FastQCFastQC Report
Fri 27 May 2016
SRR939518_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939518_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515172
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185651.2252734343031682No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137670.9086097155966452No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99230.6549091456283511No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA89230.5889100379362872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86440.5704962868902012No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA65850.4346041241522414No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32790.2164110741222779No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG29580.19522536055312534No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT28780.1899454319377602No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT28710.18948343818391575No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA28320.18690947298392527No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27920.1842695086762427No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27150.17918757738395377No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26440.17450164073781724No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG23330.15397591824558532No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC22890.1510719575071345No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20590.1358921627379598No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG18890.12467231443030889No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18730.12361632870723586No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG169200.026.6953451
TACATGG170600.026.138382
GAGTACT149100.025.550812-13
AGTACTT149800.024.65977912-13
ACATGGG178300.023.9062923
GTACTTT163450.023.62594214-15
CATGGGG47300.023.2880334
CGTCACT3950.023.075251
ACTTTTT190100.022.47647516-17
ATGGGAG35350.021.745535
CATGGGT13650.021.659134
TACTTTT174450.021.45634314-15
CAACGCG2959.640644E-1121.4399455
CATGGGA126450.021.3441834
GTATCAA392500.021.0548211
CTCAACG7450.020.4126432
ATGGGGG21200.018.8182985
TACCTGG8350.018.7999762
ATGGGAT40900.018.675785
GCTTATA15600.018.5021111
Produced by FastQC (version 0.11.5)