##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939519_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1513338 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.01921315661141 31.0 31.0 31.0 30.0 34.0 2 31.223471557576694 31.0 31.0 34.0 30.0 34.0 3 31.174394616404268 31.0 31.0 34.0 28.0 34.0 4 35.125216574222016 35.0 35.0 37.0 33.0 37.0 5 34.78480683099215 35.0 35.0 37.0 32.0 37.0 6 34.792376190910424 35.0 35.0 37.0 32.0 37.0 7 34.67126577142714 35.0 35.0 37.0 31.0 37.0 8 34.72070482602036 35.0 35.0 37.0 31.0 37.0 9 35.97739037809134 37.0 35.0 39.0 30.0 39.0 10-11 35.84065886140439 37.0 35.0 39.0 30.0 39.0 12-13 35.78886805194874 37.0 35.0 39.0 30.0 39.0 14-15 36.43019206548702 38.0 35.0 40.0 30.0 41.0 16-17 36.51075800647311 38.0 35.0 40.0 30.5 41.0 18-19 36.67376620424518 38.5 35.0 40.0 30.5 41.0 20-21 36.65785799338944 38.5 35.0 40.0 30.0 41.0 22-23 36.52342602908273 38.0 35.0 40.0 30.0 41.0 24-25 36.2660136070065 38.0 34.5 40.0 29.5 41.0 26-27 35.803926485689246 38.0 34.0 40.0 28.0 41.0 28-29 35.49791355268948 38.0 34.0 40.0 27.0 41.0 30-31 35.164417664791344 37.0 34.0 40.0 27.0 41.0 32-33 34.76049302931665 37.0 33.0 40.0 27.0 41.0 34-35 34.513496654415604 37.0 33.0 40.0 25.5 41.0 36-37 34.29517992675794 37.0 33.0 40.0 24.5 41.0 38-39 34.1427430620258 37.0 33.0 40.0 24.0 41.0 40-41 33.7948181437326 37.0 33.0 40.0 23.5 41.0 42-43 33.35765572529071 36.5 32.5 40.0 21.5 41.0 44-45 32.95627645641621 36.0 32.0 40.0 19.5 41.0 46-47 32.286393059580874 36.0 31.0 40.0 12.0 41.0 48-49 31.74349781740761 36.0 30.5 39.5 7.5 41.0 50-51 31.39076994035701 36.0 30.0 39.0 2.0 41.0 52-53 30.94691073639861 35.0 29.0 39.0 2.0 40.0 54-55 30.741768857981498 35.0 29.0 39.0 2.0 40.0 56-57 30.40059656203703 35.0 28.0 39.0 2.0 40.0 58-59 30.027961037124555 35.0 28.0 38.5 2.0 40.0 60-61 29.737620743019736 35.0 27.5 38.0 2.0 40.0 62-63 29.33055074279507 34.0 27.0 38.0 2.0 40.0 64-65 28.87869266482438 34.0 26.0 38.0 2.0 40.0 66-67 27.397905491040333 33.0 22.0 36.5 2.0 39.5 68-69 24.179114645901972 29.5 10.5 35.0 2.0 38.0 70-71 23.64261387740214 28.5 10.5 35.0 2.0 38.0 72-73 25.20495057944755 31.0 16.5 35.0 2.0 37.5 74-75 25.69558452903449 31.0 20.0 35.0 2.0 37.0 76-77 25.572014645769812 30.5 21.0 34.0 2.0 36.0 78-79 26.303092237160502 32.0 23.0 35.0 2.0 37.0 80-81 26.16795487855324 32.0 22.5 35.0 2.0 36.0 82-83 25.77909495433274 32.0 20.5 35.0 2.0 36.0 84-85 25.459002549331345 32.0 20.0 35.0 2.0 36.0 86-87 24.977206347821834 32.0 18.5 34.5 2.0 35.0 88-89 24.43201915236385 31.0 14.5 34.0 2.0 35.0 90-91 23.949732643996253 31.0 7.5 34.0 2.0 35.0 92-93 23.485359516512503 31.0 2.0 34.0 2.0 35.0 94-95 22.298550951604994 30.0 2.0 34.0 2.0 35.0 96-97 21.533100668852562 29.0 2.0 34.0 2.0 35.0 98-99 21.207143744490658 29.0 2.0 34.0 2.0 35.0 100-101 20.088020323285345 27.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.29983712548459707 1101 2 0.29633141852440303 1101 3 0.33638502398549264 1101 4 0.2702648327836812 1101 5 0.3672350695396034 1101 6 0.6227304787912828 1101 7 0.631969506376592 1101 8 0.4434506858820768 1101 9 0.7198338534061861 1101 10-11 0.7722941124478098 1101 12-13 0.712489854550924 1101 14-15 0.7415692841341368 1101 16-17 0.7569923779629875 1101 18-19 0.7984807720960774 1101 20-21 0.8879559890251159 1101 22-23 0.945420582133913 1101 24-25 0.7553201261101705 1101 26-27 0.9494229624579802 1101 28-29 0.7923568509807026 1101 30-31 0.8284401909402064 1101 32-33 0.7545884314566038 1101 34-35 0.865030650314246 1101 36-37 0.9321455221675379 1101 38-39 0.9403839459870298 1101 40-41 1.1188472601654453 1101 42-43 1.2791728272145448 1101 44-45 0.87897512557889 1101 46-47 0.8463873919865534 1101 48-49 0.4629789804636637 1101 50-51 0.36015581115589157 1101 52-53 0.3845398614369664 1101 54-55 0.18337678355968734 1101 56-57 0.2949461463434204 1101 58-59 0.3591538262021565 1101 60-61 0.23337564611342643 1101 62-63 0.1418729580221374 1101 64-65 -0.009105687049750344 1101 66-67 -2.604295446646198 1101 68-69 -7.255765721878877 1101 70-71 -10.380163519959172 1101 72-73 -10.47503882989145 1101 74-75 -9.046962104722905 1101 76-77 -4.526410714627222 1101 78-79 -1.7444999973469457 1101 80-81 -1.122695972962159 1101 82-83 -0.8792725877364873 1101 84-85 -0.7615972269759403 1101 86-87 -0.7708634278728788 1101 88-89 -0.7407282994027966 1101 90-91 -0.683025315681423 1101 92-93 -1.2299986733809618 1101 94-95 -4.313163923446119 1101 96-97 -1.6444098224047075 1101 98-99 -1.1939175813939293 1101 100-101 -0.6888862057675773 1102 1 0.407653555170441 1102 2 0.35700023106100787 1102 3 0.427743221848484 1102 4 0.3439860468735816 1102 5 0.39811886280767084 1102 6 0.422700702385022 1102 7 0.5749169276413753 1102 8 0.5413855321521481 1102 9 0.6428790692482167 1102 10-11 0.6585666969906612 1102 12-13 0.6515681886471256 1102 14-15 0.5066654299351754 1102 16-17 0.6745258825721834 1102 18-19 0.7219535623668207 1102 20-21 0.9592680211541023 1102 22-23 0.9146395297717262 1102 24-25 0.7893845809558471 1102 26-27 0.695558797009447 1102 28-29 0.75345680619305 1102 30-31 0.4799448838002647 1102 32-33 0.46445303493823786 1102 34-35 0.8288966121906043 1102 36-37 0.9055663882918239 1102 38-39 0.8600553227397825 1102 40-41 0.9902070329788515 1102 42-43 1.1137347449135362 1102 44-45 0.8595663225904175 1102 46-47 1.06298921499166 1102 48-49 0.7599032745659677 1102 50-51 0.8106100859158332 1102 52-53 0.8948705518066369 1102 54-55 0.6168960348676329 1102 56-57 0.5588130925637884 1102 58-59 0.3526811491309729 1102 60-61 0.2315256945923707 1102 62-63 0.1761367425295468 1102 64-65 0.16330893341180897 1102 66-67 -2.3980230835807532 1102 68-69 -7.369734916833128 1102 70-71 -10.428328087512241 1102 72-73 -8.610815551139993 1102 74-75 -7.361970643907178 1102 76-77 -3.8688043193442 1102 78-79 -1.4617262635824666 1102 80-81 -0.8403479679813763 1102 82-83 -0.6411788437348349 1102 84-85 -0.4872125650242829 1102 86-87 -0.4801971008048973 1102 88-89 -0.4495378091502644 1102 90-91 -0.37957851209250393 1102 92-93 -0.5423144164868638 1102 94-95 -3.5018276026287154 1102 96-97 -1.0053638486177867 1102 98-99 -0.4451713895748135 1102 100-101 -0.36302342824409095 1103 1 0.3306237147818223 1103 2 0.3047265399514387 1103 3 0.4188855612732638 1103 4 0.28684016052922345 1103 5 0.38879374136423195 1103 6 0.45751448910720427 1103 7 0.492489600939237 1103 8 0.45557995731985557 1103 9 0.6314768794109114 1103 10-11 0.8641434569567323 1103 12-13 0.6538717586987914 1103 14-15 0.8221557337365439 1103 16-17 0.8603463968755136 1103 18-19 0.7525023710644874 1103 20-21 0.8219501863171814 1103 22-23 0.705407418583512 1103 24-25 0.839694454500453 1103 26-27 0.9480864591267988 1103 28-29 0.8196242053757103 1103 30-31 0.9238423508370417 1103 32-33 0.8950085904937879 1103 34-35 1.1321365497339713 1103 36-37 1.0439685395756655 1103 38-39 1.1972343650714947 1103 40-41 1.2308650443906757 1103 42-43 1.3445808972768631 1103 44-45 1.1904274144247964 1103 46-47 1.3756964399126232 1103 48-49 1.305593960046732 1103 50-51 1.2230937006895175 1103 52-53 1.2893593327458 1103 54-55 1.1418377227387104 1103 56-57 1.1963068276243156 1103 58-59 1.183655491794248 1103 60-61 1.0053372837665542 1103 62-63 1.0056436436013314 1103 64-65 0.872603693994737 1103 66-67 -2.1098093623994743 1103 68-69 -6.855130379089278 1103 70-71 -10.759119301175593 1103 72-73 -8.120858731030498 1103 74-75 -6.006524483833157 1103 76-77 -2.8282388362691755 1103 78-79 -0.3922071217827927 1103 80-81 0.21554011128456452 1103 82-83 0.4257523961448797 1103 84-85 0.21302935802298606 1103 86-87 0.3730491016779496 1103 88-89 0.6021889045516389 1103 90-91 0.37660691083910436 1103 92-93 0.47377442999320607 1103 94-95 1.1899590617247426 1103 96-97 1.4536586280573616 1103 98-99 1.2629113461796813 1103 100-101 0.8427533278666353 1104 1 0.2572939642585439 1104 2 0.19655356879620456 1104 3 0.30857351108878106 1104 4 0.18151104485474434 1104 5 0.31960897207834194 1104 6 0.4572929205906746 1104 7 0.4520289898501275 1104 8 0.22433196086495144 1104 9 0.5995717987348286 1104 10-11 0.6653171415311618 1104 12-13 0.40692029006094543 1104 14-15 0.6629235943589507 1104 16-17 0.6052399104179074 1104 18-19 0.6852457192947625 1104 20-21 0.62853265694757 1104 22-23 0.5432006040159294 1104 24-25 0.7240806704244704 1104 26-27 0.9758498978073149 1104 28-29 0.8213535397901737 1104 30-31 0.7888888566543955 1104 32-33 0.4231752917314324 1104 34-35 0.5605457584986624 1104 36-37 0.5815420814662531 1104 38-39 0.5157391075805862 1104 40-41 0.7041450205681414 1104 42-43 0.6643523909943738 1104 44-45 0.5138879846572948 1104 46-47 0.2843638545694631 1104 48-49 0.28202480552704756 1104 50-51 0.34375111490636456 1104 52-53 0.5568938880059306 1104 54-55 0.46712250113876763 1104 56-57 0.4205198272534645 1104 58-59 0.43488492206459384 1104 60-61 0.32023210943455993 1104 62-63 0.4003050994793398 1104 64-65 0.25853303249486004 1104 66-67 -2.3836812093694846 1104 68-69 -6.627530209691507 1104 70-71 -8.299312576492547 1104 72-73 -7.585706335890553 1104 74-75 -6.329693361470767 1104 76-77 -3.336189178207537 1104 78-79 -0.9087475651083956 1104 80-81 -0.18019265194197587 1104 82-83 0.05772532235785022 1104 84-85 -0.18294933054282225 1104 86-87 0.036317598720561506 1104 88-89 -0.03309262103402588 1104 90-91 -0.09960963388630972 1104 92-93 0.09759253481240293 1104 94-95 0.9609214469333907 1104 96-97 0.9415976794298295 1104 98-99 0.7371614703209026 1104 100-101 0.5355589902779272 1105 1 0.4440896238727312 1105 2 0.4033313379312382 1105 3 0.426865359253064 1105 4 0.23093738275144204 1105 5 0.3880361656066569 1105 6 0.5218705497132632 1105 7 0.5680514696261056 1105 8 0.38463298762288645 1105 9 0.5982066773907135 1105 10-11 0.6554281791789549 1105 12-13 0.727618602133127 1105 14-15 0.9131563650496801 1105 16-17 0.9580421039462266 1105 18-19 0.9440838104584302 1105 20-21 0.8972721560141537 1105 22-23 1.0003758529269433 1105 24-25 0.9252878888438829 1105 26-27 1.1310851965005355 1105 28-29 1.1283868316383447 1105 30-31 1.0414938659885564 1105 32-33 1.119661368271565 1105 34-35 1.1389642012491237 1105 36-37 1.254337857757477 1105 38-39 1.3397722438593718 1105 40-41 1.5415437060068413 1105 42-43 1.4802185235394916 1105 44-45 1.605626278061159 1105 46-47 1.4933637378924232 1105 48-49 1.098990424693195 1105 50-51 1.1138639027097241 1105 52-53 1.3109313024427713 1105 54-55 1.2261780005164873 1105 56-57 1.2494444538869445 1105 58-59 1.5028568806831366 1105 60-61 1.350488798918068 1105 62-63 1.2796935173387034 1105 64-65 1.2237589970250404 1105 66-67 1.7346256123479975 1105 68-69 -4.791935934644837 1105 70-71 -5.069226624407914 1105 72-73 -1.5174527966870635 1105 74-75 1.0892141525304808 1105 76-77 1.0608394465591076 1105 78-79 1.046154620641449 1105 80-81 1.1883368284562827 1105 82-83 1.358015653720635 1105 84-85 1.382325443881573 1105 86-87 1.4591349602638068 1105 88-89 1.4308883995011321 1105 90-91 1.2073139815461715 1105 92-93 1.3586008188820955 1105 94-95 2.2750537586944404 1105 96-97 2.1747697391684717 1105 98-99 1.7007080633513958 1105 100-101 1.326175045038351 1106 1 0.17114922302885205 1106 2 0.17899476469434816 1106 3 0.14566282760749516 1106 4 0.03837220794734719 1106 5 0.21015039104366195 1106 6 0.2319541844329791 1106 7 0.2876381935561909 1106 8 0.2724637591720125 1106 9 0.313046189144778 1106 10-11 0.37833373222295563 1106 12-13 0.432055951439942 1106 14-15 0.42858565227089684 1106 16-17 0.26702906898540846 1106 18-19 0.3724644237797037 1106 20-21 0.2891607184011349 1106 22-23 0.48270971368643956 1106 24-25 0.544890058825807 1106 26-27 0.6176670982485746 1106 28-29 0.6664263132537727 1106 30-31 0.6395099901597447 1106 32-33 0.6929039089587263 1106 34-35 0.9615261738229677 1106 36-37 0.7309342637249259 1106 38-39 0.7162422559148212 1106 40-41 0.8101838883455983 1106 42-43 0.7267770678432939 1106 44-45 0.5596017527447046 1106 46-47 0.5449089081757279 1106 48-49 0.3414806296359352 1106 50-51 0.132919809882722 1106 52-53 0.5059670920750783 1106 54-55 0.5124236680873118 1106 56-57 0.5204484472564417 1106 58-59 0.5729399503033896 1106 60-61 0.4845861467179482 1106 62-63 0.673609468212728 1106 64-65 0.6586621766513225 1106 66-67 1.204355683173798 1106 68-69 -5.505377767074009 1106 70-71 -6.096535620791279 1106 72-73 -2.2718076414730817 1106 74-75 0.3286976516263209 1106 76-77 0.6422589763963593 1106 78-79 0.3951048542158908 1106 80-81 0.45821665063831674 1106 82-83 0.3262041332263621 1106 84-85 0.5872770336947113 1106 86-87 0.6379791435067226 1106 88-89 0.6898712204776274 1106 90-91 0.5155591593641375 1106 92-93 0.579002227863409 1106 94-95 1.3015045573683395 1106 96-97 1.640003313610908 1106 98-99 1.3831119964436205 1106 100-101 1.4999303192999562 1107 1 0.07680353982888377 1107 2 -0.03257076316498697 1107 3 0.023351351944903342 1107 4 0.008301116003579523 1107 5 0.11594420458351351 1107 6 0.11282213314810008 1107 7 0.061541877665085565 1107 8 0.0352891995595499 1107 9 0.2828367017512434 1107 10-11 0.1815240226625079 1107 12-13 0.12398088897844417 1107 14-15 0.2232998638316488 1107 16-17 0.08184310881833312 1107 18-19 0.240236748948071 1107 20-21 0.13101987221391198 1107 22-23 0.17876577375520242 1107 24-25 0.40668861965996683 1107 26-27 0.23597024522160837 1107 28-29 0.24506466355064305 1107 30-31 0.3386208452821009 1107 32-33 0.35229306741894817 1107 34-35 0.3834157085572869 1107 36-37 0.36055169758695627 1107 38-39 0.42026706724549 1107 40-41 0.31578358115909566 1107 42-43 0.2612287245139129 1107 44-45 0.2493286988894141 1107 46-47 0.3611001112176382 1107 48-49 0.481644200576266 1107 50-51 0.2625073253650214 1107 52-53 0.5012913816145108 1107 54-55 0.36027475752014126 1107 56-57 0.3066970421938855 1107 58-59 0.15396185936766926 1107 60-61 0.17769038235290324 1107 62-63 0.2351970952924205 1107 64-65 0.3104162747351502 1107 66-67 1.1081247749545753 1107 68-69 -5.17518806619173 1107 70-71 -5.519585181046164 1107 72-73 -2.0893776596590534 1107 74-75 0.17684737421135566 1107 76-77 0.22244115179662316 1107 78-79 0.14958526924072046 1107 80-81 0.01180744355981389 1107 82-83 0.003631137886287661 1107 84-85 0.0651029256111677 1107 86-87 0.1644912331018098 1107 88-89 0.08910703531112318 1107 90-91 0.19552500468868317 1107 92-93 0.2766021130355689 1107 94-95 1.1673413652717812 1107 96-97 1.3932563408737089 1107 98-99 1.0925982886863572 1107 100-101 1.0468693203124069 1108 1 0.03029608965104913 1108 2 -0.03414254153575058 1108 3 -0.04244717860278158 1108 4 -0.028717750501130013 1108 5 0.16028783126382962 1108 6 0.23056755153034914 1108 7 0.12859015829963738 1108 8 0.14574822081489458 1108 9 0.19255479974080458 1108 10-11 0.12717428002692088 1108 12-13 0.23337515119430208 1108 14-15 0.21117252949547094 1108 16-17 -0.03893889241960835 1108 18-19 0.10380575927732139 1108 20-21 0.11440608999355817 1108 22-23 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2.174508847577794 2316 98-99 1.2725096084375167 2316 100-101 1.1880595435185946 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 25.0 4 75.0 5 204.0 6 526.0 7 1197.0 8 2316.0 9 4025.0 10 6793.0 11 10180.0 12 12691.0 13 15891.0 14 19515.0 15 25705.0 16 30826.0 17 34458.0 18 27709.0 19 19287.0 20 17431.0 21 17209.0 22 18314.0 23 20205.0 24 23943.0 25 28988.0 26 35032.0 27 43180.0 28 52338.0 29 61874.0 30 72176.0 31 83825.0 32 96891.0 33 112419.0 34 131963.0 35 150499.0 36 148725.0 37 123977.0 38 58314.0 39 4610.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.006928035543034 20.168699846594443 15.77116200248119 18.053210115381336 2 15.601101618125544 23.461862057160154 39.223368474343495 21.713667850370808 3 20.268109305389807 29.819247253422564 31.1706968304503 18.741946610737322 4 11.8734876147959 18.79302574837875 37.10783711239657 32.22564952442878 5 11.478210074821405 41.49498756721074 31.842279677295938 15.184522680671918 6 26.74663558306208 38.15274578448437 17.786509028386256 17.3141096040673 7 25.07232356552204 38.34285533040207 19.37861865624203 17.206202447833864 8 26.70540223003718 32.77919407296982 19.22505084786082 21.290352849132184 9 28.260111092168437 17.40675249019056 20.561896945692236 33.77123947194877 10-11 24.546400077180376 28.569856833040603 24.699307094647725 22.184435995131295 12-13 28.695506225311203 26.788529727000842 26.19778265000945 18.318181397678508 14-15 24.67515518674612 26.749873458539998 28.82175032940427 19.753221025309614 16-17 19.024873590196993 30.789113030055532 29.330609542387258 20.85540383736021 18-19 18.699678025265683 27.839167920809743 35.156303948113994 18.304850105810576 20-21 20.9824972948863 25.76127268372058 34.48077510799805 18.775454913395063 22-23 19.868363842049828 26.301526823485567 34.145247129193876 19.684862205270733 24-25 19.637913010840936 26.505810334505576 35.218470691940595 18.637805962712893 26-27 20.052235711583542 25.99517951276405 35.663048272603675 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19.87040443972421 29.09333366839355 31.311178799153005 19.725083092729236 54-55 18.729725369257807 28.739359331586833 31.82510357997247 20.705811719182893 56-57 19.919558932551197 29.743595336943233 29.944582324845342 20.392263405660223 58-59 19.724883873012246 28.739483968907674 30.50079422697609 21.03483793110399 60-61 19.613879533295687 28.4504791646084 31.36422557000647 20.57141573208944 62-63 21.077997800179805 28.142499977697828 30.730510131629075 20.048992090493297 64-65 20.385952913149858 28.692911200432174 31.148359427530163 19.772776458887805 66-67 19.364958690405587 28.823708138129412 31.468994917069015 20.342338254395987 68-69 19.61939533005348 29.43238905189758 30.92303907092482 20.025176547124126 70-71 19.227114180191634 29.412691811979276 30.519113556423676 20.84108045140541 72-73 19.69480593692391 29.07741156477796 29.596668803323244 21.631113694974882 74-75 19.27879337691289 29.66113022321124 30.174874061957613 20.88520233791825 76-77 19.803037607769053 28.937438682626066 30.34422322184159 20.915300487763293 78-79 19.198688877764617 28.819643043006327 30.988083260227032 20.99358481900202 80-81 18.761915083000012 30.281276907024523 30.291420215117427 20.665387794858038 82-83 18.883983617671664 29.903398976302714 29.409920321831606 21.802697084194016 84-85 18.871924183493707 29.072190085757445 29.38837853803975 22.667507192709095 86-87 19.326448100900294 29.093482575410412 29.698140300019393 21.881929023669898 88-89 19.798893431492335 28.281660209591188 29.82613803890369 22.09330832001279 90-91 18.753659255421645 28.748432219686165 30.396996998584534 22.10091152630765 92-93 18.464616775346325 29.5132014727922 30.02459499536118 21.997586756500294 94-95 18.148261207559862 29.52983937077949 29.65982335950671 22.662076062153933 96-97 17.757590445692852 28.973379473284123 29.455504877538424 23.8135252034846 98-99 18.621601214698646 28.714901041382696 29.013255613768123 23.65024213015053 100-101 19.060134004124933 28.489027164945078 29.601708126354296 22.84913070457569 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9142.0 1 5977.5 2 3068.5 3 3365.0 4 3010.0 5 3012.5 6 4996.5 7 9510.5 8 13395.5 9 14314.5 10 12576.5 11 9934.5 12 8598.0 13 7990.5 14 7594.0 15 7190.5 16 6881.5 17 6759.5 18 6885.5 19 7235.5 20 7597.5 21 8028.0 22 8689.5 23 9544.5 24 10595.5 25 12032.5 26 13737.5 27 15634.5 28 17496.0 29 19531.0 30 21631.0 31 23765.0 32 26674.0 33 30060.0 34 33062.0 35 34889.5 36 36712.0 37 38735.0 38 39169.5 39 38779.5 40 38583.0 41 38721.0 42 39016.5 43 39389.0 44 40295.0 45 41523.5 46 44697.5 47 55642.5 48 83045.5 49 81724.5 50 54335.0 51 42990.5 52 36002.0 53 32090.0 54 29648.0 55 27338.0 56 25112.0 57 23670.5 58 22887.0 59 21565.0 60 20432.5 61 18797.0 62 15873.5 63 11857.5 64 8260.0 65 6233.5 66 4805.5 67 3540.0 68 2510.0 69 1853.0 70 1445.5 71 1198.0 72 1049.5 73 1078.0 74 911.5 75 659.0 76 493.0 77 283.0 78 176.5 79 133.0 80 88.5 81 53.5 82 34.0 83 23.0 84 15.5 85 9.5 86 7.5 87 5.0 88 1.5 89 1.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02345807744205194 2 0.019229015593343984 3 0.0 4 0.0 5 6.60790913860618E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.964745483163708E-4 18-19 0.0013546213734142668 20-21 3.30395456930309E-5 22-23 0.0 24-25 0.0 26-27 3.634350026233399E-4 28-29 0.018171750131166994 30-31 0.005154169128112821 32-33 0.009052835519890466 34-35 0.009911863707909271 36-37 0.1012662075491397 38-39 0.10301730347087035 40-41 0.11943795768030671 42-43 0.10248867073978185 44-45 0.09756577843152026 46-47 0.09915167662478573 48-49 0.1014644448232979 50-51 0.0999776652671115 52-53 0.07744469510446443 54-55 0.06475750955834057 56-57 0.036574777082185206 58-59 0.015297309655873309 60-61 0.008689400517267128 62-63 0.002147570470047009 64-65 0.004658575942717357 66-67 0.39250980283320713 68-69 0.7627839914150045 70-71 0.8444907879138699 72-73 0.2623670323483584 74-75 0.1373784309916225 76-77 0.013645332371221763 78-79 0.008623321425881066 80-81 0.0016519772846515452 82-83 0.0 84-85 0.0 86-87 1.6519772846515453E-4 88-89 7.268700052466798E-4 90-91 0.003436112752075214 92-93 0.0014537400104933597 94-95 0.2082812960488668 96-97 0.20814913786609468 98-99 0.013579253279835701 100-101 0.006673988229992243 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1513338.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.04529908561727 #Duplication Level Percentage of deduplicated Percentage of total 1 69.32034715428334 25.679929930492445 2 13.604571137227273 10.079708134202813 3 5.857167817523991 6.509416007844853 4 3.1706981995047157 4.698378524435214 5 1.9285601536120027 3.5722043847580314 6 1.2657611296986064 2.8134299772380196 7 0.937849445077988 2.432003923313598 8 0.6711657671991078 1.9890829265534977 9 0.5131830542582375 1.710991775760027 >10 2.499931818706822 16.031514422832117 >50 0.12633397431175372 3.210671785171843 >100 0.08881121406020052 6.365985200878099 >500 0.007083095894211847 1.8067605714824218 >1k 0.006719860207329187 5.077552945879027 >5k 9.08089217206647E-4 2.1856191690104443 >10k+ 9.08089217206647E-4 5.836750320147578 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24970 1.6499949119099633 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22184 1.4658985633083952 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15646 1.033873463826323 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12223 0.8076847340118334 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 11729 0.775041662867119 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9937 0.6566279311032962 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6993 0.4620910860627302 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5406 0.3572235680330501 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5108 0.33753199880000373 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5041 0.3331046996771376 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4723 0.3120915486163699 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4472 0.2955056966784684 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4366 0.2885013129915458 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4009 0.26491107736672176 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4008 0.2648449982753357 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3119 0.20610068603312678 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2871 0.18971307136938345 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2860 0.18898620136413677 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2521 0.16658538938426182 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2465 0.16288496026664234 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2315 0.1529730965587331 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2300 0.15198191018794216 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2206 0.14577047559765233 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2048 0.13532997915865458 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1998 0.1320260245893515 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1979 0.1307705218530163 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1964 0.1297793354822254 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1682 0.11114503171135595 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1673 0.1105503198888814 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1638 0.10823755169036924 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1566 0.10347985711057278 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.60790913860618E-5 0.0 2 0.0 0.0 0.0 6.60790913860618E-5 0.0 3 0.0 0.0 0.0 1.321581827721236E-4 0.0 4 0.0 0.0 0.0 1.321581827721236E-4 0.0 5 0.0 0.0 0.0 1.9823727415818542E-4 0.0 6 0.0 0.0 0.0 1.9823727415818542E-4 0.0 7 0.0 0.0 0.0 1.9823727415818542E-4 0.0 8 0.0 0.0 0.0 1.9823727415818542E-4 0.0 9 6.60790913860618E-5 0.0 0.0 3.30395456930309E-4 0.0 10-11 1.6519772846515453E-4 0.0 0.0 5.286327310884944E-4 0.0 12-13 1.9823727415818542E-4 0.0 0.0 6.60790913860618E-4 0.0 14-15 1.9823727415818542E-4 0.0 0.0 6.60790913860618E-4 0.0 16-17 1.9823727415818542E-4 0.0 0.0 7.599095509397108E-4 0.0 18-19 1.9823727415818542E-4 0.0 0.0 0.0011894236449491126 0.0 20-21 1.9823727415818542E-4 0.0 0.0 0.0015858981932654833 0.0 22-23 1.9823727415818542E-4 0.0 0.0 0.0028744404752936888 0.0 24-25 1.9823727415818542E-4 0.0 0.0 0.00512112958241979 0.0 26-27 1.9823727415818542E-4 0.0 0.0 0.007632135055090139 0.0 28-29 1.9823727415818542E-4 0.0 0.0 0.013645332371221761 0.0 30-31 2.9735591123727814E-4 0.0 0.0 0.030891975222983897 0.0 32-33 3.9647454831637084E-4 0.0 0.0 0.05365622220548219 0.0 34-35 3.9647454831637084E-4 0.0 0.0 0.0858367397104943 0.0 36-37 3.9647454831637084E-4 0.0 0.0 0.13338064596276575 0.0 38-39 3.9647454831637084E-4 0.0 0.0 0.20593548830466163 0.0 40-41 3.9647454831637084E-4 0.0 0.0 0.28866651072001104 0.0 42-43 5.286327310884944E-4 0.0 0.0 0.36465746581398206 0.0 44-45 5.286327310884944E-4 0.0 0.0 0.4520801037177418 0.0 46-47 5.286327310884944E-4 0.0 0.0 0.5597890226770226 0.0 48-49 5.286327310884944E-4 0.0 0.0 0.6845793867596004 0.0 50-51 5.286327310884944E-4 0.0 0.0 0.8251296141377538 0.0 52-53 5.286327310884944E-4 0.0 0.0 0.9548759100742861 0.0 54-55 5.947118224745563E-4 0.0 0.0 1.0858116296557676 0.0 56-57 5.947118224745563E-4 0.0 0.0 1.2281459925013447 0.0 58-59 5.947118224745563E-4 0.0 0.0 1.38125124724285 0.0 60-61 5.947118224745563E-4 0.0 0.0 1.5593343985282866 0.0 62-63 6.60790913860618E-4 0.0 0.0 1.7251268388159156 0.0 64-65 6.60790913860618E-4 0.0 0.0 1.8728796871551499 0.0 66-67 6.60790913860618E-4 0.0 0.0 2.026084060533734 0.0 68-69 6.60790913860618E-4 0.0 0.0 2.214211233709852 0.0 70-71 6.60790913860618E-4 0.0 0.0 2.4694747637342087 0.0 72-73 6.60790913860618E-4 0.0 0.0 2.7639231949505003 0.0 74-75 6.60790913860618E-4 0.0 0.0 3.0649795353053975 6.60790913860618E-5 76-77 6.60790913860618E-4 0.0 0.0 3.3618398533572806 6.60790913860618E-5 78-79 6.60790913860618E-4 0.0 0.0 3.692598745290213 6.60790913860618E-5 80-81 6.60790913860618E-4 0.0 0.0 4.071496255297891 6.60790913860618E-5 82-83 6.60790913860618E-4 0.0 0.0 4.472332023645742 6.60790913860618E-5 84-85 6.60790913860618E-4 0.0 0.0 4.894015745325895 6.60790913860618E-5 86-87 6.60790913860618E-4 0.0 0.0 5.31887126339258 6.60790913860618E-5 88-89 6.60790913860618E-4 0.0 0.0 5.733914036388434 6.60790913860618E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGACGC 65 2.675697E-7 29.659603 66-67 GTACATG 18880 0.0 23.97549 1 TAGCCCT 675 0.0 23.863842 4 TACATGG 18750 0.0 23.681215 2 GAGTACT 17460 0.0 23.404234 12-13 CATTCCG 290 3.6379788E-12 22.871532 9 TTAGGCA 415 0.0 22.832167 4 AGTACTT 17490 0.0 22.768133 12-13 GCGTAAT 75 2.7471266E-5 22.45488 62-63 ACATGGG 19150 0.0 22.142132 3 GTACTTT 18835 0.0 21.87175 14-15 ATGGGAG 3645 0.0 21.836195 5 GTATAGC 445 0.0 21.30277 1 CATGGGA 13535 0.0 20.966879 4 ATCGACG 70 4.4010466E-4 20.6551 64-65 CGTCACT 395 0.0 20.399426 1 ATGGGAT 4395 0.0 20.373615 5 ACTTTTT 20640 0.0 20.314491 16-17 TACTTTT 19635 0.0 20.304989 14-15 TAGGCAT 355 3.6379788E-12 20.018343 5 >>END_MODULE