FastQCFastQC Report
Fri 27 May 2016
SRR939519_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939519_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1513338
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183581.2130799596653226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145150.9591380114686872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97970.6473768583092475No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA92590.6118263071435462No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84940.5612758022332089No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA68640.45356688327392825No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33720.22281869615380043No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30820.2036557596518425No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG30690.20279673146382368No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT30460.20127691236194425No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT30150.19922846052897636No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA29950.19790687870125512No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27360.1807923940322651No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26100.1724664285176213No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC23500.15528586475724523No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG23250.1536338874725937No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19920.1316295500410351No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG19350.1278630418320296No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18230.12046218359679067No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT15290.1010349307292885No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15150.10010982344988363No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG168400.026.0749242
GTACATG169550.025.8159981
ATGGGAG34900.025.521245
GAGTACT155050.025.12820612-13
ACATGGG177250.023.8680423
AGTACTT156100.023.7611412-13
GTACTTT171300.022.9828914-15
CATGGGG46500.022.777584
AACGTAG1953.3413198E-722.4626146
CATGGGA123850.022.095734
TAGCCCT6600.021.7843974
ACTTTTT196300.021.5073816-17
TACTTTT179800.020.77720314-15
TACACGG2601.0542863E-820.710572
GTATAGC4750.020.147771
ATGGGGG19800.020.0474015
GTATCAA393350.019.9889851
CGCATAG5200.019.8766468
GATAGTC2251.3139688E-619.4796057
GCCGAGT4350.019.2594369
Produced by FastQC (version 0.11.5)