37.0 31.0 40.0 19.5 41.0 48-49 32.58291072969699 36.5 31.0 39.5 16.5 41.0 50-51 32.352613717477766 36.0 31.0 39.0 15.0 40.5 52-53 32.25974143870113 36.0 31.0 39.0 14.5 40.0 54-55 31.894463913089975 36.0 30.5 39.0 11.0 40.0 56-57 31.40022471155592 35.5 29.5 39.0 8.5 40.0 58-59 30.979595021774763 35.0 29.0 39.0 7.5 40.0 60-61 30.631940005620898 35.0 28.0 38.5 6.5 40.0 62-63 30.45587781759938 35.0 28.0 38.0 2.0 40.0 64-65 30.008265953028197 34.0 28.0 38.0 2.0 40.0 66-67 29.15594074180928 34.0 27.0 37.5 2.0 39.5 68-69 25.77937167813645 31.0 15.5 36.0 2.0 39.0 70-71 24.559037458558315 29.5 10.5 35.0 2.0 38.0 72-73 25.524355151204638 31.0 16.5 35.0 2.0 38.0 74-75 26.21533634606823 31.0 21.0 35.0 2.0 38.0 76-77 25.824810792123778 30.5 21.0 34.5 2.0 36.5 78-79 26.863337569732707 32.0 24.5 35.0 2.0 37.0 80-81 26.838744898289107 32.0 25.0 35.0 2.0 36.5 82-83 26.499708384837504 32.0 24.5 35.0 2.0 36.0 84-85 26.126827724456625 32.0 23.5 35.0 2.0 36.0 86-87 25.7040479169102 32.0 22.5 34.0 2.0 35.5 88-89 25.211973755878937 31.0 20.0 34.0 2.0 35.0 90-91 24.6394542257462 31.0 18.5 34.0 2.0 35.0 92-93 24.097656881496054 31.0 13.5 34.0 2.0 35.0 94-95 23.280801152035338 30.0 4.5 34.0 2.0 35.0 96-97 22.623786594904878 30.0 2.0 34.0 2.0 35.0 98-99 21.83480032133628 29.5 2.0 34.0 2.0 35.0 100-101 20.457804716082485 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3239392259533993 1101 2 0.306203048008328 1101 3 0.1891566327207279 1101 4 0.44194817057898916 1101 5 0.5237074450305599 1101 6 0.38956617802350735 1101 7 0.5013297365903213 1101 8 0.6317702439918875 1101 9 0.5807502095686559 1101 10-11 0.799503086798623 1101 12-13 0.8501183613812628 1101 14-15 0.7897513587380729 1101 16-17 0.7630871006948396 1101 18-19 0.44930264175807366 1101 20-21 0.7373737207929807 1101 22-23 0.9049300013048409 1101 24-25 0.8483828001906204 1101 26-27 0.810077214241602 1101 28-29 0.9377953828089645 1101 30-31 0.842821043630785 1101 32-33 1.1955257531230998 1101 34-35 0.9591265119096235 1101 36-37 1.1471914861265802 1101 38-39 1.4483822193353575 1101 40-41 1.303845770206756 1101 42-43 0.8905458573653817 1101 44-45 0.8938042067956289 1101 46-47 0.986284076847916 1101 48-49 0.8174663318510724 1101 50-51 0.6852562179120198 1101 52-53 0.32711716676563896 1101 54-55 0.22167480311839327 1101 56-57 0.2540256649511399 1101 58-59 0.513834013417739 1101 60-61 0.4386184715201331 1101 62-63 -0.056558609329137255 1101 64-65 -0.17773245845133445 1101 66-67 -0.2649080411364615 1101 68-69 -7.089062379546004 1101 70-71 -7.423848763424147 1101 72-73 -4.6658042286959045 1101 74-75 -2.837760796317742 1101 76-77 -0.679001556800042 1101 78-79 -0.4135331043602335 1101 80-81 -0.40133218080103816 1101 82-83 -0.2594710785213614 1101 84-85 -0.3247365795225967 1101 86-87 -0.5544125618405076 1101 88-89 -0.484504141555508 1101 90-91 -0.46127380094110393 1101 92-93 -0.6050176583536562 1101 94-95 -0.5738516548778172 1101 96-97 -0.784982137063853 1101 98-99 -1.1693600094642669 1101 100-101 -0.5671248212920226 1102 1 0.40925889283360206 1102 2 0.3263888263555401 1102 3 0.32391127717940904 1102 4 0.18437866385252732 1102 5 0.5402609363822606 1102 6 0.45041947719071374 1102 7 0.4177281991143502 1102 8 0.5764569832615862 1102 9 0.6202338738863986 1102 10-11 0.6389284551522394 1102 12-13 0.6790017694530164 1102 14-15 0.929040276098732 1102 16-17 0.7067597464347557 1102 18-19 0.44141795245634796 1102 20-21 0.6085415619204682 1102 22-23 0.7704271185373912 1102 24-25 0.6552924734769761 1102 26-27 0.7425807376240456 1102 28-29 0.5086711035008236 1102 30-31 0.5456455151234181 1102 32-33 0.6491612431935678 1102 34-35 0.5690496381107764 1102 36-37 0.732604042180391 1102 38-39 0.6194148906998649 1102 40-41 0.5655965709755009 1102 42-43 0.2751749412859468 1102 44-45 0.4825665386341953 1102 46-47 0.6469195669183847 1102 48-49 0.6421940704801514 1102 50-51 0.8150127842156749 1102 52-53 0.6265854563236104 1102 54-55 0.6310681407224941 1102 56-57 0.7647514817352494 1102 58-59 0.6812888500609162 1102 60-61 0.9300310275739498 1102 62-63 0.5651665668399808 1102 64-65 0.2401208407158677 1102 66-67 0.41859035732606387 1102 68-69 -6.440110425670284 1102 70-71 -7.239103728190322 1102 72-73 -1.0377843696312006 1102 74-75 0.5121732203382514 1102 76-77 0.8354776232127712 1102 78-79 0.7879672159472619 1102 80-81 0.5633109966493173 1102 82-83 0.5635923423626892 1102 84-85 0.08508981381500647 1102 86-87 -0.0660384426861178 1102 88-89 -0.10294872835883595 1102 90-91 -0.14979013662335916 1102 92-93 -0.1979792214542293 1102 94-95 0.2398190690171198 1102 96-97 0.06965527485158418 1102 98-99 -0.2526585360497897 1102 100-101 -0.12535416145858136 1103 1 0.33374299712629707 1103 2 0.24264919693769826 1103 3 0.27152416228289056 1103 4 0.4076483942926359 1103 5 0.42695448881451625 1103 6 0.4569001505386936 1103 7 0.37211017762579957 1103 8 0.43908376268795024 1103 9 0.4402823284564761 1103 10-11 0.3860329685499764 1103 12-13 0.5177335739092257 1103 14-15 0.6067120490544653 1103 16-17 0.7284025369364571 1103 18-19 0.6754093851265637 1103 20-21 0.5717303842637378 1103 22-23 0.4109140217586642 1103 24-25 0.5402504895482423 1103 26-27 0.7269458623719984 1103 28-29 0.8156215253465078 1103 30-31 0.592621618894448 1103 32-33 0.7336369707645218 1103 34-35 0.5381485636833574 1103 36-37 0.8299834324353057 1103 38-39 0.893747511761056 1103 40-41 0.8370838654448534 1103 42-43 0.6080543265248579 1103 44-45 0.4574865334175513 1103 46-47 0.3949858985161825 1103 48-49 0.5837175303170312 1103 50-51 0.4327634087078067 1103 52-53 0.03687427766369211 1103 54-55 0.06286432053609659 1103 56-57 0.2925689695212874 1103 58-59 0.28456204154174003 1103 60-61 0.22595627912640026 1103 62-63 -0.2239820673029307 1103 64-65 -0.38175419063413685 1103 66-67 -0.37578244190348187 1103 68-69 -7.130354805241112 1103 70-71 -8.226911083654251 1103 72-73 -1.5376175875965075 1103 74-75 0.12836512250616394 1103 76-77 0.20410103188643802 1103 78-79 0.0023786885448480177 1103 80-81 -0.06455940994773002 1103 82-83 -0.003629595619475623 1103 84-85 -0.22352341237654372 1103 86-87 -0.37784560754201024 1103 88-89 -0.12273936047528977 1103 90-91 -0.039473225677443935 1103 92-93 -0.1333116820033844 1103 94-95 0.3348923527923624 1103 96-97 0.37236717581566126 1103 98-99 0.3562852446105218 1103 100-101 -0.10314080083820443 1104 1 0.2709101488358243 1104 2 0.21778332613727613 1104 3 0.27523375029342034 1104 4 0.11426675464978331 1104 5 0.4962611744869463 1104 6 0.28201497701213185 1104 7 0.35390622205176925 1104 8 0.29120260808796417 1104 9 0.5801231552070831 1104 10-11 0.4126769173928082 1104 12-13 0.38913163571754694 1104 14-15 0.6613758846546318 1104 16-17 0.638277366181569 1104 18-19 0.5566060551588379 1104 20-21 0.49852479538211014 1104 22-23 0.7185153363238115 1104 24-25 0.5152645953865758 1104 26-27 0.9435038893364194 1104 28-29 0.5855317924170507 1104 30-31 0.6855568211276264 1104 32-33 0.6481964231610249 1104 34-35 0.5947649442737202 1104 36-37 0.9929392737371998 1104 38-39 0.736140752837251 1104 40-41 0.71189949966314 1104 42-43 0.6120831519292196 1104 44-45 0.8730210209549227 1104 46-47 0.6116627114876181 1104 48-49 0.577295661813146 1104 50-51 0.5457808702508302 1104 52-53 0.34980363958485583 1104 54-55 0.40668175634216297 1104 56-57 0.6687481681116942 1104 58-59 0.9909762384493419 1104 60-61 0.9381538697502272 1104 62-63 0.7344217952220617 1104 64-65 0.6237074909797684 1104 66-67 0.6176880397737783 1104 68-69 -5.890299421265347 1104 70-71 -3.714876576319213 1104 72-73 0.6418556954507473 1104 74-75 1.3586924274496432 1104 76-77 1.3684377605198073 1104 78-79 0.7842096263603722 1104 80-81 0.5728277433456164 1104 82-83 0.28142588734463203 1104 84-85 0.5901768212359357 1104 86-87 0.5334818755804811 1104 88-89 0.548426326206819 1104 90-91 0.48155932168847926 1104 92-93 0.4346277272342114 1104 94-95 0.3858070050463276 1104 96-97 0.4288882801295735 1104 98-99 0.6658972598151287 1104 100-101 0.9406330800986211 1105 1 0.09852672004695506 1105 2 0.15607546886200296 1105 3 0.1141476549289493 1105 4 0.23181366120743263 1105 5 0.4121245185854505 1105 6 0.11244033143349697 1105 7 0.25855638645644774 1105 8 0.5129647628137448 1105 9 0.4740650623013636 1105 10-11 0.42402852387532874 1105 12-13 0.46064096336583304 1105 14-15 0.46712102325216165 1105 16-17 0.43152811502780253 1105 18-19 0.4705387419313283 1105 20-21 0.7300201218010116 1105 22-23 0.563387081159938 1105 24-25 0.6832897146653352 1105 26-27 0.9470109045865414 1105 28-29 0.5435140786665755 1105 30-31 0.38795508048698224 1105 32-33 0.6622921730317444 1105 34-35 0.5732484208716713 1105 36-37 0.7984020704705799 1105 38-39 0.6055231864167823 1105 40-41 0.6188736486128121 1105 42-43 0.5292612456154941 1105 44-45 0.7191581207978359 1105 46-47 1.0761199565140132 1105 48-49 0.8692225139269922 1105 50-51 0.7403357579970802 1105 52-53 0.4245309327130329 1105 54-55 0.7867389077017926 1105 56-57 0.6842842883563982 1105 58-59 0.9119357617245569 1105 60-61 1.241418912534467 1105 62-63 0.8959365867647371 1105 64-65 0.647593734649945 1105 66-67 0.5958013619191362 1105 68-69 -3.26821248192433 1105 70-71 -3.1930710031469367 1105 72-73 0.6373735945833552 1105 74-75 1.3146172159676617 1105 76-77 1.349942843892979 1105 78-79 0.9523871980495926 1105 80-81 0.5957023128200056 1105 82-83 0.3792228890325866 1105 84-85 0.5415377943945536 1105 86-87 0.2581985580208901 1105 88-89 0.3778162238563674 1105 90-91 0.28066729214442176 1105 92-93 -0.014025691114717631 1105 94-95 -0.1295675156432914 1105 96-97 0.15475262431589343 1105 98-99 0.30546500234861895 1105 100-101 0.3500489068541448 1106 1 0.09211505012922316 1106 2 0.04422502603030409 1106 3 0.12115663272073007 1106 4 0.1727613573921758 1106 5 0.2208503021734174 1106 6 0.270357386814716 1106 7 0.2143846816452708 1106 8 0.3112867275083673 1106 9 0.18848647330491985 1106 10-11 0.28640418569972326 1106 12-13 0.22014033940324396 1106 14-15 0.17689421588092102 1106 16-17 0.3486695182772621 1106 18-19 0.31306088351631445 1106 20-21 0.13031877573803996 1106 22-23 0.24005088042571998 1106 24-25 0.274932250740072 1106 26-27 0.31478051094489956 1106 28-29 0.5314437344573122 1106 30-31 0.361546318356055 1106 32-33 0.41255872015607054 1106 34-35 0.3117638745469904 1106 36-37 0.26448818942328245 1106 38-39 0.07686573581887757 1106 40-41 0.31217544053642854 1106 42-43 0.5316008024203285 1106 44-45 0.6421338771252962 1106 46-47 0.5721302306940714 1106 48-49 0.5861476505323893 1106 50-51 0.2962452289010358 1106 52-53 -0.007827888179420484 1106 54-55 -0.03790761446402513 1106 56-57 0.40081687374234676 1106 58-59 0.34935049693422116 1106 60-61 0.25037671327837785 1106 62-63 -0.3089981697686994 1106 64-65 0.2861576514387778 1106 66-67 0.17955349732507742 1106 68-69 -2.5727546872383122 1106 70-71 -3.099980631556015 1106 72-73 0.6400639031722299 1106 74-75 0.9191842586273147 1106 76-77 0.9260753662768799 1106 78-79 0.5265987637716307 1106 80-81 0.12172276425390649 1106 82-83 0.10769375664347436 1106 84-85 0.31519748641146705 1106 86-87 0.14516985574190855 1106 88-89 0.2616277265763607 1106 90-91 0.25802290235559866 1106 92-93 0.31939992406393003 1106 94-95 0.684280213254052 1106 96-97 0.5989079728262574 1106 98-99 0.7474092213049666 1106 100-101 1.1056663874991877 1107 1 -0.2002463719178813 1107 2 0.011188911969743032 1107 3 0.19208628514048698 1107 4 -0.05828931486920652 1107 5 0.2909983148126969 1107 6 0.2005982751464117 1107 7 0.32158318744680514 1107 8 0.23872983155217042 1107 9 0.2796811924390994 1107 10-11 0.09668319656081081 1107 12-13 0.294388442871373 1107 14-15 0.12218774656444964 1107 16-17 0.06182051163779789 1107 18-19 0.3324451662882595 1107 20-21 0.15278100501051028 1107 22-23 0.38733944750474336 1107 24-25 0.36103671670484516 1107 26-27 0.6295190901770766 1107 28-29 0.3808181667770256 1107 30-31 0.45490685770030126 1107 32-33 0.6059697910409483 1107 34-35 0.4925906728941598 1107 36-37 0.20269323234311543 1107 38-39 0.45040383915907256 1107 40-41 0.2306616690925054 1107 42-43 0.025112296746719664 1107 44-45 0.22320118002031109 1107 46-47 0.3375756118553994 1107 48-49 0.21505488997513567 1107 50-51 0.3532089869125272 1107 52-53 -0.02349650361519906 1107 54-55 0.24582314837034502 1107 56-57 0.7492267293116903 1107 58-59 0.663672031046211 1107 60-61 0.7461621269334096 1107 62-63 0.6373219827214172 1107 64-65 -0.0031520493377463765 1107 66-67 0.3327849573667798 1107 68-69 -2.4083352882438263 1107 70-71 -2.443240747458738 1107 72-73 0.9672095747300062 1107 74-75 0.8373225231567325 1107 76-77 0.4232961315418855 1107 78-79 0.297532254029921 1107 80-81 0.26292260050613336 1107 82-83 0.4280752377932906 1107 84-85 0.14691018581916282 1107 86-87 -0.0750984091712894 1107 88-89 0.32508574701924786 1107 90-91 0.4171077057943009 1107 92-93 0.24711871284209153 1107 94-95 0.5903126557824159 1107 96-97 0.5914209894952691 1107 98-99 0.8157003597358035 1107 100-101 1.1075686761302315 1108 1 0.08197721108965084 1108 2 0.2381270777358253 1108 3 0.14848941554484085 1108 4 0.08652620526106602 1108 5 -0.01696968131130916 1108 6 -0.04000277325642543 1108 7 0.43167490587190827 1108 8 0.2186042654617424 1108 9 0.3784248586190273 1108 10-11 0.13886559712067026 1108 12-13 0.1900952399939797 1108 14-15 0.23531700696268132 1108 16-17 0.3276188926023593 1108 18-19 0.47605078378945365 1108 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0.3879852249886788 2315 86-87 0.5136776637234028 2315 88-89 0.3164281892715586 2315 90-91 0.3434480035701739 2315 92-93 -0.7355740493310954 2315 94-95 -0.031596015780070985 2315 96-97 0.11420583286096075 2315 98-99 -2.057420159840202 2315 100-101 -3.4783127912168332 2316 1 -0.36105237068525753 2316 2 -0.2653562423744944 2316 3 -0.6486846371205424 2316 4 -0.2264701300746097 2316 5 -0.08321132247644414 2316 6 -0.45010515717723365 2316 7 -0.7120894977700871 2316 8 -1.1120523517130678 2316 9 -5.035427194726399 2316 10-11 -1.4935893501761655 2316 12-13 -0.6488947105141563 2316 14-15 -0.4294549904682796 2316 16-17 -0.5674423110698683 2316 18-19 -0.4202939968973851 2316 20-21 -3.5159894911584644 2316 22-23 -1.3363389062581845 2316 24-25 -0.46210179364690873 2316 26-27 -0.7110777810898625 2316 28-29 -0.12517660598656022 2316 30-31 -0.10881387700413825 2316 32-33 -1.278097029323206 2316 34-35 -0.7036979512089587 2316 36-37 -0.19142289295838566 2316 38-39 -4.856844671420944 2316 40-41 -4.63677234370547 2316 42-43 -3.7937529288157563 2316 44-45 -2.451235008890645 2316 46-47 -2.3595870716114646 2316 48-49 -2.656133107924841 2316 50-51 -1.276765257344742 2316 52-53 -1.2161414700224142 2316 54-55 -0.030059090439028324 2316 56-57 -3.0908641576445675 2316 58-59 -0.45298017892586273 2316 60-61 -0.4498782605060079 2316 62-63 -0.02005720876891459 2316 64-65 0.21459620634792032 2316 66-67 0.13051772917166105 2316 68-69 2.8458153048610946 2316 70-71 3.7281652421780933 2316 72-73 2.6111136051042863 2316 74-75 1.338025384821382 2316 76-77 -1.320414255212743 2316 78-79 0.32515690497683636 2316 80-81 0.5710889209730077 2316 82-83 0.8282945610678105 2316 84-85 0.8678208434465908 2316 86-87 1.0741327229400746 2316 88-89 0.6070112646069568 2316 90-91 0.698633762084107 2316 92-93 0.6283455535977929 2316 94-95 0.7653546943285328 2316 96-97 0.38922794696976126 2316 98-99 -0.11034413644839347 2316 100-101 0.4780376436939697 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 29.0 5 96.0 6 222.0 7 519.0 8 1071.0 9 1820.0 10 2996.0 11 4164.0 12 5520.0 13 6845.0 14 8222.0 15 10290.0 16 12431.0 17 13354.0 18 11563.0 19 9803.0 20 9730.0 21 9878.0 22 10353.0 23 11421.0 24 13017.0 25 15539.0 26 19321.0 27 23790.0 28 28733.0 29 34110.0 30 40042.0 31 46178.0 32 53346.0 33 61935.0 34 71231.0 35 80373.0 36 83088.0 37 70765.0 38 30764.0 39 1576.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.391694413729546 24.169858045321515 15.961785546817184 21.47666199413176 2 14.736724274102771 24.01502478218419 39.387317243051974 21.860933700661064 3 16.074211050577077 29.385715653636996 33.215650528080346 21.324422767705585 4 11.164774212500902 20.166897777600745 40.26222170218804 28.40610630771031 5 10.533338639195565 40.895886547400835 36.12657963394525 12.444195179458355 6 26.46734034536189 42.09704256213455 17.871097393246465 13.564519699257097 7 24.033690567088897 36.632709148384244 23.416511014223858 15.917089270303006 8 24.3588818890196 36.54926617438214 21.148876690932696 17.942975245665565 9 25.561903245944123 16.625421878225488 23.238681725366913 34.57399315046348 10-11 22.400389029081417 29.054179264302228 29.252424902338525 19.293006804277827 12-13 23.42652168398119 27.806220791850194 29.390953339086877 19.37630418508174 14-15 21.25725307221859 28.781483867277174 28.085649052033094 21.875614008471143 16-17 18.51730488710257 32.01606193969436 29.063393426741964 20.403239746461114 18-19 18.330056544809928 29.66357889972032 33.25051203706813 18.75585251840162 20-21 18.898714406162096 29.23619211532292 33.50092396616518 18.36416951234981 22-23 19.2461774451387 28.976989746207604 32.766621297371174 19.01021151128253 24-25 18.982181586644646 29.711470541417118 32.855995184750384 18.450352687187856 26-27 18.52732049309021 29.65449486234824 33.09219792006716 18.725986724494394 28-29 18.379258629180285 29.218150751369176 33.88809299965677 18.514497619793765 30-31 18.84425813118711 29.16588684951591 33.27965289690931 18.710202122387663 32-33 18.559590717779688 29.731905969051393 33.01479407490864 18.69370923826028 34-35 18.85667704238541 29.46677179180745 33.00666193271908 18.669889233088057 36-37 19.51346561001323 29.000693288790252 32.18403394501237 19.301807156184147 38-39 18.407642796723838 30.13972664640488 32.49570375884534 18.95692679802594 40-41 18.249784529028684 29.40088179744795 33.61925890431844 18.73007476920492 42-43 18.440827660370243 29.47461900029311 32.804838660494084 19.27971467884256 44-45 18.56479559800287 29.52155911947611 32.540464382817426 19.373180899703595 46-47 19.169207198495034 29.649266590621842 31.62780104135357 19.55372516952955 48-49 18.86271271554688 29.964586560067502 31.38666236884862 19.786038355537 50-51 18.848594329777907 29.884438780431193 31.096490600344612 20.170476289446288 52-53 18.95043405278316 30.24171697381871 31.182926821680923 19.624922151717204 54-55 19.313875833237425 29.99598739606889 30.72155328209228 19.968583488601404 56-57 19.13565120039806 30.602935688518475 30.384065182236597 19.877347928846874 58-59 18.835984693239762 29.489208709156006 31.164108595157586 20.51069800244664 60-61 19.205614712377205 30.585158150095054 30.44069845609091 19.768528681436838 62-63 18.99937074381563 30.189311598708656 31.0213350179823 19.789982639493413 64-65 19.150661404367327 29.55466745099551 31.03013427071409 20.264536873923074 66-67 18.938795693353196 29.977131526109417 30.255576370240096 20.82849641029729 68-69 19.484397186664403 29.09255583484024 30.689384975875946 20.733662002619415 70-71 19.101566249263673 29.280774975643997 31.356728059054127 20.2609307160382 72-73 19.727995586823873 29.067803908206933 30.496220219485622 20.707980285483572 74-75 18.999742442170803 29.851201252203865 30.162572492574995 20.986483813050345 76-77 18.384815710120368 29.727739427318266 31.11346474942059 20.773980113140777 78-79 18.820213713987926 29.569157923604994 30.44612779432314 21.164500568083945 80-81 18.906958466088142 29.81672996614908 29.799319499473953 21.476992068288826 82-83 18.972974451828563 29.715978053245255 29.793080206136334 21.517967288789844 84-85 18.41732684440946 29.30157018775971 29.981302995313623 22.299799972517206 86-87 18.813885712544685 29.567422336052452 30.110623829856557 21.50806812154631 88-89 19.091013663708008 29.05417294927795 29.974164866083225 21.880648520930812 90-91 18.619657022190523 29.06655789141834 30.34661539638194 21.967169690009197 92-93 19.09972416297516 29.051123121808537 29.994975736788305 21.854176978428004 94-95 18.75800013067788 28.244965012554413 30.39828749754979 22.598747359217924 96-97 19.167959434259192 28.260417923668037 29.784432299824214 22.787190342248557 98-99 18.73643153908867 28.403860827624033 29.652330246155433 23.20737738713186 100-101 18.75108827055774 28.062953031486465 30.20062734133637 22.98533135661942 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2126.0 1 1439.5 2 807.0 3 870.0 4 773.0 5 744.5 6 1122.5 7 1996.5 8 2854.5 9 3101.0 10 2733.0 11 2290.5 12 2180.0 13 2137.5 14 2069.0 15 2060.0 16 2036.0 17 2040.0 18 2129.5 19 2239.5 20 2441.0 21 2735.5 22 3103.0 23 3526.0 24 3876.5 25 4705.5 26 6991.0 27 9322.5 28 9915.0 29 10967.0 30 13632.5 31 16491.0 32 21048.5 33 26327.0 34 30477.5 35 33878.5 36 37517.0 37 41581.5 38 41806.5 39 38670.5 40 35397.5 41 32142.0 42 29420.5 43 28927.0 44 28397.0 45 26235.5 46 23016.5 47 20561.5 48 20693.5 49 19814.5 50 17716.5 51 15599.0 52 13695.5 53 12541.0 54 11567.5 55 10138.5 56 8687.0 57 7738.0 58 7233.5 59 6837.0 60 6217.0 61 5482.5 62 4637.0 63 3604.0 64 2591.5 65 1937.0 66 1543.5 67 1243.5 68 912.5 69 699.0 70 582.5 71 477.5 72 458.0 73 433.5 74 363.0 75 305.0 76 237.5 77 149.0 78 70.0 79 45.0 80 37.5 81 22.5 82 12.0 83 12.0 84 7.0 85 1.5 86 0.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019026490346232383 2 0.01703679200937148 3 3.7306843816141926E-4 4 0.09650036933775377 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01106769699878877 12-13 0.0 14-15 0.0 16-17 6.217807302690321E-4 18-19 1.2435614605380642E-4 20-21 0.0 22-23 6.217807302690321E-5 24-25 0.0037306843816141922 26-27 0.0036685063085872896 28-29 0.0012435614605380643 30-31 6.217807302690321E-5 32-33 3.108903651345161E-4 34-35 0.0026114790671299347 36-37 0.09015820588900965 38-39 0.10240728627530958 40-41 0.0832564397830234 42-43 0.08661405572647618 44-45 0.08561920655804571 46-47 0.0831320836369696 48-49 0.07877961852508637 50-51 0.07815783779481733 52-53 0.06161847036966108 54-55 0.052789183999840825 56-57 0.030094187345021152 58-59 0.02281935280087348 60-61 0.013865710284999416 62-63 0.0022384106289685154 64-65 0.0071504783980938695 66-67 0.1604194284094103 68-69 0.7302814677009782 70-71 0.8842343765155904 72-73 0.3612546042863076 74-75 0.05459234811762101 76-77 0.19921854597819785 78-79 0.015357984037645092 80-81 0.0034819720895065795 82-83 0.0017409860447532898 84-85 3.108903651345161E-4 86-87 0.0025493009941030317 88-89 5.596026572421289E-4 90-91 0.0046633554770177405 92-93 0.005471670426367483 94-95 0.0795257554014092 96-97 0.038674761422733794 98-99 0.014611847161322253 100-101 0.014176600650133932 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 804142.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.165432134641495 #Duplication Level Percentage of deduplicated Percentage of total 1 71.82877255184735 18.076021009681142 2 9.60775660239572 4.8356669348748635 3 4.365968150678559 3.2961442559372256 4 2.565186530238872 2.5821611015769133 5 1.8247477545828221 2.2960282890396733 6 1.309461383257488 1.9771896943980054 7 1.0009009973507763 1.7631674285628167 8 0.7802513428766678 1.570828977370054 9 0.6468263272831223 1.4649897637928542 >10 4.985567183977247 25.423799247233703 >50 0.6319887147269523 11.026325774972156 >100 0.4324133311281497 19.846219574540225 >500 0.0161273037250592 2.589453448363621 >1k 0.0030238694484485996 1.5935976185869078 >5k 0.0010079564828162 1.6584068810698294 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7190 0.8941206901268681 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5886 0.7319602756727045 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4756 0.5914378306319034 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3170 0.39420898299056634 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1599 0.19884547754003648 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1017 0.12647020053672112 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1015 0.12622148824461352 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1008 0.1253509952222369 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 925 0.11502943509977093 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 873 0.108562915504973 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 852 0.10595143643784308 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 811 0.10085283444963701 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.2435614605380642E-4 0.0 22-23 0.0 0.0 0.0 6.21780730269032E-4 0.0 24-25 0.0 0.0 0.0 0.0014300956796187737 0.0 26-27 0.0 0.0 0.0 0.0037306843816141927 0.0 28-29 0.0 0.0 0.0 0.010881162779708062 0.0 30-31 0.0 0.0 0.0 0.03258131026609728 0.0 32-33 0.0 0.0 0.0 0.06920419527894327 0.0 34-35 0.0 0.0 0.0 0.12659455668277494 0.0 36-37 0.0 0.0 0.0 0.21034842105001356 0.0 38-39 0.0 0.0 0.0 0.32631052724518805 0.0 40-41 0.0 0.0 0.0 0.47106108125181867 0.0 42-43 0.0 0.0 0.0 0.5923083236542799 0.0 44-45 0.0 0.0 0.0 0.733639083644431 0.0 46-47 0.0 0.0 0.0 0.9059345240019798 0.0 48-49 0.0 0.0 0.0 1.0861265796339452 0.0 50-51 0.0 0.0 0.0 1.290443727600349 0.0 52-53 0.0 0.0 0.0 1.4731229061533908 0.0 54-55 0.0 0.0 0.0 1.65866227606567 0.0 56-57 0.0 0.0 0.0 1.8719330665479479 0.0 58-59 0.0 0.0 0.0 2.0871313772940603 0.0 60-61 6.217807302690321E-5 0.0 0.0 2.312713426235665 0.0 62-63 1.2435614605380642E-4 0.0 0.0 2.515040875865208 0.0 64-65 1.2435614605380642E-4 0.0 0.0 2.7391928291271936 0.0 66-67 1.2435614605380642E-4 0.0 0.0 2.9693138773997627 0.0 68-69 1.2435614605380642E-4 0.0 0.0 3.232513660522644 0.0 70-71 1.2435614605380642E-4 0.0 0.0 3.5734360349291543 0.0 72-73 1.2435614605380642E-4 0.0 0.0 3.9369290498444305 0.0 74-75 1.2435614605380642E-4 0.0 0.0 4.324484979021118 0.0 76-77 1.2435614605380642E-4 0.0 0.0 4.771483146011525 0.0 78-79 1.2435614605380642E-4 0.0 0.0 5.275685140186684 0.0 80-81 1.2435614605380642E-4 0.0 0.0 5.827764250592557 0.0 82-83 1.2435614605380642E-4 0.0 0.0 6.384506716475448 0.0 84-85 1.2435614605380642E-4 0.0 0.0 6.923714965764753 0.0 86-87 1.2435614605380642E-4 0.0 0.0 7.487110485461523 0.0 88-89 1.2435614605380642E-4 0.0 0.0 8.07487980978484 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATACGC 25 0.0050619077 56.882484 9 GGTATCA 3555 0.0 40.318577 1 GTATCAA 5825 0.0 34.70976 1 GGATATG 185 9.094947E-12 30.785583 1 CATTCCG 175 1.546141E-10 29.795586 9 CTCGCAA 65 0.0058664684 29.206837 4 TAGACTA 85 6.483253E-4 27.883572 5 TGGGCGC 85 6.483253E-4 27.883572 6 ATCAACG 7245 0.0 27.768805 3 TCAACGC 7230 0.0 27.767704 4 GTACATG 4040 0.0 27.489853 1 AACGCAG 7380 0.0 27.362171 6 CCTCGCA 70 0.008433918 27.113878 3 GGACCGC 70 0.00847498 27.086897 6 CGCAAGT 70 0.00847498 27.086897 6 CAACGCA 7465 0.0 26.923615 5 TATCAAC 7535 0.0 26.701727 2 CTTTAGG 255 0.0 26.052214 2 CTCCCGC 130 1.0177828E-5 25.524193 7 ACATGGG 4280 0.0 25.054998 3 >>END_MODULE